Colonoscopy: Advanced and Emerging Techniques-A Review of Colonoscopic Approaches to Colorectal Conditions.

A complete colonoscopy is key in the diagnostic and therapeutic approaches to a variety of colorectal diseases. Major challenges are incomplete polyp removal and missed polyps, particularly in the setting of a difficult colonoscopy. There are a variety of both well-established and newer techniques that have been developed to optimize polyp detection, perform complete polypectomy, and endoscopically treat various complications and conditions such as strictures and perforations. The objective of this article is to familiarize the colorectal surgeon with techniques utilized by advanced endoscopists

Sex-associated differences in baseline urinary metabolites of healthy adults.

The biological basis for gender variability among disease states is not well established. There have been many prior efforts attempting to identify the unique urine metabolomic profiles associated with specific diseases. However, there has been little advancement in investigating the metabolomic differences associated with gender, which underlies the misconception that risk factors and treatment regimens should be the same for both male and female patients. This present study aimed to identify biologically-meaningful baseline sex-related differences using urine samples provided by healthy female and male participants. To elucidate whether urinary metabolic signatures are globally distinct between healthy males and females, we applied metabolomics profiling of primary metabolism with comprehensive bioinformatics analyses on urine samples from 60 healthy males and females. We found that levels of α-ketoglutarate and 4-hydroxybutyric acid increased 2.3-fold and 4.41-fold in males compared to females, respectively. Furthermore, chemical similarity enrichment analysis revealed that differentially expressed metabolites, such as saturated fatty acids, TCA, and butyrates, were significantly related to the gender effect. These findings indicate that there are baseline sex-related differences in urinary metabolism, which should be considered in biomarker discovery, diagnosis, and treatment of bladder diseases, such as interstitial cystitis

Variable Intrinsic Absorption in Mrk 279

We examine the variability in the intrinsic absorption in the Seyfert 1 galaxy Mrk 279 using three epochs of observations from the Far Ultraviolet Spectroscopic Explorer (FUSE) and two epochs of observations with the Space Telescope Imaging Spectrograph on the Hubble Space Telescope. Rather than finding simple photoionization responses of the absorbing gas to changes in the underlying continuum, the observed changes in the absorption profiles can be understood more clearly if the effective covering fraction of the gas in all emission components, continuum and broad and intermediate velocity width emission lines, is accounted for. While we do not uniquely solve for all of these separate covering fractions and the ionic column densities using the spectral data, we examine the parameter space using previously well-constrained solutions for continuum and single emission component covering fractions. Assuming full coverage of the continuum, we find that of the two velocity components of the Mrk 279 absorption most likely associated with its outflow, one likely has zero coverage of the intermediate line region while the other does not. For each component, however, the broad line region is more fully covered than the intermediate line region. Changes in the O VI column densities are unconstrained due to saturation, but we show that small changes in the nonsaturated C IV and N V column densities are consistent with the outflow gas having zero or partial covering of the intermediate line region and an ionization parameter changing from ~0.01 to ~0.1 from 2002 to 2003 as the UV continuum flux increased by a factor of ~8. The absence of a change in the C III absorbing column density is attributed to this species arising outside the Mrk 279 outflow.Comment: 36 pages, 18 figures, accepted to Ap

Recruitment, growth and mortality of an Antarctic hexactinellid sponge, Anoxycalyx joubini.

Polar ecosystems are sensitive to climate forcing, and we often lack baselines to evaluate changes. Here we report a nearly 50-year study in which a sudden shift in the population dynamics of an ecologically important, structure-forming hexactinellid sponge, Anoxycalyx joubini was observed. This is the largest Antarctic sponge, with individuals growing over two meters tall. In order to investigate life history characteristics of Antarctic marine invertebrates, artificial substrata were deployed at a number of sites in the southern portion of the Ross Sea between 1967 and 1975. Over a 22-year period, no growth or settlement was recorded for A. joubini on these substrata; however, in 2004 and 2010, A. joubini was observed to have settled and grown to large sizes on some but not all artificial substrata. This single settlement and growth event correlates with a region-wide shift in phytoplankton productivity driven by the calving of a massive iceberg. We also report almost complete mortality of large sponges followed over 40 years. Given our warming global climate, similar system-wide changes are expected in the future

Satellite microwave assessment of Northern Hemisphere lake ice phenology from 2002 to 2015

A new automated method enabling consistent satellite assessment of seasonal lake ice phenology at 5 km resolution was developed for all lake pixels (water coverage  ≥  90 %) in the Northern Hemisphere using 36.5 GHz H-polarized brightness temperature (Tb) observations from the Advanced Microwave Scanning Radiometer for EOS and Advanced Microwave Scanning Radiometer 2 (AMSR-E/2) sensors. The lake phenology metrics include seasonal timing and duration of annual ice cover. A moving t test (MTT) algorithm allows for automated lake ice retrievals with daily temporal fidelity and 5 km resolution gridding. The resulting ice phenology record shows strong agreement with available ground-based observations from the Global Lake and River Ice Phenology Database (95.4 % temporal agreement) and favorable correlations (R) with alternative ice phenology records from the Interactive Multisensor Snow and Ice Mapping System (R = 0.84 for water clear of ice (WCI) dates; R = 0.41 for complete freeze over (CFO) dates) and Canadian Ice Service (R = 0.86 for WCI dates; R = 0.69 for CFO dates). Analysis of the resulting 12-year (2002–2015) AMSR-E/2 ice record indicates increasingly shorter ice cover duration for 43 out of 71 (60.6 %) Northern Hemisphere lakes examined, with significant (p  \u3c  0.05) regional trends toward earlier ice melting for only five lakes. Higher-latitude lakes reveal more widespread and larger trends toward shorter ice cover duration than lower-latitude lakes, consistent with enhanced polar warming. This study documents a new satellite-based approach for rapid assessment and regional monitoring of seasonal ice cover changes over large lakes, with resulting accuracy suitable for global change studies

Dynamic stretching is effective as static stretching at increasing flexibility

This study examined the effect of dynamic and static (standard) stretching on hamstring flexibility. Twenty-five female volleyball players were randomly assigned to dynamic (n = 12) and standard (n = 13) stretching groups. The experimental group trained with repetitive dynamic stretching exercises, while the standard modality group trained with static stretching exercises. The stretching interventions were equivalent in the time at stretch and were performed three days a week for four weeks. Both stretching groups showed significant improvements (P < .001) in range of motion (ROM) during the intervention. However, no difference in gains in the range of motion between stretching groups was observed. It was concluded that both dynamic stretching and standard stretching are effective at increasing ROM

Intrinsic Absorption in the Spectrum of NGC 7469: Simultaneous Chandra, FUSE, and STIS Observations

We present simultaneous X-ray, far-ultraviolet, and near-ultraviolet spectra of the Seyfert 1 galaxy NGC 7469 obtained with the Chandra X-Ray Observatory, the Far Ultraviolet Spectroscopic Explorer, and the Space Telescope Imaging Spectrograph on the Hubble Space Telescope. Previous non-simultaneous observations of this galaxy found two distinct UV absorption components, at -560 and -1900 km/s, with the former as the likely counterpart of the X-ray absorber. We confirm these two absorption components in our new UV observations, in which we detect prominent O VI, Ly alpha, N V, and C IV absorption. In our Chandra spectrum we detect O VIII emission, but no significant O VIII or O VII absorption. We also detect a prominent Fe K alpha emission line in the Chandra spectrum, as well as absorption due to hydrogen-like and helium-like neon, magnesium, and silicon at velocities consistent with the -560 km/s UV absorber. The FUSE and STIS data reveal that the H I and C IV column densities in this UV- and X-ray- absorbing component have increased over time, as the UV continuum flux decreased. We use measured H I, N V, C IV, and O VI column densities to model the photoionization state of both absorbers self-consistently. We confirm the general physical picture of the outflow in which the low velocity component is a highly ionized, high density absorber with a total column density of 10^20 cm^-2, located near the broad emission line region, although due to measurable columns of N V and C IV, we assign it a somewhat smaller ionization parameter than found previously, U~1. The high velocity UV component is of lower density, log N=18.6, and likely resides farther from the central engine as we find its ionization parameter to be U=0.08.Comment: Minor correction to abstract; STScI eprint #1683; 50 pages, incl. 19 figures, 4 tables; Accepted to Ap

Conditional degradation of SDE2 by the Arg/N-End rule pathway regulates stress response at replication forks

Multiple pathways counteract DNA replication stress to prevent genomic instability and tumorigenesis. The recently identified human SDE2 is a genome surveillance protein regulated by PCNA, a DNA clamp and processivity factor at replication forks. Here, we show that SDE2 cleavage after its ubiquitin-like domain generates Lys-SDE2^(Ct), the C-terminal SDE2 fragment bearing an N-terminal Lys residue. Lys-SDE2^(Ct) constitutes a short-lived physiological substrate of the Arg/N-end rule proteolytic pathway, in which UBR1 and UBR2 ubiquitin ligases mediate the degradation. The Arg/N-end rule and VCP/p97^(UFD1-NPL4) segregase cooperate to promote phosphorylation-dependent, chromatin-associated Lys-SDE2^(Ct) degradation upon UVC damage. Conversely, cells expressing the degradation-refractory K78V mutant, Val-SDE2^(Ct), fail to induce RPA phosphorylation and single-stranded DNA formation, leading to defects in PCNA-dependent DNA damage bypass and stalled fork recovery. Together, our study elucidates a previously unappreciated axis connecting the Arg/N-end rule and the p97-mediated proteolysis with the replication stress response, working together to preserve replication fork integrity