Characterization of genetic structure of alfalfa (Medicago sp.) from trans-Himalaya using RAPD and ISSR markers

Twenty five (25) accessions of Lucerne (Medicago sp.) collected from Leh valley of trans-Himalayan region of Ladakh (Jammu and Kashmir) were analyzed using inter simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPD). The results of this study revealed that the level of genetic variation in the collected Medicago ecotypes were relatively high (P=96.54%, I=0.430, Ht=0.285). RAPD fingerprinting detected more polymorphic loci (97.96%) than ISSR fingerprinting (95.12%). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrogram were compared, whereas the pattern of clustering of the genotypes remained more or less the same in RAPD and combined data of RAPD + ISSR. The mean coefficient of differentiation (Gst) was 0.0584 indicating 30.23% of the genetic diversity within the populations. The overall value of mean estimated number of gene flow (Nm = 8.0682) revealed large gene exchanges among populations. Analysis of molecular variance (AMOVA) indicated that the distribution of genetic diversity was 49% among populations and 51% within populations. The plant is capable of reproducing by self-sowing, thus can influence population genetic structure. The pronounced genetic variation tells us that Medicago species is a proper plant for genetic research and that there is great potential of breeding this species for improved forage varieties.Key words: Genetic variation, ISSR, RAPD, Medicago species, population structure

Atmospheric aerosol and Doppler lidar studies

Experimental and theoretical studies were performed of atmospheric aerosol backscatter and atmospheric dynamics with Doppler lidar as a primary tool. Activities include field and laboratory measurement and analysis efforts. The primary focus of activities related to understanding aerosol backscatter is the GLObal Backscatter Experiment (GLOBE) program. GLOBE is a multi-element effort designed toward developing a global aerosol model to describe tropospheric clean background backscatter conditions that Laser Atmospheric Wind Sounder (LAWS) is likely to encounter. Two survey missions were designed and flown in the NASA DC-8 in November 1989 and May to June 1990 over the remote Pacific Ocean, a region where backscatter values are low and where LAWS wind measurements could make a major contribution. The instrument complement consisted of pulsed and continuous-wave (CW) CO2 gas and solid state lidars measuring aerosol backscatter, optical particle counters measuring aerosol concentration, size distribution, and chemical composition, a filter/impactor system collecting aerosol samples for subsequent analysis, and integrating nephelometers measuring visible scattering coefficients. The GLOBE instrument package and survey missions were carefully planned to achieve complementary measurements under clean background backscatter conditions

Euler/Navier-Stokes Solvers Applied to Ducted Fan Configurations

Due to noise considerations, ultra high bypass ducted fans have become a more viable design. These ducted fans typically consist of a rotor stage containing a wide chord fan and a stator stage. One of the concerns for this design is the classical flutter that keeps occurring in various unducted fan blade designs. These flutter are catastrophic and are to be avoided in the flight envelope of the engine. Some numerical investigations by Williams, Cho and Dalton, have suggested that a duct around a propeller makes it more unstable. This needs to be further investigated. In order to design an engine to safely perform a set of desired tasks, accurate information of the stresses on the blade during the entire cycle of blade motion is required. This requirement in turn demands that accurate knowledge of steady and unsteady blade loading be available. Aerodynamic solvers based on unsteady three-dimensional analysis will provide accurate and fast solutions and are best suited for aeroelastic analysis. The Euler solvers capture significant physics of the flowfield and are reasonably fast. An aerodynamic solver Ref. based on Euler equations had been developed under a separate grant from NASA Lewis in the past. Under the current grant, this solver has been modified to calculate the aeroelastic characteristics of unducted and ducted rotors. Even though, the aeroelastic solver based on three-dimensional Euler equations is computationally efficient, it is still very expensive to investigate the effects of multiple stages on the aeroelastic characteristics. In order to investigate the effects of multiple stages, a two-dimensional multi stage aeroelastic solver was also developed under this task, in collaboration with Dr. T. S. R. Reddy of the University of Toledo. Both of these solvers were applied to several test cases and validated against experimental data, where available

Application of Aeroelastic Solvers Based on Navier-Stokes Equations

A pre-release version of the Navier-Stokes solver (TURBO) was obtained from MSU. Along with Dr. Milind Bakhle of the University of Toledo, subroutines for aeroelastic analysis were developed and added to the TURBO code to develop versions 1 and 2 of the TURBO-AE code. For specified mode shape, frequency and inter-blade phase angle the code calculates the work done by the fluid on the rotor for a prescribed sinusoidal motion. Positive work on the rotor indicates instability of the rotor. The version 1 of the code calculates the work for in-phase blade motions only. In version 2 of the code, the capability for analyzing all possible inter-blade phase angles, was added. The version 2 of TURBO-AE code was validated and delivered to NASA and the industry partners of the AST project. The capabilities and the features of the code are summarized in Refs. [1] & [2]. To release the version 2 of TURBO-AE, a workshop was organized at NASA Lewis, by Dr. Srivastava and Dr. M. A. Bakhle, both of the University of Toledo, in October of 1996 for the industry partners of NASA Lewis. The workshop provided the potential users of TURBO-AE, all the relevant information required in preparing the input data, executing the code, interpreting the results and bench marking the code on their computer systems. After the code was delivered to the industry partners, user support was also provided. A new version of the Navier-Stokes solver (TURBO) was later released by MSU. This version had significant changes and upgrades over the previous version. This new version was merged with the TURBO-AE code. Also, new boundary conditions for 3-D unsteady non-reflecting boundaries, were developed by researchers from UTRC, Ref. [3]. Time was spent on understanding, familiarizing, executing and implementing the new boundary conditions into the TURBO-AE code. Work was started on the phase lagged (time-shifted) boundary condition version (version 4) of the code. This will allow the users to calculate non-zero interblade phase angles using, only one blade passage for analysis

CO2 lidar backscatter experiment

The Aerosol/Lidar Science Group of the Remote Sensing Branch engages in experimental and theoretical studies of atmospheric aerosol scattering and atmospheric dynamics, emphasizing Doppler lidar as a primary tool. Activities include field and laboratory measurement and analysis efforts by in-house personnel, coordinated with similar efforts by university and government institutional researchers. The primary focus of activities related to understanding aerosol scattering is the GLObal Backscatter Experiment (GLOBE) program. GLOBE was initiated by NASA in 1986 to support the engineering design, performance simulation, and science planning for the prospective NASA Laser Atmospheric Wind Sounder (LAWS). The most important GLOBE scientific result has been identified of a background aerosol mode with a surprisingly uniform backscatter mixing ratio (backscatter normalized by air density) throughout a deep tropospheric layer. The backscatter magnitude of the background mode evident from the MSFC CW lidar measurements is remarkably similar to that evident from ground-based backscatter profile climatologies obtained by JPL in Pasadena CA, NOAA/WPL in Boulder CO, and by the Royal Signals and Radar Establishment in the United Kingdom. Similar values for the background mode have been inferred from the conversion of in situ aerosol microphysical measurements to backscatter using Mie theory. Little seasonal or hemispheric variation is evident in the survey mission data, as opposed to large variation for clouds, aerosol plums, and the marine boundary layer. Additional features include: localized aerosol residues from dissipated clouds, occasional regions having mass concentrations of nanograms per cubic meter and very low backscatter, and aerosol plumes extending thousands of kilometers and several kilometers deep. Preliminary comparison with meteorological observations thus far indicate correlation between backscatter and water vapor under high humidity conditions. Limited intercomparisons with the Stratospheric Aerosol and Gas Experiment (SAGE) limb extinction sounder shows differences in the troposphere, however, it should be noted that in general SAGE measurements have not yet been validated in the troposphere

Massive Supersymmetric Quantum Gauge Theory

We continue the study of the supersymmetric vector multiplet in a purely quantum framework. We obtain some new results which make the connection with the standard literature. First we construct the one-dimensional physical Hilbert space taking into account the (quantum) gauge structure of the model. Then we impose the condition of positivity for the scalar product only on the physical Hilbert space. Finally we obtain a full supersymmetric coupling which is gauge invariant in the supersymmetric sense in the first order of perturbation theory. By integrating out the Grassmann variables we get an interacting Lagrangian for a massive Yang-Mills theory related to ordinary gauge theory; however the number of ghost fields is doubled so we do not obtain the same ghost couplings as in the standard model Lagrangian.Comment: 13 pages, no figure

Poultry Litter-Treated Length Effects on Quality of Runoff from Fescue Plots

Using experimental data and/or mathematical simulation models to identify practices that reduce pollution from manure-treated areas is sometimes perceived as limited by the unknown validity of extrapolating plot-scale data to larger areas and by uncertainties in modeling transport of various pollutants. The objectives of this study were to assess the effect of length of manure treatment on runoff concentrations of poultry litter constituents and to define the modes of transport (particulate versus soluble) for nitrogen (N), phosphorus (P), carbon (C), and solids. Poultry litter was applied to three 1.5- x 18.3-m fescue (Festuca arundinacea Schreb.) plots with runoff collection gutters installed at 3.0-m intervals along the lengths of the plots. Runoff was generated from simulated rainfall (50 mm/h for 1 h of runoff), and samples were analyzed for total Kjeldahl N (TKN), organic N (Org-N), ammonia N (NH3-N), nitrate N (NO3-N), total P (TP), total organic C (TOC), and total suspended solids (TSS). Soluble fractions of TKN, Org-N, NH3-N, TP, and TOC were also determined. Manure-treated length had no effect on runoff concentration of any parameter, indicating that a manure-treated length of only 3.0 m would have been sufficient to simulate runoff quality associated with longer manure length treatments. Proportions of TKN, Org-N, NH3-N, and TP transported in soluble form were high (≥ 74%), and over half of the TOC in the runoff was in soluble form. These results indicate that for conditions similar to those of this study, extrapolation with respect to runoff concentrations might be possible with little adaptation of the data and might simplify the design of management practices that key on edge-of-field runoff concentrations. The results with regard to modes of transport can help to better model losses of N, P, and COD and suggest that losses of these parameters will be most effectively controlled through practices that focus on reducing soluble losses rather than simply reducing erosion

Understanding Deleted File Decay on Removable Media using Differential Analysis

Digital content created by picture recording devices is often stored internally on the source device, on either embedded or removable media. Such storage media is typically limited in capacity and meant primarily for interim storage of the most recent image files, and these devices are frequently configured to delete older files as necessary to make room for new files. When investigations involve such devices and media, it is sometimes these older deleted files that would be of interest. It is an established fact that deleted file content may persist in part or in its entirety after deletion, and identifying the nature of file fragments on digital media has been an active research area for years. However, very little research has been conducted to understand how and why deleted file content persists (or decays) on different media and under different circumstances. The research reported here builds upon prior work establishing a methodology for the study of deleted file decay generally, and the application of that methodology to the decay of deleted files on traditional computing systems with spinning magnetic disks. In this current work, we study the decay of deleted image files on a digital camera with removable SD card storage, and we conduct preliminary experiments for direct SD card and USB storage. Our results indicate that deleted file decay is affected by the size of both the deleted and overwriting files, overwrite frequency, sector size, and cluster size. These results have implications for digital forensic investigators seeking to recover and interpret file fragments

Performance of Vegetative Filter Strips with Varying Pollutant Source and Filter Strip Lengths

Vegetative filter strips (VFS) can reduce runoff losses of pollutants such as nitrogen (N) and phosphorus (P) from land areas treated with fertilizers. While VFS effectiveness is considered to depend on lengths of pollutant source and VFS areas, there is little experimental evidence of this dependence, particularly when the pollutant source is manure-treated pasture. This study assessed the effects of pollutant source area (fescue pasture treated with poultry litter) length and VFS (fescue pasture) length on VFS removal of nitrate N (NO3-N), ammonia N (NH3-N), total Kjeldahl N (TKN), ortho-P (PO4-P), total P (TP), total organic carbon (TOC), total suspended solids (TSS), and fecal coliform (FC) from incoming runoff. This research examined poultry litter-treated lengths of 6.1, 12.2, and 18.3 m, with corresponding VFS lengths of up to 18.3 m, 12.2 m, and 6.1 m, respectively. Runoff was produced from simulated rainfall applied to both the litter-treated and VFS areas at 50 mm/h for 1 h of runoff. Pollutant concentrations in runoff were unaffected by litter-treated length but demonstrated a first-order exponential decline with increasing VFS length except for TSS and FC. Runoff mass transport of NH3-N,TKN, PO4-P, TP and TOC increased with increasing litter-treated length (due to increased runoff) and decreased (approximately first-order exponential decline) with increasing VFS length when affected by VFS length. Effectiveness of the VFS in terms of NH3-N, TKN, PO4-P, TP and TOC removal from runoff ranged from 12-75, 22-67, 22-82, 21-66, and 8-30% respectively. The data from this study can help in developing and testing models that simulate VFS performance and thus aid in the design of VFS installed downslope of pasture areas treated with animal manure