10 research outputs found
Subpicogram Per Milliliter Detection of Interleukins Using Silicon Photonic Microring Resonators and an Enzymatic Signal Enhancement Strategy
The
detection of biomolecules at ultralow (low to subpicogram per
milliliter) concentrations and within complex, clinically relevant
matrices is a formidable challenge that is complicated by limitations
imposed by the Langmuir binding isotherm and mass transport, for surface-based
affinity biosensors. Here we report the integration of an enzymatic
signal enhancement scheme onto a multiplexable silicon photonic microring
resonator detection platform. To demonstrate the analytical value
of this combination, we simultaneously quantitated levels of the interleukins
IL-2, IL-6, and IL-8 in undiluted cerebrospinal fluid in an assay
format that is multiplexable, relatively rapid (90 min), and features
a 3 order of magnitude dynamic range and a limit of detection ≤1
pg/mL. The modular nature of this assay and technology should lend
itself broadly amenable to different analyte classes, making it a
versatile tool for biomarker analysis in clinically relevant settings