Ayurvedic management of Ducchen’s Muscular Dystrophy - A Case report

Ducchen’s muscular dystrophy is most common X-linked recessive disorder affecting 30 in 100,000 live male births. The primary cause of this disease is mutations in Dystrophin gene which is essential for the structural and functional integrity of muscle. It is a progressive muscle wasting disease in which patients frequently develop contractures and lose the ability to walk between 6 and 12 years of age. With progressive disease most patients succumb to death from respiratory failure and cardiac dysfunction in their twenties. As this is a genetic disorder we can consider it as Adibala Pravritta Vyadhi. As Mamsa Kshaya is seen at some muscles and Mamsa Vriddhi at other this is an Avarana Vata Vyadhi. In both Upsthambha and Nirupasthmbha Vatavyadhi, Basthi is considered as prime choice of treatment. A Variety of Ksheerabasti in the form of Kalabasti is studied in this condition by taking subjective and objective parameters. As this has given better improvement with no adverse effects in the patient, it can be tried in large number of patients

Studies on Alum as Milk Coagulating Agent in the Manufacture of Paneer – An Indian Cottage Cheese

Alum is a white crystalline double sulphate of aluminum and potassium that is commonly used as an astringent. Utilising its protein binding property, alum was studied as coagulating agent of milk in the preparation of paneer. Paneer is a soft variety of cottage cheese very popular in Indian sub-continent, used in various culinary dishes. Its   preparation essentially involves acid coagulation of hot milk and pressing of the hot coagulum to form a compact block which is then cooled by immersing in chilled water. Citric acid and vinegar are commonly used as coagulating agents of milk during paneer manufacture. However, when alum was used as a coagulating agent, it was observed that most of the proteins were amalgamated during coagulation process resulting in enhanced yield of paneer. It also resulted in reduced solids losses in whey. However, use of alum resulted in a slight astringency in paneer, hence it was recommended to be used in combination with common coagulant i.e. citric acid. The solids content (losses) in whey were 6.62, 6.08, and 6.32 %, respectively for citric acid, alum and citric acid + alum mixture used as coagulating agents. Similarly, the yields of paneer were 16.42, 19.04 and 16.99 %. The alum coagulated paneer looked glossy and attractive which was indicated by reflectance values of 83.75% as against 79% for control paneer sample. The paneer made by alum coagulation had slightly brittle texture

Preclinical evaluation of Boswellia serrata for anxiolytic activity

Background: Boswellia serrata (BS)has been described in the ancient Ayurvedic texts Sushruta Samhita and Charaka Samhita. It possesses anti-inflammatory, analgesic, anti-arthritic and antioxidant properties. It is found that BShelps in surging of GABA levels in mice brain. The aim of this study was to evaluate the possible anxiolytic activity of BSin Swiss albino mice by light and dark arena (LDA) and elevated plus maze (EPM) models.Methods: In this study, BS(50 mg/kg, 100 mg/kg and 200 mg/kg; p.o) was evaluated for anxiolytic action and compared with standard drug (diazepam) and control (normal saline) in mice by LDA and EPM models. In LDA, number of entries and time spent in light and dark boxes were noted for individual mouse. Similarly, number of entries and time spent in open and closed arms were recorded for EPM model.Results: One-way Analysis of Variance (ANOVA) followed by Dunnett’s post-hoc test was used to analyze the data. BSin a dose of 50 mg/kg has shown significant increase in time spent in light box (p<0.05) and decrease in time spent in dark box (p<0.05) when compared to control group in LDA model. Similarly, in EPM model 200 mg/kg of BS significantly increased time spent in open arm (p<0.001) and decrease in time spent in closed arm (p<0.001) when compared to control group.Conclusion: BS in dose of 50 mg/kg and 200 mg/kg has significant anxiolytic action in animal models

Dedifferentiation of committed epithelial cells into stem cells in vivo

Summary Cellular plasticity contributes to the regenerative capacity of plants, invertebrates, teleost fishes, and amphibians. In vertebrates, differentiated cells are known to revert into replicating progenitors, but these cells do not persist as stable stem cells. We now present evidence that differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. Following the ablation of airway stem cells, we observed a surprising increase in the proliferation of committed secretory cells. Subsequent lineage tracing demonstrated that the luminal secretory cells had dedifferentiated into basal stem cells. Dedifferentiated cells were morphologically indistinguishable from stem cells and they functioned as well as their endogenous counterparts to repair epithelial injury. Indeed, single secretory cells clonally dedifferentiated into multipotent stem cells when they were cultured ex vivo without basal stem cells. In contrast, direct contact with a single basal stem cell was sufficient to prevent secretory cell dedifferentiation. In analogy to classical descriptions of amphibian nuclear reprogramming, the propensity of committed cells to dedifferentiate was inversely correlated to their state of maturity. This capacity of committed cells to dedifferentiate into stem cells may play a more general role in the regeneration of many tissues and in multiple disease states, notably cancer

Impact of Virtual Interprofessional COVID-19 disaster simulation Tabletop Exercise (VICTEr) workshop on Disaster Preparedness among Interprofessional trainees in a tertiary care teaching hospital in India

INTRODUCTION: Disaster planning is of significant importance for the healthcare professional and the healthcare setting. Hospital-based disaster protocols form the cornerstone of disaster response. There is a paucity of data on disaster preparedness training using the virtual tabletop exercise (VTTX) module for interprofessional education from in-hospital and prehospital settings. With the coronavirus disease 2019 (COVID-19) pandemic, we have seen a paradigm shift of education strategies to the virtual realm. Here we attempt to study the impact of an online tabletop exercise workshop on the knowledge and confidence of disaster preparedness among Interprofessional trainees. MATERIAL AND METHODS: Interprofessional trainees from medical, dental, nursing, respiratory therapy, and paramedic domains who consented were included in this study. Institutional ethics committee approval was received and the study was registered with the clinical trials registry India (CTRI), before initiation. The VTTX module has been adapted from the World Health Organization (WHO) COVID-19 training resources. Three international experts from the disaster medicine domain validated the module, questionnaire, and feedback. Wilcoxon signed-rank test was used to compare the parameters (Knowledge and confidence level) pre and post-workshop. RESULTS: A total of 76 candidates with a mean age was 21.67 ± 2.5 (range:19–36) were part of the workshop. Comparison of the median scores and interquartile range of confidence level and knowledge respectively before [38 (29.25–45.75), 9 (7–11)] and after [51.50 (45–60), 11 (10–12)] the workshop showed vital significance (p-value &lt; 0.001). All participants gave positive feedback on the workshop meeting the objectives. The majority agreed that the workshop improved their self-preparedness (90%) and felt that the online platform was appropriate (97.5%). CONCLUSIONS: This study sheds light on the positive impact of the online VTTX based workshop on disaster preparedness training among interprofessional trainees. Disaster preparedness training using available online platforms may be effectively executed with the VICTEr workshop even during the COVID-19 pandemic. The VICTEr workshop serves as a primer for developing online modules for effective pandemic preparedness training in interprofessional education

An integrated cell atlas of the lung in health and disease

Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number of donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations of individual studies and capture the variability present in the population. Here we present the integrated Human Lung Cell Atlas (HLCA), combining 49 datasets of the human respiratory system into a single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents a consensus cell type re-annotation with matching marker genes, including annotations of rare and previously undescribed cell types. Leveraging the number and diversity of individuals in the HLCA, we identify gene modules that are associated with demographic covariates such as age, sex and body mass index, as well as gene modules changing expression along the proximal-to-distal axis of the bronchial tree. Mapping new data to the HLCA enables rapid data annotation and interpretation. Using the HLCA as a reference for the study of disease, we identify shared cell states across multiple lung diseases, including SPP1+ profibrotic monocyte-derived macrophages in COVID-19, pulmonary fibrosis and lung carcinoma. Overall, the HLCA serves as an example for the development and use of large-scale, cross-dataset organ atlases within the Human Cell Atlas

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

<i style="mso-bidi-font-style:normal">Khoa jalebi</i>, a unique traditional product of Central India

96-102Khoa jalebi is a popular sweet consumed in central parts of India. It is one of the foods consumed during fasting days by Hindus and Muslim community. In order to characterise the typical attributes of this traditional product, khoa jalebi samples were collected from Nagpur, Nasik, Indore, Raipur and Wardha cities and evaluated for sensory, physico-chemical and textural characteristics. The study showed that khoa jalebi resembled traditional maida jalebi but possessed thicker coils heavier in nature. The colour was light brown to dark brown and the texture was soft yet chewy. The number of coils was restricted to a maximum of four and sugar syrup remained embedded within the coils. The composition of the product varied widely the moisture, fat, protein, lactose, sucrose and ash contents being 18.21 – 28.69, 12.33 – 16.77, 2.78 – 6.98, 7.39 – 13.13, 15.20 – 48.07 and 0.60 – 1.29%, respectively. Variations were also observed in ingredients used for the preparation of <i style="mso-bidi-font-style: normal">khoa jalebi and the method adopted for manufacture varied from halwai to <i style="mso-bidi-font-style: normal">halwai. Hence, there is a need for characterization and process optimization for the preparation of khoa jalebi so that dairy industry can produce this <i style="mso-bidi-font-style: normal">khoa based sweet on large scale

<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Development of technology for bamboo seeds <i style="mso-bidi-font-style: normal">payasam- </i>a traditional product of tribals in Wayanad district of Kerala</span>

200-212<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">The bamboo [Bambusa bambos (L.) Voss] seeds payasam is a light brown to dark brown coloured product, the dark colour is due to dark jaggery used in its preparation. The payasam has flowable consistency and possesses pleasant caramel taste with a typical bamboo seeds flavor along with a dash of coconut flavor. Various ingredients of bamboo seeds payasam, viz. amount of bamboo seeds, water, jaggery, fresh coconut extract and milk along with process for preparation were optimized. The standardized method of preparation involved cleaning and washing of bamboo seeds, soaking 100 g seeds in excess of water for 6 h at ambient temperature, draining the water, addition of 300 mL of fresh water and pressure cooking at 15 psi for 10 min. The cooked grains were then mixed with jaggery syrup, 125 g of fresh coconut extract and 125 g toned milk. Then the entire mixture was heated and desiccated to a final weight of about 1 kg. The bamboo seeds payasam had a composition of total solids 38.75 %, fat 7.8 %, protein 4.96 %, lactose 0.56 %, sucrose 20.25 %, ash 0.84 % and other carbohydrates 4.34 %. Based on the sensory evaluation and textural results, it was observed that the shelf life of the payasam was 1-2 days at 30 oC and more than 15 days at 5 oC.</span

Development of technology for bamboo seeds payasam- a traditional product of tribals in Wayanad district of Kerala

The bamboo [Bambusa bambos (L.) Voss] seeds payasam is a light brown to dark brown coloured product, the dark colour is due to dark jaggery used in its preparation. The payasam has flowable consistency and possesses pleasant caramel taste with a typical bamboo seeds flavor along with a dash of coconut flavor. Various ingredients of bamboo seeds payasam, viz. amount of bamboo seeds, water, jaggery, fresh coconut extract and milk along with process for preparation were optimized. The standardized method of preparation involved cleaning and washing of bamboo seeds, soaking 100 g seeds in excess of water for 6 h at ambient temperature, draining the water, addition of 300 mL of fresh water and pressure cooking at 15 psi for 10 min. The cooked grains were then mixed with jaggery syrup, 125 g of fresh coconut extract and 125 g toned milk. Then the entire mixture was heated and desiccated to a final weight of about 1 kg. The bamboo seeds payasam had a composition of total solids 38.75 %, fat 7.8 %, protein 4.96 %, lactose 0.56 %, sucrose 20.25 %, ash 0.84 % and other carbohydrates 4.34 %. Based on the sensory evaluation and textural results, it was observed that the shelf life of the payasam was 1-2 days at 30 oC and more than 15 days at 5 oC.