ENABLING PLACE-BASED CLIMATE ACTION IN THE UK:RECOMMENDATIONS FROM THE PCAN EXPERIENCE

This is a summary of key findings and recommendations from a report published by PCAN that was presented at PCAN's conference, Local Climate Action: Moving Out of Silos, at The Royal Society on 19 June 2023.We present key findings on: how place-based climate action in the UK continues to grow; the new governance models emerging; the need for (and lack of) political leadership, and how local partnerships can lead to better outcomes.The summary has recommendations for local authorities; communities and stakeholders; businesses, and national government and devolved administrations.Contributing authors to the full report are listed below. Read the report here: https://pcancities.org.uk/report

Earthquake-triggered submarine canyon flushing transfers young terrestrial and marine organic carbon into the deep sea

Submarine canyons transfer substantial amounts of sediment and organic carbon (OC) into the deep ocean, nourishing deep-sea ecosystems and contributing to the global carbon cycle through OC burial and sequestration. Tracking lateral OC transport through submarine canyon systems is challenged by the deep-ocean setting, difficulties with constraining episodic depositional events, and the need to assess the composition and age of marine and terrestrial organic matter. We apply innovative parallel ramped pyrolysis oxidation-accelerator mass spectrometry and pyrolysis-gas chromatography-mass spectrometry with isotope analyses to track OC age and sources in the 2016 Kaikōura earthquake-triggered, canyon-flushing event that deposited along >1300 km of a submarine canyon-channel system, offshore Aotearoa New Zealand. Specifically, these techniques allow us to determine the ages, sources, and partitioning of OC within the Kaikōura turbidite deposit and test hypotheses of how submarine canyon systems contribute to lateral OC flux and burial. Our results show that, despite considerable canyon floor erosion, substantial amounts of young OC were flushed into the deep sea, with relatively little (∼2 %) pre-Holocene OC contributions. Even without a direct connection between rivers and submarine canyons, most (∼55 %) of the OC in the Kaikōura event bed is from terrestrial sources. However, the deposit also contains substantial amounts (∼22 %) of marine-derived OC and ∼23 % of the material is of unassignable origin. Particle sorting imparts variability on the age and composition of OC within turbidite deposits and along the turbidity current flow path. Terrestrial-derived OC is preferentially older than marine-derived OC and concentrated in coarser particle sizes found more commonly at the deposit base and in proximal settings. Young, marine-derived OC is concentrated at the surface of the deposits and tends to be enriched in finer particle sizes. Such OC partitioning in turbidites supports the relevance of depositional models for predicting and quantifying distribution of OC in deep-sea deposits. Earthquake-triggered, canyon flushing events and resulting turbidites enhance OC burial efficiency and can sequester OC effectively, contributing an important carbon sink to the sedimentary carbon cycle

Final report of the Place-Based Climate Action Network (PCAN)

The Place-Based Climate Action Network (PCAN) was a network funded by the Economic and Social Research Council (ESRC) that brought together the research community and decision-makers in the public, private and third sectors over the five-year period 2019 to 2024. The main aim of PCAN was to translate climate policy into action ‘on the ground’ in communities within the United Kingdom

Masculinities, Media and the Rugby Mind: An Analysis of Stakeholder Views on the Relationship Between Rugby Union, the Media, Masculine-Influenced Views on Injury, and Concussion

Rugby union, alongside other collision and contact sports, faces ever mounting pressure from increased recognition of concussive injuries and the risks they present to athletes, both in the short-term and long-term. Here, the media is a central component of increasing pressure for cultural change. This research analysed data from 524 self-selected survey respondents to examine rugby union fans’ and stakeholders’ perceptions of media portrayal of concussion and how it might influence their own perceptions. We found evidence of a complex and heterogenous relationship between perceptions of masculinity, views and attitudes toward mass media, and degree of involvement in rugby union. Specifically, partisans of the sport generally saw mass media as hostile, with coverage biased against rugby, allowing them to manufacture doubt regarding risk information, as well as maintaining involvement in the sport. We conclude that critical commentaries from the media have the ability to challenge masculinities around concussion

Long-term patterns of hillslope erosion by earthquake-induced landslides shape mountain landscapes

Widespread triggering of landslides by large storms or earthquakes is a dominant mechanism of erosion in mountain landscapes. If landslides occur repeatedly in particular locations within a mountain range, then they will dominate the landscape evolution of that section and could leave a fingerprint in the topography. Here, we track erosion provenance using a novel combination of the isotopic and molecular composition of organic matter deposited in Lake Paringa, New Zealand. We find that the erosion provenance has shifted markedly after four large earthquakes over 1000 years. Postseismic periods eroded organic matter from a median elevation of 722 +329/−293 m and supplied 43% of the sediment in the core, while interseismic periods sourced from lower elevations (459 +256/−226 m). These results are the first demonstration that repeated large earthquakes can consistently focus erosion at high elevations, while interseismic periods appear less effective at modifying the highest parts of the topography

Analytical validation of a next generation sequencing liquid biopsy assay for high sensitivity broad molecular profiling.

Circulating tumor DNA (ctDNA) analysis is being incorporated into cancer care; notably in profiling patients to guide treatment decisions. Responses to targeted therapies have been observed in patients with actionable mutations detected in plasma DNA at variant allele fractions (VAFs) below 0.5%. Highly sensitive methods are therefore required for optimal clinical use. To enable objective assessment of assay performance, detailed analytical validation is required. We developed the InVisionFirst™ assay, an assay based on enhanced tagged amplicon sequencing (eTAm-Seq™) technology to profile 36 genes commonly mutated in non-small cell lung cancer (NSCLC) and other cancer types for actionable genomic alterations in cell-free DNA. The assay has been developed to detect point mutations, indels, amplifications and gene fusions that commonly occur in NSCLC. For analytical validation, two 10mL blood tubes were collected from NSCLC patients and healthy volunteer donors. In addition, contrived samples were used to represent a wide spectrum of genetic aberrations and VAFs. Samples were analyzed by multiple operators, at different times and using different reagent Lots. Results were compared with digital PCR (dPCR). The InVisionFirst assay demonstrated an excellent limit of detection, with 99.48% sensitivity for SNVs present at VAF range 0.25%-0.33%, 92.46% sensitivity for indels at 0.25% VAF and a high rate of detection at lower frequencies while retaining high specificity (99.9997% per base). The assay also detected ALK and ROS1 gene fusions, and DNA amplifications in ERBB2, FGFR1, MET and EGFR with high sensitivity and specificity. Comparison between the InVisionFirst assay and dPCR in a series of cancer patients showed high concordance. This analytical validation demonstrated that the InVisionFirst assay is highly sensitive, specific and robust, and meets analytical requirements for clinical applications

Insights into the ecological impact of trout introduction in an oligotrophic lake using sedimentary environmental DNA

Introduced trout can induce trophic cascades, however, a lack of pre-introduction data limits knowledge on their impact in many lakes. Traditional paleolimnological approaches have been used to study historic species changes, but until recently these have been restricted to taxa with preservable body-parts. To explore the ecosystem effects of Salmo trutta (brown trout) introduction on an oligotrophic lake in Aotearoa-New Zealand, we used a multi-marker sedimentary environmental DNA (sedDNA) approach coupled with pigments to detect changes across multiple trophic levels. DNA was extracted from core depths capturing approximately 100 years before and after the expected arrival of S. trutta, and metabarcoding was undertaken with four primer sets targeting the 12S rRNA (fish), 18S rRNA (eukaryotes) and cytochrome c oxidase (COI; eukaryotes) genes. The earliest detection of S. trutta eDNA was 1906 (1892–1919 CE with 95% high probability density function) suggesting their introduction was shortly before this. Native fish diversity (12S and 18S rRNA) decreased after the detection of S. trutta, albeit the data was patchy. A shift in overall eukaryotic and algal communities (18S rRNA and COI) was observed around 1856 (1841–1871 CE) to 1891 (1877–1904 CE), which aligns with the expected S. trutta introduction. However, taxonomy could not be assigned to many of the 18S rRNA and COI sequences. Pigment concentrations did not change markedly after S. trutta introduction. SedDNA provides a new tool for understanding the impact of disturbances such as the introduction of non-native species; however, there are still several methodological challenges to overcome

An approach to understanding hydrologic connectivity on the hillslope and the implications for nutrient transport

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94780/1/gbc975.pd

Association of Circulating Tumor DNA Testing Before Tissue Diagnosis With Time to Treatment Among Patients With Suspected Advanced Lung Cancer: The ACCELERATE Nonrandomized Clinical Trial

IMPORTANCE: Liquid biopsy has emerged as a complement to tumor tissue profiling for advanced non-small cell lung cancer (NSCLC). The optimal way to integrate liquid biopsy into the diagnostic algorithm for patients with newly diagnosed advanced NSCLC remains unclear. OBJECTIVE: To evaluate the use of circulating tumor DNA (ctDNA) genotyping before tissue diagnosis among patients with suspected advanced NSCLC and its association with time to treatment. DESIGN, SETTING, AND PARTICIPANTS: This single-group nonrandomized clinical trial was conducted among 150 patients at the Princess Margaret Cancer Centre-University Health Network (Toronto, Ontario, Canada) between July 1, 2021, and November 30, 2022. Patients referred for investigation and diagnosis of lung cancer were eligible if they had radiologic evidence of advanced lung cancer prior to a tissue diagnosis. INTERVENTIONS: Patients underwent plasma ctDNA testing with a next-generation sequencing (NGS) assay before lung cancer diagnosis. Diagnostic biopsy and tissue NGS were performed per standard of care. MAIN OUTCOME AND MEASURES: The primary end point was time from referral to treatment initiation among patients with advanced nonsquamous NSCLC using ctDNA testing before diagnosis (ACCELERATE [Accelerating Lung Cancer Diagnosis Through Liquid Biopsy] cohort). This cohort was compared with a reference cohort using standard tissue genotyping after tissue diagnosis. RESULTS: Of the 150 patients (median age at diagnosis, 68 years [range, 33-91 years]; 80 men [53%]) enrolled, 90 (60%) had advanced nonsquamous NSCLC. The median time to treatment was 39 days (IQR, 27-52 days) for the ACCELERATE cohort vs 62 days (IQR, 44-82 days) for the reference cohort (P \u3c .001). Among the ACCELERATE cohort, the median turnaround time from sample collection to genotyping results was 7 days (IQR, 6-9 days) for plasma and 23 days (IQR, 18-28 days) for tissue NGS (P \u3c .001). Of the 90 patients with advanced nonsquamous NSCLC, 21 (23%) started targeted therapy before tissue NGS results were available, and 11 (12%) had actionable alterations identified only through plasma testing. CONCLUSIONS AND RELEVANCE: This nonrandomized clinical trial found that the use of plasma ctDNA genotyping before tissue diagnosis among patients with suspected advanced NSCLC was associated with accelerated time to treatment compared with a reference cohort undergoing standard tissue testing

Association of Circulating Tumor DNA Testing Before Tissue Diagnosis With Time to Treatment Among Patients With Suspected Advanced Lung Cancer: The ACCELERATE Nonrandomized Clinical Trial.

IMPORTANCE Liquid biopsy has emerged as a complement to tumor tissue profiling for advanced non-small cell lung cancer (NSCLC). The optimal way to integrate liquid biopsy into the diagnostic algorithm for patients with newly diagnosed advanced NSCLC remains unclear. OBJECTIVE To evaluate the use of circulating tumor DNA (ctDNA) genotyping before tissue diagnosis among patients with suspected advanced NSCLC and its association with time to treatment. DESIGN, SETTING, AND PARTICIPANTS This single-group nonrandomized clinical trial was conducted among 150 patients at the Princess Margaret Cancer Centre-University Health Network (Toronto, Ontario, Canada) between July 1, 2021, and November 30, 2022. Patients referred for investigation and diagnosis of lung cancer were eligible if they had radiologic evidence of advanced lung cancer prior to a tissue diagnosis. INTERVENTIONS Patients underwent plasma ctDNA testing with a next-generation sequencing (NGS) assay before lung cancer diagnosis. Diagnostic biopsy and tissue NGS were performed per standard of care. MAIN OUTCOME AND MEASURES The primary end point was time from referral to treatment initiation among patients with advanced nonsquamous NSCLC using ctDNA testing before diagnosis (ACCELERATE [Accelerating Lung Cancer Diagnosis Through Liquid Biopsy] cohort). This cohort was compared with a reference cohort using standard tissue genotyping after tissue diagnosis. RESULTS Of the 150 patients (median age at diagnosis, 68 years [range, 33-91 years]; 80 men [53%]) enrolled, 90 (60%) had advanced nonsquamous NSCLC. The median time to treatment was 39 days (IQR, 27-52 days) for the ACCELERATE cohort vs 62 days (IQR, 44-82 days) for the reference cohort (P < .001). Among the ACCELERATE cohort, the median turnaround time from sample collection to genotyping results was 7 days (IQR, 6-9 days) for plasma and 23 days (IQR, 18-28 days) for tissue NGS (P < .001). Of the 90 patients with advanced nonsquamous NSCLC, 21 (23%) started targeted therapy before tissue NGS results were available, and 11 (12%) had actionable alterations identified only through plasma testing. CONCLUSIONS AND RELEVANCE This nonrandomized clinical trial found that the use of plasma ctDNA genotyping before tissue diagnosis among patients with suspected advanced NSCLC was associated with accelerated time to treatment compared with a reference cohort undergoing standard tissue testing. TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT04863924