Inestabilidades y deformación en la cubierta sedimentaria de la parte superior del talud del margen continental de Aquitania, norte del cañon del Capbreton (Golfo de Vizcaya)

Acoustic and core data have recently been collected on the shelf break and the upper part of the slope of the south Aquitaine continental margin. They reveal the major role played by mass-flow gravity processes in deposit erosion and redistribution, modelling of the sea-bed, and transfer of sediment toward the deep-sea. The study region is bounded in the south by the Capbreton canyon. The northern area, which shows a smooth morphology, is characterised by small-scale deformations due to sediment creep or low-amplitude slide processes. The deformations are associated with mini listric-like faults that bound packets of sediments in which the deposit geometry is typical of constructional sediment waves. These sediment waves result from the interaction of depositional and gravity deformation processes. In the southern area, closer to the canyon, wave-like structures are still present but mostly of smaller size. They only result from gravity deformation processes without any evidence of constructional processes. In the vicinity of the Capbreton canyon, the shelf break and upper slope have a much more uneven morphology with sedimentary reliefs, escarpments and depressions directed toward the canyon thalweg. The depressions look like slide scars, and could be the result of regressive slides initiated at the top of the canyon flank. The age of the sliding event responsible for the formation of the depression observed today could be middle to upper Quaternary. Since their formation, these depressions act as conduits that channel the transfer of shelf sediment into the canyon, as demonstrated by the occurrence of a meandering channel on the sea-floor of one depression.Se recogieron datos acústicos y de testigos (cores) en el límite de la plataforma y la parte superior del talud en el margen continental del sur de Aquitania. Estos datos revelan el importante papel que juegan los procesos de flujos en masa por gravedad en la erosión y redistribución de los depósitos, el modelado del lecho marino y la transferencia de sedimentos hacia el fondo del océano. La región de estudio está limitada al sur por el cañón de Capbreton. El área norte, que presenta una morfología suave, está caracterizada por deformaciones de pequeña escala debidas al arrastre de sedimentos o procesos de deslizamiento de baja amplitud. Las deformaciones están asociadas a mini fallas de tipo lístrico que delimitan bolsas de sedimentos en las cuales la geometría del depósito es típica de ondas de sedimento constructivas. Estas ondas sedimentarias resultan de la interacción de los procesos de deformación deposicionales y por gravedad. En el área sur, cerca del cañón, están todavía presentes estructuras similares a ondas, pero la mayor parte son de pequeño tamaño. Estas estructuras son el resultado de los procesos de deformación por gravedad sin ninguna evidencia de procesos constructivos. En las proximidades del cañón de Capbreton, el margen continental y el talud superior tienen una morfología mucho más desigual con relieves sedimentarios, escarpaduras y depresiones dirigidas hacia el talweg del cañón. Las depresiones semejan marcas de deslizamientos y pueden ser el resultado de deslizamientos regresivos iniciados en la parte superior de los flancos del cañón. Los eventos de deslizamiento responsables de la formación de las depresiones observadas actualmente podrían datarse en el Cuaternario medio o superior. Desde su formación, estas depresiones funcionan como conductos que canalizan la transferencia de sedimentos de plataforma hacia el cañón, tal y como demuestra la presencia de un canal con meandros en el fondo de una de estas depresiones

T Cell Reactivity against Mycolyl Transferase Antigen 85 of M. tuberculosis in HIV-TB Coinfected Subjects and in AIDS Patients Suffering from Tuberculosis and Nontuberculous Mycobacterial Infections

The mycolyl transferase antigen 85 complex is a major secreted protein family from mycobacterial culture filtrate, demonstrating powerful T cell stimulatory properties in most HIV-negative, tuberculin-positive volunteers with latent M.tuberculosis infection and only weak responses in HIV-negative tuberculosis patients. Here, we have analyzed T cell reactivity against PPD and Ag85 in HIV-infected individuals, without or with clinical symptoms of tuberculosis, and in AIDS patients with disease caused by nontuberculous mycobacteria. Whereas responses to PPD were not significantly different in HIV-negative and HIV-positive tuberculin-positive volunteers, responses to Ag85 were significantly decreased in the HIV-positive (CDC-A and CDC-B) group. Tuberculosis patients demonstrated low T cell reactivity against Ag85, irrespective of HIV infection, and finally AIDS patients suffering from NTM infections were completely nonreactive to Ag85. A one-year follow-up of twelve HIV-positive tuberculin-positive individuals indicated a decreased reactivity against Ag85 in patients developing clinical tuberculosis, highlighting the protective potential of this antigen

Pathogen and Circadian Controlled 1 (PCC1) regulates polar lipid content, ABA-related responses, and pathogen defence in Arabidopsis thaliana

Pathogen and Circadian Controlled 1 (PCC1) was previously characterized as a regulator of defence against pathogens and stress-activated transition to flowering. Plants expressing an RNA interference construct for the PCC1 gene (iPCC1 plants) showed a pleiotropic phenotype. They were hypersensitive to abscisic acid (ABA) as shown by reduced germination potential and seedling establishment, as well as reduced stomatal aperture and main root length in ABA-supplemented media. In addition, iPCC1 plants displayed alterations in polar lipid contents and their corresponding fatty acids. Importantly, a significant reduction in the content of phosphatidylinositol (PI) was observed in iPCC1 leaves when compared with wild-type plants. A trend in reduced levels of 18:0 and increased levels of 18:2 and particularly 18:3 was also detected in several classes of polar lipids. The enhanced ABA-mediated responses and the reduced content of PI might be responsible for iPCC1 plants displaying a complex pattern of defence against pathogens of different lifestyles. iPCC1 plants were more susceptible to the hemi-biotrophic oomycete pathogen Phytophthora brassicae and more resistant to the necrotrophic fungal pathogen Botrytis cinerea compared with wild-type plant

Randomized trial of calcipotriol combined with 5-fluorouracil for skin cancer precursor immunotherapy

BACKGROUND. Actinic keratosis is a precursor to cutaneous squamous cell carcinoma. Long treatment durations and severe side effects have limited the efficacy of current actinic keratosis treatments. Thymic stromal lymphopoietin (TSLP) is an epithelium-derived cytokine that induces a robust antitumor immunity in barrier-defective skin. Here, we investigated the efficacy of calcipotriol, a topical TSLP inducer, in combination with 5-fluorouracil (5-FU) as an immunotherapy for actinic keratosis. METHODS. The mechanism of calcipotriol action against skin carcinogenesis was examined in genetically engineered mouse models. The efficacy and safety of 0.005% calcipotriol ointment combined with 5% 5-FU cream were compared with Vaseline plus 5-FU for the field treatment of actinic keratosis in a randomized, double-blind clinical trial involving 131 participants. The assigned treatment was self-applied to the entirety of the qualified anatomical sites (face, scalp, and upper extremities) twice daily for 4 consecutive days. The percentage of reduction in the number of actinic keratoses (primary outcome), local skin reactions, and immune activation parameters were assessed. RESULTS. Calcipotriol suppressed skin cancer development in mice in a TSLP-dependent manner. Four-day application of calcipotriol plus 5-FU versus Vaseline plus 5-FU led to an 87.8% versus 26.3% mean reduction in the number of actinic keratoses in participants (P < 0.0001). Importantly, calcipotriol plus 5-FU treatment induced TSLP, HLA class II, and natural killer cell group 2D (NKG2D) ligand expression in the lesional keratinocytes associated with a marked CD4(+) T cell infiltration, which peaked on days 10–11 after treatment, without pain, crusting, or ulceration. CONCLUSION. Our findings demonstrate the synergistic effects of calcipotriol and 5-FU treatment in optimally activating a CD4(+) T cell–mediated immunity against actinic keratoses and, potentially, cancers of the skin and other organs. TRIAL REGISTRATION. ClinicalTrials.gov NCT02019355. FUNDING. Not applicable (investigator-initiated clinical trial)

Service availability and readiness assessment of maternal, newborn and child health services at public health facilities in Madagascar

The Service Availability and Readiness Assessment (SARA) survey was adapted and used to generate information on service availability and the readiness of maternal, newborn and child health facilities to provide basic health care interventions for obstetric care, neonatal and child health in Madagascar. The survey collected data from fifty-two public health facilities, ranging from university hospitals (CHU), referral district and regional hospitals (CHD/ CHRR) to basic health centres (CSB). For basic emergency obstetric and newborn care (BEmONC) readiness, on average, CHU had nine (71.8%), CHD/CHRR had eight and CSB had six out of the thirteen tracer items. Regarding the availability of the eleven tracer items for comprehensive CEmONC services, on average a CHU had nine ( 80.0%), a CHRR had eight (71.1%) and a CHD that is the only type of hospitals in rural area had three tracer items (30.0%). Tracer item availability results are low, indicating the need to strengthen supplies at basic health centers in order to improve the chances of success of Madagascar’s Roadmap for accelerating the reduction of the maternal and neonatal mortality 2015-2019, and meeting Sustainable Development Goals 3.1 and 3.Keywords: Madagascar, Maternal and Child health services, Service availability and readiness assessment, Public health facilitie

The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

AsES (Acremonium strictum Elicitor and Subtilisin) is a novel extracellular elicitor protein produced by the avirulent isolate SS71 of the opportunist strawberry fungal pathogen A. strictum. Here we describe the activity of AsES in the plant-pathogen system Arabidopsis thaliana–Botrytis cinerea. We show that AsES renders A. thaliana plants resistant to the necrotrophic pathogen B. cinerea, both locally and systemically and the defense response observed is dose-dependent. Systemic, but not local resistance is dependent on the length of exposure to AsES. The germination of the spores in vitro was not inhibited by AsES, implying that protection to B. cinerea is due to the induction of the plant defenses. These results were further supported by the findings that AsES differentially affects mutants impaired in the response to salicylic acid, jasmonic acid and ethylene, suggesting that AsES triggers the defense response through these three signaling pathways