The challenge of career progression and work-life balance in consulting business in Germany

This thesis examines how individuals define and understand work-life balance and career progression in the consulting business sector in Germany. Since this client-focused work environment requires travel and long working weeks, employees face challenges that impact on their personal life and health. The thesis explores, through a series of qualitative interviews, how the research participants attempt to address and cope with work demands and how they seek to achieve a better balance between work and non-work activities (such as children, household, care of parents, hobbies) whilst being able progress in their chosen career. The wider context of this paper is a triangle of consulting business, career progression and work-life balance. Demographic changes; competition for skilled workers, which is referred to as the ‘war for talent’, impact organisational success in a competitive business. Organisations need to respond to these changes through work-life practices and a supportive organisational culture if they are to attract and retain professionals. Based on 28 telephone interviews, the data indicates differences in employee perceptions of worklife balance and career progression. While time flexibility and work-life practices are often provided and named as key to managing work and non-work activities, participants describe an unspoken expectation to be present and work longer hours in order to progress up the career ladder. Participants propose ideas that could allow them to progress their career in balance with other non-work responsibilities. For this challenge, personal responsibility plays a central role. This thesis (Doctor of Business Administration) is valuable for employees to reflect on their own work-life balance and career and to adapt the ideas of participants to manage existing challenges. The results can equally be used by organisations to reframe work-life practices and culture to support their employees in better managing their work and non-work lives to retain and attract talent in a competitive business environment

Dataset from a microphone array measurement of a rotating line source

The following instruction gives a summary of an microphone array measurement conducted at the TU Berlin in March 2021. The setup consists of a rotating line source and a sunflower array with 63 microphones as well as a laser trigger. The rotating line source is made of 8 Visaton BF37 speakers on a 800 mm rod. The given data set contains 8 measurement files with different source characteristics and rotational speeds

The Vertebrate Genome Annotation (Vega) database

The Vertebrate Genome Annotation (Vega) database (http://vega.sanger.ac.uk) has been designed to be a community resource for browsing manual annotation of finished sequences from a variety of vertebrate genomes. Its core database is based on an Ensembl-style schema, extended to incorporate curation-specific metadata. In collaboration with the genome sequencing centres, Vega attempts to present consistent high-quality annotation of the published human chromosome sequences. In addition, it is also possible to view various finished regions from other vertebrates, including mouse and zebrafish. Vega displays only manually annotated gene structures built using transcriptional evidence, which can be examined in the browser. Attempts have been made to standardize the annotation procedure across each vertebrate genome, which should aid comparative analysis of orthologues across the different finished regions

The Origins, Evolution, and Functional Potential of Alternative Splicing in Vertebrates

Alternative splicing (AS) has the potential to greatly expand the functional repertoire of mammalian transcriptomes. However, few variant transcripts have been characterized functionally, making it difficult to assess the contribution of AS to the generation of phenotypic complexity and to study the evolution of splicing patterns. We have compared the AS of 309 protein-coding genes in the human ENCODE pilot regions against their mouse orthologs in unprecedented detail, utilizing traditional transcriptomic and RNAseq data. The conservation status of every transcript has been investigated, and each functionally categorized as coding (separated into coding sequence [CDS] or nonsense-mediated decay [NMD] linked) or noncoding. In total, 36.7% of human and 19.3% of mouse coding transcripts are species specific, and we observe a 3.6 times excess of human NMD transcripts compared with mouse; in contrast to previous studies, the majority of species-specific AS is unlinked to transposable elements. We observe one conserved CDS variant and one conserved NMD variant per 2.3 and 11.4 genes, respectively. Subsequently, we identify and characterize equivalent AS patterns for 22.9% of these CDS or NMD-linked events in nonmammalian vertebrate genomes, and our data indicate that functional NMD-linked AS is more widespread and ancient than previously thought. Furthermore, although we observe an association between conserved AS and elevated sequence conservation, as previously reported, we emphasize that 30% of conserved AS exons display sequence conservation below the average score for constitutive exons. In conclusion, we demonstrate the value of detailed comparative annotation in generating a comprehensive set of AS transcripts, increasing our understanding of AS evolution in vertebrates. Our data supports a model whereby the acquisition of functional AS has occurred throughout vertebrate evolution and is considered alongside amino acid change as a key mechanism in gene evolution

Ensembl 2005

The Ensembl (http://www.ensembl.org/) project provides a comprehensive and integrated source of annotation of large genome sequences. Over the last year the number of genomes available from the Ensembl site has increased by 7 to 16, with the addition of the six vertebrate genomes of chimpanzee, dog, cow, chicken, tetraodon and frog and the insect genome of honeybee. The majority have been annotated automatically using the Ensembl gene build system, showing its flexibility to reliably annotate a wide variety of genomes. With the increased number of vertebrate genomes, the comparative analysis provided to users has been greatly improved, with new website interfaces allowing annotation of different genomes to be directly compared. The Ensembl software system is being increasingly widely reused in different projects showing the benefits of a completely open approach to software development and distribution

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

An Extension of the Virtual Rotating Array Method Using Arbitrary Microphone Configurations for the Localization of Rotating Sound Sources

The characterization of rotating aeroacoustic sources using microphone array methods has been proven to be a useful tool. One technique to identify rotating sources is the virtual rotating array method. The method interpolates the pressure time data signals between the microphones in a stationary array to compensate the motion of the rotating sources. One major drawback of the method is the requirement of ring array geometries that are centred around the rotating axis. This contribution extends the virtual rotating array method to arbitrary microphone configurations. Two different ways to interpolate the time signals between the microphone locations are proposed. The first method constructs a mesh between the microphone positions using Delaunay-triangulation and interpolates over the mesh faces using piecewise linear functions. The second one is a meshless technique which is based on radial basis function interpolation. The methods are tested on synthetic array data from a benchmark test case as well as on experimental data obtained with a spiral array and a five-bladed fan

Optimal heating system assessment

katedra elektroenergetik