Vitamin D Status of Older Adults of Diverse Ancestry Living in the Greater Toronto Area

Background: Physiological and lifestyle factors put older adults at an increased risk of vitamin D insufficiency and resulting negative health outcomes. Here we explore the vitamin D status in a sample of community dwelling older adults of diverse ancestry living in the Greater Toronto area (GTA). Methods: Two hundred and twenty-four (224) adults over 60 years of age were recruited from the Square One Older Adult Centre, in Mississauga, Ontario. Circulating 25-hydroxyvitamin D (25(OH)D) concentrations were measured from dried blood spot cards. Dietary and supplemental intakes of vitamin D were assessed via questionnaires. Skin pigmentation was assessed quantitatively by measuring melanin levels using a reflectometer. Results: The mean 25(OH)D concentration in the total sample was 82.4 nmol/L. There were no statistically significant differences in serum 25(OH)D concentrations, supplemental or dietary vitamin D intakes between the three major ancestral groups (East Asians, Europeans and South Asians). Females had significantly higher 25(OH)D concentrations than males (84.5 nmol/L vs. 72.2 nmol/L, p = 0.012). The proportion of participants with 25(OH)D concentrations below 50 nmol/L and 75 nmol/L were 12.1%, and 38.8%, respectively. The mean daily supplemental intake of vitamin D was 917 IU/day. Vitamin D intake from supplements was the major factor determining 25(OH)D concentrations (p \u3c 0.001). Conclusions: Mean concentration of 25(OH)D in a sample of older adults of diverse ancestry living in the GTA exceeded 80 nmol/L, and there were no significant differences in 25(OH)D levels between ancestral groups. These results sharply contrast with our recent study focused on young adults of diverse ancestry living in the same geographic area, in which we found substantially lower 25(OH)D concentrations (mean 39.5 nmol/L), low supplemental vitamin D intake (114 IU/day), and significant differences in 25(OH)D levels between ancestral groups. High daily intake of supplemental vitamin D in this sample of older adults likely accounts for such disparate findings with respect to the young adult sample

Quantifying the Production of Fruit-Bearing Trees Using Image Processing Techniques

[EN] In recent years, the growth rate of world agricultural production and crop yields have decreased. Crop irrigation becomes essential in very dry areas and where rainfall is scarce, as in Egypt. Persimmon needs low humidity to obtain an optimal crop. This article proposes the monitoring of its performance, in order to regulate the amount of water needed for each tree at any time. In our work we present a technique that consists of obtaining images of some of the trees with fruit, which are subsequently treated, to obtain reliable harvest data. This technique allows us to have control and predictions of the harvest. Also, we present the results obtained in a first trial, through which we demonstrate the feasibility of using the system to meet the objectives set. We use 5 different trees in our experiment. Their fruit production is different (between 20 and 47kg of fruit). The correlation coefficient of the obtained regression model is 0.97.This work has been partially supported by European Union through the ERANETMED (Euromediterranean Cooperation through ERANET joint activities and beyond) project ERANETMED3-227 SMARTWATIR by the Conselleria de Educación, Cultura y Deporte with the Subvenciones para la contratación de personal investigador en fase postdoctoral, grant number APOSTD/2019/04, and by the Cooperativa Agrícola Sant Bernat Coop.V.García, L.; Parra-Boronat, L.; Basterrechea-Chertudi, DA.; Jimenez, JM.; Rocher-Morant, J.; Parra-Boronat, M.; García-Navas, JL.... (2019). Quantifying the Production of Fruit-Bearing Trees Using Image Processing Techniques. IARIA XPS Press. 14-19. http://hdl.handle.net/10251/180619S141

Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs

ABSTRACTObjective. To detect the presence of specific antibodies against Hepatitis E virus (HEV) in pigs slaughtered in Antioquia, the department where the greatest amount of pork is produced and consumed in Colombia. Materials and methods. Between September 2011 and May 2012, blood samples from pigs were obtained in five slaughterhouses of Antioquia, four of them located in the Aburrá Valley subregion and other located in northern subregión. Serum were evaluated with a commercial ELISA kit for diagnosing HEV in humans but adapted to detect IgG and IgM antibodies in pigs. Results. A 100.0% seropositivity for IgG antibodies was found in 1000 samples evaluated, and 82.06% for IgM antibodies were found in 740 samples. Conclusions. These results indicate that pigs in slaughter age in Antioquia, and possibly in Colombia, have been exposed to HEV at some point in their production process and a high percentage of them can arrive to slaughterhouses with recent infection.RESUMENObjetivo. Detectar la presencia de anticuerpos específicos contra el virus de la Hepatitis E (HEV) en cerdos faenados en Antioquia, departamento donde se produce y consume la mayor cantidad de carne de cerdo en Colombia. Materiales y métodos. Entre septiembre de 2011 y mayo de 2012, se obtuvieron muestras de sangre de cerdos en cinco plantas de faenado, cuatro de ellas ubicadas en el Valle de Aburrá y una en la subregión Norte del departamento de Antioquia, las cuales fueron evaluadas mediante un kit de ELISA comercial para diagnóstico de HEV en humanos pero adaptado para la detección de anticuerpos tipo IgG e IgM en cerdos. Resultados. Se encontró una seropositividad de 100.0% para anticuerpos tipo IgG en 1000 muestras evaluadas y de 82.06% para anticuerpos tipo IgM en 740 muestras. Una muestra de heces positiva para la detección del genoma HEV es similar al genotipo 3 encontrada en Estados Unidos. Conclusiones. Estos resultados indican que los cerdos en edad de faenado en Antioquia y posiblemente en Colombia, han tenido exposición a HEV del, un virus zoonótico emergente a nivel mundial, en algún momento de su proceso productivo

Spatial Pd-L1, Immune-Cell Microenvironment, and Genomic Copy-Number Alteration Patterns and Drivers of Invasive-Disease Transition in Prospective Oral Precancer Cohort

BACKGROUND: Studies of the immune landscape led to breakthrough trials of programmed death-1 (PD-1) inhibitors for recurrent/metastatic head and neck squamous cell carcinoma therapy. This study investigated the timing, influence of somatic copy-number alterations (SCNAs), and clinical implications of PD-L1 and immune-cell patterns in oral precancer (OPC). METHODS: The authors evaluated spatial CD3, CD3/8, and CD68 density (cells/mm RESULTS: Epithelial, but not CD68 immune-cell, PD-L1 expression was detected in 28% of OPCs, correlated with immune-cell infiltration, 9p21.3 loss of heterozygosity (LOH), and inferior oral cancer-free survival (OCFS), notably in OPCs with low CD3/8 cell density, dysplasia, and/or 9p21.3 LOH. High CD3/8 cell density in dysplastic lesions predicted better OCFS and eliminated the excess risk associated with prior oral cancer and dysplasia. PD-L1 and CD3/8 patterns revealed inferior OCFS in PD-L1 high intrinsic induction and dysplastic immune-cold subgroups. CONCLUSION: This report provides spatial insight into the immune landscape and drivers of OPCs, and a publicly available immunogenomic data set for future precancer interrogation. The data suggest that 9p21.3 LOH triggers an immune-hot inflammatory phenotype; whereas increased 9p deletion size encompassing CD274 at 9p24.1 may contribute to CD3/8 and PD-L1 depletion during invasive transition. The inferior OCFS in PD-L1-high, immune-cold OPCs support the development of T-cell recruitment strategies

Travel Demand Modeling Methodology Recommendations for the Link21 Program

This project aims to provide recommendations on the methodology and design specifications for the travel demand model to be built for the Link21 program in the Northern California megaregion. The Link21 program is a major rail investment program that will considerably improve and upgrade the passenger rail services in the Northern California megaregion, centered around the Transbay Corridor between Oakland and San Francisco in the San Francisco Bay Area

Design of a WSN for smart irrigation in citrus plots with fault-tolerance and energy-saving algorithms

[EN] Wireless sensor networks are widely used for monitoring different processes, including agriculture, in order to reach sustainability. One of the keys to sustainable crops is water saving. In particular, saving water is extremely important in arid and semiarid regions. In those regions, citrus trees are cultivated, and drip irrigation is used to save water. In this paper, we propose a smart irrigation system for citrus trees using a WSN. We describe the employed sensors and nodes for this proposal. Next, we present the proposed architecture and the operational algorithms for the nodes. Moreover, we designed different algorithms for fault tolerance and energy saving functionalities. The energy saving algorithm is based on the relevance of the gathered data, which is analyzed in order to consider whether the information should be forwarded or not. A TPC-based protocol is proposed to perform the communication among the nodes of our system. In addition, we present different simulations of the proposed system. Particularly, we show the consumed bandwidth and the remaining energy in the different nodes. Finally, we test different energy configurations to evaluate the network lifetime and the remaining energy when the first node depletes its energy.This work has been partially supported by the “Conselleria d' Educació, Investigació, Cultura i Esport” through the “Subvenciones para la contratación de personal investigator de carácter predoctoral (Convocatoria 2017)” Grant number ACIF/2017/069, by the “Ministerio de Educación, Cultura y Deporte”, through the “Ayudas para contratacion predoctoral de Formación del Profesorado Universitario FPU (Convocatoria 2014)”. Grant number FPU14/02953 and finally, the research leading to these results has received funding from “la Caixa” Foundation and Triptolemos Foundation. This work has also been partially supported by European Union through the ERANETMED (Euromediterranean Cooperation through ERANET joint activities and beyond) project ERANETMED3-227 SMARTWATIR.Parra-Boronat, L.; Rocher-Morant, J.; García-García, L.; Lloret, J.; Tomás Gironés, J.; Romero Martínez, JO.; Rodilla, M.... (2018). Design of a WSN for smart irrigation in citrus plots with fault-tolerance and energy-saving algorithms. Network Protocols and Algorithms. 10(2):95-115. https://doi.org/10.5296/npa.v10i2.13205S9511510

The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation.

OBJECTIVES: The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment. METHODS: The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms. RESULTS: Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed. CONCLUSIONS: mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force

Morphological variation in a secondary contact between divergent lineages of brown trout (Salmo trutta) from the Iberian Peninsula

The aim of this study was to analyze the morphological variation of brown trout (Salmo trutta) in the Duero basin, an Atlantic river basin in the Iberian Peninsula, where a spatial segregation of two divergent lineages was previously reported, based on isozyme, microsatellite and mtDNA data. In these studies, two divergent pure regions (Pisuerga and Lower-course) and several hybrid populations between them were identified. Morphological variation was evaluated in 11 populations representative of the genetic differentiation previously observed in the Duero basin, using multivariate analysis on 12 morphometric and 4 meristic traits. A large differentiation between populations was observed (interpopulation component of variance: 41.8%), similar to that previously detected with allozymes and microsatellites. Morphometric differentiation was also reflected by the high classification success of pure and hybrid individuals to their respective populations, using multivariate discriminant functions (94.1% and 79.0%, respectively). All multivariate and clustering analyses performed demonstrated a strong differentiation between the pure regions. The hybrid populations, though showing large differentiation among them, evidenced an intermediate position between the pure samples. Head and body shape traits were the most discriminant among the morphometric characters, while pectoral rays and gillrakers were the most discriminant among the meristic traits. These results confirmed the high divergence of the brown trout from the Duero basin and suggest some traits on which selection could be acting to explain the spatial segregation observed

Measurement of the cosmic ray spectrum above 4×10184{\times}10^{18} eV using inclined events detected with the Pierre Auger Observatory

A measurement of the cosmic-ray spectrum for energies exceeding $4{\times}10^{18}$ eV is presented, which is based on the analysis of showers with zenith angles greater than $60^{\circ}$ detected with the Pierre Auger Observatory between 1 January 2004 and 31 December 2013. The measured spectrum confirms a flux suppression at the highest energies. Above $5.3{\times}10^{18}$ eV, the "ankle", the flux can be described by a power law $E^{-\gamma}$ with index $\gamma=2.70 \pm 0.02 \,\text{(stat)} \pm 0.1\,\text{(sys)}$ followed by a smooth suppression region. For the energy ($E_\text{s}$) at which the spectral flux has fallen to one-half of its extrapolated value in the absence of suppression, we find $E_\text{s}=(5.12\pm0.25\,\text{(stat)}^{+1.0}_{-1.2}\,\text{(sys)}){\times}10^{19}$ eV.Comment: Replaced with published version. Added journal reference and DO