Advanced natural laminar flow airfoil with high lift to drag ratio

An experimental verification of a high performance natural laminar flow (NLF) airfoil for low speed and high Reynolds number applications was completed in the Langley Low Turbulence Pressure Tunnel (LTPT). Theoretical development allowed for the achievement of 0.70 chord laminar flow on both surfaces by the use of accelerated flow as long as tunnel turbulence did not cause upstream movement of transition with increasing chord Reynolds number. With such a rearward pressure recovery, a concave type deceleration was implemented. Two-dimensional theoretical analysis indicated that a minimum profile drag coefficient of 0.0026 was possible with the desired laminar flow at the design condition. With the three-foot chord two-dimensional model constructed for the LTPT experiment, a minimum profile drag coefficient of 0.0027 was measured at c sub l = 0.41 and Re sub c = 10 x 10 to the 6th power. The low drag bucket was shifted over a considerably large c sub l range by the use of the 12.5 percent chord trailing edge flap. A two-dimensional lift to drag ratio (L/D) was 245. Surprisingly high c sub l max values were obtained for an airfoil of this type. A 0.20 chort split flap with 60 deg deflection was also implemented to verify the airfoil's lift capabilities. A maximum lift coefficient of 2.70 was attained at Reynolds numbers of 3 and 6 million

Methane Emission in a Specific Riparian-Zone Sediment Decreased with Bioelectrochemical Manipulation and Corresponded to the Microbial Community Dynamics

Dissimilatory metal-reducing bacteria are widespread in terrestrial ecosystems, especially in anaerobic soils and sediments. Thermodynamically, dissimilatory metal reduction is more favorable than sulfate reduction and methanogenesis but less favorable than denitrification and aerobic respiration. It is critical to understand the complex relationships, including the absence or presence of terminal electron acceptors, that govern microbial competition and coexistence in anaerobic soils and sediments, because subsurface microbial processes can effect greenhouse gas emissions from soils, possibly resulting in impacts at the global scale. Here, we elucidated the effect of an inexhaustible, ferrous-iron and humic-substance mimicking terminal electron acceptor by deploying potentiostatically poised electrodes in the sediment of a very specific stream riparian zone in Upstate New York state. At two sites within the same stream riparian zone during the course of six weeks in the spring of 2013, we measured CH4 and N2/N2O emissions from soil chambers containing either poised or unpoised electrodes, and we harvested biofilms from the electrodes to quantify microbial community dynamics. At the upstream site, which had a lower vegetation cover and highest soil temperatures, the poised electrodes inhibited CH4 emissions by ~45% (when normalized to remove temporal effects). CH4 emissions were not significantly impacted at the downstream site. N2/N2O emissions were generally low at both sites and were not impacted by poised electrodes. We did not find a direct link between bioelectrochemical treatment and microbial community membership; however, we did find a correspondence between environment/function and microbial community dynamics

Evaluation and Development of Blackbird Repellents for Agricultural Applications

We evaluated several registered fungicides and insecticides, and several natural compounds as candidate blackbird repellents for protecting agricultural production. We tested more than 750 red-winged blackbirds at the National Wildlife Research Center’s outdoor animal research facility in Fort Collins, CO to evaluate (1) their preference for treated versus untreated rice and sunflower seeds and (2) their consumption of seeds treated with varying concentrations of candidate repellents. Concentrations were varied between 10% and 200% of labeled application rates. With few exceptions, blackbirds discriminated between untreated seeds and seeds treated with one of the candidate repellents. We observed greatest repellency with caffeine + sodium benzoate, GWN-4770, Lorsban, and Tilt. Additional lab efficacy tests are planned for anthraquinone seed treatments, Flock Buster repellent, and Cobalt insecticide. Field residue and efficacy tests are also needed to evaluate and develop promising foliar repellents for protection of ripening crops

Evaluation and Development of Blackbird Repellents for Agricultural Applications

We evaluated several registered fungicides and insecticides, and several natural compounds as candidate blackbird repellents for protecting agricultural production. We tested more than 750 red-winged blackbirds at the National Wildlife Research Center’s outdoor animal research facility in Fort Collins, CO to evaluate (1) their preference for treated versus untreated rice and sunflower seeds and (2) their consumption of seeds treated with varying concentrations of candidate repellents. Concentrations were varied between 10% and 200% of labeled application rates. With few exceptions, blackbirds discriminated between untreated seeds and seeds treated with one of the candidate repellents. We observed greatest repellency with caffeine + sodium benzoate, GWN-4770, Lorsban, and Tilt. Additional lab efficacy tests are planned for anthraquinone seed treatments, Flock Buster repellent, and Cobalt insecticide. Field residue and efficacy tests are also needed to evaluate and develop promising foliar repellents for protection of ripening crops

Variations in D/H and D/O from New FUSE Observations

We use data obtained with the Far Ultraviolet Spectroscopic Explorer (FUSE) to determine the interstellar abundances of DI, NI, OI, FeII, and H2 along the sigh tlines to WD1034+001, BD+393226, and TD132709. Our main focus is on determining the D/H, N/H, O/H, and D/O ratios along these sightlines, with log N(H) > 20.0, that probe gas well outside of the Local Bubble. Hubble Space Telescope (HST) and International Ultraviolet Explorer (IUE) archival data are used to determine the HI column densities along the WD1034+001 and TD132709 sightlines, respectively. For BD+393226, a previously published N(HI) is used. We find (D/H)x10^5 = 2.14 + 0.53 - 0.45, 1.17 + 0.31 - 0.25, and 1.86 + 0.53 - 0.43, and (D/O)x10^2 = 6.31 + 1.79 - 1.38, 5.62 + 1.61 - 1.31, and 7.59 + 2.17 - 1.76, for the WD1034+001, BD+393226, and TD132709 sightlines, respectively (all 1 si gma). The scatter in these three D/H ratios exemplifies the scatter that has been found by other authors for sightlines with column densities in the range 19.2 < log N(H) < 20.7. The D/H ratio toward WD1034+001 and all the D/O ratios derived here are inconsistent with the Local Bubble value and are some of the highest in the literature. We discuss the implications of our measurements for the determination of the present-epoch abundance of deuterium, and for the different scenarios that try to explain the D/H variations. We present a study of D/H as a function of the average sightline gas density, using the ratios derived in this work as well as ratios from the literature, which suggests that D/H decreases with increasing gas volume density. Similar behaviors by other elements such Fe and Si have been interpreted as the result of depletion into dust grains.Comment: Accepted for publication in the Ap

Order out of Randomness : Self-Organization Processes in Astrophysics

Self-organization is a property of dissipative nonlinear processes that are governed by an internal driver and a positive feedback mechanism, which creates regular geometric and/or temporal patterns and decreases the entropy, in contrast to random processes. Here we investigate for the first time a comprehensive number of 16 self-organization processes that operate in planetary physics, solar physics, stellar physics, galactic physics, and cosmology. Self-organizing systems create spontaneous {\sl order out of chaos}, during the evolution from an initially disordered system to an ordered stationary system, via quasi-periodic limit-cycle dynamics, harmonic mechanical resonances, or gyromagnetic resonances. The internal driver can be gravity, rotation, thermal pressure, or acceleration of nonthermal particles, while the positive feedback mechanism is often an instability, such as the magneto-rotational instability, the Rayleigh-B\'enard convection instability, turbulence, vortex attraction, magnetic reconnection, plasma condensation, or loss-cone instability. Physical models of astrophysical self-organization processes involve hydrodynamic, MHD, and N-body formulations of Lotka-Volterra equation systems.Comment: 61 pages, 38 Figure

Evaluation of Bird Shield as a blackbird repellent in ripening rice and sunflower fields

Chemical repellents sometimes can provide a nonlethal alternative for reducing wildlife impacts to agricultural production. In late summer and autumn 2002, we evaluated Bird Shield™ (active ingredient: methyl anthranilate, Bird Shield Repellent Corporation, Spokane, Wash.) as a blackbird (Icteridae) repellent in Missouri rice fields and North Dakota sunflower fields. We selected 5 pairs of ripening rice fields in southeastern Missouri and randomly allocated treatments (treated and control) within pairs. The repellent was aerially applied by fixed-winged aircraft at the recommended label rate and volume (1.17 L Bird Shield/ha and 46.7 L/ha, respectively); 1 field received 2X the label rate. We observed no difference in average bird activity (birds/minute) between treated and control fields over the 3-day posttreatment period (P = 0.503). We used reversed-phase liquid chromatography to quantify methyl anthranilate residues in treated fields. The maximum concentration of methyl anthranilate in rice samples was 4.71 μg/g. This concentration was below reported threshold values that irritate birds. In North Dakota we selected 6 pairs of sunflower fields used by foraging blackbirds. We randomly selected 1 field from each pair for 2 aerial applications of Bird Shield at the label-recommended rate ~1 week apart. The remaining 6 fields served as controls. Daily bird counts, starting the first day of application and continuing for 5–7 days after the second application, showed similar numbers of blackbirds within treated and control fields (P = 0.964). We observed no difference in sunflower damage within treated and control fields (P = 0.172) prior and subsequent to the treatment. Bird Shield was not effective for repelling blackbirds from ripening rice and sunflower fields

Evaluation of Bird Shield as a blackbird repellent in ripening rice and sunflower fields

Chemical repellents sometimes can provide a nonlethal alternative for reducing wildlife impacts to agricultural production. In late summer and autumn 2002, we evaluated Bird Shield™ (active ingredient: methyl anthranilate, Bird Shield Repellent Corporation, Spokane, Wash.) as a blackbird (Icteridae) repellent in Missouri rice fields and North Dakota sunflower fields. We selected 5 pairs of ripening rice fields in southeastern Missouri and randomly allocated treatments (treated and control) within pairs. The repellent was aerially applied by fixed-winged aircraft at the recommended label rate and volume (1.17 L Bird Shield/ha and 46.7 L/ha, respectively); 1 field received 2X the label rate. We observed no difference in average bird activity (birds/minute) between treated and control fields over the 3-day posttreatment period (P = 0.503). We used reversed-phase liquid chromatography to quantify methyl anthranilate residues in treated fields. The maximum concentration of methyl anthranilate in rice samples was 4.71 μg/g. This concentration was below reported threshold values that irritate birds. In North Dakota we selected 6 pairs of sunflower fields used by foraging blackbirds. We randomly selected 1 field from each pair for 2 aerial applications of Bird Shield at the label-recommended rate ~1 week apart. The remaining 6 fields served as controls. Daily bird counts, starting the first day of application and continuing for 5–7 days after the second application, showed similar numbers of blackbirds within treated and control fields (P = 0.964). We observed no difference in sunflower damage within treated and control fields (P = 0.172) prior and subsequent to the treatment. Bird Shield was not effective for repelling blackbirds from ripening rice and sunflower fields

Novel Rhizosphere Soil Alleles for the Enzyme 1-Aminocyclopropane-1-Carboxylate Deaminase Queried for Function with an In Vivo Competition Assay

ABSTRACT Metagenomes derived from environmental microbiota encode a vast diversity of protein homologs. How this diversity impacts protein function can be explored through selection assays aimed to optimize function. While artificially generated gene sequence pools are typically used in selection assays, their usage may be limited because of technical or ethical reasons. Here, we investigate an alternative strategy, the use of soil microbial DNA as a starting point. We demonstrate this approach by optimizing the function of a widely occurring soil bacterial enzyme, 1-aminocyclopropane-1-carboxylate (ACC) deaminase. We identified a specific ACC deaminase domain region (ACCD-DR) that, when PCR amplified from the soil, produced a variant pool that we could swap into functional plasmids carrying ACC deaminase-encoding genes. Functional clones of ACC deaminase were selected for in a competition assay based on their capacity to provide nitrogen to Escherichia coli in vitro . The most successful ACCD-DR variants were identified after multiple rounds of selection by sequence analysis. We observed that previously identified essential active-site residues were fixed in the original unselected library and that additional residues went to fixation after selection. We identified a divergent essential residue whose presence hints at the possible use of alternative substrates and a cluster of neutral residues that did not influence ACCD performance. Using an artificial ACCD-DR variant library generated by DNA oligomer synthesis, we validated the same fixation patterns. Our study demonstrates that soil metagenomes are useful starting pools of protein-coding-gene diversity that can be utilized for protein optimization and functional characterization when synthetic libraries are not appropriate