238 research outputs found
Utility of Fine Resolution Land Cover Data for Modeling Northern Bobwhite Abundance in the Oaks and Prairies of Oklahoma
Management of northern bobwhite (Colinus virginianus) requires landscape-level planning to promote sustainable populations. Limitations in time and resources necessitate the use of large geographic datasets to efficiently evaluate habitat suitability across landscapes. Many such datasets, however, are limited by a lack of detailed and current information relevant to regional management efforts. To meet this need, regional partners recently released the Oklahoma Ecological Systems Mapping (OESM) product, which offers high spatial and thematic resolution vegetation data, current to 2015. We evaluated the utility of the new OESM product for modelling abundance of northern bobwhite and other grassland birds, relating percent cover types to bird survey data from the Oaks and Prairies Joint Venture. Using an information-theoretic approach (AIC), we compared the performance of OESM to the more widely known National Land Cover Dataset (NLCD). The OESM data provided information on 20 land cover types at 10-m resolution compared 7 types and 30-m resolution for NLCD. We conducted a total of 2,367 individual counts for breeding birds from May–July, 2014–2016, across 10 counties in the Oklahoma Oaks and Prairies ecoregion. We used generalized linear mixed models to control for random effects of year and AICc to evaluate model performance. OESM models vastly outperformed NLCD for 6/7 species (AIC weights \u3e0.99). Northern bobwhite was the only species where NLCD was the highest performing model. These results suggest that, in contrast to other grassland species, added levels of habitat complexity do not inform our ability to model local bobwhite abundance
Software systems for operation, control, and monitoring of the EBEX instrument
We present the hardware and software systems implementing autonomous
operation, distributed real-time monitoring, and control for the EBEX
instrument. EBEX is a NASA-funded balloon-borne microwave polarimeter designed
for a 14 day Antarctic flight that circumnavigates the pole. To meet its
science goals the EBEX instrument autonomously executes several tasks in
parallel: it collects attitude data and maintains pointing control in order to
adhere to an observing schedule; tunes and operates up to 1920 TES bolometers
and 120 SQUID amplifiers controlled by as many as 30 embedded computers;
coordinates and dispatches jobs across an onboard computer network to manage
this detector readout system; logs over 3~GiB/hour of science and housekeeping
data to an onboard disk storage array; responds to a variety of commands and
exogenous events; and downlinks multiple heterogeneous data streams
representing a selected subset of the total logged data. Most of the systems
implementing these functions have been tested during a recent engineering
flight of the payload, and have proven to meet the target requirements. The
EBEX ground segment couples uplink and downlink hardware to a client-server
software stack, enabling real-time monitoring and command responsibility to be
distributed across the public internet or other standard computer networks.
Using the emerging dirfile standard as a uniform intermediate data format, a
variety of front end programs provide access to different components and views
of the downlinked data products. This distributed architecture was demonstrated
operating across multiple widely dispersed sites prior to and during the EBEX
engineering flight.Comment: 11 pages, to appear in Proceedings of SPIE Astronomical Telescopes
and Instrumentation 2010; adjusted metadata for arXiv submissio
EBEX: A balloon-borne CMB polarization experiment
EBEX is a NASA-funded balloon-borne experiment designed to measure the
polarization of the cosmic microwave background (CMB). Observations will be
made using 1432 transition edge sensor (TES) bolometric detectors read out with
frequency multiplexed SQuIDs. EBEX will observe in three frequency bands
centered at 150, 250, and 410 GHz, with 768, 384, and 280 detectors in each
band, respectively. This broad frequency coverage is designed to provide
valuable information about polarized foreground signals from dust. The
polarized sky signals will be modulated with an achromatic half wave plate
(AHWP) rotating on a superconducting magnetic bearing (SMB) and analyzed with a
fixed wire grid polarizer. EBEX will observe a patch covering ~1% of the sky
with 8' resolution, allowing for observation of the angular power spectrum from
\ell = 20 to 1000. This will allow EBEX to search for both the primordial
B-mode signal predicted by inflation and the anticipated lensing B-mode signal.
Calculations to predict EBEX constraints on r using expected noise levels show
that, for a likelihood centered around zero and with negligible foregrounds,
99% of the area falls below r = 0.035. This value increases by a factor of 1.6
after a process of foreground subtraction. This estimate does not include
systematic uncertainties. An engineering flight was launched in June, 2009,
from Ft. Sumner, NM, and the long duration science flight in Antarctica is
planned for 2011. These proceedings describe the EBEX instrument and the North
American engineering flight.Comment: 12 pages, 9 figures, Conference proceedings for SPIE Millimeter,
Submillimeter, and Far-Infrared Detectors and Instrumentation for Astronomy V
(2010
Detection and Identification of Old World Leishmania by High Resolution Melt Analysis
Protozoal parasites of the genus Leishmania are transmitted by sand fly bites to humans and animals. Three major forms of disease are caused by these parasites: cutaneous leishmaniasis, responsible for disfiguring skin wounds; mucocutaneous leishmaniasis, causing non-healing ulceration around the mouth and nose; and the potentially fatal visceral leishmaniasis, involving internal organs such as the spleen and liver. More than 2 million new human infections are caused annually by leishmaniasis globally, it is endemic in more than 88 countries and prevalent also as an imported disease in non-endemic regions due to travel and tourism. Most species of Leishmania that infect humans are zoonotic and transmitted from animal reservoir hosts. As various leishmanial parasites cause disease with similar symptoms, but require different therapeutic regimens and have dissimilar prognoses, reliable, sensitive and rapid diagnostic assays are needed. This study focuses on the five main species that cause leishmaniasis in the Old World. It presents a new assay for rapid detection, species identification and quantification of leishmanial parasites in clinical samples, reservoir hosts and sand flies. This technique could be especially valuable in regions where several leishmanial species exist, in non-endemic regions where infected patients require a rapid diagnosis, and for epidemiological host and vector studies leading to prevention programs
Observing the Evolution of the Universe
How did the universe evolve? The fine angular scale (l>1000) temperature and
polarization anisotropies in the CMB are a Rosetta stone for understanding the
evolution of the universe. Through detailed measurements one may address
everything from the physics of the birth of the universe to the history of star
formation and the process by which galaxies formed. One may in addition track
the evolution of the dark energy and discover the net neutrino mass.
We are at the dawn of a new era in which hundreds of square degrees of sky
can be mapped with arcminute resolution and sensitivities measured in
microKelvin. Acquiring these data requires the use of special purpose
telescopes such as the Atacama Cosmology Telescope (ACT), located in Chile, and
the South Pole Telescope (SPT). These new telescopes are outfitted with a new
generation of custom mm-wave kilo-pixel arrays. Additional instruments are in
the planning stages.Comment: Science White Paper submitted to the US Astro2010 Decadal Survey.
Full list of 177 author available at http://cmbpol.uchicago.ed
The Signature of Galactic Tides in Local Group Dwarf Spheroidals
(Abridged) We use N-body simulations to explore the effects of tidal
stripping on the structure of dwarf spheroidal galaxies (dSphs). We model dSphs
as King spheres embedded in NFW dark halos that orbit the Galactic potential on
eccentric orbits. We find that episodes of stellar mass loss remove the initial
cutoff of the bound stellar core. Once equilibrium has been re-established the
outer mass profile approaches a power-law in projection that is well described
by a simple Plummer model. Tides also result in transient features in the outer
density profile. As the system re-equilibrates, an outward-moving "excess" of
stars is found at radii where the local crossing time exceeds the time elapsed
since pericenter. If the orbit is known, these results provide a simple way to
assess whether "breaks" and "bumps" in the profile of dSphs are actually tidal
in origin. We apply this to the Sagittarius dwarf and, encouragingly, identify
two features in the profile that may be traced to its two last pericentric
passages. Applied to Leo I, we find that any tidal break would locate beyond
the area surveyed by current data, casting doubt on recent claims of the
detection of tidal debris around this galaxy. For Carina, the tidal break
should occur at a radius twice farther than observed, suggesting that the outer
excess of stars is not tidal in origin unless its orbit is in error. A similar
comment applies to Sculptor, whose pericenter appears too large for Galactic
tides to be important but whose outer profile, like that of Draco, nonetheless
follows closely a Plummer-law. Fornax and Leo II show no sign of a power-law
outer profile, suggesting that they have not suffered stellar tidal stripping.
Published profiles for other, fainter Milky Way dSph companions do not extend
sufficiently far to allow for conclusive assessment.Comment: 14 pages, ApJ accepte
MicroRNA-21 Exhibits Antiangiogenic Function by Targeting RhoB Expression in Endothelial Cells
BACKGROUND: MicroRNAs (miRNAs) are endogenously expressed small non-coding RNAs that regulate gene expression at post-transcriptional level. The recent discovery of the involvement of these RNAs in the control of angiogenesis renders them very attractive in the development of new approaches for restoring the angiogenic balance. Whereas miRNA-21 has been demonstrated to be highly expressed in endothelial cells, the potential function of this miRNA in angiogenesis has never been investigated. METHODOLOGY/PRINCIPAL FINDINGS: We first observed in endothelial cells a negative regulation of miR-21 expression by serum and bFGF, two pro-angiogenic factors. Then using in vitro angiogenic assays, we observed that miR-21 acts as a negative modulator of angiogenesis. miR-21 overexpression reduced endothelial cell proliferation, migration and the ability of these cells to form tubes whereas miR-21 inhibition using a LNA-anti-miR led to opposite effects. Expression of miR-21 in endothelial cells also led to a reduction in the organization of actin into stress fibers, which may explain the decrease in cell migration. Further mechanistic studies showed that miR-21 targets RhoB, as revealed by a decrease in RhoB expression and activity in miR-21 overexpressing cells. RhoB silencing impairs endothelial cell migration and tubulogenesis, thus providing a possible mechanism for miR-21 to inhibit angiogenesis. Finally, the therapeutic potential of miR-21 as an angiogenesis inhibitor was demonstrated in vivo in a mouse model of choroidal neovascularization. CONCLUSIONS/SIGNIFICANCE: Our results identify miR-21 as a new angiogenesis inhibitor and suggest that inhibition of cell migration and tubulogenesis is mediated through repression of RhoB
Optimization of Topical Therapy for Leishmania major Localized Cutaneous Leishmaniasis Using a Reliable C57BL/6 Model
When initiating the cutaneous disease named cutaneous leishmaniasis (CL), Leishmania parasites develop within the parasitophorous vacuoles of phagocytes residing in and/or recruited to the dermis, a process leading to more or less chronic dermis and epidermis-damaging inflammatory processes. Topical treatment of CL could be a mainstay in its management. Any improvements of topicals, such as new vehicles and shorter optimal contact regimes, could facilitate their use as an ambulatory treatment. Recently, WR279396, a third-generation aminoglycoside ointment, was designed with the aim to provide stability and optimal bioavailability for the molecules expected to target intracellular Leishmania. Two endpoints were expected to be reached: i) accelerated clearance of the maximal number of parasites, and ii) accelerated and stable repair processes without scars. A mouse model of CL was designed: it relies on the intradermal inoculation of luciferase-expressing Leishmania, allowing for in vivo bioluminescence imaging of the parasite load fluctuation, which can then be quantified simultaneously with the onset and resolution of clinical signs. These quantitative readout assays, deployed in real time, provide robust methods to rapidly assess efficacy of drugs/compounds i) to screen treatment modalities and ii) allow standardized comparison of different therapeutic agents
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