183 research outputs found
Verejtékmirigy adenoma és simplex lipoma komponensű benignus compositiós vegyes daganat esete kutyában = A case of benign compository mixed tumour with sweet-gland adenoma and simple lipoma components in a dog
A szerzők egy 11 éves golden retriever szuka kutya bal lapockatájékáról származó verejtékmirigy adenoma és simplex lipoma komponensekből felépülő, benignus compositiós daganat kórszövettani és immunhisztokémiai feldolgozását mutatják be. A kb. 2×2,5 cm átmérőjű, közepesen tömött tapintatú, subcutan helyeződésű, fájdalmatlan, elmozdítható daganat preaoperativ cytologiai vizsgálat során simplex lipomára utaló sejtpopulációt figyeltek meg. A kórszövettani vizsgálata során differenciált, fehérzsírszöveti sejtek, körülírt, fibroticus tokkal demarkált, jóindulatú burjánzását figyelték meg. A jóindulatú zsírszöveti daganat központjában kb. 1,5mm haránt- és kb. 6 mm hosszanti átmérőjű, solitaer, tubulopapillaris szöveti szerkezetű, simplex verejtékmirigy adenomát észleltek. Az immunhisztokémiai vizsgálat során a verejtékmirigy adenoma-sejtjei pancytokeratin cytoplasmaticus-, claudin-5 membrán-pozitivitást, vimentin-, α-SMA- és S-100 protein-negativitást mutattak. A szakirodalmi adatok alapján első esetleírásról van szó. A szerzők a daganat elnevezésére javasolják az adenolipoma nomenklatúrát, ill. a human dermato-onkológiában használatos, cutan apocrin adenolipoma elnevezésének újra értelmezését, ui. ez utóbbi lipoma állományában nem daganatos, elváltozásmentes verejtékmirigyek észlelhetők
A galgahévízi láprét talajtani, hidrológiai és szedimentológiai vizsgálata
Napjainkban fokozott figyelem irányul a vizes élőhelyek felé. Hazánkban jelentősen
lecsökkent a területük, szigetszerűvé váltak, éppen ezért, a természetes közegben betöltött
egyedülálló ökológiai szerepüknek köszönhetően egyre inkább az érdeklődés és a kutatások
középpontjába kerülnek (Ángyán et al. 2004).
Galgahévíz a Gödöllői-dombság és az észak-alföldi hordalékkúp síkság határán
helyezkedik el, a Galga-patak mentén. A területet Ny-ról határolja a Sósi-patak, amely egykor
a lápréten keresztül jutott a Galga-patakba.
A láprét természeti értékeinek degradációja (amelyet korábban a legeltetés és a
kaszálás miatt jelentős volt) az 1990-es évek végén felerősödött, eltűnőben vannak egyes,
természetvédelmi szempontból értékes növényfajok és társulások.
Célunk az volt, hogy feltárjuk ezen degradációs folyamatok talajtani, hidrológiai,
okait, a láprétet veszélyeztető tényezőket. A láprét kialakulását meghatározó egyik, talán a
legfontosabb tényező a víz, ezért fennmaradásához alapvetően fontos a vízutánpótlás
biztosítása. A láprét vízháztartásának, vízutánpótlásának megismerése, megértése
megalapozhatja a revitalizációs terveket.
A láprét kutatása az 1990-es évek végén kezdődött, azóta számos publikáció született
az itt feltárt eredményekről
Comparison of soil erosion dynamics under extensive and intensive cultivation based on basic soil parameters
The conservation of soil and its elemental feature, t
he soil fertility has not just national and
agricultural business interest, since the fertile soil is
an elemental part of the material turnover in the
environment. The soil erosion can reach huge spatial e
xtensions and starts with the most valuable part: the
upper, fertile layer. So, its research is crucial for
our future survival. Our studied area can be found
in
Gerézdpuszta at the Koppány
-valley of Hungary, which is a sensitive erosion area.
We sampled the upper 30
cm of soil layer in every 10 meters that resulted 32-32
point samples on the arable and on the grassland,
respectively. One sample was taken under the forest as r
eference. We analyzed the basic soil parameters:
CaCO
3
, pH, AL-P
2
O
5
, AL-K
2
O, Kuron’s higroscopicity, SOM and the particle size di
stribution was
determined. Besides, we examined the connection between
the given results from laboratory soil analyses and
the Munsell-type and Google Earth satellite image colors. O
ur results show the erosion dynamic on our
sample slope, and the main differences in basic soil paramet
ers between different intensities. The main
indicator of soil erosion dynamic were the CaCO
3
, the SOM, AL-P
2
O
5
and the particle size distribution,
where the SOM and CaCO
3
were correlate with the Munsell and Google Earth col
or also
Effects of land use types with different intensities on soil erosion, nutrient loss and other soil parameters
Expression of claudin-1, -3, -4, -5 and -7 proteins in low grade colorectal carcinoma of canines
The aim of the present study was to characterise the expression pattern of claudin-1, -3, -4, -5 and -7 tight junction proteins in canine normal colorectum and in the low-grade, tubulopapillary colorectal carcinoma in canines. Methods and results: The biopsy samples included 10 canine normal colorectal tissues and 20 canine low grade colorectal carcinomas (CLGCCs). The canine normal colorectal mucosa was negative for claudin-1. Claudin-1 was detected as a non-diffuse intense membrane labelling of neoplastic epithelial cells in low grade colorectal cancer in canines. Fifty five per cent of all tumours showed a weak cytoplasmic pattern of staining for claudin-1 protein. The normal colorectal mucosa showed diffuse punctate positivity for claudin-3. Claudin-3 was detected as an intense lateral membrane labelling of tumour cells in CLGCCs. Claudin-4 expression in surface and crypt epithelial cells of the intact colorectal mucosa in canines was punctate. Claudin-4 molecule was detected as a lateral membrane labelling of neoplastic cells in CLGCCs. The epithelium of the CLGCCs and the low grade colorectal carcinoma were negative for claudin-5. The surface and crypt epithlial cells of the canine normal colorectal mucosa showed a diffuse lateral membranous pattern of staining for claudin-7. Claudin-7 molecule was detected as an intense membrane labelling of neoplastic cells in CLGCCs. Seventy per cent of all tumours showed weak cytoplasmic positivity for claudin-7. Conclusion: Consequently, we hypothesize that claudin-1 plays a role in the progression of CLGCCs. Further functional studies are needed to clarify the biological role of the mislocalization of the claudin-1 molecule from cell membrane to the cytoplasm in CLGCCs. Lower claudin-4 expression suggests that reduced expression of claudin-4 molecule may lead to cellular disorientation, detachment and invasion of CLGCCs. Further functional studies are needed to clarify the biological role of overexpression and mislocalisation of claudin-7 in CLGCCs
Expression of claudins in the normal canine gastric mucosa
The aim of the present study was to investigate the expression pattern of claudin-1, -2, -3, -4, -5, -7, -8, -10 and -18 in the intact fundic and pyloric gastric mucosa of dogs. Intense, linear, membranous claudin-18 positivity was detected in the surface gastric cells and in the epithelial cells of the gastric glands both in the fundic and pyloric stomach regions. The mucous neck cells in the apical part of the glands, furthermore the parietal cells and chief cells of the basal part of the gland were all positive for claudin-18, in the same way as the enteroendocrine cells. Cells of the basal part of the pyloric glands showed intense, linear, membranous claudin-2 positivity, but cells of the superficial portion of these glands and the surface gastric cells in this region were claudin-2 negative. Fibroblasts, endothelial cells, lymphocytes of the propria layer, smooth muscle cells and vegetative neurons were all negative for claudin-2. All gastric epithelial cells were negative for claudin-1, -3, -4, -5, -6, -7, -8 and -10. The endothelial cells of the propria layer had intense claudin-5 positivity. We assume that claudin-18 forms a paracellular barrier against gastric acid in the healthy canine stomach, in the same way as in mice
Effect of cryogenic freezing on the textural and sensoric properties of donuts
Cryogenic freezing is a mild freezing technology due to the formation of small ice crystals. This preservation technology is well applicable for increasing the shelf life of bakery products. In our experiment, we made donut samples of matured dough, traditional dough with pork fat, and dough with increased fibre content made by adding flaxseed flour. They were fried in palm oil and also in high oleic sunflower oil. Donuts were frozen in liquid nitrogen for 60 seconds and they underwent a storage of 7 days at -18°C. Thawing at room temperature and thawing in oven were tested. We investigated how freezing, frozen storage and different thawing processes affect sample texture by penetration. The effect of freezing on water activity and sensory properties were also investigated. In our experiment we found that cryogenic freezing influenced the texture of prepared donuts, but sensory evaluation have shown that this does not lead to a decrease in popularity. In addition, a slight decrease in water activity was observed after freezing and thawing. Heating in oven proved to be the better method for the thawing process of donuts based on sensory properties of products. In addition, consumers preferred the increased fibre content of doughs
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