First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean

Citation: Yang Y, Kelly PJ, Bai J, Zhang R, Wang C (2016) First Molecular Characterization of Bovine Leukemia Virus Infections in the Caribbean. PLoS ONE 11(12): e0168379. doi:10.1371/journal.pone.0168379Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis. To investigate the presence and genetic variability of BLV in the Caribbean for the first time, we preformed fluorescence resonance energy transfer (FRET)-PCR for the pol of BLV on DNA from whole blood of cattle from Dominica, Montserrat, Nevis and St. Kitts. Standard PCRs with primers for the env were used for phylogenetic analysis of BLV in positive animals. We found FRET-PCR positive cattle (12.6%, 41/325) on Dominica (5.2%; 4/77) and St. Kitts (19.2%; 37/193) but not on Montserrat (0%, 0/12) or Nevis (0%, 0/43). Positive animals were cows on farms where animals were raised intensively. Phylogenetic analysis using the neighbor-joining (NJ) method on partial and full-length env sequences obtained for strains from Dominica (n = 2) and St. Kitts (n = 5) and those available in GenBank (n = 90) (genotypes 1–10) revealed the Caribbean strains belonged to genotype 1 (98–100% sequence homology). Ours is the first molecular characterization of BLV infections in the Caribbean and the first description of genotype 1 in the region

Los Angeles Metro Bus Data Analysis Using GPS Trajectory and Schedule Data (Demo Paper)

With the widespread installation of location-enabled devices on public transportation, public vehicles are generating massive amounts of trajectory data in real time. However, using these trajectory data for meaningful analysis requires careful considerations in storing, managing, processing, and visualizing the data. Using the location data of the Los Angeles Metro bus system, along with publicly available bus schedule data, we conduct a data processing and analyses study to measure the performance of the public transportation system in Los Angeles utilizing a number of metrics including travel-time reliability, on-time performance, bus bunching, and travel-time estimation. We demonstrate the visualization of the data analysis results through an interactive web-based application. The developed algorithms and system provide powerful tools to detect issues and improve the efficiency of public transportation systems.Comment: SIGSPATIAL'18, demo paper, 4 page

CO-CHANGES I: IRAM 30m CO Observations of Molecular Gas in the Sombrero Galaxy

Molecular gas plays a critical role in explaining the quiescence of star formation (SF) in massive isolated spiral galaxies, which could be a result of either the low molecular gas content and/or the low SF efficiency. We present IRAM 30m observations of the CO lines in the Sombrero galaxy (NGC~4594), the most massive spiral at $d\lesssim30\rm~Mpc$. We detect at least one of the three CO lines covered by our observations in all 13 observed positions located at the galactic nucleus and along a $\sim25\rm~kpc$-diameter dusty ring. The total extrapolated molecular gas mass of the galaxy is $M_{\rm H_2}\approx4\times10^{8}\rm~M_\odot$. The measured maximum CO gas rotation velocity of $\approx379\rm~km~s^{-1}$ suggests that NGC~4594 locates in a dark matter halo with a mass $M_{\rm200}\gtrsim10^{13}\rm~M_\odot$. Comparing to other galaxy samples, NGC~4594 is extremely gas poor and SF inactive, but the SF efficiency is apparently not inconsistent with that predicted by the Kennicutt-Schmidt law, so there is no evidence of enhanced SF quenching in this extremely massive spiral with a huge bulge. We also calculate the predicted gas supply rate from various sources to replenish the cold gas consumed in SF, and find that the galaxy must experienced a starburst stage at high redshift, then the leftover or recycled gas provides SF fuels to maintain the gradual growth of the galactic disk at a gentle rate.Comment: 21 pages, 13 figures, accepted for publication in MNRA

Fracturing fluid flow characteristics in shale gas matrix-fracture system based on NMR method

To understand the occurrence state of fracturing fluid in shale gas matrix-fracture system, an experimental method for evaluating fracturing fluid flow characteristics in matrix-fracture system was established. By using Nuclear Magnetic Resonance method, the flow characteristics of fracturing fluid were investigated from three processes of filtration, well shut-in and flowback. The T2 spectrum of fracturing fluid flow process and fracturing fluid saturation in matrix-fracture core model were clarified. The results demonstrate that the peak area of T2 spectra increases gradually during the filtration process, and the fracturing fluid quickly fills the fractures and matrix pores. During the well shut-in process, the fracturing fluid gradually flows from the fracture space to the matrix pores, and the signal of the matrix pores increases by 50.5%. During the flowback process, fracturing fluid flows out of the matrix and fracture. And when it reaches a stable state, the peak signal in the fracture decreases by 64.5% and the matrix signal reduces by 18.8%. The better the porosity and permeability characteristics of the core, the more likely the fracturing fluid is to stay in the formation and cannot be discharged. This paper would contribute to basic parameters for shale gas fracturing design and production strategy optimization

Observation of Flat Band and Van Hove Singularity in Non-superconducting Nitrogen-doped Lutetium Hydride

Hydrogen-rich materials offer a compelling avenue towards room temperature superconductivity, albeit under ultra-high pressure. However, the experimental investigation of the electronic band structure remains elusive, due to the inherent instability of most of the hydrogen-rich materials upon pressure release. Very recently, nitrogen-doped lutetium hydride was claimed to host room temperature superconductivity under near ambient pressure but was disproven by following works. Upon decompression, nitrogen doped lutetium hydride manifests a stable metallic phase with dark blue color. Moreover, high temperature superconductivity has been reported in lutetium hydrides Lu4H23 (~71 K) under around 200 GPa. These properties engender an unprecedented opportunity, allowing for the experimental investigation of the electronic band structure intrinsic to hydrogen-rich material. In this work, using angle resolved photoemission spectroscopy to investigate the non-superconducting nitrogen doped lutetium hydride, we observed significant flat band and Van Hove singularity marginally below the Fermi level. These salient features, identified as critical elements, proffer potential amplifiers for the realization of heightened superconductivity, as evidenced by prior research. Our results not only unveil a confluence of potent strong correlation effects and anisotropy within the Lu-H-N compound, but also provide a prospect for engineering high temperature superconductivity through the strategic manipulation of flat band and the VHS, effectively tailoring their alignment with the Fermi energy.Comment: 26 pages, 9 figure