Pea-barley intercrop N dynamics in farmers fields

Knowledge about crop performances in farmers’ fields provides a link between on-farm practice and re-search. Thereby scientists may improve their ability to understand and suggest solutions for the problems facing those who have the responsibility of making sound agricultural decisions. Nitrogen (N) availability is known to be highly heterogeneous in terrestrial plant communities (Stevenson and van Kessel, 1997), a heterogeneity that in natural systems is often associated with variation in the distri-bution of plant species. In intercropping systems the relative proportion of component crops is influenced by the distribution of growth factors such as N in both time and space (Jensen, 1996). In pea-barley intercrops, an increase in the N supply promotes the growth of barley thereby decreasing the N accumulation of pea and giving rise to changes in the relative proportions of the intercropped components (Jensen, 1996). The pres-sure of weeds may, however, significantly change the dynamics in intercrops (Hauggaard-Nielsen et al., 2001). Data from farmers’ fields may provide direct, spatially explicit information for evaluating the poten-tials of improving the utilisation of field variability by intercrops

A novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection

Background: Polymicrobial infections represent a great challenge for the clarification of disease etiology and the development of comprehensive diagnostic or therapeutic tools, particularly for fastidious and difficult-to-cultivate bacteria. Using bovine digital dermatitis (DD) as a disease model, we introduce a novel strategy to study the pathogenesis of complex infections. Results: The strategy combines meta-transcriptomics with high-density peptide-microarray technology to screen for in vivo-expressed microbial genes and the host antibody response at the site of infection. Bacterial expression patterns supported the assumption that treponemes were the major DD pathogens but also indicated the active involvement of other phyla (primarily Bacteroidetes). Bacterial genes involved in chemotaxis, flagellar synthesis and protection against oxidative and acidic stress were among the major factors defining the disease. Conclusions: The extraordinary diversity observed in bacterial expression, antigens and host antibody responses between individual cows pointed toward microbial variability as a hallmark of DD. Persistence of infection and DD reinfection in the same individual is common; thus, high microbial diversity may undermine the host's capacity to mount an efficient immune response and maintain immunological memory towards DD. The common antigenic markers identified here using a high-density peptide microarray address this issue and may be useful for future preventive measures against DD.Fil: Marcatili, Paolo. Technical University of Denmark; DinamarcaFil: Nielsen, Martin W.. Technical University of Denmark; DinamarcaFil: Sicheritz Ponten, Thomas. Technical University of Denmark; DinamarcaFil: Jensen, Tim K.. Technical University of Denmark; DinamarcaFil: Schafer Nielsen, Claus. Schafer-N ApS; DinamarcaFil: Boye, Mette. Hospital of Southern Jutland; DinamarcaFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Klitgaard, Kirstine. Technical University of Denmark; Dinamarc