66 research outputs found
Morphogenesis and anatomico-physiological characteristics of reproductive organs of walnut (Juglans regia L.)
periodu od 2010. do 2013. godine u ekoloÅ”kim uslovima Kraljeva prouÄavane su anatomske, morfoloÅ”ke i fizioloÅ”ke osobine reproduktivnih organa kod pet sorti oraha (Å ampion, Å ejnovo, Gajzenhajmski 139, Gajzenhajmski 251 i Elit) i jednog spontanog sejanca.
U radu je dat detaljan opis graÄe muÅ”kih i ženskih cvetova na anatomskom i morfoloÅ”kom nivou. Morfometrijska analiza resa, antera, polenovih zrna, ženskih cvetova, semenih zametaka i embrionovih kesica pokazala je da na neke morfometrijske parametre ne utiÄu znaÄajno faktori spoljaÅ”nje sredine i da se oni mogu uspeÅ”no koristiti za determinaciju sorti.
Procesi diferencijacije muÅ”kih i ženskih cvetova, ukljuÄujuÄi i procese formiranja muÅ”kog i ženskog gametofita, odvijali su se sukcesivno i istim redosledom kod svih ispitivanih genotipova i u svim godinama, a razlike po godinama i meÄu genotipovima odnosile su se prvenstveno na vreme poÄetka i dužinu trajanja pojedinih faza razvitka. U radu su definisane, detaljno opisane i fotodokumentovane karakteristiÄne faze razvojnih procesa, a njihova dinamika prikazana je grafiÄki za svaki genotip. Tip dihogamije ispoljio je znaÄajan uticaj na dinamiku razvoja generativnih pupoljaka i cvetova. IzmeÄu mikrofenofaza u razvitku muÅ”kih i ženskih cvetova i stadijuma u formiranju spora i gameta u njima utvrÄen je visok stepen podudarnosti, Å”to omoguÄava da se na osnovu morfoloÅ”kih promena na cvetovima prate histoloÅ”ke promene u sporogenim tkivima i gametofitima cvetova.
Na morfoloÅ”ke osobine rodnog drveta (debljinu i dužinu rodnih letorasta i plodonosnih prirasta, broj nodusa i strukturu pupoljaka na granÄicama i po nodusima) znaÄajan uticaj ispoljili su genotip i faktori spoljaÅ”nje sredine. Na indukciju muÅ”kih i ženskih cvasti znaÄajno su uticali genotip, godina, razvijenost granÄice i položaj pupoljka na granÄici. Prisustvo ženskih cvetova ili mladih plodova na plodonosnim
mladarima u nekim sluÄajevima imalo je negativan uticaj na indukciju novih generativnih pupoljaka na plodonosnim prirastima...Over the period of 2010-2013, under the environmental conditions of Kraljevo, the anatomical, morphological and physiological properties of reproductive organs were studied in five walnut cultivars ('Å ampion', 'Sheinovo', 'Geisenheim 139', 'Geisenheim 251' and 'Elit') and one chance seedling.
A detailed description of both the anatomical and the morphological structure of staminate and pistillate flowers is provided. The morphometric analysis of catkins, anthers, pollen grains, pistillate flowers, ovules and embryo sacs showed that some morphometric parameters are not significantly affected by environmental factors and that they can be successfully used for cultivar identification.
The differentiation of staminate and pistillate flowers, including the formation of male and female gametophytes, occurred in successive stages following the same sequence of events in all genotypes studied and in all years, with differences across years and genotypes primarily regarding the onset time and length of particular developmental stages. Characteristic stages in the developmental processes are defined, detailed and photodocumented, and their dynamics is graphically presented for each genotype. The type of dichogamy exhibited a significant effect on the developmental dynamics of generative buds and flowers. A high degree of correspondence was determined between the microphenological phases of staminate and pistillate flowers and the stages of spore and gamete formation, which enables the observation of histological changes in sporogenous tissues and gametophytes based on changes in flower morphology.
The morphological properties of the bearing wood (thickness and length of bearing shoots and bourse shoots, number of nodes, and structure of buds on the shoots and at nodes) were significantly affected by genotype and environmental factors. The induction of staminate and pistillate inflorescences was significantly affected by genotype, year, degree of shoot development and bud position on the shoot..
Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress
Background: The neurobiology underlying depression has not yet been fully identified, but is thought to result from molecular and cellular abnormalities that interact with genetic and environmental factors. Depression is twice as prevalent in women as in men, however, females remain underrepresented in preclinical research. In addition to the neurotransmission theory of depression, the inflammatory processes and the disrupted signaling pathways also play a crucial role in the pathophysiology of depression. The WKY rat strain has long been established as a model of depression. These rats demonstrate an exaggerated response to stress compared to other strains. WKY strain fail to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and considered to be nonresponsive to antidepressant drugs. The hippocampus and the medial prefrontal cortex (mPFC) are thought to be an important regions for depression. Brain-derived neurotrophic factor (BDNF) play a vital role in the pathophysiology of depression. BDNF-stimulated signaling cascades, including the phosphatidylinositol 3-kinase (PI3K)ā/serine threonine kinase (Akt), also implicated in depression and treatment respons. In the present study, we have examined the effects of CMS on behavior and BDNF and PI3K/Akt signaling in the hippocampus and mPFC of female WKY rats.
Method: In the experiment, we used three months old Wistar (WI) and WKY female rats. Animals were divided in two groups: control and animals exposed to CMS for 6 weeks. On the last day of stress procedure, animals were tested in elevated plus maze to determine the levels of anxiety. Animals were then sacrificed and hippocampus and mPFC were isolated. Levels of BDNF and pAkt were determined by Western blot method. Data were analyzed using the two way ANOVA and Tuckeyās post-hoc test.
Results: WKY rats showed significantly decreased number of rearings (by 70%, p<0.01),decreased number of total arm entries (by 21%, p<0.05) and the time spent in the open arms (by 73%, p<0.001) of the elevated plus-maze compared to WI control group. WKY females had a significantly lower level of BDNF in the hippocampus (by 12%, p<0.05) and mPFC (by 16%, p<0.05) and pAkt (by 14%, p<0.01) only in mPFC as compared to the WI female rats. Exposure of WKY females to CMS enhanced an anxiety-like behavior and hypolomotion (decrease in number of rearings by 31%, p<0.05, number of total arm entries by 89%, p<0.001, and timevspent in the open arms by 92%, p<0.001), further down-expression of BDNF in both brain areas (in PFC: by 15%, p<0.001; in hippocampus: by 7%, p<0.05) and Akt phosphorylation in the mPFC (by 17%, p<0.05) as well as a decreased pAkt in the hippocampus (by 36%, p<0.001).
Conclusions: The difference in the balance of BDNF and PI3K/Akt signaling pathways may be relevant to the resistance of WKY rats to antidepressant drug treatment and may be useful for developing new targets for depression treatment, especially in females.Abstracts of the 36th ECNP Congress 202
Quantification of radioisotopic pollution of soil from coal fired power plant surrounding
This paper is focused on determination of activity concentration of gamma emitters in surface layer of soil, in surrounding of the coal-fired power plant complex. Also, the impact of coal-fired power plant emissions on certain of physical and chemical properties of the soil was studied. The results of this study indicated that the operation of the power plant has no significant negative impact on the environment in terms of the content of radionuclides. The effect of the coal-fired power plant emissions on soil is a function of the pollutant gradient existing in the area. The increased soil acidity can adversely affect the microbiological and pedogenetic processes in soil which cause cation-anion imbalance and microbe population reduction to affect soil fertility. Ā© 2020, Editura Academiei Romane. All rights reserved
Celiac disease-specific and inflammatory bowel disease-related antibodies in patients with recurrent aphthous stomatitis
The etiology of recurrent aphthous stomatitis (RAS) remains unknown. RAS can be presented as primary, idiopathic condition and as a secondary RAS, which is associated with a systemic disease. The aim of our study was to evaluate the presence and concentrations of antibodies specific for celiac disease (CeD) and antibodies related to inflammatory bowel diseases (IBD) in patients with RAS without gastrointestinal symptoms. Antibodies against tissue transglutaminase (anti-tTG), deaminated gliadin peptides (DGP), deaminated gliadinanalogous fragments (anti-GAF-3X) and Saccharomyces cerevisiae (ASCA) were determined by ELISA and anti-neutrophil cytoplasmic antibodies (ANCA) by indirect immunoflurescence (IIF) in 57 patients with RAS and 60 control subjects. The prevalence of CeD specific antibodies did not differ between RAS patients and controls. However, the concentrations of IgA anti-tTG, IgA anti-GAF-3X antibodies in patients with RAS were significantly higher compared to controls (p = 0.002 and p = 0.04 respectively). Histological changes consistent with CeD were confirmed by duodenal biopsy in one RAS patient with highly positive IgA anti-tTG, anti-GAF-3X and anti-DGP antibodies. Higher prevalence along with higher concentrations of IgG ASCA were found in RAS patients compared to controls (p lt 0.01). Patients with positive IgG ASCA in the absence of clinical symptoms decided not to pursue any further testing. Dysfunction of oral mucosa and the exposure to various antigens might be a reason for the loss of tolerance resulting in increased production of autoantibodies. It seems likely that antibodies are markers of aberrant immune response, rather than key effectors involved in the pathogenesis of the disease
Uticaj hroniÄnog blagog stresa na ponaÅ”anje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije
Brojni dokazi ukazuju da socijalna izolacija može imati dugoroÄne efekte na ponaÅ”anje i u odgovoru na stres. Wistar-Kyoto (WK) soj pacova se smatra dobrim animalnim modelom rezistentne depresije jer su, za razliku od Wistar (W) soja, nakon izlaganja stresu neosetljivi na terapiju antidepresivima. U ovoj studiji smo izlagali ženke WK i W soja pacova hroniÄnom blagom stresu (CMS) u trajanju od 6 nedelja. Kontrolne životinje su bile u grupi po 3 jedinke, dok je CMS grupa pacova bila izolovana 3 nedelje pre poÄetka CMS procedure do kraja eksperimenta. Znaci anhedonije su tokom 6 nedelja bili procenjivani testom unosa saharoze, dok je stepen anksioznosti utvrÄen testom izdignutog plus lavirinta na kraju eksperimenta. Kontrolna WK grupa je pokazivala od prve do poslednje nedelje eksperimenta smanjen unos saharoze, hipolokomociju i smanjen broj ulaza u otvorene krake u odnosu na kontrolnu W grupu, Å”to potvrÄuje da ovaj soj i u odsustvu stresa pokazuje simptome depresije i anksioznosti. Izlaganje CMS je dovelo do dodatnih razlika izmeÄu sojeva u unosu saharoze. Izolacija u trajanju od 3 nedelje pre CMS dovela je kod oba soja do poveÄanja unosa saharoze, Å”to može ukazati na poveÄanu potrebu za zadovoljstvima. HroniÄni blagi stres je samo kod W soja doveo do znakova anhedonije, dok su ženke WK soja i nakon 6 nedelja CMS imale poveÄan unos saharoze. Ovi rezultati su relevantni za razumevanje naÄina na koji socijalna izolacija doprinosi osetljivosti pojedinca na poremeÄaje povezane sa stresom tokom života.TreÄi kongres biologa Srbije; Septembar 21-25, Zlatibor, 2022
Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression
Stress is a major precipitant of depression, the hippocampus (HPC) and prefrontal cortex (PFC) is the main structures affected by depressive disorders. Women are twice more likely to experience depression than men. The WKY rat strain has long been established as a model of depression. Initially bred from the Wistar (WI) rat as the control strain for the spontaneously hypertensive rat, WKY rats demonstrate an exaggerated stress response compared to other strains. WKY strain fails to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and is considered to be nonresponsive to antidepressant drugs. MAPK signaling pathway was most closely related to depression and antidepressant treatment. In the present study, we have examined the effects of CMS on behavior and p38 MAPK signaling in the hippocampus and the medial prefrontal cortex (mPFC) of female WKY rats. We used two very different behavioral tests: forced swim test (FST) and open field test (OFT). WKY unstressed controls exhibited increased immobility duration in the forced swim test and decreased activity in the open-field test compared to unstressed WI rats, while CMS did not further influence behavior. WKY showed increased expression of the p38 only in HPC and further exposure of WKY rats to CMS induces sustained-activation of p38 MAPK in this brain area. The present study demonstrated the brain region-specific protein signatures in the female WKY model with endogenous depression, providing novel insights into the pathogenesis of depression.Poster Session: Brain Metabolism & Dietary Intervention
Radionuclides and heavy metals in soil around the coal-fired power plant "Kostolac"
Ovaj rad je fokusiran na odreÄivanje koncentracije aktivnosti radionuklida (210Pb, 238U, 235U, 226Ra, 232Th i 40K) i koncentracije teÅ”kih metala (Pb, Mn i Zn ) u povrÅ”inskom sloju zemljiÅ”ta okoline termoelektrane "Kostolac A". Tehnike koje su koriÅ”Äene za analizu pomenutih radionuklida i teÅ”kih metala su gamaspektometrija i atomska apsorpciona spektrometrija, redom. Srednje vrednosti koncentracija aktivnosti ispitanih radionuklida, izražene u Bq/kg, iznosile su: 76,7 za 210Pb; 67,2 za 238U; 4,1 za 235U; 29 za 226Ra; 50,1 za 232Th i 611 za 40K. Srednje vrednosti koncentracija teÅ”kih metala, izražene u mg/kg, iznosile su: 28 za Pb; 775,4 za Mn i 105,5 za Zn. Rezultati ove studije su ukazali da rad termoelektrane nema znaÄajan negativan uticaj na okolinu u pogledu sadržaja radionuklida i teÅ”kih metala.This study is focused on determination of the
activity concentration of radionuclides (210Pb, 238U,
235U, 226Ra, 232Th i 40K) and concentration of heavy
metals (Pb, Mn i Zn) in surface layer of soil around
coal-fired power plant "Kostolac A". The techniques
used to analyze of mentioned radionuclides and heavy
metals are gamma spectometry and atomic absorption
spectrometry, respectively. The mean values of activity
concentrations of investigated radionuclides,
expressed as Bq/kg, were: 76.7 for 210Pb, 67.2 for 238U,
4.1 for 235U, 29 for 226Ra, 50.1 for 232Th and 611 for
40K. The mean concentrations of heavy metals,
expressed as mg/kg, were 28 for Pb, 775.4 for Mn and
105.5 for Zn. The obtained results indicated that
operation of the coal fired power plant has no
significant negative impact on the surrounding
environment with regard to the content of investigated
radionuclides and heavy metals12. Savetovanje Održivi razvoj BraniÄevskog okruga i energetskog kompleksa Kostolac : Maj 31., Kostolac, 2018
Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponaŔanjem
Istraživanja iz oblasti depresivnih poremeÄaja su sve viÅ”e usmerena ka hipotezi da su oksidativni stres i inflamatorni procesi takoÄe ukljuÄeni u patogenezu depresije. Signalni put koji ukljuÄuje janus kinazu 2 (JAK2) i pretvaraÄ signala i aktivator transkripcije 3 (STAT3) je povezan sa inflamacijom, a komponente ovog puta se eksprimiraju u razliÄitim regionima mozga, posebno u hipokampusu. Cilj ovog rada bio je da se ispita uloga elemenata JAK2/STAT3 signalnog puta kod depresije izazvane hroniÄnim blagim stresom (CMS). U radu su koriÅ”Äene ženke Wistar (W) i Wistar-Kyoto (WK) soja pacova. WK soj pokazuje neurondokrine promene, kao i promene u ponaÅ”anju sliÄne depresiji, zbog Äega se smatraju dobrim animalnim modelom endogene depresije. Odrasle W i WK ženke bile su izložene CMS u trajanju od 6 nedelja. Nakon ovog perioda jedinke su žrtvovane i izolovani su hipokampusi. Kvantitativna Western blot analiza naÅ”ih rezultata je pokazala da je kod kontrolne WK grupe, u odnosu na kontrolnu W grupu, fosforilacija JAK2 i STAT3 u hipokampusu poveÄana, Å”to ukazuje da je disregulacija ovog signalnog puta jedan od moguÄih uzroÄnika simptoma depresije kod ovog soja. HroniÄni stres je dodatno poveÄao ekspresiju fosforilisane forme ovih proteina kod oba soja. Ova studija pružila je nove dokaze o uÄeÅ”Äu JAK2/STAT3 signalnog puta u patogenezi depresije i može nas usmeriti u nalaženju novih, efikasnijih terapija u leÄenju rezistentne depresije.TreÄi kongres biologa Srbije; Septembar 21-25, Zlatibor, 2022
Medicinska nutritivna terapija gluten senzitivne enteropatije
Gluten sensitive enteropathy or celiac disease is clearly more prevalent than was previously thought. Gluten-sensitive enteropathy is the inherited autoimmune condition characterized by inflammation of the small intestine in response to the ingestion of gluten present in cereals such as wheat, barley, and rye. Elimination of the gluten from the diet assigned as medical nutrition therapy for gluten sensitive enteropathy is the first line of the treatment for coeliac disease. Gluten-free diet is an effective treatment that can alleviate symptoms and allows reduction in small intestinal inflammation and permits mucosal healing with improved absorptive function. However, gluten free diet is very complex and claimed. Also, strict adherence and life-long maintenance to the diet is very important in the aim to prevent macronutrient and micronutrient deficiency. Except that, regular involvement of the gluten free diet is important for preventing some complications such as anemia, osteoporosis, development of a neoplasm, and the risk of additional autoimmune disorders. Although there are many attempts for developing alternatives to the gluten-free diet, it currently still remains the only effective treatment option for gluten sensitive enteropathy.Gluten senzitivna enteropatija ili celijaÄna bolest je danas znatno ÄeÅ”Äa bolest nego Å”to se ranije smatralo. Gluten senzitivna enteropatija je nasledna bolest koja se karakteriÅ”e inflamacijom sluznice tankog creva nastale kao imunoloÅ”ki odgovor na gluten iz pÅ”enice, raži, jeÄma. Eliminacija glutena iz ishrane predstavlja medicinsku nutritivnu terapiju, odnosno terapiju izbora kada je ova bolest u pitanju. Dijeta bez glutena je veoma efikasna za ublažavanje simptoma bolesti, kao i za redukciju inflamacije i poboljÅ”anja apsorptivne funkcije mukoze creva. MeÄutim, ovaj režim ishrane je veoma kompleksan i zahtevan. TakoÄe, striktno sprovoÄenje dijete i njeno dugoroÄno prihvatanje prevenira pojavu makronutritivnih i mikronutritivnih deficita. Sem toga, pravovremeno uvoÄenje dijete bez glutena spreÄava pojavu komplikacija kao Å”to je anemija, osteoporoza, maligne bolesti i druge autoimmune bolesti. Iako postoje mnogi pokuÅ”aji da se pronaÄe njena alternativa, medicinska nutritvna terapija za gluten senzitivnu enteropatiju joÅ” uvek ostaje terapija izbora u leÄenju ove bolesti
Synthesis of Lutetium(III) Complex With a Polyazamacrocyclic Ligand
RadijacionŠ¾ zraÄenje predstavlja specifiÄan naÄin onkoloÅ”kog leÄenja, kod kojeg se antitumorski efekat postiže dejstvom jonizujuÄeg zraÄenja.Visokoenergetsko jonizujuÄe zraÄenje oÅ”teÄuje genetski materijal Äelija tumorskog tkiva i tako ograniÄava ili onemoguÄuje njihovu sposobnost daljnjeg deljenja. Radioizotopi koji se koriste u leÄenju u veÄini sluÄajeva greneriÅ”u beta zraÄenje koje izaziva uniÅ”tenje obolelih Äelija. Interesovanje za razvoj 177Lu kao radiofarmaceutika može se pripisati podobnosti njegovih nuklearnih svojstava za terapeutsku upotrebu, emisija Ī²(-) i Ī³ zraÄenja i njegovo dobro kompleksiranje sa raznim ligandima. U ovom radu je prikazana preliminarna sinteza kompleksa Lu(III) sa poliazamakrocikliÄnim ligandom.Radiation is a specific method of oncological treatment, in which the action of ionizing radiation achieves the antitumor effect. High-energy ionizing radiation damages the genetic material of tumor tissue cells and thus limits or disables their ability to divide further. Radioisotopes used in treatment in most cases generate beta radiation that causes the destruction of diseased cells. The interest in developing 177Lu as a radiopharmaceutical can be attributed to the suitability of its nuclear properties for therapeutic use, the emission ofĪ²(-) and Ī³ radiation, and its good complexation with various ligands. This paper presents the preliminary synthesis of Lu(III) complex with a polyazamacrocyclic ligand.XXXII Simpozijum DruÅ”tva za zaÅ”titu od zraÄenja Srbije i Crne Gore, 4-6. oktobar 2023; Budva, Crna GoraProceedings: [https://vinar.vin.bg.ac.rs/handle/123456789/11602
- ā¦