Expresión incompleta de incompatibilidad trimórfica en Oxalis compacta Gill. ex Hook. et Arn. subsp. compacta en los Andes de Chile central

The expression of trimorphic incompatibility was investigated in a high altitude population of Oxalis compacta subsp.compacta distributed in the upper alpine belt (3100-3470 m) of the central Chile Andes. Stigma-anther reciprocity (2populations), morph representation (7 populations), unassisted selfing capacity and open-pollination fruit set (6 populations)were determined. O. compacta ssp. compacta es highly dependent on external pollinators. Although it has typical tristylousflowers, considerable seed set is possible following illegitimate intermorph and intramorph cross pollination. Openpollination fruit set varied from 9-83% and on average was lower in the higher-elevation populations. The possibilityof seed set following two kinds of illegitimate cross pollination is seen as a means of enhancing reproductive output byenabling more effective use of the limited pollinator resource in the high alpine environment, where fruit set shows highinter-annual variability and there is some evidence for pollination limitation in Oxalis compacta subsp. compacta.Se investigó la expresión de incompatibilidad trimórfica en una población de Oxalis compacta subsp. compacta distribuidaen la zona alpina superior de los Andes de Chile central. Se determinó la reciprocidad de los estigmas y anteras (dospoblaciones), representación de morfos florales (7 poblaciones), capacidad para autofecundación no asistida y polinizaciónabierta (6 poblaciones). O. compacta subsp. compacta es altamente dependiente de polinizadores externos. No obstantela presencia de flores tristilicas, las dos categorías ilegítimas de polinización dieron semillas. La producción de frutosmediante polinización abierta fluctuó entre 9-83% y en promedio fue menor en las poblaciones de mayor elevación. Seplantea que la capacidad de formar semillas mediante polinización ilegítima permitirá el uso más eficaz del recurso limitadode polinización en la zona andina superior, donde los niveles de fructificación varían entre años y existe evidencia preliminarde limitación de polen en Oxalis compacta subsp. compacta

Incomplete trimorphic incompatibility expression in Oxalis compacta Gill. ex Hook. et Arn. subsp. compacta in the central Chilean Andes Expresión incompleta de incompatibilidad trimórfica en Oxalis compacta Gill. ex Hook. et Arn. subsp. compacta en los Andes de Chile central

The expression of trimorphic incompatibility was investigated in a high altitude population of Oxalis compacta subsp. compacta distributed in the upper alpine belt (3100-3470 m) of the central Chile Andes. Stigma-anther reciprocity (2 populations), morph representation (7 populations), unassisted selfing capacity and open-pollination fruit set (6 populations) were determined. O. compacta ssp. compacta es highly dependent on external pollinators. Although it has typical tristylous flowers, considerable seed set is possible following illegitimate intermorph and intramorph cross pollination. Open pollination fruit set varied from 9-83% and on average was lower in the higher-elevation populations. The possibility of seed set following two kinds of illegitimate cross pollination is seen as a means of enhancing reproductive output by enabling more effective use of the limited pollinator resource in the high alpine environment, where fruit set shows high inter-annual variability and there is some evidence for pollination limitation in Oxalis compacta subsp. compacta.Se investigó la expresión de incompatibilidad trimórfica en una población de Oxalis compacta subsp. compacta distribuida en la zona alpina superior de los Andes de Chile central. Se determinó la reciprocidad de los estigmas y anteras (dos poblaciones), representación de morfos florales (7 poblaciones), capacidad para autofecundación no asistida y polinización abierta (6 poblaciones). O. compacta subsp. compacta es altamente dependiente de polinizadores externos. No obstante la presencia de flores tristilicas, las dos categorías ilegítimas de polinización dieron semillas. La producción de frutos mediante polinización abierta fluctuó entre 9-83% y en promedio fue menor en las poblaciones de mayor elevación. Se plantea que la capacidad de formar semillas mediante polinización ilegítima permitirá el uso más eficaz del recurso limitado de polinización en la zona andina superior, donde los niveles de fructificación varían entre años y existe evidencia preliminar de limitación de polen en Oxalis compacta subsp. compacta

Temperature-driven flower longevity in a high-alpine species of Oxalis influences reproductive assurance

How high-alpine plants confront stochastic conditions for animal pollination is a critical question. We investigated the effect of temperature on potential flower longevity (FL) measured in pollinator-excluded flowers and actual FL measured in pollinated flowers in self-incompatible Oxalis compacta and evaluated if plastically prolonged potential FL can ameliorate slow pollination under cool conditions. Pollinator-excluded and hand-pollinated flowers were experimentally warmed with open-top chambers (OTCs) on a site at 3470 m above sea level (asl). Flower-specific temperatures, and pollinator-excluded and open-pollination flower life-spans were measured at six alpine sites between 3100 and 3470 m asl. Fruit set was analyzed in relation to inferred pollination time. Warming reduced potential FL. Variable thermal conditions across the alpine landscape predicted potential and actual FL; flower senescence was pollination-regulated. Actual FL and potential FL were coupled. Prolonged potent

NDVI changes in the Arctic: Functional significance in the moist acidic tundra of Northern Alaska.

The Normalized Difference Vegetation Index (NDVI), derived from reflected visible and infrared radiation, has been critical to understanding change across the Arctic, but relatively few ground truthing efforts have directly linked NDVI to structural and functional properties of Arctic tundra ecosystems. To improve the interpretation of changing NDVI within moist acidic tundra (MAT), a common Arctic ecosystem, we coupled measurements of NDVI, vegetation structure, and CO2 flux in seventy MAT plots, chosen to represent the full range of typical MAT vegetation conditions, over two growing seasons. Light-saturated photosynthesis, ecosystem respiration, and net ecosystem CO2 exchange were well predicted by NDVI, but not by vertically-projected leaf area, our nondestructive proxy for leaf area index (LAI). Further, our data indicate that NDVI in this ecosystem is driven primarily by the biochemical properties of the canopy leaves of the dominant plant functional types, rather than purely the amount of leaf area; NDVI was more strongly correlated with top cover and repeated cover of deciduous shrubs than other plant functional types, a finding supported by our data from separate "monotypic" plots. In these pure stands of a plant functional type, deciduous shrubs exhibited higher NDVI than any other plant functional type. Likewise, leaves from the two most common deciduous shrubs, Betula nana and Salix pulchra, exhibited higher leaf-level NDVI than those from the codominant graminoid, Eriophorum vaginatum. Our findings suggest that recent increases in NDVI in MAT in the North American Arctic are largely driven by expanding deciduous shrub canopies, with substantial implications for MAT ecosystem function, especially net carbon uptake

Resistance and change in a High Arctic ecosystem, NW Greenland:differential sensitivity of ecosystem metrics to 15 years of experimental warming and wetting

Abstract Dramatic increases in air temperature and precipitation are occurring in the High Arctic (>70 °N), yet few studies have characterized the long-term responses of High Arctic ecosystems to the interactive effects of experimental warming and increased rain. Beginning in 2003, we applied a factorial summer warming and wetting experiment to a polar semidesert in northwest Greenland. In summer 2018, we assessed several metrics of ecosystem structure and function, including plant cover, greenness, ecosystem CO₂ exchange, aboveground (leaf, stem) and belowground (litter, root, soil) carbon (C) and nitrogen (N) concentrations (%) and pools, as well as leaf and soil stable isotopes (δ¹³C and δ¹⁵N). Wetting induced the most pronounced changes in ecosystem structure, accelerating the expansion of S. arctica cover by 370% and increasing aboveground C, N, and biomass pools by 94–101% and root C, N, and biomass pools by 60–122%, increases which coincided with enhanced net ecosystem CO₂ uptake. Further, wetting combined with warming enhanced plot-level greenness, whereas in isolation neither wetting nor warming had an effect. At the plant level the effects of warming and wetting differed among species and included warming-linked decreases in leaf N and δ¹⁵N in Salix arctica, whereas leaf N and δ¹⁵N in Dryas integrifolia did not respond to the climate treatments. Finally, neither plant- nor plot-level C and N allocation patterns nor soil C, N, δ¹³C, or δ¹⁵N concentrations changed in response to our manipulations, indicating that these ecosystem metrics may resist climate change, even in the longer term. In sum, our results highlight the importance of summer precipitation in regulating ecosystem structure and function in arid parts of the High Arctic, but they do not completely refute previous findings of resistance in some High Arctic ecosystem properties to climate change

NDVI changes in the Arctic:functional significance in the moist acidic tundra of Northern Alaska

Abstract The Normalized Difference Vegetation Index (NDVI), derived from reflected visible and infrared radiation, has been critical to understanding change across the Arctic, but relatively few ground truthing efforts have directly linked NDVI to structural and functional properties of Arctic tundra ecosystems. To improve the interpretation of changing NDVI within moist acidic tundra (MAT), a common Arctic ecosystem, we coupled measurements of NDVI, vegetation structure, and CO₂ flux in seventy MAT plots, chosen to represent the full range of typical MAT vegetation conditions, over two growing seasons. Light-saturated photosynthesis, ecosystem respiration, and net ecosystem CO₂ exchange were well predicted by NDVI, but not by vertically-projected leaf area, our nondestructive proxy for leaf area index (LAI). Further, our data indicate that NDVI in this ecosystem is driven primarily by the biochemical properties of the canopy leaves of the dominant plant functional types, rather than purely the amount of leaf area; NDVI was more strongly correlated with top cover and repeated cover of deciduous shrubs than other plant functional types, a finding supported by our data from separate “monotypic” plots. In these pure stands of a plant functional type, deciduous shrubs exhibited higher NDVI than any other plant functional type. Likewise, leaves from the two most common deciduous shrubs, Betula nana and Salix pulchra, exhibited higher leaf-level NDVI than those from the codominant graminoid, Eriophorum vaginatum. Our findings suggest that recent increases in NDVI in MAT in the North American Arctic are largely driven by expanding deciduous shrub canopies, with substantial implications for MAT ecosystem function, especially net carbon uptake

Impact of glaucoma medications on the ocular surface and how ocular surface disease can influence glaucoma treatment

Glaucoma is a common disease with an increasing prevalence [1]. Ocular surface disease (OSD) is also common, and its prevalence is increasing [2,3], due in part to the adverse effects of topical glaucoma medications [4,5]. Given this glaucoma/OSD association, David A. Sullivan, MS, PhD (Boston, MA, USA) and Amy Gallant Sullivan (Paris, France) on behalf of the Tear Film & Ocular Surface Society (TFOS), and in collaboration with Miriam Kolko, MD, PhD (Copenhagen University Hospital & University of Copenhagen, Denmark), organized a one-day meeting which was held on Saturday, October 22, in Cernobbio, Italy. This meeting focused on the impact of glaucoma medications on the ocular surface, and how OSD can influence glaucoma treatment. The term "ocular surface" encompasses the surface (cornea and conjunctiva), tear film, and adnexa (lacrimal and meibomian glands). The speakers included internationally renowned glaucoma and OSD experts. The evidence-based proceedings of this meeting are presented in this TFOS Experts' Meeting report

Impact of glaucoma medications on the ocular surface and how ocular surface disease can influence glaucoma treatment

International audienc