El moho blanco

White mold, caused by the fungus Sclerotinia (Whetzelinia) sclerotiorum, is distributed worldwide and has more than 300 hosts. It infects flowers, cotyledons, seeds, leaves or injured plant tissue. The disease can be controlled by crop rotation, flooding, reduced seeding rates, application of chemical products in the middle of the flowering period, modifying plant architecture and the use of resistant var. Many soil microorganisms are associated with sclerotia and may cause them to degrade or fan to germinate. The symptoms and damage caused by the disease are illustrated in color. (CIAT)El moho blanco causado por el hongo Sclerotinia (Whetzelinia) sclerotiorum se halla presente en todo el mundo y mas de 300 plantas son hospedantes del patogeno; infecta las flores, cotiledones, semillas, hojas o tejido herido. El control de la enfermedad se puede hacer por: rotacion de cultivos, inundacion del terreno, utilizacion de menor cantidad de semilla en la siembra, aplicacion de productos quimicos a mediados de la floracion, modificando la arquitectura de la planta y utilizando var resistentes; muchos microorganismos del suelo estan asociados con los esclerosios y pueden causar degradacion e impedir que estos germinen. Se ilustran en color los sintomas y danos causados por la enfermedad. (CIAT

White mold

White mold, caused by the fungus Sclerotinia (Whetzelinia) sclerotiorum, is distributed worldwide and has more than 300 hosts. It infects flowers, cotyledons, seeds, leaves, or injured plant tissue. The disease can be controlled by crop rotation, flooding, reduced seeding rates, fewer irrigations, and destruction of bean- cull screenings containing sclerotia. Other control measures include chemical products in the middle of the flowering period, modifying plant architecture, and using resistant var. Many soil microorganisms are associated with sclerotia and may cause them to degrade or fail to germinate. The symptoms and damage caused by the disease are illustrated in color. (CIAT)El moho blanco causado por el hongo Sclerotinia (Whetzelinia) sclerotiorum se halla presente en todo el mundo, con mas de 300 plantas hospedantes del patogeno; infecta las flores, cotiledones, semillas, hojas o tejido lesionado. El control de la enfermedad se puede hacer por rotacion de cultivos, inundacion del terreno, menos riego y destruccion de residuos con esclerocios y utilizacion de menor cantidad de semilla en la siembra. Otras medidas de control incluyen la aplicacion de productos quimicos a mediados de la floracion, la modificacion de la arquitectura de la planta y la utilizacion de var. resistentes. Muchos microorganismos del suelo se asocian con los esclerocios y pueden causar degradacion e impedir que estos germinen. Se ilustran a color los sintomas y danos causados por la enfermedad. (CIAT

\u3ci\u3eUromyces appendiculatus\u3c/i\u3e in Honduras: Pathogen Diversity and Host Resistance Screening

Bean rust, caused by the fungus Uromyces appendiculatus, is a major constraint for common bean production worldwide. Virulence of U. appendiculatus collected from wild and cultivated Phaseolus spp. was examined in 28 locations across Honduras. Host accessions representing wild and domesticated Phaseolus spp. collected at the same sampling locations were evaluated for resistance against U. appendiculatus. In total, 91 pathotypes were identified from 385 U. appendiculatus isolates according to their virulence on each of the 12 host differentials. No significant difference in pathogen total virulence, measured as the mean disease score, was found between locations. However, significant differences were found in pathotype virulence among isolates collected from different Phaseolus spp. within a location. Moreover, when locations were compared on the basis of pathotype occurrence and frequency, differences among locations were evident. No two locations had the same pathotype composition. The most common pathotype was virulent on 9 of the 12 differential lines. A high number of resistant accessions were identified in Phaseolus coccineus and P. lunatus. Although most wild P. vulgaris accessions were highly susceptible, rust resistance was observed in P. vulgaris landraces collected from farmer’s fields. Thirty-two (52%) of the accessions screened showed intermediate to high levels of resistance and, of those, 16% were P. coccineus accessions. Our findings support the hypothesis that interaction of U. appendiculatus in host populations composed of diverse Phaseolus spp. and genotypes has favored highly diverse and virulent pathotypes, creating a center for virulence diversity of the pathogen in Honduras. The high percentage of intermediate and highly resistant accessions identified in the present study supports the strategy of collecting plants from the center of diversity of a pathogen or in locations with high incidence of disease and pathogen diversity to maximize the probability of identifying new sources of resistance

COMPARING MEASURING METHODS USING THE SENSITIVITY RATIO: AN APPLICATION TO RESISTANCE SCREENING IN SOYBEANS

When there are several methods of measuring a physical or chemical property, it is necessary to determine which method is best. If both methods are measured on the same scale, the most precise method will be preferred. However, often the methods have different scales. The sensitivity ratio allows for explicit comparison of methods with different scales. We use the sensitivity ratio to compare soybean resistance screening methods to evaluate the resistance of soybean varieties to Sclerotinia sclerotiorum. When compared to the root mean square error or the coefficient of variation, the sensitivity ratio can order methods differently both when the methods are measured on different scales and on the same scale. Our results cast doubt upon using standard precision statistics such as the root mean square error or the coefficient of variation to compare measuring methods and we suggest that the sensitivity ratio should be used instead

Population structure and phenotypic variation of \u3ci\u3eSclerotinia sclerotiorum\u3c/i\u3e from dry bean (\u3ci\u3ePhaseolus vulgaris\u3c/i\u3e) in the United States

The ascomycete pathogen Sclerotinia sclerotiorum is a necrotrophic pathogen on over 400 known host plants, and is the causal agent of white mold on dry bean. Currently, there are no known cultivars of dry bean with complete resistance to white mold. For more than 20 years, bean breeders have been using white mold screening nurseries (wmn) with natural populations of S. sclerotiorum to screen new cultivars for resistance. It is thus important to know if the genetic diversity in populations of S. sclerotiorum within these nurseries (a) reflect the genetic diversity of the populations in the surrounding region and (b) are stable over time. Furthermore, previous studies have investigated the correlation between mycelial compatibility groups (MCG) and multilocus haplotypes (MLH), but none have formally tested these patterns.We genotyped 366 isolates of S. sclerotiorum from producer fields and wmn surveyed over 10 years in 2003–2012 representing 11 states in the United States of America, Australia, France, and Mexico at 11 microsatellite loci resulting in 165 MLHs. Populations were loosely structured over space and time based on analysis of molecular variance and discriminant analysis of principal components, but not by cultivar, aggressiveness, or field source. Of all the regions tested, only Mexico (n = 18) shared no MLHs with any other region. Using a bipartite network-based approach, we found no evidence that the MCGs accurately represent MLHs. Our study suggests that breeders should continue to test dry bean lines in several wmn across the United States to account for both the phenotypic and genotypic variation that exists across regions

Registration of Great Northern Common Bean Cultivar ‘Coyne’ with Enhanced Disease Resistance to Common Bacterial Blight and Bean Rust

Great northern common bean (Phaseolus vulgaris L.) ‘Coyne’ (Reg. No. CV-287, PI 655574) was developed by the dry bean breeding program at the University of Nebraska Agricultural Research Division and released in 2008. It was bred specifically for adaptation to Nebraska growing conditions and for enhanced resistance to common bacterial blight (CBB), a major disease of common bean caused by the seed-borne bacterium Xanthomonas campestris pv. phaseoli (Smith) Dye, and bean common rust Uromyces appendiculatus (Pers.:Pers) Unger. Coyne is a great northern F7:8 line derived from a three-way cross (G95023/Weihing//BelMiNeb-RMR-11). The first cross was made in winter 2003. The F7:8 was tested in advanced yield trials at Scottsbluff and Mitchell, NE, and in growers’ fields in Nebraska. Yield of Coyne was only 47 kg ha–1 lower than ‘Marquis’ in Morrill and Scotts Bluff, NE, counties. Reaction of Coyne to CBB under field conditions was consistent across 3 yr at the West Central Research and Extension Center, North Platte, NE, where fi eld disease ratings of 3.2, 3.5, and 4.4 were recorded in 2005, 2006, and 2007, respectively. Coyne has the Ur-3 and Ur-6 genes for resistance to common bean rust and carries the single dominant hypersensitive I gene that provides resistance to all non-necrotic strains of Bean common mosaic virus. Coyne has bright white seed, blooms 44 d after planting, and is a midseason bean, maturing 91 d after planting

Root and crown rot pathogens found on dry beans grown in Mozambique

Dry edible beans are a vital food source in Mozambique, East Africa—one that alleviates hunger and malnutrition and adds value to the economy. In recent years, root/crown rot (RCR) pathogens have emerged as limiting constraints in dry bean production. Not much has been characterized concerning the causal agents of RCR in Mozambique. The purpose of this study was to identify the primary pathogen(s) associated with RCR dry bean samples collected at breeder nursery sites and farmer fields in Mozambique using molecular sequencing and culture-based methods. Sequencing revealed, not surprisingly, an increased diversity of fungal/oomycete operational taxonomic units when compared to culture-based methods of diversity. Species of Fusarium, mainly F. oxysporum, were the dominant taxa detected in RCR dry beans through sequencing the ITS rDNA region and partial EF-1α gene. Collectively, 333 fungi and/or Oomycetes were isolated in culture during the 2014–2015 growing seasons and tested for pathogenicity on healthy bean seedlings. Fusarium species were identified by both morphological and molecular characters. At least 60% of the isolates inoculated on common bean were recognized as potentially pathogenic. From both isolation frequency and pathogenicity testing, F. oxysporum and related species play an important role in the bean RCR complex. We found similar results from dry beans grown in the two main bean-growing regions of Mozambique. These findings will allow breeders to screen for resistance to F. oxysporum in greenhouse grown bean plants as well as within field grown bean cultivars

Registration of Common Bacterial Blight, Rust and Bean Common Mosaic Resistant Great Northern Common Bean Germplasm Line ABC-Weihing

Great northern common bean (Phaseolus vulgaris L.) germplasm line ABC-Weihing (Reg. No. GP-246, PI 647964) was developed by the University of Nebraska Agricultural Research Division in cooperation with USDA-ARS and released in 2006. This line, tested as NE1-05-4, was bred specifically for enhanced resistance to common bacterial blight (CBB), a major seed borne disease of common bean caused by the bacterium Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp). ABC-Weihing is a great northern BC5F3:6 line obtained from five backcrosses (‘Weihing’*5//‘Chase’/XAN 159). The first cross was made in spring 1997. Only BCnF1 plants resistant to Xcp isolates Dominican Republic DR-7 and Nebraska SC4A, as determined by multiple needle leaf inoculation tests in the greenhouse, were used for successive backcrossing. In addition to phenotypic selection for CBB resistance, marker-assisted selection for the resistant QTLlinked marker SU91 was conducted in the BC1F1, BC2F1, and ABC-Weihing. When inoculated with Nebraska Xcp strains in the field, ABC-Weihing exhibited resistance in both 2005 and 2006. ABC-Weihing has Ur-3 and Ur-6 genes for resistance to common bean rust and carries the single dominant hypersensitive I gene that provides resistance to all non-necrotic strains of the Bean common mosaic virus (BCMV). ABC-Weihing has bright white seed, blooms 45 d after planting, and is a midseason bean maturing 92 d after planting

Symptoms associated with victimization in patients with schizophrenia and related disorders

Background: Patients with psychoses have an increased risk of becoming victims of violence. Previous studies have suggested that higher symptom levels are associated with a raised risk of becoming a victim of physical violence. There has been, however, no evidence on the type of symptoms that are linked with an increased risk of recent victimization. Methods: Data was taken from two studies on involuntarily admitted patients, one national study in England and an international one in six other European countries. In the week following admission, trained interviewers asked patients whether they had been victims of physical violence in the year prior to admission, and assessed symptoms on the Brief Psychiatric Rating Scale (BPRS). Only patients with a diagnosis of schizophrenia or related disorders (ICD-10 F20–29) were included in the analysis which was conducted separately for the two samples. Symptom levels assessed on the BPRS subscales were tested as predictors of victimization. Univariable and multivariable logistic regression models were fitted to estimate adjusted odds ratios. Results: Data from 383 patients in the English sample and 543 patients in the European sample was analysed. Rates of victimization were 37.8% and 28.0% respectively. In multivariable models, the BPRS manic subscale was significantly associated with victimization in both samples. Conclusions: Higher levels of manic symptoms indicate a raised risk of being a victim of violence in involuntary patients with schizophrenia and related disorders. This might be explained by higher activity levels, impaired judgement or poorer self-control in patients with manic symptoms. Such symptoms should be specifically considered in risk assessments

Structural Analysis and Development of Notum Fragment Screening Hits

The Wnt signaling suppressor Notum is a promising target for osteoporosis, Alzheimer's disease, and colorectal cancers. To develop novel Notum inhibitors, we used an X-ray crystallographic fragment screen with the Diamond-SGC Poised Library (DSPL) and identified 59 fragment hits from the analysis of 768 data sets. Fifty-eight of the hits were found bound at the enzyme catalytic pocket with potencies ranging from 0.5 to >1000 μM. Analysis of the fragments' diverse binding modes, enzymatic inhibitory activities, and chemical properties led to the selection of six hits for optimization, and five of these resulted in improved Notum inhibitory potencies. One hit, 1-phenyl-1,2,3-triazole 7, and its related cluster members, have shown promising lead-like properties. These became the focus of our fragment development activities, resulting in compound 7d with IC50 0.0067 μM. The large number of Notum fragment structures and their initial optimization provided an important basis for further Notum inhibitor development