Sobre la variabilidad de Caprella acanthifera

Abstract not availabl

Los BRICS: Desarrollo, crecimiento y comercio a lo largo del siglo XXI.

Este trabajo se va a centrar principalmente en el análisis de varios indicadores decrecimiento, comercio y desarrollo general de los BRICS a lo largo del siglo XXI.Partiendo del origen de este grupo de cinco países (Brasil, Rusia, India, China ySudáfrica), se tratará de explicar el impulso de estas economías en vías de desarrollo ytambién de analizar factores como su PIB, población o superficie, y como últimoindicador, el índice de capital humano.Asimismo, se muestra un análisis de estos países para conocer en qué basan su economíay su comercio. También se examina la crisis de 2008, en qué medida afectó a este grupode países y qué repercusiones ha generado desde entonces. Pese a no disponer de muchosde los datos oficiales y seguir en expansión, es oportuno realizar un análisis conjunto a lacrisis de 2008 con la pandemia global de origen chino que estamos viviendo con elCOVID-19, más conocida como “coronavirus”.A lo largo del trabajo se explica cómo se comportan las economías emergentes antedichos problemas, así como también se ven expuestos algunos de los condicionantes másimportantes a nivel macroeconómico para ver la evolución en los países del grupo duranteel siglo: comenzando por los más prósperos y finalizando con los más desfavorecidos.Como conclusión general del estudio, queda reflejado cómo está cobrando vitalimportancia la capacidad de adaptación que deben tener los países ante los cambios en elentorno. Estas alteraciones de la normalidad como la crisis 2008 o el Covid, son algo casiimpredecible.De una manera objetiva se ponen en la misma balanza varios de los elementos que nospermitan conocer si los países del grupo BRICS tienen potencial para ser líderesmundiales en el siglo XXI.<br /

Sports-related lower limb muscle injuries: pattern recognition approach and MRI review

Muscle injuries of the lower limbs are currently the most common sport-related injuries, the impact of which is particularly significant in elite athletes. MRI is the imaging modality of choice in assessing acute muscle injuries and radiologists play a key role in the current scenario of multidisciplinary health care teams involved in the care of elite athletes with muscle injuries. Despite the frequency and clinical relevance of muscle injuries, there is still a lack of uniformity in the description, diagnosis, and classification of lesions. The characteristics of the connective tissues (distribution and thickness) differ among muscles, being of high variability in the lower limb. This variability is of great clinical importance in determining the prognosis of muscle injuries. Recently, three classification systems, the Munich consensus statement, the British Athletics Muscle Injury classification, and the FC Barcelona-Aspetar-Duke classification, have been proposed to assess the severity of muscle injuries. A protocolized approach to the evaluation of MRI findings is essential to accurately assess the severity of acute lesions and to evaluate the progression of reparative changes. Certain MRI findings which are seen during recovery may suggest muscle overload or adaptative changes and appear to be clinically useful for sport physicians and physiotherapists

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection

APPLYING ACTIVITY-BASED PROTEIN PROFILING TO IDENTIFY POTENTIALLY DRUGGABLE SERINE HYDROLASE ENZYMES IN LEISHMANIA SPP

Leishmaniasis is a poverty related and one of the most important tropical diseases in the world with more than 12 million infected people, 0.9 to 1.6 million new cases each year, between 20000 and 30000 deaths per year, and 350 million people at risk of infection. Available drugs face problems with toxicity, administration and resistance which challenge their effectiveness. Consequently, the identification of new drug targets and the development of new treatments are imperative. Although serine hydrolases have been demonstrated to participate in crucial roles in the life cycle of the parasite and its virulence, these have not been yet characterized and the Leishmania serinome remains surprisingly neglected. This project attempts map and explore therapeutic targets within SHs present in the Leishmania proteome using an activity-based protein profiling (ABPP) strategy in the quest to find new protein drug targets for drug discovery. Initial experiments using commercial fluorophosphonate (FPs) probes revealed significant differences between the SH expression levels throughout their life cycles and between different Leishmania spp. As these probes are only effective for in vitro labelling, a suite of cell permeable probes has been synthesized and applied to study the Leishmania serinome in whole cells. Following proteome labelling and enrichment, the mass spectrometry-based tagging method, iTRAQ, led to the identification of two serine proteases: Carboxypeptidase LmxM.18.0450 and prolyl oligopeptidase (POP) LmxM.36.6750. Using a competitive ABPP approach, we were able to identify small molecule inhibitors for these enzymes which did showed activity against both L. mexicana promastigotes and axenic amastigotes. Collectively, these findings suggest that the serinome is a valuable source of new drug targets and that ABPP is a reliable approach for target discovery

Diagnóstico general de la grippe ó influenza y de sus principales formas clínicas : tesis de doctorado

Tesis de doctorado leída en la Universidad Central, el 3 de julio de 1895Manuscrito firmadoTesis Doctorales históricasTesis Complutenses históricasmanuscritoFacultad de Medicinatruepu

Discovery of Leishmania Druggable Serine Proteases by Activity-Based Protein Profiling

Leishmaniasis are a group of diseases caused by parasitic protozoa of the genus Leishmania. Current treatments are limited by difficult administration, high cost, poor efficacy, toxicity, and growing resistance. New agents, with new mechanisms of action, are urgently needed to treat the disease. Although extensively studied in other organisms, serine proteases (SPs) have not been widely explored as antileishmanial drug targets. Herein, we report for the first time an activity-based protein profiling (ABPP) strategy to investigate new therapeutic targets within the SPs of the Leishmania parasites. Active-site directed fluorophosphonate probes (rhodamine and biotin-conjugated) were used for the detection and identification of active Leishmania serine hydrolases (SHs). Significant differences were observed in the SHs expression levels throughout the Leishmania life cycle and between different Leishmania species. Using iTRAQ-labelling-based quantitative proteomic mass spectrometry, we identified two targetable SPs in Leishmania mexicana: carboxypeptidase LmxM.18.0450 and prolyl oligopeptidase LmxM.36.6750. Druggability was ascertained by selective inhibition using the commercial serine protease inhibitors chymostatin, lactacystin and ZPP, which represent templates for future anti-leishmanial drug discovery programs. Collectively, the use of ABPP method complements existing genetic methods for target identification and validation in Leishmania

Knowledge exploitation from the web

Abstract. In the framework of Knowledge Management, the Internet can be a valuable source of information to produce new Knowledge. Here, an ontologybased web search system to ease the enterprise managers in the process of discovering new knowledge from the documents in the Internet is introduced. By means of a graphical user interface, the user of the system supplies an ontology in RDF to describe the domain of interest, and sets up some predefined parameters in order to constrain the search corpus. A distributed intelligent process works to achieve the levels of quality and quantity about the results that the user established. Several ideas about how to use this system and its application to seven real domains are also supplied.