2,842 research outputs found

    Probing Small-Molecule Microarrays with Tagged Proteins in Cell Lysates

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    The technique of small-molecule microarray (SMM) screening is based on the ability of small molecules to bind to various soluble proteins. This type of interaction is easily detected by the presence of a fluorescence signal produced by labeled antibodies that specifically recognize a unique sequence (tag) present on the target protein. The fluorescent signal intensity values are determined based on signal-to-noise ratios (SNRs). SMM screening is a high-throughput, unbiased method that can rapidly identify novel direct ligands for various protein targets. This binding-based assay format is generally applicable to most proteins, but it is especially useful for protein targets that do not possess an enzymatic activity. SMMs enable screening a protein in a purified form or in the context of a cellular lysate, likely providing a more physiologically relevant screening environment.National Cancer Institute (U.S.) (CA160860

    Objective pilling evaluation of wool fabrics

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    An objective pilling evaluation method based on the multi-scale two-dimensional dual-tree complex wavelet transform and linear discriminant function of Bayes\u27 Rule was developed. The surface fuzz and pills are identified from the high-frequency noise, fabric textures, fabric surface unevenness, and illuminative variation of a pilled fabric image by the two-dimensional dual-tree complex wavelet decomposition and reconstruction. The energies of the reconstructed subimages in six spatial orientations (±15º, ±45º, ±75º) are calculated as the elements of the pilling feature vector, whose dimension is reduced by principal component analysis. A linear discriminant function of Bayes\u27 Rule was used as a classifier to establish classification rules among the five pilling grades. A new pilled sample with the same physical construction can then be automatically assigned to one of the five pilling grades by the classification rules. A general evaluation of the proposed method was conducted using the SM50 woven, non-woven, and SM54 knitted standard pilling test image sets. The results suggest that the new method can successfully establish classification rules among the five pilling grade groups for each of the three standard pilling test image sets and should be applicable to practical objective pilling evaluation

    Shikimate pathway in apicomplexan parasites

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    Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses.

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    Spontaneous electrical activity generated by developing sensory cells and neurons is crucial for the maturation of neural circuits. The full maturation of mammalian auditory inner hair cells (IHCs) depends on patterns of spontaneous action potentials during a 'critical period' of development. The intrinsic spiking activity of IHCs can be modulated by inhibitory input from cholinergic efferent fibres descending from the brainstem, which transiently innervate immature IHCs. However, it remains unknown whether this transient efferent input to developing IHCs is required for their functional maturation. We used a mouse model that lacks the α9-nicotinic acetylcholine receptor subunit (α9nAChR) in IHCs and another lacking synaptotagmin-2 in the efferent terminals to remove or reduce efferent input to IHCs, respectively. We found that the efferent system is required for the developmental linearization of the Ca(2+)-sensitivity of vesicle fusion at IHC ribbon synapses, without affecting their general cell development. This provides the first direct evidence that the efferent system, by modulating IHC electrical activity, is required for the maturation of the IHC synaptic machinery. The central control of sensory cell development is unique among sensory systems

    Combined effect of coherent Z exchange and the hyperfine interaction in atomic PNC

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    The nuclear spin-dependent parity nonconserving (PNC) interaction arising from a combination of the hyperfine interaction and the coherent, spin-independent, PNC interaction from Z exchange is evaluated using many-body perturbation theory. For the 6s-7s transition in 133Cs, we obtain a result that is about 40% smaller than that found previously by Bouchiat and Piketty [Phys. Lett. B 269, 195 (1991)]. Applying this result to 133Cs, leads to an increase in the experimental value of nuclear anapole moment and exacerbates differences between constraints on PNC meson coupling constants obtained from the Cs anapole moment and those obtained from other nuclear parity violating experiments. Nuclear spin-dependent PNC dipole matrix elements, including contributions from the combined weak-hyperfine interaction, are also given for the 7s-8s transition in 211Fr and for transitions between ground-state hyperfine levels in K, Rb, Cs, Ba+, Au, Tl, Fr, and Ra+.Comment: Revtex4 preprint 19 pages 4 table

    Relative value of ruminally degradable and undegradable protein on the utilization of low-quality prairie hay by steers

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    An experiment was performed to investigate the impact of providing six levels of ruminally degradable protein (RDP; protein that is available to ruminal microbes) in combination with two levels of ruminally undegradable protein (RUP; protein that is not available to the ruminal microbes, but can be digested directly by cattle) on the intake and digestion of low-quality prairie hay. Twelve steers were provided unlimited access to low-quality prairie hay (5.3% crude protein and 71.7% neutral detergent fiber) throughout the trial. To simulate dietary RUP, casein was infused abomasally once daily at either 0 or 0.087% of body weight. To simulate dietary RDP, casein was infused ruminally once daily at 0, 0.029, 0.058, 0.087, 0.116, or 0.145% of body weight. As provision of RDP increased, forage intake and fiber digestion increased. Supplementing with RUP alone increased forage intake but not fiber digestion, although the intake response was not as large as providing the same amount of RDP. In conclusion, RUP is less efficient than RDP in stimulating forage intake and digestion
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