ORAL CREATINE SUPPLEMENTATION AND SHORT-TERM DYNAMIC POWER PRODUCTION IN HEALTHY YOUNG MEN

This experiment examined the effect of creatine monohydrate supplementation on corrected peak power output and peak acceleration during repeated, high intensity sprint cycling. The investigation was randomised, placebo-controlled, double-blind and adopted a crossover design. Eight male, adult volunteers participated in this study. Subjects were administered with creatine monohydrate (0.28g.kg-1 per day) or a glucose placebo. Following experimental treatments, subjects underwent 10 maximal effort, 6-s sprints on a cycle ergometer with a work to rest ratio of 5:1. The exercise protocol was fatiguing in nature with peak power output and peak acceleration decreasing significantly from the first to last sprint. Creatine supplementation significantly attenuated the decline in peak power output in the latter stages of the intermittent sprint cycling

Measurement of muscle health in aging

Muscle health is a critical component in the struggle against physical frailty and the efforts to maintain metabolic health until the limit of chronological age. Consensus opinion is to evaluate muscle health in terms of muscle mass, strength and functional capability. There has been considerable variability in the components of muscle health which have been investigated in addition to variability in the tools of assessment and protocol for measurement. This is in stark contrast to the validated measurement of bone health across the adult life span. The purpose of this review was to identify indices of muscle mass, strength and functional capability most responsive to change with ageing and where possible to provide an estimate of the rate of change. We suggest lean tissue mass (LTM) or skeletal muscle (SM) is best evaluated from the thigh region due to its greater responsiveness to ageing compared to the whole body. The anterior compartment of the thigh region undergoes a preferential age-related decline in SM and force generating capacity. Therefore, we suggest that knee extensor torque is measured to represent the force generating capacity of the thigh and subsequently, to express muscle quality (strength per unit tissue). Finally, we suggest measures of functional capability which allow participants perform to a greater maximum are most appropriate to track age-related difference in functional capacity across the adult lifespan. This is due to their ability encompass a broad spectrum of abilities. This review suggests indices of muscular health for which reference ranges can be generated across the lifespan

Use of catchment attributes to identify the scale and values of distributed parameters in surface and sub-surface conceptual hydrology models

Improved prediction for problems in catchment hydrology requires an ability to spatially disaggregate and connect surface and sub-surface components. This paper considers two hydrological models for use in such disaggregation and coupling: a lumped conceptual rainfall-runoff model (IHACRES) and a physics based conceptual groundwater discharge model. Smaller gauged catchments in the vicinity can be used to regionalise and parameterise the coupled model using catchment attributes prior to running the model in a larger catchment with fewer gauges. Regionalisation in gauged catchments at appropriate scales would capture the uncertainty of the relationships between catchment attributes and model parameter values, including the upper and lower boundary of parameter values. In an ungauged and disaggregated catchment, its landscape attributes would be inserted into the regional relationships to provide the parameter bounds for constraining the proposed coupled model. The aim of this catchment disaggregation is to be able to improve on previous catchment or sub-catchment recharge-discharge models, so that modelling can be carried out at the management scale

Potential for using simulated altitude as a means of prehabilitation:a physiology study

The current pandemic of surgical complications necessitates urgent and pragmatic innovation to reduce postoperative morbidity and mortality, which are associated with poor pre-operative fitness and anaemia. Exercise prehabilitation is a compelling strategy, but it has proven difficult to establish that it improves outcomes either in isolation or as part of a multimodal approach. Simulated altitude exposure improves performance in athletes and offers a novel potential means of improving cardiorespiratory and metabolic fitness and alleviating anaemia within the prehabilitation window. We aimed to provide an initial physiological foundation for ‘altitude prehabilitation’ by determining the physiological effects of one week of simulated altitude (FIO2 15%, equivalent to approximately 2438 m (8000 ft)) in older sedentary volunteers. The study used a randomised, double-blind, sham-controlled crossover design. Eight participants spent counterbalanced normoxic and hypoxic weeks in a residential hypoxia facility and underwent repeated cardiopulmonary exercise tests. Mean (SD) age of participants was 64 (7) y and they were unfit, with mean (SD) baseline anaerobic threshold 12 (2) ml.kg-1.min-1 and mean (SD) peak V̇O2 15 (3) ml.kg-1.min-1. Hypoxia was mild (mean (SD) SpO2 93 (2) %, p &lt; 0.001) and well-tolerated. Despite some indication of greater peak exercise capacity following hypoxia, overall there was no effect of simulated altitude on anaerobic threshold or peak V̇O2. However, hypoxia induced a substantial increase in mean (SD) haemoglobin of 1.5 (2.7) g.dl-1 (13% increase, p = 0.028). This study has established the concept and feasibility of ‘altitude prehabilitation’ and demonstrated specific potential for improving haematological fitness. Physiologically, there is value in exploring a possible role for simulated altitude in pre-operative optimisation.</p

Techniques for assessing the performance of a landscape-based sediment source and transport model: sensitivity trials and physical methods

Widespread degradation of aquatic habitat and water quality has occurred since European settlement of Australia. Repairing this degradation is expensive and hence on-ground management needs to be carefully focussed. The Sediment River Network model, SedNet, used for the estimation of the sources and transport of sediment spatially and at catchment scales, potentially provides a useful tool to assist land managers in focusing this work. The complete model, whilst broadly applied has not been systematically tested to assess its accuracy or sensitivity to its various model components. The aim of this paper is to propose a framework for such testing. Results from the work will be used to prioritise data acquisition, and improve the structure and parameterisation of the model where necessary. The research is also particularly relevant for shifting application of the model from continental to catchment scales. The testing will comprise two components - sensitivity assessment and accuracy assessment. This paper provides a brief introduction to the SedNet model and a framework for assessing the model. Examples of sensitivity assessment and accuracy assessment are provided and discussed

Raman Spectroscopy of the Human Nail: A Potential Tool for Evaluating Bone Health?

Dual X-ray absorptiometry (DEXA) is the current gold standard for the diagnosis of osteoporosis. However, patients can suffer osteoporotic fractures despite normal bone mineral density, partly because of unmeasured influences of both the protein and mineral phases of bone that are affected in osteoporosis. There is currently no clinically applicable method of evaluating the health of the protein phase. The proteins in human nail (keratin) and bone (collagen) require sulphation and disulphide bond (S-S) formation for structural integrity and disorders of either sulphur metabolism or cystathione beta-synthase can lead to structural abnormalities in these tissues. Raman protein spectra provide a method of non-invasive measurement of the degree of sulphation of structurally related proteins that may be indicative of bone health. Raman spectroscopy was used to evaluate the disulphide (S-S) content of fingernails. The nail samples came from from 169 women (84 pre- and 85 post-menopausal), of which 39 had a history of osteoporotic fracture. BMD was measured by DXA at the spine. Analyses included parametric and non-parametric tests, dependent on the distribution of the test variable. Mean disulphide content of the nail reduced with age and was slightly higher in pre-, compared to post-menopausal women (P = 0.187). Significantly lower disulphide content was observed in nails obtained from subjects with a history of fracture (P = 0.025). When either disulphide content or BMD was used as a predictor, the odds ratio of these two measures were found to be comparable predictors for fracture status. This suggests that measurements of change in the protein phase of structural proteins such as keratin in the human nail may be correlated with clinically relevant changes in bone proteins that are important in fracture risk. © 2007 Springer Science+Business Media, LLC

Studies on Calf Diarrhoea in Mozambique: Prevalence of Bacterial Pathogens

The prevalence of diarrhoea in calves was investigated in 8 dairy farms in Mozambique at 4 occasions during 2 consecutive years. A total of 1241 calves up to 6 months of age were reared in the farms, and 63 (5%) of them had signs of diarrhoea. Two farms had an overall higher prevalence (13% and 21%) of diarrhoea. Faecal samples were collected from all diarrhoeal calves (n = 63) and from 330 healthy calves and analysed for Salmonella species, Campylobacter jejuni and enterotoxigenic Escherichia coli (ETEC). Salmonella spp. was isolated in only 2% of all calves. Campylobacter was isolated in 11% of all calves, irrespective of health condition, and was more frequent (25%) in one of the 2 diarrhoeal farms (p = 0.001). 80% of the isolates were identified as C. jejuni. No ETEC strains were detected among the 55 tested strains from diarrhoeal calves, but 22/55 (40%) strains from diarrhoeal calves and 14/88 (16%) strains from healthy calves carried the K99 adhesin (p = 0.001). 6,757 E. coli isolates were typed with a biochemical fingerprinting method (the PhenePlate™) giving the same E. coli diversity in healthy and diarrhoeal calves. Thus it was concluded: i) the overall prevalence of diarrhoea was low, but 2 farms had a higher prevalence that could be due to an outbreak situation, ii) Salmonella did not seem to be associated with diarrhoea, iii) Campylobacter jejuni was common at one of the 2 diarrhoeal farms and iv) ETEC strains were not found, but K99 antigen was more prevalent in E. coli strains from diarrhoeal calves than from healthy, as well as more prevalent in one diarrhoeal farm