31 research outputs found
A brief introduction to the model microswimmer {\it Chlamydomonas reinhardtii}
The unicellular biflagellate green alga {\it Chlamydomonas reinhardtii} has
been an important model system in biology for decades, and in recent years it
has started to attract growing attention also within the biophysics community.
Here we provide a concise review of some of the aspects of {\it Chlamydomonas}
biology and biophysics most immediately relevant to physicists that might be
interested in starting to work with this versatile microorganism.Comment: 16 pages, 7 figures. To be published as part of EPJ S
Universal entrainment mechanism governs contact times with motile cells
Contact between particles and motile cells underpins a wide variety of
biological processes, from nutrient capture and ligand binding, to grazing,
viral infection and cell-cell communication. The window of opportunity for
these interactions is ultimately determined by the physical mechanism that
enables proximity and governs the contact time. Jeanneret et al. (Nat. Comm. 7:
12518, 2016) reported recently that for the biflagellate microalga
Chlamydomonas reinhardtii contact with microparticles is controlled by events
in which the object is entrained by the swimmer over large distances. However,
neither the universality of this interaction mechanism nor its physical origins
are currently understood. Here we show that particle entrainment is indeed a
generic feature for microorganisms either pushed or pulled by flagella. By
combining experiments, simulations and analytical modelling we reveal that
entrainment length, and therefore contact time, can be understood within the
framework of Taylor dispersion as a competition between advection by the no
slip surface of the cell body and microparticle diffusion. The existence of an
optimal tracer size is predicted theoretically, and observed experimentally for
C. reinhardtii. Spatial organisation of flagella, swimming speed, swimmer and
tracer size influence entrainment features and provide different trade-offs
that may be tuned to optimise microbial interactions like predation and
infection.Comment: New analytical entrainment theory; includes Supplementary
informations as Appendix; Supplementary movies available upon reques
Hamiltonian traffic dynamics in microfluidic-loop networks
Recent microfluidic experiments revealed that large particles advected in a
fluidic loop display long-range hydrodynamic interactions. However, the
consequences of such couplings on the traffic dynamics in more complex networks
remain poorly understood. In this letter, we focus on the transport of a finite
number of particles in one-dimensional loop networks. By combining numerical,
theoretical, and experimental efforts, we evidence that this collective process
offers a unique example of Hamiltonian dynamics for hydrodynamically
interacting particles. In addition, we show that the asymptotic trajectories
are necessarily reciprocal despite the microscopic traffic rules explicitly
break the time reversal symmetry. We exploit these two remarkable properties to
account for the salient features of the effective three-particle interaction
induced by the exploration of fluidic loops
Emergent hyperuniformity in periodically-driven emulsions
We report the emergence of large-scale hyperuniformity in microfluidic
emulsions. Upon periodic driving confined emulsions undergo a first-order
transition from a reversible to an irreversible dynamics. We evidence that this
dynamical transition is accompanied by structural changes at all scales
yielding macroscopic yet finite hyperuniform structures. Numerical simulations
are performed to single out the very ingredients responsible for the
suppression of density fluctuations. We show that as opposed to equilibrium
systems the long-range nature of the hydrodynamic interactions are not required
for the formation of hyperuniform patterns, thereby suggesting a robust
relation between reversibility and hyperuniformity which should hold in a broad
class of periodically driven materials.Comment: 5p, 3f, submitte
Confinement-induced accumulation and de-mixing of microscopic active-passive mixtures
Understanding the out-of-equilibrium properties of noisy microscale systems and the extent to which they can be modulated externally, is a crucial scientific and technological challenge. It holds the promise to unlock disruptive new technologies ranging from targeted delivery of chemicals within the body to directed assembly of new materials. Here we focus on how active matter can be harnessed to transport passive microscopic systems in a statistically predictable way. Using a minimal active-passive system of weakly Brownian particles and swimming microalgae, we show that spatial confinement leads to a complex non-monotonic steady-state distribution of colloids, with a pronounced peak at the boundary. The particles’ emergent active dynamics is well captured by a space-dependent Poisson process resulting from the space-dependent motion of the algae. Based on our findings, we then realise experimentally the de-mixing of the active-passive suspension, opening the way for manipulating colloidal objects via controlled activity fields
Confinement-induced accumulation and de-mixing of microscopic active-passive mixtures
Understanding the out-of-equilibrium properties of noisy microscale systems and the extent to which they can be modulated externally, is a crucial scientific and technological challenge. It holds the promise to unlock disruptive new technologies ranging from targeted delivery of chemicals within the body to directed assembly of new materials. Here we focus on how active matter can be harnessed to transport passive microscopic systems in a statistically predictable way. Using a minimal active-passive system of weakly Brownian particles and swimming microalgae, we show that spatial confinement leads to a complex non-monotonic steady-state distribution of colloids, with a pronounced peak at the boundary. The particles’ emergent active dynamics is well captured by a space-dependent Poisson process resulting from the space-dependent motion of the algae. Based on our findings, we then realise experimentally the de-mixing of the active-passive suspension, opening the way for manipulating colloidal objects via controlled activity fields
Confinement enhances the diversity of microbial flow fields
Despite their importance in many biological, ecological and physical
processes, microorganismal fluid flows under tight confinement have not been
investigated experimentally. Strong screening of Stokelets in this geometry
suggests that the flow fields of different microorganisms should be universally
dominated by the 2D source dipole from the swimmer's finite-size body.
Confinement therefore is poised to collapse differences across microorganisms,
that are instead well-established in bulk. Here we combine experiments and
theoretical modelling to show that, in general, this is not correct. Our
results demonstrate that potentially minute details like microswimmers'
spinning and the physical arrangement of the propulsion appendages have in fact
a leading role in setting qualitative topological properties of the
hydrodynamic flow fields of micro-swimmers under confinement. This is well
captured by an effective 2D model, even under relatively weak confinement.
These results imply that active confined hydrodynamics is much richer than in
bulk, and depends in a subtle manner on size, shape and propulsion mechanisms
of the active components.Comment: Accepted for publication in Physical Review Letters. 5 pages and 4
figures, plus Supplementary Materia
The Reasons for Discrepancies in TargetVolume Delineation: A SASRO Study on Head-and-Neck and Prostate Cancers
Purpose: : To understand the reasons for differences in the delineation of target volumes between physicians. Material and Methods: : 18 Swiss radiooncology centers were invited to delineate volumes for one prostate and one head-and-neck case. In addition, a questionnaire was sent to evaluate the differences in the volume definition (GTV [gross tumor volume], CTV [clinical target volume], PTV [planning target volume]), the various estimated margins, and the nodes at risk. Coherence between drawn and stated margins by centers was calculated. The questionnaire also included a nonspecific series of questions regarding planning methods in each institution. Results: : Fairly large differences in the drawn volumes were seen between the centers in both cases and also in the definition of volumes. Correlation between drawn and stated margins was fair in the prostate case and poor in the head-and-neck case. The questionnaire revealed important differences in the planning methods between centers. Conclusion: : These large differences could be explained by (1) a variable knowledge/interpretation of ICRU definitions, (2) variable interpretations of the potential microscopic extent, (3) difficulties in GTV identification, (4) differences in the concept, and (5) incoherence between theory (i.e., stated margins) and practice (i.e., drawn margins
Geometrically protected reversibility in hydrodynamic Loschmidt-echo experiments
[eng] When periodically driven, a number of markedly different systems (colloids, droplets, grains, flux lines) have revealed a transition from a reversible to an irreversible dynamics that hardly depends on the very nature of the interacting objects. Yet, no clear structural signature has been found for this collective self-organization. Here, we demonstrate an archetypal Loschmidt-echo experiment involving thousands of droplets that interact in a reversible fashion via a viscous fluid. First, we show that periodically driven microfluidic emulsions self-organize and geometrically protect their macroscopic reversibility. Self-organization is not merely dynamical: it has a clear structural signature. Second, we show that, above a maximal shaking amplitude, structural order and reversibility are lost simultaneously through a first-order non-equilibrium phase transition. We account for this discontinuous transition in terms of a memory-loss process. Finally, we suggest potential applications of microfluidic echo as a robust tool to tailor colloidal self-assembly at large scales