114 research outputs found
Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex
10.1371/journal.pone.0081734PLoS ONE812-POLN
Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers
We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem’s influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cell migration, we applied label-free digital holographic microscopy (DHM) and single-cell tracking. Moreover, quantitative DHM phase images were used as novel stain-free assay to quantify the temporal course of global cellular morphology changes in confluent cell layers. Cytoskeleton alterations and tight junction protein redistributions were complementary analyzed by fluorescence microscopy. Calcium influx measurements were conducted to characterize the influence of the nanoformulations and capsaicin on ion channel activities. We found that both, capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, have a significant impact on directed cell migration and cellular motility. Increase of velocity and directionality of cell migration correlates with changes in the cell layer surface roughness, tight junction integrity and cytoskeleton alterations. Calcium influx into cells occurred only after nanoformulation treatment but not upon addition of free capsaicin. Our results pave the way for further studies on the biological significance of these findings and potential biomedical applications, e.g. as drug and gene carriers
Intercomparison of real-time tailpipe ammonia measurements from vehicles tested over the new world-harmonized light-duty vehicle test cycle (WLTC)
Mental health and school dropout across educational levels and genders: a 4.8-year follow-up study
Efficacy and safety of statin therapy in older people: a meta-analysis of individual participant data from 28 randomised controlled trials
Australian National Health and Medical Research CouncilMRCBritish Heart Foundation
Efficacy and safety of haloperidol versus atypical antipsychotic medications in the treatment of delirium
Length scales and statistical characteristics of outer bank roughness for large elongate meander bends: The influence of bank material properties, floodplain vegetation and flow inundation
Objective Definition of Rainfall Intensity-Duration Thresholds for Post-fire Flash Floods and Debris Flows in the Area Burned by the Waldo Canyon Fire, Colorado, USA
Comparing single‐phase, non‐Newtonian approaches with experimental results: Validating flume‐scale mud and debris flow in HEC‐RAS
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