Modelling soil organic carbon changes under different maize cropping scenarios for cellulosic ethanol in Europe

The utilization of crop residues in the production of second generation biofuels has the potential to boost the bioenergy sector without affecting food commodity prices. However, policies leading to large-scale biomass removal should carefully balance the consequences, both environmental and in terms of emissions, on soil organic carbon (SOC) stocks depletion. Using a recently developed simulation platform, SOC changes were estimated at European level (EU + candidate and potential candidate countries) under two scenarios of low (R30) and high (R90) maize stover removal for cellulosic ethanol production (i.e. 30% and 90% of stover removal, respectively). Additionally, mitigation practices for SOC preservation, namely the introduction of a ryegrass cover crop (R90_C) and biodigestate return to soil (R90_B), were explored under the highest rate of stover removal. The results showed that 15.3 to 50.6 Mt yr-1 of stover (dry matter) would be potentially available for ethanol production under the lower and high removal rates considered. However, large-scale exploitation of maize residues will lead to a SOC depletion corresponding to 39.7 – 135.4 Mt CO2 eq. by 2020 (under R30 and R90, respectively) with greater losses in the long-term. In particular, every tonne of C residue converted to bioethanol was predicted to have an additional impact on SOC loss almost ranging from 0.2-0.5 CO2 eq ha-1 yr-1, considering a continuous biofuel scenario by 2050. The mitigation practices evaluated could more than halve SOC losses compared to R90, but not totally offsetting the negative soil C balance. There is a pressing need to design policies at EU level for optimum maize biofuel cultivations that will preserve the current SOC stock or even generate C credits.JRC.H.5-Land Resources Managemen

Phosphatidylinositol 3-kinase regulation of fluid phase endocytosis

AbstractEndocytosis of the fluid phase marker, horse radish peroxidase, into baby hamster kidney cells is inhibited by treatment of cells with the fungal metabolite wortmannin. The IC50 of approximately 5 nM is consistent with the well-described action of wortmannin upon phosphatidylinositol (PI) 3-kinase. Analysis of the kinetics of uptake indicates a > 50% decrease in the initial rate of marker internalisation, a concomitant decrease in the volume of the early endosome and an increased efficiency of recycling of that marker which is internalised. As PI 3-kinase binds to activated growth factor receptors our data suggest that receptor activation can be coupled to receptor internalisation (down regulation) by localising PI 3-kinase stimulation of endocytosis

Monitoring sediment transfer processes on the desert margin

LANDSAT Thematic Mapper and Multispectral Scanner data have been used to construct change detection images for three playas in south-central Tunisia. Change detection images have been used to analyze changes in surface reflectance and absorption between wet and dry season (intra-annual change) and between different years (inter-annual change). Change detection imagery has been used to examine geomorphological changes on the playas. Changes in geomorphological phenomena are interpreted from changes in soil and foliar moisture levels, differences in reflectances between different salt and sediments and the spatial expression of geomorphological features. Intra-annual change phenomena that can be detected from multidate imagery are changes in surface moisture, texture and chemical composition, vegetation cover and the extent of aeolian activity. Inter-annual change phenomena are divisible into those restricted to marginal playa facies (sedimentation from sheetwash and alluvial fans, erosion from surface runoff and cliff retreat) and these are found in central playa facies which are related to the internal redistribution of water, salt and sediment

Strategic trastuzumab mediated crosslinking driving concomitant HER2 and HER3 endocytosis and degradation in breast cancer

Efficacious anticancer therapies for targeting plasma membrane receptors with antibody based therapeutics are often contingent on sufficient endocytic delivery of receptor and conjugate to lysosomes. This results in downregulation of receptor activity and, in the case of antibody-drug conjugates (ADCs), intracellular release of a drug payload. The oncogenic receptor HER2 is a priority therapeutic target in breast cancer. Known as an “endocytosis resistant” receptor, HER2 thwarts the receptor downregulating efficiency of the frontline treatment trastuzumab and reduces the potential of trastuzumab-based therapies such as trastuzumab-emtansine. We previously demonstrated that strategically inducing trastuzumab and HER2 crosslinking in breast cancer cells promoted endocytosis and lysosomal delivery of the HER2-trastuzumab complex, stimulating downregulation of the receptor. Here we reveal that HER3, but not EGFR, is also concomitantly downregulated with HER2 after crosslinking. This is accompanied by strong activation of MEK/ERK pathway that we show does not directly contribute to HER2/trastuzumab endocytosis. We show that crosslinking induced trastuzumab endocytosis occurs via clathrin-dependent and independent pathways and is an actin-dependent process. Detailed ultrastructural studies of the plasma membrane highlight crosslinking-specific remodelling of microvilli and induction of extensive ruffling. Investigations in a cell model of acquired trastuzumab resistance demonstrate, for the first time, that they are refractory to crosslinking induced HER2 endocytosis and downregulation. This implicates further arrest of HER2 internalisation in developing trastuzumab resistance. Overall our findings highlight the potential of receptor crosslinking as a therapeutic strategy for cancer while exposing the ability of cancer cells to develop resistance via endocytic mechanisms

LUCAS 2018 - SOIL COMPONENT: Sampling Instructions for Surveyors

The European Commission launched a soil assessment component to the periodic LUCAS Land Use/Land Cover Area Frame Survey in 2009. Composite soil samples from 0-20-cm depth were taken, air-dried and sieved to 2 mm in order to analyse physical and chemical parameters of topsoil in 25 Member States (EU-27 except Bulgaria, Romania, Malta and Cyprus). The aim of the LUCAS Soil Component was to create a harmonised and comparable dataset of main properties of topsoil at the EU. The LUCAS Soil Component was extended to Bulgaria and Romania in 2012. Overall, ca. 22,000 soil samples were collected and analysed. All samples were analysed for percentage of coarse fragments, particle-size distribution, pH, organic carbon, carbonates, phosphorous, total nitrogen, extractable potassium, cation exchange capacity, multispectral properties and heavy metals. In 2015, the soil sampling was repeated in the same set of points of LUCAS 2009/2012 to monitor changes in topsoil physical and chemical parameters across the EU. The soil component was extended to points above elevations of 1000 m, which were not sampled in LUCAS 2009/2012. Furthermore, soil samples were taken in Albania, Bosnia-Herzegovina, Croatia, Macedonia, Montenegro, Serbia and Switzerland. The soil sampling was carried out following the instructions already used in LUCAS 2009/2012. Approximately 27,000 samples were collected and will be analysed during 2016 and 2017. In 2018, a new soil sampling campaign will be carried out within the LUCAS framework. Soil samples will be taken in repeated points of LUCAS 2009/2012 and LUCAS 2015. The novelty of the survey is that new physical, chemical and biological parameters will be analysed. Key parameters for evaluating soil quality, such as bulk density and soil biodiversity, will be analysed. These analyses require specific methods of soil sampling, preparation and storage of samples. Furthermore, field measurements such as the thickness of organic layer in peat soils, and visual assessment of signs of soil erosion will be carried out in 2018. This technical report compiles the instructions for collecting the various soil samples and for performing field measurements in the soil survey of 2018. These instructions will be used for all LUCAS surveyors, to create a comparable database of soil characteristics all over Europe.JRC.D.3-Land Resource

Effect of Good Agricultural and Environmental Conditions on erosion and soil organic carbon balance: A national case study

Since, the Common Agricultural Policies (CAP) reform in 2003, many efforts have been made at the European level to promote a more environmentally friendly agriculture. In order to oblige farmers to manage their land sustainably, the GAEC (Good Agricultural and Environmental Conditions) were introduced as part of the Cross Compliance mechanism. Among the standards indicated, the protection of soils against erosion and the maintenance of soil organic matter and soil structure were two pillars to protect and enhance the soil quality and functions. While Member States should specifically define the most appropriate management practices and verify their application, there is a substantial lack of knowledge about the effects of this policy on erosion prevention and soil organic carbon (SOC) change. In order to fill this gap, we coupled a high resolution erosion model based on Revised Universal Soil Loss Equation (RUSLE) with the CENTURY biogeochemical model, with the aim to incorporate the lateral carbon fluxes occurring with the sediment transportation. Three scenarios were simulated on the whole extent of arable land in Italy: (i) a baseline without the GAEC implementation; (ii) a current scenario considering a set of management related to GAEC and the corresponding area of application derived from land use and agricultural management statistics and (iii) a technical potential where GAEC standards are applied to the entire surface. The results show a 10.8% decrease, from 8.33 Mg ha −1 year −1 to 7.43 Mg ha −1 year −1 , in soil loss potential due to the adoption of the GAEC conservation practices. The technical potential scenario shows a 50.1% decrease in the soil loss potential (soil loss 4.1 Mg ha −1 year −1 ). The GAEC application resulted in overall SOC gains, with different rates depending on the hectares covered and the agroecosystem conditions. About 17% of the SOC change was attributable to avoided SOC transport by sediment erosion in the current scenario, while a potential gain up to 23.3 Mt of C by 2020 is predicted under the full GAEC application. These estimates provide a useful starting point to help the decision-makers in both ex-ante and ex-post policy evaluation while, scientifically, the way forward relies on linking biogeochemical and geomorphological processes occurring at landscape level and scaling those up to continental and global scales

Environmental Assessment of Soil for Monitoring: Volume IIb Survey of National Networks

The ENVASSO Project (Contract 022713) was funded 2006-8, under the European Commission 6th Framework Programme of Research, with the objective of defining and documenting a soil monitoring system appropriate for soil protection at continental level. The ENVASSO Consortium, comprising 37 partners drawn from 25 EU Member States, reviewed almost 300 soil indicators, identified existing soil inventories and monitoring programmes in the Member States, designed and programmed a database management system to capture, store and supply soil profile data, and drafted procedures and protocols appropriate for inclusion in a European soil monitoring network of sites that are geo-referenced and at which a qualified sampling process is or could be conducted. This volume (IIb), a Survey of National Networks, is the second of two reports that together constitute the most comprehensive study to date of the soil inventory and monitoring activities in the European Union. It contains comprehensive fact sheets for each national network, listing the purpose, sampling strategy adopted, analytical methods used and the number of monitoring sites.JRC.H.7-Climate Risk Managemen