A Role for Sodium-Glucose Cotransporter 2 Inhibitors in the Treatment of Chronic Kidney Disease: A Mini Review

Background: Sodium-glucose cotransport protein 2 (SGLT2) inhibitors, a new type of glucose-lowering drug, have been well proved in several clinical studies for their glucose-lowering and nephroprotective effects, and the nephroprotective effects include both indirect effects of metabolic improvement and direct effects, independent of glucose-lowering effects. Summary: In patients with diabetic kidney disease (DKD), several studies have demonstrated the potential nephroprotective mechanisms of SGLT2 inhibitors, and evidence of nephroprotective mechanisms in the non-DKD population is accumulating. Although the nephroprotective mechanism of SGLT2 inhibitors has not been fully elucidated, several laboratory studies have illustrated the mechanism underlying the effects of SGLT2 inhibitors at various aspects. Key Messages: The purpose of this article is to review the mechanism of nephroprotective effect of SGLT2 inhibitors and to look forward to promising research in the future

An efficient approach of secure group association management in densely deployed heterogeneous distributed sensor network

A heterogeneous distributed sensor network (HDSN) is a type of distributed sensor network where sensors with different deployment groups and different functional types participate at the same time. In other words, the sensors are divided into different deployment groups according to different types of data transmissions, but they cooperate with each other within and out of their respective groups. However, in traditional heterogeneous sensor networks, the classification is based on transmission range, energy level, computation ability, and sensing range. Taking this model into account, we propose a secure group association authentication mechanism using one-way accumulator which ensures that: before collaborating for a particular task, any pair of nodes in the same deployment group can verify the legitimacy of group association of each other. Secure addition and deletion of sensors are also supported in this approach. In addition, a policy-based sensor addition procedure is also suggested. For secure handling of disconnected nodes of a group, we use an efficient pairwise key derivation scheme to resist any adversary’s attempt. Along with proposing our mechanism, we also discuss the characteristics of HDSN, its scopes, applicability, future, and challenges. The efficiency of our security management approach is also demonstrated with performance evaluation and analysis

Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water extractable colloids of a Vertisol

Water extractable colloids (WECs) serve as crucial micro particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the microparticle scale under long-term straw retention and mineral fertilization is barely known. Here, a fixed field experiment (~13 years) in a Vertisol was performed to explore the impacts of straw retention and mineral fertilization on inorganic P, organic P and P-transformation microorganisms in bulk soils and WECs by sequential extraction procedure, P K-edge X-ray absorptions near-edge structure (XANES), 31P nuclear magnetic resonance (NMR), and metagenomics analysis. In bulk soil, mineral fertilization led to increases in the levels of total P, available P, acid phosphatase (ACP), high-activity inorganic P fractions (Ca2-P, Ca8-P, Al-P, and Fe-P) and organic P (orthophosphate monoesters and orthophosphate diesters), but significantly decreased the abundances of P cycling genes including P mineralization, P-starvation response regulation, P-uptake and transport by decreasing soil pH and increasing P in bulk soil. Straw retention had no significant effects on P species and P-transformation microorganisms in bulk soils but brought increases for organic carbon, total P, available P concentrations in WECs. Furthermore, straw retention caused greater change in P cycling genes between WECs and bulk soils compared with the effect of mineral fertilization. The abundances of phoD gene and phoD-harbouring Proteobacteria in WECs increased significantly under straw retention, suggesting that the P mineralizing capacity increased. Thus, straw retention could potentially accelerate the turnover, mobility and availability of P by increasing the nutrient contents and P mineralizing capacity in microscopic colloidal scale

Path Planning for Autonomous Vehicle Based on a Two-Layered Planning Model in Complex Environment

The autonomous vehicle consists of perception, decision-making, and control system. The study of path planning method has always been a core and difficult problem, especially in complex environment, due to the effect of dynamic environment, the safety, smoothness, and real-time requirement, and the nonholonomic constraints of vehicle. To address the problem of travelling in complex environments which consists of lots of obstacles, a two-layered path planning model is presented in this paper. This method includes a high-level model that produces a rough path and a low-level model that provides precise navigation. In the high-level model, the improved Bidirectional Rapidly-exploring Random Tree (Bi-RRT) based on the steering constraint is used to generate an obstacle-free path while satisfying the nonholonomic constraints of vehicle. In low-level model, a Vector Field Histogram- (VFH-) guided polynomial planning algorithm in Frenet coordinates is introduced. Based on the result of VFH, the aim point chosen from improved Bi-RRT path is moved to the most suitable location on the basis of evaluation function. By applying quintic polynomial in Frenet coordinates, a real-time local path that is safe and smooth is generated based on the improved Bi-RRT path. To verify the effectiveness of the proposed planning model, the real autonomous vehicle has been placed in several driving scenarios with different amounts of obstacles. The two-layered real-time planning model produces flexible, smooth, and safe paths that enable the vehicle to travel in complex environment. Document type: Articl

Localization Algorithms of Underwater Wireless Sensor Networks: A Survey

In Underwater Wireless Sensor Networks (UWSNs), localization is one of most important technologies since it plays a critical role in many applications. Motivated by widespread adoption of localization, in this paper, we present a comprehensive survey of localization algorithms. First, we classify localization algorithms into three categories based on sensor nodes’ mobility: stationary localization algorithms, mobile localization algorithms and hybrid localization algorithms. Moreover, we compare the localization algorithms in detail and analyze future research directions of localization algorithms in UWSNs

Structural and Functional Analysis of Laninamivir and its Octanoate Prodrug Reveals Group Specific Mechanisms for Influenza NA Inhibition

The 2009 H1N1 influenza pandemic (pH1N1) led to record sales of neuraminidase (NA) inhibitors, which has contributed significantly to the recent increase in oseltamivir-resistant viruses. Therefore, development and careful evaluation of novel NA inhibitors is of great interest. Recently, a highly potent NA inhibitor, laninamivir, has been approved for use in Japan. Laninamivir is effective using a single inhaled dose via its octanoate prodrug (CS-8958) and has been demonstrated to be effective against oseltamivir-resistant NA in vitro. However, effectiveness of laninamivir octanoate prodrug against oseltamivir-resistant influenza infection in adults has not been demonstrated. NA is classified into 2 groups based upon phylogenetic analysis and it is becoming clear that each group has some distinct structural features. Recently, we found that pH1N1 N1 NA (p09N1) is an atypical group 1 NA with some group 2-like features in its active site (lack of a 150-cavity). Furthermore, it has been reported that certain oseltamivir-resistant substitutions in the NA active site are group 1 specific. In order to comprehensively evaluate the effectiveness of laninamivir, we utilized recombinant N5 (typical group 1), p09N1 (atypical group 1) and N2 from the 1957 pandemic H2N2 (p57N2) (typical group 2) to carry out in vitro inhibition assays. We found that laninamivir and its octanoate prodrug display group specific preferences to different influenza NAs and provide the structural basis of their specific action based upon their novel complex crystal structures. Our results indicate that laninamivir and zanamivir are more effective against group 1 NA with a 150-cavity than group 2 NA with no 150-cavity. Furthermore, we have found that the laninamivir octanoate prodrug has a unique binding mode in p09N1 that is different from that of group 2 p57N2, but with some similarities to NA-oseltamivir binding, which provides additional insight into group specific differences of oseltamivir binding and resistance

Transcriptome Analysis of H2O2-Treated Wheat Seedlings Reveals a H2O2-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance

Hydrogen peroxide (H2O2) plays important roles in plant biotic and abiotic stress responses. However, the effect of H2O2 stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H2O2, we performed an mRNA tag analysis of wheat seedlings under 10 mM H2O2 treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H2O2 responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H2O2-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H2O2-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H2O2 and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H2O2 stress and uncovers potential links between H2O2 and Bgt responses, hence providing important candidate genes for the PM resistance in wheat