PUFA content of silages prepared from tuna cannery wastes

Ensiling as one of the best methods for utilization of tuna wastes was investigated. docosahexaenoic acid [DHA C22:6(n-3)]- rich products were obtained from the wastes (viscera and dark meat) of four tuna species, namely longtail, skipjack, yellowfin and kawakawa by a procedure involving ensiling in organic acids followed by neutralization. Identification of fatty acids in the samples was performed by comparison with chromatograms of fatty acids standard. Total lipid content of silages varied from 10.41% in skipjack dark meat silage to 22.01% in kawakawa viscera silage, but all lipids contained high percentages of DHA and EPA [eicosapanteoneic acid C20:5(n-3)]. The highest DHA ratio (15% of total lipids) was found in the lipid of skipjack viscera silage and the highest ratio of EPA (11% of total lipids) belonged to the lipids of kawakawa dark meat silage. ANOVA test results indicated that DHA ratio was significantly high before silage preparation and linolenic acid [C18:3(n-3)] content significantly differed between the species (p<0.05)

Interferometric speckle visibility spectroscopy (ISVS) for human cerebral blood flow monitoring

Infrared light scattering methods have been developed and employed to non-invasively monitor human cerebral blood flow (CBF). However, the number of reflected photons that interact with the brain is low when detecting blood flow in deep tissue. To tackle this photon-starved problem, we present and demonstrate the idea of interferometric speckle visibility spectroscopy (ISVS). In ISVS, an interferometric detection scheme is used to boost the weak signal light. The blood flow dynamics are inferred from the speckle statistics of a single frame speckle pattern. We experimentally demonstrated the improvement of measurement fidelity by introducing interferometric detection when the signal photon number is insufficient. We apply the ISVS system to monitor the human CBF in situations where the light intensity is $\sim$100-fold less than that in common diffuse correlation spectroscopy (DCS) implementations. Due to the large number of pixels ($\sim 2\times 10^5$) used to capture light in the ISVS system, we are able to collect a similar number of photons within one exposure time as in normal DCS implementations. Our system operates at a sampling rate of 100 Hz. At the exposure time of 2 ms, the average signal photon electron number is $\sim$0.95 count/pixel, yielding a single pixel interferometric measurement signal-to-noise ratio (SNR) of $\sim$0.97. The total $\sim 2\times 10^5$ pixels provide an expected overall SNR of 436. We successfully demonstrate that the ISVS system is able to monitor the human brain pulsatile blood flow, as well as the blood flow change when a human subject is doing a breath holding task

Prevalence and risk factors of complication of endotracheal extubation in teaching hospitals affiliated with Jahrom University of medical science

Endotracheal intubation is to maintain a safe open airway to prevent pulmonary aspiration by administrating general anesthesia. Endotracheal tube, as a foreign body, can stimulate the patients’ airway during the emergence from general anesthesia and cause various reactions and complications immediately or within a multi-day delay.The present study intended to determine the prevalence and associated risk factors of the complications of endotracheal extubation (removal of endotracheal tube / ETT) within 24 hrs. since the surgery. To this end, a descriptive research was conducted on 200 adult candidates for elective and emergency surgery of endotracheal intubation by administrating general anesthesia. Data about the intended associated risk factors and complications were respectively collected in operating room (OR) and within 24 hrs. since surgery and were recorded in the questionnaire. The results indicated that the prevalent complications were sore throat (%21), cough (%12.5) and hoarseness (%15.5). There was not any case of dysphagia and bloody sputum (blood-streaked expectorant). Also, there was a significant relationship between sore throat and the type of surgery (P˂0.001). On the other hand, there was not any statistically significant relationship between sore throat and other associated risk factors (sex, age, weight, type of surgery and size of endotracheal tube). Likewise, not any significant relationship was observed between cough, hoarseness and the intended risk factors. To conclude, the present study found that the type of surgery has a significant effect on the incidence of sore throat within 24 hrs. since the surgical operation; thus, raising awareness of these risk factors and taking proper actions, particularly during intubation, can reduce the incidence of complications, in particular sore throat, and improve patients’ satisfaction.Keywords: General Anesthesia; Intubation; Complication

Fast Linear State Estimation for Unbalanced Distribution Systems Using Hybrid Measurements

Distribution system state estimation (DSSE) is traditionally solved iteratively using unsynchronized measurements provided by the SCADA system and/or smart meters. This article puts forward a decoupled linear state estimation (SE) method for unbalanced distribution systems. Contrary to conventional methods, the proposed linear DSSE (LDSSE) method can function with purely unsynchronized or hybrid synchronized/unsynchronized measurements. In the case of purely unsynchronized measurements, the voltage phase angles of the reference bus are acquired through local measurements. In the first stage, the proposed LDSSE method estimates the voltage phase angles in terms of network parameters and available measurements. These are referred to as pseudo-synchronized voltage phase angles, which establish a basis for deriving pseudo-synchronized voltage/current phasors. In the second stage, a set of linear equations are derived for each phase separately. Solving these equations results in estimates for voltage phasors. The linearity and decoupled nature of the proposed LDSSE method significantly reduces the computation time without impacting the accuracy of estimates. The superiority of the proposed LDSSE method over the existing methods is verified using extensive simulations conducted on several test feeders, delivering results 20 times faster than the nonlinear DSSE (NDSSE) on the 8500-bus test feeder

Planarian Cholinesterase: Molecular And Functional Characterization Of An Evolutionarily Ancient Enzyme To Study Organophosphorus Pesticide Toxicity

The asexual freshwater planarian Dugesia japonica has emerged as a medium-throughput alternative animal model for neurotoxicology. We have previously shown that D. japonica are sensitive to organophosphorus pesticides (OPs) and characterized the in vitro inhibition profile of planarian cholinesterase (DjChE) activity using irreversible and reversible inhibitors. We found that DjChE has intermediate features of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Here, we identify two candidate genes (Djche1 and Djche2) responsible for DjChE activity. Sequence alignment and structural homology modeling with representative vertebrate AChE and BChE sequences confirmed our structural predictions, and show that both DjChE enzymes have intermediate sized catalytic gorges and disrupted peripheral binding sites. Djche1 and Djche2 were both expressed in the planarian nervous system, as anticipated from previous activity staining, but with distinct expression profiles. To dissect how DjChE inhibition affects planarian behavior, we acutely inhibited DjChE activity by exposing animals to either an OP (diazinon) or carbamate (physostigmine) at 1 µM for 4 days. Both inhibitors delayed the reaction of planarians to heat stress. Simultaneous knockdown of both Djche genes by RNAi similarly resulted in a delayed heat stress response. Furthermore, chemical inhibition of DjChE activity increased the worms’ ability to adhere to a substrate. However, increased substrate adhesion was not observed in Djche1/Djche2 (RNAi) animals or in inhibitor-treated day 11 regenerates, suggesting this phenotype may be modulated by other mechanisms besides ChE inhibition. Together, our study characterizes DjChE expression and function, providing the basis for future studies in this system to dissect alternative mechanisms of OP toxicity

Reduced hospital stay, morphine consumption, and pain intensity with local infiltration analgesia after unicompartmental knee arthroplasty: A randomized double–blind study of 40 patients

Background and purpose The degree of postoperative pain is usually moderate to severe following knee arthroplasty. We investigated the efficacy of local administration of analgesics into the operating area, both intraoperatively and postoperatively

A Pilot Study on the Association of Lead, 8-Hydroxyguanine, and Malondialdehyde Levels in Opium Addicts’ Blood Serum with Illicit Drug Use and Non-Addict Persons

While a large body of literature has shown the health problems of illicit drug use, research is needed on how substance abuse impacts DNA damage and contaminants in blood, especially given Pb-contaminated opium. This pilot study aimed to evaluate the levels of lead (Pb), 8-hydroxy di-guanine (8-oxo-Gua), and malondialdehyde (MDA) in the blood serum of opium addicts and non-addict people. The current study is a case–control study with a cross-sectional design. A sample of 50 opium-addicted and non-addict adults were chosen for this study using convenience and random sampling methods. Participants were divided into two groups: addicts and non-addicts. The atomic absorption spectroscopy method was used to measure the quantity of Pb, and the Enzyme-Linked Immunosorbent Assay (ELISA) method was used to measure the amount of 8-oxo-Gua and MDA. The data were analyzed using an independent t-test. The results show that the amount of Pb in the blood serum of addicted women and men was higher than levels in non-addict men and women, for the study participants (p-value = 0.001). Blood levels were not significantly different between addicts and non-addicts for men or women for 8-oxo-Gua (p-value = 0.647 for women and p-value = 0.785 for men) and MDA (p-value = 0.867 for women and p-value = 0.995 for men). In general, addicts’ blood Pb levels were found to be substantially higher than those of normal non-addict persons in this pilot study. As a result, testing for blood Pb levels in addicts may be informative in instances when symptoms are inconclusive.</jats:p

Antiproliferative Withanolides from Datura wrightii

A new withanolide, named withawrightolide (1), and four known withanolides (2−5) were isolated from the aerial parts of Datura wrightii. The structure of compound 1 was elucidated through 2D NMR and other spectroscopic techniques. In addition, the structure of withametelin L (2) was confirmed by X-ray crystallographic analysis. Using MTS viability assays, withanolides 1−5 showed antiproliferative activities against human glioblastoma (U251 and U87), head and neck squamous cell carcinoma (MDA-1986), and normal fetal lung fibroblast (MRC-5) cells with IC50 values in the range between 0.56 and 5.6 μM