The Prevalence of Carbon-13 in Respiratory Carbon Dioxide As an Indicator of the Type of Endogenous Substrate. The change from lipid to carbohydrate during the respiratory rise in potato slices

Isotope discrimination is a common feature of biosynthesis in nature, with the result that different classes of carbon compounds frequently display different 13C/12C ratios. The 13C/12C ratio of lipid in potato tuber tissue is considerably lower than that for starch or protein. We have collected respiratory CO2 from potato discs in successive periods through 24 hr from the time of cutting—an interval in which the respiration rate rises 3–5-fold. The 13C/12C ratio of the evolved CO2 was determined for each period, and compared with the 13C/12C ratios of the major tissue metabolites. In the first hours the carbon isotope ratio of the CO2 matches that of lipid. With time, the ratio approaches that typical of starch or protein. An estimation has been made of the contribution of lipid and carbohydrate to the total respiration at each juncture. In connection with additional observations, it was deduced that the basal, or initial, respiration represents lipid metabolism —- possibly the alpha-oxidation of long chain fatty acids -— while the developed repiration represents conventional tricarboxylic acid cycle oxidation of the products of carbohydrate glycolysis. The true isotopic composition of the respiratory CO2 may be obscured by fractionation attending the refixation of CO2 during respiration, and by CO2 arising from dissolved CO2 and bicarbonate preexisting in the tuber. Means are described for coping with both pitfalls

Operating limits for acoustic measurement of rolling bearing oil film thickness

An ultrasonic pulse striking a thin layer of liquid trapped between solid bodies will be partially reflected. The proportion reflected is a function of the layer stiffness, which in turn depends on the film thickness and its bulk modulus. In this work, measurements of reflection have been used to determine the thickness of oil films in elastohydrodynamic lubricated (EHL) contacts. A very thin liquid layer behaves like a spring when struck by an ultrasonic pulse. A simple quasi-static spring model can be used to determine the proportion of the ultrasonic waves reflected. Experiments have been performed on a model EHL contact between a ball and a flat surface. A transducer is mounted above the contact such that the ultrasonic wave is focused onto the oil film. The reflected signals are captured and passed to a PC for processing. Fourier analysis gives the reflection spectrum that is then used to determine the stiffness of the liquid layer and hence its thickness. In further testing, an ultrasonic transducer has been mounted in the housing of a deep-groove ball bearing to measure the film generated at the outer raceway as each ball passes. Results from both the ball-flat and ball bearing measurements agree well with steady-state theoretical EHL predictions. The limits of the measuring technique, in terms of the measurable rolling bearing size and operating parameters, have been investigated

Acoustic measurement of lubricant-film thickness distribution in ball bearings

An oil-film thickness monitoring system capable of providing an early warning of lubrication failure in rolling element bearings has been developed. The system is used to measure the lubricant-film thickness in a conventional deep groove ball bearing (shaft diameter 80 mm, ball diameter 12.7 mm). The measurement system comprises a 50 MHz broadband ultrasonic focused transducer mounted on the static outer raceway of the bearing. Typically the lubricant-films in rolling element bearings are between 0.1-1.0 μm in thickness and so are significantly smaller than the ultrasonic wavelength. A quasistatic spring model is used to calculate oil-film thickness from the measured reflection coefficient data. An accurate triggering system has been developed to enable multiple reflection coefficient measurements to be made as the contact ellipse sweeps over the measurement location. Experiments are described in which the loading conditions and rotational speed are varied. Lubricant-film thickness distributions measured ultrasonically are described and are shown to agree well with the predictions from classical elastohydrodynamic (EHD) lubrication theory, particularly at high radial loads and low rotary speeds. A range of parameters affecting the performance of the measurement are discussed and the limits of operation of the measurement technique defined. © 2006 Acoustical Society of America

Monitoring of lubricant film failure in a ball bearing using ultrasound

A lubricant-film monitoring system for a conventional deep groove ball bearing (type 6016, shaft diameter 80 mm, ball diameter 12.7 mm) is described. A high-firequency (50 MHz) ultrasonic transducer is mounted on the static outer raceway of the bearing. The transducer is focused on the ball-raceway interface and used to measure the reflection coefficient of the lubricant in the "contact" ellipse between bearing components. The reflection coefficient characterizes the lubricant film and can be used to calculate its thickness. An accurate triggering system enables multiple reflection measurements to be made as each lubricated contact moves past the measurement location. Experiments are described in which bearings were deliberately caused to fail by the addition of acetone, water and sand to the lubricant. The ultrasonic reflection coefficient was monitored as a function of time as the failure occurred. Also monitored were the more standard parameters, temperature and vibration. The results indicate that the ultrasonic measurements are able to detect the failures before seizure. It is also observed that, when us,ed in parallel, these monitoring techniques offer the potential to diagnose the failure mechanism and hence improve predictions of remaining life

Toxoplasma gondii is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: A prospective cohort study

Background Toxoplasma gondii causes reproductive losses in sheep worldwide, including Australia. The reproductive performance of primiparous ewes is typically lower than for mature, multiparous ewes, and younger ewes are more likely to be immunologically naïve and therefore more susceptible to reproductive disease if T. gondii infection occurs during pregnancy. The aim of this study was to assess the impact of infection with T. gondii on the reproductive performance of primiparous ewes in southern Australia using a prospective cohort study. This will inform the need for targeted control strategies for T. gondii in Australian sheep. Results Toxoplasma gondii seropositivity using indirect ELISA was detected at 16/28 farms located across southern Australia. Apparent seropositivity to T. gondii was lower in primiparous ewes (1.1, 95% confidence interval (CI) 0.6, 1.8) compared to mature, multiparous ewes (8.1, 95% CI 6.0, 10.5; P < 0.001). Toxoplasma gondii seroconversion during the gestation and lambing period was confirmed for 11/1097 (1.0, 95% CI 0.5, 1.7) of pregnant primiparous ewes that failed to raise a lamb, and 1/161 (0.6, 95% CI 0.1, 2.9) primiparous ewes with confirmed mid-pregnancy abortion. Conclusions Low frequency of detection of T. gondii seroconversion during gestation and low frequency of seropositivity to T. gondii suggests that toxoplasmosis was not an important contributor to reproductive losses in primiparous ewes on farms located over a wide geographical area in southern Australia

Lessons from America: teaching politics with the Google generation

The superabundance of information available, particularly through the internet, is posing many challenges to the traditional pedagogy of higher education. Much of this concern is focused on the ubiquity of the search engine Google, with Tara Brabazon amongst the most conspicuous to claim that ‘the popularity of Google is facilitating laziness, poor scholarship and compliant thinking’ (Brabazon, 2007: 15). At the very least, it is clear that Google – as well as the more specialist Google Scholar and the (mostly) open-edited online encyclopedia Wikipedia – have quickly established prominent positions in many students’ strategies to locate information for various assignments. This has led to particular cohorts of students being dubbed, often in a derogatory fashion, the Google generation. While making it clear that many of the stereotypical claims made on behalf of this group are unfounded, this paper will present evidence to support some of the concerns made by Brabazon and others. In addition, it will define the concept that many have recognised as the potential solution to this problem, and will examine one ambitious attempt from the US to confront these problems directly. Moreover, it will be argued that adoption of similar strategies in the UK might address some important criticisms levelled at general university-level politics education in this country

Peeling back the layers: Deconstructing information literacy discourse in higher education

The discourses of information literacy practice create epistemological assumptions about how the practice should happen, who should be responsible and under what conditions instruction should be given. Analysis of a wide range of documents and texts emerging from the Higher Education (HE) sector suggest that information literacy (IL) is shaped by two competing and incongruent narratives. The outward facing narrative of information literacy (located in information literacy standards and guidelines) positions information literacy as an empowering practice that arms students with the knowledge and skills to battle the complexity of the modern information world. In contrast, the inward facing narrative (located in information literacy texts) positions students as lacking appropriate knowledge, skills and agency. This deficit perception, which has the capacity to influence pedagogical practice, is at odds with constructivist and action-oriented views that are espoused within information literacy instructional pedagogy. This presentation represents the first paper in a research programme that interrogates the epistemological premises and discourses of information literacy within HE