551 research outputs found

    Self-determination in a work organization.

    Get PDF

    CAPG is required for Ebola virus infection by controlling virus egress from infected cells

    Get PDF
    The replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors involved in actin nucleation, such as Rac1 and Arp2/3, were shown important in both steps. However, downstream of nucleation, many other cell factors are needed to control actin dynamics. How these regulate EBOV infection remains largely unclear. Here, we identified the actin-regulating protein, CAPG, as important for EBOV replication. Notably, knockdown of CAPG specifically inhibited viral infectivity and yield of infectious particles. Cell-based mechanistic analysis revealed a requirement of CAPG for virus production from infected cells. Proximity ligation and split-green fluorescent protein reconstitution assays revealed strong association of CAPG with VP40 that was mediated through the S1 domain of CAPG. Overall, CAPG is a novel host factor regulating EBOV infection through connecting actin filament stabilization to viral egress from cells

    Perceived locus of causality and internalization: Examining reasons for acting in two domains.

    Get PDF
    Theories of internalization typically suggest that self-perceptions of the "causes" of(i.e., reasons for) behavior are differentiated along a continuum of autonomy that contains identifiable gradations. A model of perceived locus of causality (PLOC) is developed, using children's self-reported reasons for acting. In Project 1, external, introjected, identified, and intrinsic types of reasons for achievementrelated behaviors are shown to conform to a simplex-like (ordered correlation) structure in four samples. These reason categories are then related to existing measures of PLOC and to motivation. A second project examines 3 reason categories (external, introject, and identification) within the domain of prosoeial behavior. Relations with measures of empathy, moral judgment, and positive interpersonal relatedness are presented. Finally, the proposed model and conceptualization of PLOC are discussed with regard to intrapersonal versus interpersonal perception, internalization, causereason distinctions, and the significance of perceived autonomy in human behavior. A central issue for theories of motivation concerns the perceived locus relative to the person of variables that cause or give impetus to behavior, Heider (1958) introduced the concept of perceived locus of causality (PLOC) primarily in reference to interpersonal perception, and more specifically with regard to the phenomenal analysis of how one infers the motives and intentions of others. He distinguished between personal causation, the critical feature of which is intention, and impersonal causation, in which environments, independent of the person's intentions, produce a given effect. DeCharms (1968) elaborated and extended Heider's phenomenal analysis, particularly with regard to the explanation of behavior (as opposed to outcomes). DeCharms argued that there is a further distinction within personal causation or intentional behavior between an internal PLOC, in which the actor is perceived as an "origin" of his or her behavior, and an external PLOC, in which the actor is seen as a "pawn" to heteronomous forces. The distinction between internal and external PLOC has since been crucial for studies of intrinsic versus extrinsic motivation and of perceived autonomy more generally (Deci &amp

    Finding footy : female fan socialization and Australian rules football

    Full text link
    The question of how, irrespective of gender, a person becomes a sports fan has been absent in sociological studies of sports supporters. Distinct from other studies of sport spectatorship that focus on the practices of already existing (and overwhelmingly male) fans, our research is the first to consider how women become supporters, and in doing so, it begins to redress the significant under-representation of women in sports fan research. From interviews with female supporters of the Australian Football League (AFL), this article identifies and critically assesses the modes by which women come to support sport. We propose four categories to explain the different ways women accomplish fandom, focusing on the importance of strong social ties and doxic actions in this process. The events, experiences and social relations that inform women\u27s first encounters with AFL offers a template for the wider consideration of women as social agents in the sporting landscape

    A Cognitive Model of an Epistemic Community: Mapping the Dynamics of Shallow Lake Ecosystems

    Full text link
    We used fuzzy cognitive mapping (FCM) to develop a generic shallow lake ecosystem model by augmenting the individual cognitive maps drawn by 8 scientists working in the area of shallow lake ecology. We calculated graph theoretical indices of the individual cognitive maps and the collective cognitive map produced by augmentation. The graph theoretical indices revealed internal cycles showing non-linear dynamics in the shallow lake ecosystem. The ecological processes were organized democratically without a top-down hierarchical structure. The steady state condition of the generic model was a characteristic turbid shallow lake ecosystem since there were no dynamic environmental changes that could cause shifts between a turbid and a clearwater state, and the generic model indicated that only a dynamic disturbance regime could maintain the clearwater state. The model developed herein captured the empirical behavior of shallow lakes, and contained the basic model of the Alternative Stable States Theory. In addition, our model expanded the basic model by quantifying the relative effects of connections and by extending it. In our expanded model we ran 4 simulations: harvesting submerged plants, nutrient reduction, fish removal without nutrient reduction, and biomanipulation. Only biomanipulation, which included fish removal and nutrient reduction, had the potential to shift the turbid state into clearwater state. The structure and relationships in the generic model as well as the outcomes of the management simulations were supported by actual field studies in shallow lake ecosystems. Thus, fuzzy cognitive mapping methodology enabled us to understand the complex structure of shallow lake ecosystems as a whole and obtain a valid generic model based on tacit knowledge of experts in the field.Comment: 24 pages, 5 Figure
    • …
    corecore