Promoting Healthy Habits and Active Living for Children and Families at the Well-Child Visit

The 2019 Rutland Regional Medical Center Community Health Needs Assessment reported that 16% of adolescents and 30% of adults in Rutland County, Vermont are obese. This exceeds the average numbers for the state. Primary care practitioners in Rutland have struggled to find evidence-based, efficient programs to help address this issue. The 5-2-1-0 Program is an evidence-driven, low cost, and easy to remember intervention that assesses, prevents, and manages childhood obesity by promoting healthy habits and active living for children and their families. Using Brandon Medical Center, a Community Health Center of the Rutland Region, as the pilot site, this project aimed to implement the 5-2-1-0 Healthy Habits Questionnaire at all well-child visits and to propose a strategy for further integration of the 5-2-1-0 message into surrounding community health centers.https://scholarworks.uvm.edu/fmclerk/1538/thumbnail.jp

High-Resolution Analysis of Cytosine Methylation in Ancient DNA

Epigenetic changes to gene expression can result in heritable phenotypic characteristics that are not encoded in the DNA itself, but rather by biochemical modifications to the DNA or associated chromatin proteins. Interposed between genes and environment, these epigenetic modifications can be influenced by environmental factors to affect phenotype for multiple generations. This raises the possibility that epigenetic states provide a substrate for natural selection, with the potential to participate in the rapid adaptation of species to changes in environment. Any direct test of this hypothesis would require the ability to measure epigenetic states over evolutionary timescales. Here we describe the first single-base resolution of cytosine methylation patterns in an ancient mammalian genome, by bisulphite allelic sequencing of loci from late Pleistocene Bison priscus remains. Retrotransposons and the differentially methylated regions of imprinted loci displayed methylation patterns identical to those derived from fresh bovine tissue, indicating that methylation patterns are preserved in the ancient DNA. Our findings establish the biochemical stability of methylated cytosines over extensive time frames, and provide the first direct evidence that cytosine methylation patterns are retained in DNA from ancient specimens. The ability to resolve cytosine methylation in ancient DNA provides a powerful means to study the role of epigenetics in evolution

Human Inborn Errors of Immunity: 2019 Update on the Classification from the International Union of Immunological Societies Expert Committee.

We report the updated classification of Inborn Errors of Immunity/Primary Immunodeficiencies, compiled by the International Union of Immunological Societies Expert Committee. This report documents the key clinical and laboratory features of 430 inborn errors of immunity, including 64 gene defects that have either been discovered in the past 2 years since the previous update (published January 2018) or were characterized earlier but have since been confirmed or expanded upon in subsequent studies. The application of next-generation sequencing continues to expedite the rapid identification of novel gene defects, rare or common; broaden the immunological and clinical phenotypes of conditions arising from known gene defects and even known variants; and implement gene-specific therapies. These advances are contributing to greater understanding of the molecular, cellular, and immunological mechanisms of disease, thereby enhancing immunological knowledge while improving the management of patients and their families. This report serves as a valuable resource for the molecular diagnosis of individuals with heritable immunological disorders and also for the scientific dissection of cellular and molecular mechanisms underlying inborn errors of immunity and related human diseases

Human Inborn Errors of Immunity: 2019 Update of the IUIS Phenotypical Classification.

Since 2013, the International Union of Immunological Societies (IUIS) expert committee (EC) on Inborn Errors of Immunity (IEI) has published an updated phenotypic classification of IEI, which accompanies and complements their genotypic classification into ten tables. This phenotypic classification is user-friendly and serves as a resource for clinicians at the bedside. There are now 430 single-gene IEI underlying phenotypes as diverse as infection, malignancy, allergy, autoimmunity, and autoinflammation. We herein report the 2019 phenotypic classification, including the 65 new conditions. The diagnostic algorithms are based on clinical and laboratory phenotypes for each of the ten broad categories of IEI

Going with the floe: tracking CESM Large Ensemble sea ice in the Arctic provides context for ship-based observations

In recent decades, Arctic sea ice has shifted toward a younger, thinner, seasonal ice regime. Studying and understanding this &ldquo;new&rdquo; Arctic will be the focus of a year-long ship campaign beginning in autumn 2019. Lagrangian tracking of sea ice floes in the Community Earth System Model Large Ensemble (CESM-LE) during representative &ldquo;perennial&rdquo; and &ldquo;seasonal&rdquo; time periods allows for understanding of the conditions that a floe could experience throughout the calendar year. These model tracks, put into context a single year of observations, provide guidance on how observations can optimally shape model development, and how climate models could be used in future campaign planning. The modeled floe tracks show a range of possible trajectories, though a Transpolar Drift trajectory is most likely. There is also a small but emerging possibility of high-risk tracks, including possible melt of the floe before the end of a calendar year. We find that a Lagrangian approach is essential in order to correctly compare the seasonal cycle of sea ice conditions between point-based observations and a model. Because of high variability in the melt season sea ice conditions, we recommend in situ sampling over a large range of ice conditions for a more complete understanding of how ice type and surface conditions affect the observed processes. We find that sea ice predictability emerges rapidly during the autumn freeze-up and anticipate that process-based observations during this period may help elucidate the processes leading to this change in predictability. </div

Examination of the Internet Public Library as dynamic, collaborative teaching laboratory

The Internet Public Library (www.ipl.org) is a collaborative online learning environment that involves LIS faculty and students as well as working librarians around the world. Over the past twelve years, the IPL has become more collaborative with the introduction of new technologies and cultural changes. The IPL team consists of a variety of people from around the world: LIS faculty, IPL staff librarians, masters and doctoral students, working librarians, and technology specialists. Through the IPL, library and information professionals are being prepared to meet the challenges and opportunities of the digital environment: the dynamic nature of information technology, changing social and cultural norms, and an abundance of freely available content are presented in the digital reference environment. Extensive training material has been prepared by the IPL staff and feedback is provided to students by IPL staff, faculty and volunteers. In addition to the learning environment, the IPL also offers a test bed for research related to collaborative online learning environments. For examples, questions such as the role of Web 2.0 resources such as Wikipedia, Facebook, MySpace and other social computing sites will be explored

Nonlinear gyrokinetic simulations of the I-mode high confinement regime and comparisons with experimenta)

For the first time, nonlinear gyrokinetic simulations of I-mode plasmas are performed and compared with experiment. I-mode is a high confinement regime, featuring energy confinement similar to H-mode, but without enhanced particle and impurity particle confinement [D. G. Whyte et al., Nucl. Fusion 50, 105005 (2010)]. As a consequence of the separation between heat and particle transport, I-mode exhibits several favorable characteristics compared to H-mode. The nonlinear gyrokinetic code GYRO [J. Candy and R. E. Waltz, J Comput. Phys. 186, 545 (2003)] is used to explore the effects of E × B shear and profile stiffness in I-mode and compare with L-mode. The nonlinear GYRO simulations show that I-mode core ion temperature and electron temperature profiles are more stiff than L-mode core plasmas. Scans of the input E × B shear in GYRO simulations show that E × B shearing of turbulence is a stronger effect in the core of I-mode than L-mode. The nonlinear simulations match the observed reductions in long wavelength density fluctuation levels across the L-I transition but underestimate the reduction of long wavelength electron temperature fluctuation levels. The comparisons between experiment and gyrokinetic simulations for I-mode suggest that increased E × B shearing of turbulence combined with increased profile stiffness are responsible for the reductions in core turbulence observed in the experiment, and that I-mode resembles H-mode plasmas more than L-mode plasmas with regards to marginal stability and temperature profile stiffness.United States. Department of Energy (Contract No. DE-FC02-99ER54512-CMOD)United States. Department of Energy. Office of Science (Contract No. DE-AC02- 05CH11231

The Ever-Increasing Array of Novel Inborn Errors of Immunity : an Interim Update by the IUIS Committee

The most recent updated classification of inborn errors of immunity/primary immunodeficiencies, compiled by the International Union of Immunological Societies Expert Committee, was published in January 2020. Within days of completing this report, it was already out of date, evidenced by the frequent publication of genetic variants proposed to cause novel inborn errors of immunity. As the next formal report from the IUIS Expert Committee will not be published until 2022, we felt it important to provide the community with a brief update of recent contributions to the field of inborn errors of immunity. Herein, we highlight studies that have identified 26 additional monogenic gene defects that reach the threshold to represent novel causes of immune defects.Peer reviewe

Human Inborn Errors of Immunity : 2022 Update on the Classification from the International Union of Immunological Societies Expert Committee

We report the updated classification of inborn errors of immunity, compiled by the International Union of Immunological Societies Expert Committee. This report documents the key clinical and laboratory features of 55 novel monogenic gene defects, and 1 phenocopy due to autoantibodies, that have either been discovered since the previous update (published January 2020) or were characterized earlier but have since been confirmed or expanded in subsequent studies. While variants in additional genes associated with immune diseases have been reported in the literature, this update includes only those that the committee assessed that reached the necessary threshold to represent novel inborn errors of immunity. There are now a total of 485 inborn errors of immunity. These advances in discovering the genetic causes of human immune diseases continue to significantly further our understanding of molecular, cellular, and immunological mechanisms of disease pathogenesis, thereby simultaneously enhancing immunological knowledge and improving patient diagnosis and management. This report is designed to serve as a resource for immunologists and geneticists pursuing the molecular diagnosis of individuals with heritable immunological disorders and for the scientific dissection of cellular and molecular mechanisms underlying monogenic and related human immune diseases.Peer reviewe

The (im)materiality of literacy : the significance of subjectivity to new literacies research.

This article deconstructs the online and offline experience to show its complexities and idiosyncratic nature. It proposes a theoretical framework designed to conceptualise aspects of meaning-making across on- and offline contexts. In arguing for the ‘(im)materiality’ of literacy, it makes four propositions which highlight the complex and diverse relationships between the immaterial and material associated with meaning-making. Complementing existing sociocultural perspectives on literacy, the article draws attention to the significance of relationships between space, mediation, materiality and embodiment to literacy practices. This in turn emphasises the importance of the subjective in understanding how different locations, experiences and so forth inflect literacy practice. The article concludes by drawing on the Deleuzian concept of the ‘baroque’ to suggest that this focus on articulations between the material and immaterial helps us to see literacy as multiply and flexibly situated