1,843 research outputs found
Distorted HI Gas in the Widely Separated LIRG Arp 256
We present new interferometric HI and CO (1-0) observations of the luminous
infrared source, Arp 256. Arp 256 consists of two spiral galaxies in an early
stage of merging, with a projected nuclear separation of 29 kpc (54") and an
infrared luminosity of 2.0E11 L_sun. Despite the large separation of the
galaxies' nuclei and mildly disrupted stellar components, the HI disks are
found to be strongly disrupted, and the southern galaxy in Arp 256 shows an
elevated star formation efficiency, which is consistent with a nuclear
starburst. Both of these results run contrary to expectations, posing
interesting questions on the physical mechanisms involved in stimulating star
formation during an interaction.Comment: 19 pages, 7 figures. Accepted for publication in AJ. Author added.
Full resolution figures available at
http://astro.uchicago.edu/home/web/jchen/arp25
Minkowski's Object: A Starburst Triggered by a Radio Jet, Revisited
We present neutral hydrogen, ultraviolet, optical and near-infrared imaging,
and optical spectroscopy, of Minkowski's Object (MO), a star forming peculiar
galaxy near NGC 541. The observations strengthen evidence that star formation
in MO was triggered by the radio jet from NGC 541. Key new results are the
discovery of a 4.9E8 solar mass double HI cloud straddling the radio jet
downstream from MO, where the jet changes direction and decollimates; strong
detections of MO, also showing double structure, in UV and H-alpha; and
numerous HII regions and associated clusters in MO. In UV, MO resembles the
radio-aligned, rest-frame UV morphologies in many high redshift radio galaxies
(HzRGs), also thought to be caused by jet-induced star formation. MO's stellar
population is dominated by a 7.5 Myr-old, 1.9E7 solar mass instantaneous burst,
with current star formation rate 0.52 solar masses per year (concentrated
upstream from where the HI column density is high). This is unlike the
jet-induced star formation in Centaurus A, where the jet interacts with
pre-existing cold gas; in MO the HI may have cooled out of a warmer, clumpy
intergalactic or interstellar medium as a result of jet interaction, followed
by collapse of the cooling clouds and subsequent star formation (consistent
with numerical simulations). Since the radio source that triggered star
formation in MO is much less luminous, and therefore more common, than powerful
HzRGs, and because the environment around MO is not particularly special in
terms of abundant dense, cold gas, jet-induced star formation in the early
universe might be even more prevalent than previously thought.Comment: 52 pages, 15 figures, accepted for publication in Ap
Lipooligosaccharide Structure is an Important Determinant in the Resistance of Neisseria Gonorrhoeae to Antimicrobial Agents of Innate Host Defense
The strict human pathogen Neisseria gonorrhoeae has caused the sexually transmitted infection termed gonorrhea for thousands of years. Over the millennia, the gonococcus has likely evolved mechanisms to evade host defense systems that operate on the genital mucosal surfaces in both males and females. Past research has shown that the presence or modification of certain cell envelope structures can significantly impact levels of gonococcal susceptibility to host-derived antimicrobial compounds that bathe genital mucosal surfaces and participate in innate host defense against invading pathogens. In order to facilitate the identification of gonococcal genes that are important in determining levels of bacterial susceptibility to mediators of innate host defense, we used the Himar I mariner in vitro mutagenesis system to construct a transposon insertion library in strain F62. As proof of principle that this strategy would be suitable for this purpose, we screened the library for mutants expressing decreased susceptibility to the bacteriolytic action of normal human serum (NHS). We found that a transposon insertion in the lgtD gene, which encodes an N-acetylgalactosamine transferase involved in the extension of the α-chain of lipooligosaccharide (LOS), could confer decreased susceptibility of strain F62 to complement-mediated killing by NHS. By complementation and chemical analyses, we demonstrated both linkage of the transposon insertion to the NHS-resistance phenotype and chemical changes in LOS structure that resulted from loss of LgtD production. Further truncation of the LOS α-chain or loss of phosphoethanolamine (PEA) from the lipid A region of LOS also impacted levels of NHS-resistance. PEA decoration of lipid A also increased gonococcal resistance to the model cationic antimicrobial polymyxin B. Taken together, we conclude that the Himar I mariner in vitro mutagenesis procedure can facilitate studies on structures involved in gonococcal pathogenesis
Uma sondagem via internet projetada para avaliar a vida diária dos idosos
O declínio da saúde física e do funcionamento cognitivo, em decorrência da idade, pode ter efeitos profundos na vida diária dos idosos, inclusive no desempenho de tarefas de autocuidado e na participação em atividades sociais. No entanto, pouco ainda se sabe sobre essa relação, devido à falta de medidas adequadas para uma avaliação diária dessa população. Este artigo apresenta um projeto piloto interdisciplinar que desenvolveu medidas diárias de saúde, funcionamento físico, atividades de autocuidado e atividades sociais entre idosos. No estudo, 182 idosos (com idades entre 60 e 90 anos) participaram, através da internet, de uma breve (~15 minutos) pesquisa de base, e de avaliações diárias, durante sete dias consecutivos. As medidas incluídas nesse estudo, e suas estatísticas descritivas, são apresentadas neste trabalho. Na conclusão são discutidas suas implicações para pesquisas futuras, incluindo os desafios envolvidos no uso da internet para a coleta de dados de idosos
Structure of a Bovine Thrombin-Hirudin\u3csub\u3e51-65\u3c/sub\u3e Complex Determined by a Combination of Molecular Replacement and Graphics. Incorporation of Known Structural Information in Molecular Replacement
Crystals of the bovine thrombin-hirudins51-65 complex have space group P6122 with cell constants a = 116.4, and c = 200.6 Å and two thrombin molecules in the asymmetric unit. Only one thrombin molecule could be located by generalized molecular replacement; the second was fit visually as a rigid body to an improved electron-density difference map. The structure was refined to R = 0.192 with two B values per residue (main chain and side chain) at 3.2 Å. The polar interactions of the peptides with the exosite of thrombin show differences consistent with the known flexibility in the interactions of the C-terminal peptide of hirudin with thrombin. The hirudin peptide in complex 2 has a higher temperature factor as compared with peptide 1 which may be correlated partly with a larger number of short-range electrostatic interactions between peptide 1 and thrombin and partly with the fact that thrombin 2 is -thrombin which is cleaved at Thr149A near the peptide binding site. Later, using this structure as a test case, it was shown that the position for the second thrombin could also be determined by a novel modification of the molecular-replacement method in which the contribution of the known molecule is subtracted from the structure factors. This approach is facile and applicable to any crystal containing two or more macromolecules in the asymmetric unit in which some but not all of the molecules can be determined by molecular replacement
Genome-wide gene by environment study of time spent in daylight and chronotype identifies emerging genetic architecture underlying light sensitivity
Study Objectives: Light is the primary stimulus for synchronizing the circadian clock in humans. There are very large interindividual differences in the sensitivity of the circadian clock to light. Little is currently known about the genetic basis for these interindividual differences.Methods: We performed a genome-wide gene-by-environment interaction study (GWIS) in 280 897 individuals from the UK Biobank cohort to identify genetic variants that moderate the effect of daytime light exposure on chronotype (individual time of day preference), acting as “light sensitivity” variants for the impact of daylight on the circadian system.Results: We identified a genome-wide significant SNP mapped to the ARL14EP gene (rs3847634; p < 5 × 10−8), where additional minor alleles were found to enhance the morningness effect of daytime light exposure (βGxE = −.03, SE = 0.005) and were associated with increased gene ARL14EP expression in brain and retinal tissues. Gene-property analysis showed light sensitivity loci were enriched for genes in the G protein-coupled glutamate receptor signaling pathway and genes expressed in Per2+ hypothalamic neurons. Linkage disequilibrium score regression identified Bonferroni significant genetic correlations of greater light sensitivity GWIS with later chronotype and shorter sleep duration. Greater light sensitivity was nominally genetically correlated with insomnia symptoms and risk for post-traumatic stress disorder (PTSD).Conclusions: This study is the first to assess light as an important exposure in the genomics of chronotype and is a critical first step in uncovering the genetic architecture of human circadian light sensitivity and its links to sleep and mental healt
Crystallization of Hemoglobins II and III of the Symbiont-Harboring Clam Lucina pectinata
Diffraction data to 2.7 A resolution were measured on crystals of the homotetramers of components II and III of the cytoplacmic hemoglobin of the symbiont-harboring clam Lucina pectinata. Even though the crystallization conditions are different and the sequence homology of the two hemoglobins is only 63%, the crystals are isomorphous to each other and to the heterotetramer Hb II/III, implying that the residues primarily involved in the intermolecular interactions and responsible for crystal cohesion may be invariant
Full-Shell X-Ray Optics Development at NASA Marshall Space Flight Center
NASAs Marshall Space Flight Center (MSFC) maintains an active research program toward the development of high-resolution, lightweight, grazing-incidence x-ray optics to serve the needs of future x-ray astronomy missions such as Lynx. MSFC development efforts include both direct fabrication (diamond turning and deterministic computer-controlled polishing) of mirror shells and replication of mirror shells (from figured, polished mandrels). Both techniques produce full-circumference monolithic (primary + secondary) shells that share the advantages of inherent stability, ease of assembly, and low production cost. However, to achieve high-angular resolution, MSFC is exploring significant technology advances needed to control sources of figure error including fabrication- and coating-induced stresses and mounting-induced distortions
Peri- and postnatal effects of prenatal adenoviral VEGF gene therapy in growth-restricted sheep
Supported by Wellcome Trust project grant 088208 to A.L.D., J.M.W., D.M.P., I.C.Z., and J.F.M. Wellbeing of Women research training fellowship 318 to D.J.C., Scottish Government work package 4.2 to J.M.W., J.S.M., and R.P.A., as well as funding from the National Institute for Health Research University College London Hospitals Biomedical Research Centre A.L.D. and D.M.P., the British Heart Foundation to I.C.Z., and Ark Therapeutics Oy, Kuopio, Finland, which supplied adenovirus vectors free of charge.Peer reviewedPublisher PD
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