HIV and respiratory illness in the antiretroviral therapy era

Respiratory illness is a common manifestation of HIV infection. The availability of effective antiretroviral therapy (ART) has changed the pattern of respiratory ill-health experienced by people living with HIV (PLWH). Among populations with good access to ART, opportunistic respiratory infections such as Pneumocystis jirovecii pneumonia (PCP) are becoming less frequent. However, there is evidence to suggest that these populations may be at greater risk of serious non-AIDS illness including chronic respiratory disease. Although there is remaining uncertainty about the extent to which HIV represents an independent risk-factor for respiratory illness in individuals with a suppressed HIV viral load and immune reconstitution, in many settings PLWH have greater exposure to risk factors for respiratory illness (in particular tobacco smoking), which contribute to this burden of disease. As HIV-positive populations age, management of these conditions will therefore become increasingly important. Healthcare services need to manage this growing burden of chronic respiratory illness and provide access to preventative measures including smoking cessation and immunisation against vaccine-preventable respiratory infections in a way that is appropriate to the populations served

Defining the cultural milieu for implementing faculty practice in Pakistan

Faculty practice can promote a collaborative partnership mutually beneficial to both nursing education and service. However, little is known about its implementation in developing countries. The purpose of this study was to explore the potential for introducing faculty practice within the cultural milieu of Karachi, Pakistan. Focus groups of nursing faculty, staff and students were conducted in various settings: government, semi government and private institutions to elicit the data. Data analysis revealed that a more comprehensive definition of faculty practice is needed that will provide the foundation for a culturally acceptable model of faculty practice in Pakistan. Hierarchy dominates the current cultural milieu and must be addressed before faculty practice can be implemented

Natural scene statistics and the structure of orientation maps in the visual cortex

Visual activity after eye-opening influences feature map structure in primary visual cortex (V1). For instance, rearing cats in an environment of stripes of one orientation yields an over-representation of that orientation in V1. However, whether such changes also affect the higher-order statistics of orientation maps is unknown. A statistical bias of orientation maps in normally raised animals is that the probability of the angular difference in orientation preference between each pair of points in the cortex depends on the angle of the line joining those points relative to a fixed but arbitrary set of axes. Natural images show an analogous statistical bias; however, whether this drives the development of comparable structure in V1 is unknown. We examined these statistics for normal, stripe-reared and dark-reared cats, and found that the biases present were not consistently related to those present in the input, or to genetic relationships. We compared these results with two computational models of orientation map development, an analytical model and a Hebbian model. The analytical model failed to reproduce the experimentally observed statistics. In the Hebbian model, while orientation difference statistics could be strongly driven by the input, statistics similar to those seen in experimental maps arose only when symmetry breaking was allowed to occur spontaneously. These results suggest that these statistical biases of orientation maps arise primarily spontaneously, rather than being governed by either input statistics or genetic mechanisms

Cooked Rice and Oat Meal Similarly Reduce Hypercholesterolemia in Rats

Five groups of rats were given five different diets for 4 weeks after one week adaptation with standard casein. These diets include: casein diet as a control group (C), cooked rice ( R), cooked rice with 1% exogenous cholesterol (RC), oat meal (O), and oat meal with 1% exogenous cholesterol (OC) (Different analysis was determined on animals of these groups at the end of the experiment). The rats weights at the end of experiment showed that weight gain of control group rats was significantly higher (p.0.01) than other groups but there were no differences among other groups. The concentration of serum total cholesterol for (O) group (64.6 mg/100 ml) and that of (R) group (67.7 mg/100 ml) were significantly lower (p.0.01) than those of other groups and the control group was the highest one in all groups (112.42 mg/100 ml). The results also showed significant differences among mean relative weights of Liver, spleen and heart of different groups. The highest and lowest values of mean relative weight of rat's liver, spleen and heart in experimental groups were : (O) group (3.87) and R group (3.29) for liver, (R) group (0.48) and (O) group (0.38) for spleen, both RC and R groups (0.37) and (O) group (0.33) for heart respectively. The (R) and (O) diets significantly lowered the total fat content (24.0, 26.16 mg/kg respectively) while (O) diet lowered the cholesterol content (1.36 mg/kg) of the liver compared to the control diet (51.9 and 2.47 mg/kg for total fat and cholesterol respectively). For bile acid content in the feces, there were significant differences (p.0.01) among the different groups; (OC) group showed the highest (10.4 mg/gm of feces) and the control group showed the lowes

International standards for early fetal size and pregnancy dating based on ultrasound measurement of crown-rump length in the first trimester of pregnancy.

OBJECTIVES: There are no international standards for relating fetal crown-rump length (CRL) to gestational age (GA), and most existing charts have considerable methodological limitations. The INTERGROWTH-21(st) Project aimed to produce the first international standards for early fetal size and ultrasound dating of pregnancy based on CRL measurement. METHODS: Urban areas in eight geographically diverse countries that met strict eligibility criteria were selected for the prospective, population-based recruitment, between 9 + 0 and 13 + 6 weeks' gestation, of healthy well-nourished women with singleton pregnancies at low risk of fetal growth impairment. GA was calculated on the basis of a certain last menstrual period, regular menstrual cycle and lack of hormonal medication or breastfeeding in the preceding 2 months. CRL was measured using strict protocols and quality-control measures. All women were followed up throughout pregnancy until delivery and hospital discharge. Cases of neonatal and fetal death, severe pregnancy complications and congenital abnormalities were excluded from the study. RESULTS: A total of 4607 women were enrolled in the Fetal Growth Longitudinal Study, one of the three main components of the INTERGROWTH-21(st) Project, of whom 4321 had a live singleton birth in the absence of severe maternal conditions or congenital abnormalities detected by ultrasound or at birth. The CRL was measured in 56 women at < 9 + 0 weeks' gestation; these were excluded, resulting in 4265 women who contributed data to the final analysis. The mean CRL and SD increased with GA almost linearly, and their relationship to GA is given by the following two equations (in which GA is in days and CRL in mm): mean CRL = -50.6562 + (0.815118 × GA) + (0.00535302 × GA(2) ); and SD of CRL = -2.21626 + (0.0984894 × GA). GA estimation is carried out according to the two equations: GA = 40.9041 + (3.21585 × CRL(0.5) ) + (0.348956 × CRL); and SD of GA = 2.39102 + (0.0193474 × CRL). CONCLUSIONS: We have produced international prescriptive standards for early fetal linear size and ultrasound dating of pregnancy in the first trimester that can be used throughout the world

Magnetic activity and hot Jupiters of young Suns : the weak-line T Tauri stars V819 Tau and V830 Tau

SGG acknowledges support from the Science & Technology Facilities Council (STFC) via an Ernest Rutherford Fellowship [ST/J003255/1]. SHPA acknowledges financial support from CNPq, CAPES and Fapemig. AAV acknowledges support from the Swiss National Science Foundation (SNSF) via the allocation of an Ambizione Followship. Date of Acceptance: 06/08/2015We report results of a spectropolarimetric and photometric monitoring of the weak-line T Tauri stars (wTTSs) V819 Tau and V830 Tau within the MaTYSSE (Magnetic Topologies of Young Stars and the Survival of close-ingiant Exoplanets) programme, involving the ESPaDOnS spectropolarimeter at the Canada-France-Hawaii Telescope. At ≃3 Myr, both stars dissipated their discs recently and are interesting objects for probing star and planet formation. Profile distortions and Zeeman signatures are detected in the unpolarized and circularly polarized lines, whose rotational modulation we modelled using tomographic imaging, yielding brightness and magnetic maps for both stars. We find that the large-scale magnetic fields of V819 Tau and V830 Tau are mostly poloidal and can be approximated at large radii by 350-400 G dipoles tilted at≃30° to the rotation axis. They are significantly weaker than the field of GQ Lup, an accreting classical T Tauri star (cTTS) with similar mass and age which can be used to compare the magnetic properties of wTTSs and cTTSs. The reconstructed brightness maps of both stars include cool spots and warm plages. Surface differential rotation is small, typically ≃4.4 times smaller than on the Sun, in agreement with previous results on wTTSs. Using our Doppler images to model the activity jitter and filter it out from the radial velocity(RV) curves, we obtain RV residuals with dispersions of 0.033 and 0.104km s-1 for V819 Tau and V830 Tau, respectively. RV residuals suggest that a hot Jupiter may be orbiting V830 Tau, though additional data are needed to confirm this preliminary result. We find no evidence for close-in giant planet around V819 Tau.Publisher PDFPeer reviewe

Dual modality intravascular optical coherence tomography (OCT) and near-infrared fluorescence (NIRF) imaging: a fully automated algorithm for the distance-calibration of NIRF signal intensity for quantitative molecular imaging

Intravascular optical coherence tomography (IVOCT) is a well-established method for the high-resolution investigation of atherosclerosis in vivo. Intravascular near-infrared fluorescence (NIRF) imaging is a novel technique for the assessment of molecular processes associated with coronary artery disease. Integration of NIRF and IVOCT technology in a single catheter provides the capability to simultaneously obtain co-localized anatomical and molecular information from the artery wall. Since NIRF signal intensity attenuates as a function of imaging catheter distance to the vessel wall, the generation of quantitative NIRF data requires an accurate measurement of the vessel wall in IVOCT images. Given that dual modality, intravascular OCT–NIRF systems acquire data at a very high frame-rate (>100 frames/s), a high number of images per pullback need to be analyzed, making manual processing of OCT–NIRF data extremely time consuming. To overcome this limitation, we developed an algorithm for the automatic distance-correction of dual-modality OCT–NIRF images. We validated this method by comparing automatic to manual segmentation results in 180 in vivo images from six New Zealand White rabbit atherosclerotic after indocyanine-green injection. A high Dice similarity coefficient was found (0.97 ± 0.03) together with an average individual A-line error of 22 µm (i.e., approximately twice the axial resolution of IVOCT) and a processing time of 44 ms per image. In a similar manner, the algorithm was validated using 120 IVOCT clinical images from eight different in vivo pullbacks in human coronary arteries. The results suggest that the proposed algorithm enables fully automatic visualization of dual modality OCT–NIRF pullbacks, and provides an accurate and efficient calibration of NIRF data for quantification of the molecular agent in the atherosclerotic vessel wall.National Institutes of Health (U.S.) (NIH R01HL093717)Merck & Co., Inc

Correlation of Oncotype DX Recurrence Score with Histomorphology and Immunohistochemistry in over 500 Patients

Oncotype Dx is used to determine the recurrence risk (RR) in patients with estrogen receptor positive (ER+) and lymph node negative (LN−) breast cancer. The RR is divided into low (0-17), intermediat