Bioelectromagnetic Field Simulations Using High-Resolution Human Anatomy Models with the Finite Integration Technique

In this paper high-resolution body phantoms are used for numerical electromagnetic field dosimetry simulations. Suitable discrete field formulations based on the Finite Integration Technique (FIT) are presented for the simulation of both the low and high frequency field exposure. Numerical field simulations involve a low-frequency magneto-quasistatic field exposure of the baby phantom under a heating blanket and high frequency SAR simulations of a baby close to baby phone as well as a GSM mobile phone in front of a male phantom. The use of dispersive material models for the simulation of pulsed field exposure is described

Phenotypic variation of salmonella in host tissues delays eradication by antimicrobial chemotherapy

Antibiotic therapy often fails to eliminate a fraction of transiently refractory bacteria, causing relapses and chronic infections. Multiple mechanisms can induce such persisters with high antimicrobial tolerance in vitro, but their in vivo relevance remains unclear. Using a fluorescent growth rate reporter, we detected extensive phenotypic variation of Salmonella in host tissues. This included slow-growing subsets as well as well-nourished fast-growing subsets driving disease progression. Monitoring of Salmonella growth and survival during chemotherapy revealed that antibiotic killing correlated with single-cell division rates. Nondividing Salmonella survived best but were rare, limiting their impact. Instead, most survivors originated from abundant moderately growing, partially tolerant Salmonella. These data demonstrate that host tissues diversify pathogen physiology, with major consequences for disease progression and control

Disparate Impact of Oxidative Host Defenses Determines the Fate of Salmonella during Systemic Infection in Mice

Reactive oxygen and nitrogen species function in host defense via mechanisms that remain controversial. Pathogens might encounter varying levels of these species, but bulk measurements cannot resolve such heterogeneity. We used single-cell approaches to determine the impact of oxidative and nitrosative stresses on individual Salmonella during early infection in mouse spleen. Salmonella encounter and respond to both stresses, but the levels and impact vary widely. Neutrophils and inflammatory monocytes kill Salmonella by generating overwhelming oxidative stress through NADPH oxidase and myeloperoxidase. This controls Salmonella within inflammatory lesions but does not prevent their spread to more permissive resident red pulp macrophages, which generate only sublethal oxidative bursts. Regional host expression of inducible nitric oxide synthase exposes some Salmonella to nitrosative stress, triggering effective local Salmonella detoxification through nitric oxide denitrosylase. Thus, reactive oxygen and nitrogen species influence dramatically different outcomes of disparate Salmonella-host cell encounters, which together determine overall disease progression