A co-culture microplate platform to quantify microbial interactions and growth dynamics

This thesis reports the development of BioMe, a co-culture microplate platform that enables high-throughput, real-time quantitative growth dynamics measurements of interacting microbial batch cultures. The primary BioMe components can be 3D-printed, allowing ease of fabrication and DIY accessibility in the microbiome community. A pairwise 3D-printed iteration of the BioMe device was used in diffusion and co-culture experiments. Genetically engineered Escherichia Coli lysine and isoleucine auxotroph strains were used to characterize the diffusion of amino acids across the porous membranes. Results demonstrated a nonlinear relationship between growth rate and pore size and also distinct diffusion behavior for lysine and isoleucine. Pairwise syntrophic co-culture experiments demonstrated synergistic but repressed interaction between these two paired auxotrophs. Investigation of the effect of varying initial amino acid conditions on growth dynamics demonstrated that small changes in initial media condition can consistently affect patterns of yield and growth rate of constituent microbial species.2020-08-30T00:00:00

The role of ICNA in Africa

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106854/1/j.1469-8749.2011.03972.x.pd

Whole body precooling attenuates the extracellular HSP72, IL-6 and IL-10 responses after an acute bout of running in the heat

The impact of whole-body precooling on the extracellular heat shock protein 72 (eHSP72) and cytokine responses to running in the heat is undefined. The aim of this study was to determine whether precooling would attenuate post-exercise eHSP72 and cytokine responses.Eight male recreational runnerscompleted two90-minuteboutsofrunningat65% _VO2maxin32±0.9°Cand47±6%relativehumidity(RH)preceded by either 60-minutes of precooling in 20.3 ± 0.3°C water (COOL) or 60 min rest in an air-conditioned laboratory (20.2 ± 1.7°C, 60 ± 3% RH; CON). eHSP72, TNF-α, IL-6, IL-10 IL-1ra were determined before and immediately after exercise. The elevation in post-exercise eHSP72 was attenuated after COOL (+0.04 ± 0.10 ng.mL−1) compared to CON (+ 0.29 ± 0.26 ng.mL−1;P < 0.001). No changes in TNF-α were observed at any stage. COOL reduced the absolute post-exercise change in IL-6 (P = 0.011) and IL-10 (P = 0.03) compared to CON. IL-1ra followed this trend (P = 0.063). A precooling-induced attenuation of eHSP72 and proinflammatory cytokines may aid recovery during multi-day sporting events, but could be counterproductive if a training response or adaptation to environmental stress is a desired outcome

Krause & Spellmann: Scenes from Lost America

2011 Kentucky Folk Art Center exhibition catalog of artists Jo Neace Krause and Charles Spellman.https://scholarworks.moreheadstate.edu/kfac_exhibition_catalogs/1007/thumbnail.jp

Comparison of the Efficacy of two Anticonvulsants, Phenytoin and Valproate to Improve PCP and d-amphetamine Induced Deficits in a Reversal Learning Task in the Rat

Recent studies in our laboratory have shown that PCP (phencyclidine) and d-amphetamine induce a cognitive deficit in rats, in a paradigm of potential relevance for the pathology of schizophrenia. Atypical, but not classical antipsychotics and the anticonvulsant, lamotrigine have been shown to prevent a selective reversal learning deficit induced by PCP. In contrast, only haloperidol reversed the d-amphetamine-induced deficit. The present study aimed to explore the ability of two anticonvulsants with differing mechanism of action, valproate and phenytoin to attenuate the cognitive deficits induced by PCP and d-amphetamine in the reversal learning paradigm. PCP at 1.5 mg/kg and d-amphetamine at 0.5 mg/kg both produced a selective and significant reduction in performance of the reversal phase with no effect on the initial phase of the task in female-hooded Lister rats. Valproate (25–200 mg/kg) and phenytoin (25–50 mg/kg) had no effect on performance when administered alone. Valproate (100–200 mg/kg), whose principle action is thought to be the enhancement of GABA transmission, was unable to prevent the cognitive deficit induced by either PCP or d-amphetamine. Conversely, phenytoin (50 mg/kg), a use-dependent sodium channel inhibitor, significantly prevented the deficit induced by PCP, but not d-amphetamine. These results add to our earlier work with lamotrigine, and suggest that sodium channel blockade may be a mechanism by which some anticonvulsant drugs can prevent the PCP-induced deficit. These data have implications for the use of anticonvulsant drugs in the treatment of cognitive or psychotic disorders

A heterokaryon instability gene in the Rockefeller-Lindegren strains of Neurospora crassa and its possible relation to the het i gene in Oak Ridge-St. Lawrence strains

The het I/i genes in the Oak Ridge-St. Lawrence (OR-SL) strains of Neurospora crassa are unique among the het genes in that they do not cause cell death in incompatible heterokaryons. Instead, within certain nuclear ratios, forced heterokaryons are unstable. They become homokaryotic for the het I component, and stop growing. Similar alleles have now been found in Rockefeller-Lindegren (RL) strains. The latter differ from those of the OR-SL strains in that the heterokaryons become homokaryotic over a wide range of initial nuclear ratios. Evidence is presented that suggests the two sets of alleles may be the same

Kinematic Discovery of a Stellar Stream Located in Pisces

We report the kinematic discovery of the Pisces Stellar Stream (PSS), at Galactic longitude l ≈ 135° and –39° < b < –36°. We originally identified this halo substructure from velocities of red giant branch stars in the Sloan Digital Sky Survey (SDSS) Data Release 8, and confirmed its presence in turnoff stars from SDSS photometric data. The PSS is a narrow, kinematically cold tidal stream, with σ_(v, 0) ≈ 8 km s^(–1). Its metallicity is [Fe/H] ≈ –2.2, with ~0.3 dex dispersion. The color-magnitude signature of the stream turnoff, combined with our measured metallicity, places the PSS at a distance of 35 ± 3 kpc. The PSS is the same as the previously announced "Triangulum stream" and part of the proposed "stream a." We rule out an association of the PSS with other previously known Milky Way substructures in the same region of the sky

Allocating group-level payments for ecosystem services: experiences from a REDD+ pilot in Tanzania

Payments for ecosystem services (PES) typically reward landowners for managing their land to provide ecosystem services that would not otherwise be provided. REDD+—Reduced Emissions from Deforestation and Forest Degradation—is a form of PES aimed at decreasing carbon emissions from forest conversion and extraction in lower-income countries. A key challenge for REDD+ occurs when it is implemented at a group, rather than an individual landowner, level. Whilst achieving a group-level reduction relies on individuals changing their interaction with the forest, incentives are not aligned explicitly at the individual level. Rather, payments are made to a defined group as a single entity in exchange for verified reduced forest loss, as per a PES scheme. In this paper, we explore how REDD+ has been implemented in one multiple-village pilot in Tanzania with the village defining the group. Our findings suggest that considerable attention has been paid towards monitoring, reporting, verification (MRV), and equity. No explicit mechanism ensures individual compliance with the village-level PES, and few villages allocate funds for explicit enforcement efforts to protect the forest from illegal activities undertaken by individual group members or by outsiders. However, the development of village-level institutions, “social fencing,” and a shared future through equal REDD+ payments, factor into decisions that influence the level of compliance at the village level that the program will eventually achieve