3 research outputs found
Synthesis of poly ? ketoesters via double acylketene trapping
The synthesis and characterization of polymeric ? ketoesters is herein reported. These polymers were prepared by allowing highly electrophilic acylketenes, generated in situ via thermolysis of stable 6-substituted-2,2-dimethyl-4H-1,3-dioxin-4-ones, to react with diol co-monomers. A relationship between polymer molecular weights and reaction conditions was established
Systematic comparison of single-walled carbon nanotube/poly(vinyl acetate) graft-to reactions
Bioresponsive Small Molecule Polyamines as Noncytotoxic Alternative to Polyethylenimine
Nonviral gene therapy continues to require novel synthetic vectors to deliver therapeutic nucleic acids effectively and safely. The majority of synthetic nonviral vectors employed in clinical trials to date have been cationic liposomes; however, cationic polymers are attracting increasing attention. One of the few cationic polymers to enter clinical trials has been polyethylenimine (PEI); however, doubts remain over its cytotoxicity, and in addition it displays lower levels of transfection than viral systems. Herein, we report on the development of a series of small molecule analogues of PEI that are bioresponsive to the presence of pDNA, forming poly(disulfide)s that are capable of efficacious transfection with no associated toxicity. The most effective small molecule developed, a cyclic disulfide based upon a spermine backbone, is shown to form very well-defined polyplexes (100−200 nm in diameter) that mediate murine lung transfection in vivo to within an order of magnitude of in vivo jetPEI, and at the same time display a much improved cytotoxicity profile