BIO-R in the postoperative period after extracapsular senile cataract extraction with implantation of intraocular lens

Catedra Oftalmologie USMF “Nicolae Testemiţanu”64 patients (aged 55-75 years) have been investigated after extracapsular senile cataract extraction with implantation of intraocular lenses. Sol. Dexamethason (1,0 ml subconjunctivally) has been administrated in 32 patients, and BIO-R (0,5 ml subconjunctivally) in other 32 patients. Degenerative modifications of conjunctiva and cornea were less expressed in patients who recieved BIO-R. În studiu au fost incluşi 64 pacienţi cu vârsta de 55-75 ani după extracţia extracapsulară a cataractei senile cu lentile intraoculare. La 32 pacienţi în postoperator s-a administrat Sol. Dexametazonă – 1,0 ml subconjunctival. La 32 pacienţi în perioada dată s-a administrat Sol. BIO-R – 0,5 ml subconjunctival. Procese degenerative mai diminuate ale conjnctivei şi corneei s-au determinat la administrarea BIO-Rului

Reimplantarea de cristalin toric la un pacient cu astigmatism, artifakie, sechelele traumatismului ocular

Introducere: Astigmatismul rămâne o problemă actuală a pacienților cu vârsta activă, astfel chirurgia refractivă a cristalinului reprezintă o opțiune eligibilă de tratament. Scopul lucrării. Evaluarea utilizării chirurgiei refractive a cristalinului cu reimplantare de IOL toric în corecția astigmatismului la un pacient cu artifakie. Material și Metode. Lucrarea prezintă un caz clinic a unui pacient de 23 ani care a suferit un traumatism ocular penetrant în 2017, i s-a efectuat EEC prin Facoemulsificare +PF monofocal. Peste 5 ani a fost consultat în cadru CM ,,Ovisus” cu acuze la scăderea funcțiilor vizuale. Preoperator Vis OD=0,09cccyl -4,0ax 180=0,3. S-a calculat cristalin toric monofocal SN6AT9 pentru refracția postoperatorie -2,5.Calculele au fost efectuate utilizând formulele Barrett și Hollday I. A fost efectuată intervenția chirurgicală de înlaturarea pseudofakului monofocal și reimplantarea pseudofakului toric SN6AT9. Rezultate:Perioada postoperatorie a decurs fără complicații.A doua zi postoperator Vis ODcc2,5=0,3. La 1 luna postoperator Vis OD cc-2,5D=0,5. S-a atins refracția postoperatorie țintă. Concluzii. Implantarea lentilelor intraoculare torice este o metodă eficientă de corecție a astigmatismului clinic manifest

Periodic fever syndromes in Eastern and Central European countries: results of a pediatric multinational survey

<p>Abstract</p> <p>Objective</p> <p>To analyze the prevalence of diagnosed and suspected autoinflammatory diseases in Eastern and Central European (ECE) countries, with a particular interest on the diagnostic facilities in these countries.</p> <p>Methods</p> <p>Two different strategies were used to collect data on patients with periodic fever syndromes from ECE countries- the Eurofever survey and collection of data with the structured questionnaire.</p> <p>Results</p> <p>Data from 35 centers in 14 ECE countries were collected. All together there were 11 patients reported with genetically confirmed familial Mediterranean fever (FMF), 14 with mevalonate-kinase deficiency (MKD), 11 with tumor necrosis factor receptor associated periodic syndrome (TRAPS) and 4 with chronic infantile neurological cutaneous and articular syndrome (CINCA). Significantly higher numbers were reported for suspected cases which were not genetically tested. All together there were 49 suspected FMF patients reported, 24 MKD, 16 TRAPS, 7 CINCA and 2 suspected Muckle-Wells syndrome (MWS) patients.</p> <p>Conclusions</p> <p>The number of genetically confirmed patients with periodic fever syndromes in ECE countries is very low. In order to identify more patients in the future, it is important to organize educational programs for increasing the knowledge on these diseases and to establish a network for genetic testing of periodic fever syndromes in ECE countries.</p

European lipodystrophy registry: Background and structure

Background: Lipodystrophy syndromes comprise a group of extremely rare and heterogeneous diseases characterized by a selective loss of adipose tissue in the absence of nutritional deprivation or catabolic state. Because of the rarity of each lipodystrophy subform, research in this area is difficult and international co-operation mandatory. Therefore, in 2016, the European Consortium of Lipodystrophies (ECLip) decided to create a registry for patients with lipodystrophy. Results: The registry was build using the information technology Open Source Registry System for Rare Diseases in the EU (OSSE), an open-source software and toolbox. Lipodystrophy specific data forms were developed based on current knowledge of typical signs and symptoms of lipodystrophy. The platform complies with the new General Data Protection Regulation (EU) 2016/679 by ensuring patient pseudonymization, informational separation of powers, secure data storage and security of communication, user authentication, person specific access to data, and recording of access granted to any data. Inclusion criteria are all patients with any form of lipodystrophy (with the exception of HIV-associated lipodystrophy). So far 246 patients from nine centres (Amsterdam, Bologna, Izmir, Leipzig, M\ufcnster, Moscow, Pisa, Santiago de Compostela, Ulm) have been recruited. With the help from the six centres on the brink of recruitment (Cambridge, Lille, Nicosia, Paris, Porto, Rome) this number is expected to double within the next one or 2 years. Conclusions: A European registry for all patients with lipodystrophy will provide a platform for improved research in the area of lipodystrophy. All physicians from Europe and neighbouring countries caring for patients with lipodystrophy are invited to participate in the ECLip Registry. Study registration: ClinicalTrials.gov (NCT03553420). Registered 14 March 2018, retrospectively registered

Cytokine Signature in Schnitzler Syndrome: Proinflammatory Cytokine Production Associated to Th Suppression

Background: Schnitzler&nbsp;syndrome (SchS) is a rare autoinflammatory disease characterized by urticarial exanthema, bone and joint alterations, fever and monoclonal IgM gammopathy. Overactivation of the&nbsp;interleukin(IL)-1 system is reported, even though the exact pathophysiological pathways remain unknown. Objective: To determine ex vivo cytokine profiles of Peripheral Blood Mononuclear Cells (PBMCs) from SchS patients prior to treatment and after initiation of anti-IL-1 therapy (anakinra). The sera cytokine profile was studied in parallel. Methods: We collected blood samples from thirty-six untreated or treated SchS. PBMCs were cultured with and without LPS or anti-CD3/CD28. Cytokine levels were evaluated in serum and cell culture supernatants using Luminex technology. Results: Spontaneous TNFα, IL-6, IL-1β, IL-1α, and IL-1RA release by PBMCs of SchS patients were higher than in controls. LPS-stimulation further induced the secretion of these cytokines. In contrast, after T-cell stimulation, TNFα, IL-10, IFNγ, IL-17A, and IL-4 production decreased in SchS patients compared to healthy controls, but less in treated patients. Whereas IL-1β serum level was not detected in most sera, IL-6, IL-10, and TNFα serum levels were higher in patients with SchS and IFNγ and IL-4 levels were lower. Of note, IL-6 decreased after treatment in SchS (p = 0.04). Conclusion: Our data strengthen the hypothesis of myeloid inflammation in SchS, mediated in particular by IL-1β, TNFα, and IL-6, associated with overproduction of the inhibitors IL-1RA and IL-10. In contrast, we observed a loss of Th1, Th2, and Th17 cell functionalities that tends to be reversed by anakinra

Pattern recognition receptors in immune disorders affecting the skin.

Contains fulltext : 109004.pdf (publisher's version ) (Open Access)Pattern recognition receptors (PRRs) evolved to protect organisms against pathogens, but excessive signaling can induce immune responses that are harmful to the host. Putative PRR dysfunction is associated with numerous immune disorders that affect the skin, such as systemic lupus erythematosus, cryopyrin-associated periodic syndrome, and primary inflammatory skin diseases including psoriasis and atopic dermatitis. As yet, the evidence is often confined to genetic association studies without additional proof of a causal relationship. However, insight into the role of PRRs in the pathophysiology of some disorders has already resulted in new therapeutic approaches based on immunomodulation of PRRs

The regulation of MEFV expression and its role in health and familial Mediterranean fever

Familial Mediterranean fever (FMF) is a hereditary recurrent fever associated with mutations in the gene MEFV encoding pyrin. It is expressed mainly in neutrophils and macrophages, and modulates the production of the potent pro-inflammatory cytokine interleukin-1 beta through regulation of nuclear factor-kappa B and caspase-1. The MEFV gene expression depends on multiple levels of regulation. Sequence variants located in the promoter and at the 3'-untranslated region of the gene modulate this expression. Two studies demonstrated decreased mRNA levels in FMF patients compared with healthy subjects, whereas two others found no significant differences. The diverse experimental settings may have resulted in variable quantification of the 15 splice variants that have been identified recently. Some of these isoforms are regulated by nonsense-mediated decay in both cell-and transcript-specific manner, and may be differentially translated in THP1 cells. In addition, pyrin may be cleaved by caspase 1. The full-length pyrin was less abundant than the cleaved fragment in mononuclear cells from FMF patients than in controls, whereas the opposite was observed in granulocytes. Altogether, the regulation of MEFV expression is more complex than anticipated in both physiological and pathological conditions. Its deregulation is likely to alter the inflammasome function and subsequently result in uncontrolled inflammation as seen in FMF. Genes and Immunity (2011) 12, 497-503; doi:10.1038/gene.2011.53; published online 21 July 201

Results and complications of anterior chamber intraocular lenses

Catedra Oftalmologie USMF “Nicolae Testemiţanu”The puprose of the study is to make a comparison in the evolution of anterior chamber intraocular lens (AC-IOL) per primam and per secundam. 120 patients have been operated in the Ophthalmology University Clinic in Chisinau during 2000-2009. Early and late postoperative complications were most frequent in the patients with AC-IOLs per primam. Scopul lucrării este a analiza comparativ evoluţia cazurilor operate cu implant de cristalin artificial de cameră anterioară (IOL-CA) atât per primam cât şi per secundam. 120 pacienţi au fost operaţi în Clinica Oftalmologică Universitară din Chişinău în perioada 2000-2009. Complicaţiile postoperatorii imediate şi tardive au fost cele mai frecvente la pacienţii cu IOL-CA per primam