Efecto de la cocción sobre algunas características nutricionales del frijol

Se estudiaron siete variedades de frijol de los tipos: Flor de Mayo, Negro, Bayo y Canario, además de la línea BAT 104. El objetivo del presente estudio fue analizar las características tecnológicas y nutricionales, así como los cambios que por efecto de la cocción resultan en la digestibilidad de la proteína y la actividad del inhibidor de tripsina en el grano de los materiales. Se encontraron diferencias significativas entre variedades en el peso, tamaño, porcentaje de testa, tiempo de cocción, actividad del inhibidor de tripsina y digestibilidad. El contenido de proteína en los materiales varió de 22.6 a 27.3 %; asimismo se detectaron diferencias entre los materiales en su concentración de componentes químicos, tales como almidón, fibra cruda, cenizas, lisina y triptofano. Una baja actividad del inhibidor de tripsina en el frijol cocido estuvo mas correlacionada con un mayor tiempo de cocción y menor tamaño de grano que con el contenido del mismo inhibidor en el frijol crudo. La digestibilidad de los materiales se incrementó en promedio 10% como consecuencia de la cocción

Caracterización de cuatro variedades de frijol (Phaseolus vulgaris L.) por sus cualidades físicas y nutricionales. I. Aspectos metodológicos.

La semilla de las variedades de frijol: BAT 104, Bayo Río Grande, Canario 107 y Jamapa se evaluaron con el objetivo de caracterizarla mediante el análisis del contenido de proteína, L-triptofano (L-trp) y L-metionina disponible (L-met), así como sus cualidades tecnológicas. Se realizaron pruebas preliminares sobre contenido de L-met y L-trp en proteína soluble en extractos acuosos de NaCl, ácido acético y etanol empleando los métodos microbiológico y espectrofotométrico, respectivamente. Bayo Río Grande, la de mayor tiempo de cocción (98 minutos), mostró la mayor proporción en peso de testa (11,4); sin embargo, junto con BAT 104 y Jamapa fueron las de menor tamaño. BAT 104, fue la variedad en que se detectó el mayor contenido de proteína total (27,3 %) contrastando con Bayo Río Grande (23,3%). En promedio de las cuatro variedades el contenido de ambos aminoácidos resultó mayor en los extractos salinos (43 % L-met y 92 % L-trp). En consecuencia las determinaciones subsecuentes se realizaron únicamente en los extractos salinos de los materiales. Las variedades Bayo Río Grande y Canario 107 tuvieron la mayor cantidad de L-met y L-trp, respectivamente

Autologous versus allogeneic versus umbilical cord sera for the treatment of severe dry eye disease : a double-blind randomized clinical trial

Funding Information: The research work presented here has been funded through a grant awarded by the Ministry of Health within the framework of the Aid for Independent Clinical Research of 2011 (EC11‐506), whose promoter is the Andalusian Public Foundation for Research on Malaga in Biomedicine and Health (FIMABIS). Publisher Copyright: © 2021 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons LtdPurpose: To measure the effects of Autologous serum (AS), Allogeneic Serum (HS) and Umbilical Cord serum (CS) eye drops in severe dry eye disease (DES), as well as to characterize and quantify several molecules in the three sera (albumin, fibronectin; Vitamin A and E; IgG, IgA and IgM; Transforming growth factor β; Epithelial growth factor). Methods: Randomized, double-blind, single-centre, three-arm (AS, HS and CS) clinical trial. Sixty-three subjects were included with severe DES, 21 in each arm of the study. Visual acuity, Schirmer test, Breakup time (BUT), lissamine green, fluorescein staining measurements and a questionnaire were performed prior to treatment, and after one-month and three-month follow-up. Results: There was a significant main effect of time on visual acuities, Schirmer and BUT tests and fluorescein and lissamine green staining measurements and questionnaire scores (p = 0.015, p = 0.002, p < 0.001, p < 0.001, p = 0.031 and p < 0.001, respectively), although there was no significant interaction between time and serum type, nor between serum type and the test performed. Regarding the concentration of molecules, in our study AS contained significantly higher concentrations of IgA, IgG and fibronectin whereas HS contained significantly higher concentration of IgM, vitamins A and E, TGF and albumin. Contrary to previous reports, CS did not show higher concentration of any of the molecules analysed. Conclusions and relevance: The three sera were effective in the treatment of severe DES. CS did not contain a higher proportion of molecules compared to AS/HS. More research is needed to assess the effect of AS in patients with DES and autoimmune diseases.Peer reviewe

Spatio-temporal tumor heterogeneity in metastatic CRC tumors: a mutational-based approach

[EN] It is well known that activating mutations in the KRAS and NRAS genes are associated with poor response to anti-EGFR therapies in patients with metastatic colorectal cancer (mCRC). Approximately half of the patients with wild-type (WT) KRAS colorectal carcinoma do not respond to these therapies. This could be because the treatment decision is determined by the mutational profile of the primary tumor, regardless of the presence of small tumor subclones harboring RAS mutations in lymph nodes or liver metastases. We analyzed the mutational profile of the KRAS, NRAS, BRAF and PI3KCA genes using low-density microarray technology in samples of 26 paired primary tumors, 16 lymph nodes and 34 liver metastases from 26 untreated mCRC patients (n=76 samples). The most frequent mutations found in primary tumors were KRAS (15%) and PI3KCA (15%), followed by NRAS (8%) and BRAF (4%). The distribution of the mutations in the 16 lymph node metastases analyzed was as follows: 4 (25%) in KRAS gene, 3 (19%) in NRAS gene and 1 mutation each in PI3KCA and BRAF genes (6%). As expected, the most prevalent mutation in liver metastasis was in the KRAS gene (35%), followed by PI3KCA (9%) and BRAF (6%). Of the 26 cases studied, 15 (58%) displayed an overall concordance in the mutation status detected in the lymph node metastases and liver metastases compared with primary tumor, suggesting no clonal evolution. In contrast, the mutation profiles differed in the primary tumor and lymph node/metastases samples of the remaining 11 patients (48%), suggesting a spatial and temporal clonal evolution. We confirm the presence of different mutational profiles among primary tumors, lymph node metastases and liver metastases. Our results suggest the need to perform mutational analysis in all available tumor samples of patients before deciding to commence anti-EGFR treatment

Puesta en marcha del curso on line “Envejecimiento y enfermedades neurodegenerativas” en la plataforma Miriadax

Este informe detalla los objetivos, actividades y métodos llevados a cabo durante la ejecución de un proyecto de innovación docente que tiene como objetivo implementar un curso online en abierto sobre las enfermedades neurodegenerativas y el envejecimiento

Elaboración de metodologías, materiales y recursos didácticos para la docencia de la filosofía en educación secundaria, estudios de grado y estudios de posgrado

Memoria ID2022-061. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2022-2023

Efecto de la cocción sobre algunas características nutricionales del frijol.

Effect of cooking on some nutritional characteristics of beans. Seven bean varieties of the seed types: Flor de Mayo, Negro, Bayo, and Canario, and the line BAT 104 were included in this study. The objective of this research was to analyze the technological and nutritive characteristics as well as the changes that result from cooking in the digestibility and the activity of the trypsin inhibitor of the materials. Highly significant differences among varieties in weight, volume, coat percentage, cooking time, trypsin inhibitor activity and digestibility were found. The protein content in the materials ranged from 22.6 to 27.3 %. Also, differences were detected among materials in the chemical components such as starch, crude fiber, ash, lysine and tryptophan content. A low activity of the trypsin inhibitor in cooked beans was more correlated with longer cooking time and smaller grains rather than with the inhibitor content in raw beans. Digestibility of the materials was increased 11% on the average as a consequence of cooking.Se estudiaron siete variedades de frijol de los tipos: Flor de Mayo, Negro, Bayo y Canario, además de la línea BAT 104. El objetivo del presente estudio fue analizar las características tecnológicas y nutricionales, así como los cambios que por efecto de la cocción resultan en la digestibilidad de la proteína y la actividad del inhibidor de tripsina en el grano de los materiales. Se encontraron diferencias significativas entre variedades en el peso, tamaño, porcentaje de testa, tiempo de cocción, actividad del inhibidor de tripsina y digestibilidad. El contenido de proteína en los materiales varió de 22.6 a 27.3 %; asimismo se detectaron diferencias entre los materiales en su concentración de componentes químicos, tales como almidón, fibra cruda, cenizas, lisina y triptofano. Una baja actividad del inhibidor de tripsina en el frijol cocido estuvo mas correlacionada con un mayor tiempo de cocción y menor tamaño de grano que con el contenido del mismo inhibidor en el frijol crudo. La digestibilidad de los materiales se incrementó en promedio 10 % como consecuencia de la cocción

DARKENING OF THE SEED COAT IN DRY BEAN GENOTYPES DURING EARLY DAYS AFTER HARVEST

INTRODUCTION As it is a center of origin of dry beans (Phaseolus vulgaris L.), in Mexico there are a wide variety of colors and shapes that are consumed. Post harvest, darkening of the seed coat is a problem in light colored beans, which decreases its marketability. In the lab, coat color is evaluated by reflectance spectrophotometry and it is used as a parameter for selection in genetic improvement. Although this trait exhibits a clear genotype-environment interaction, it is possible to select genotypes with greater color stability during storage, which is associated with increased in shelf life. In previous studies (Jacinto et al. 2006, 2007) polyphenol oxidase activity has been associated with the proneness of genotypes to darkening during storage. To select genotypes less prone to darkening of seed coat, accelerated aging is induced in the beans by increasing the temperature and relative humidity, this process implies time and work. The objective of this study was to assess the tendency of a group of eleven genotypes to darken during the first 44 days after harvesting. MATERIALS AND METHODS During PV 2014, eleven dry bean genotypes were sown at Santa Lucía de Prías, Texcoco, estado de México. The experimental plot was one 4 m- long row. Except for one black seed coat variety, the other 10 genotypes were light colored either with pattern or one single color. Upon reaching maturity, the plants of each plot were hand threshed. The first day after threshing color was measured using a CM-5 spectrophotometer (Konica Minolta, Inc., Osaka, Japan). Color reflectance was recorded in the CIE Lab color coordinate system, with D65 Illuminant and 10º observer. Samples were then left at room temperature in glass cases for 44 days. During 28 days color measurements every two to three days were taken; then it was measured at 44 days. Data was processed through an analysis of variance and a correlation test