Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles

Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. EVs’ cargo may include different players of MDR, such as microRNAS and drug-efflux pumps, which may be transferred from donor MDR cells to recipient drug-sensitive counterparts. The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. Our results showed that MDR cells released more EVs than their drug-sensitive counterparts and also that the drug-sensitive cells captured more EVs than their MDR counterparts. This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment.This article is a result of the project NORTE-01-0145-FEDER-000029, supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). We thank Spanish MINECO (SAF2015-66312 to JMF) and for the REDIEX (Spanish Excellence Network in Exosomes) and the Severo Ochoa Excellence Accreditation (SEV-2016-0644). The authors thank the Portuguese Foundation for Science and Technology (FCT) for the PhD grant of DS (SFRH/BD/98054/2013). Cristina P.R. Xavier is supported by FCT and Fundo Social Europeu (FSE), through the post-doc grant SFRH/BPD/122871/2016. The authors also acknowledge the European COST Action-European Network on Microvesicles and Exosomes in Health and Disease (ME-HaD, BM1202) for short-term mission fellowship (ECOST-STSM-BM1202-150317-083396) and Grupo Español de investigacion en Vesiculas Extracelulares for GEIVEX mobility fellowship which allowed the work of DS in CICbioGUNE

Thermal behaviour of the different parts of almond shells as waste biomass

The main aim of this study is to investigate the thermal behaviour of the different parts of almond shells produced in an almond industry as a waste biomass. For this purpose, several experiments have been conducted under laboratory conditions. After removing the mature almonds, the waste raw materials subject of this study were treated with distilled water (10 min) and separated in several parts. Taking into account their physical characteristics, they were: (a) complete shells: exocarp, mesocarp and endocarp without grinding (Sample C); (b) ground samples of complete shells, sieved under 0.2 mm (Sample M); (c) hard layers of the endocarp (Sample E); (d) internal layers of the endocarp (Sample I); and (e) mature drupes (Sample P) or skin, being constituted by the flexible part of green colour (fresh form) or yellow (after drying). The thermal behaviour of all these sample materials has been investigated using a laboratory furnace, with determination of ash contents and mass loss by progressive heating (120 min of holding time). Elemental and DTA-TG/DTG analyses of selected sample materials have been carried out. Although a complete study can be very complex, a first approach has been performed in this investigation. Results on thermal decomposition of this biomass waste have been presented to emphasize the main differences between sample materials of almond shells. These results have demonstrated the influence of several parameters, such as the particle size, and previous treatments in the thermal behaviour of the different parts of the almond shells, as showed in this investigation. Structural analysis of almond shells allowed to determine lignin, cellulose and hemicellulose. From the lignin content, it has been predicted the higher heating value (18.24 MJkg(-1)) of this waste as by-product of industrial interest. Other linear correlations to calculate this parameter have been applied with similar results in all these samples

CALIFA, the Calar Alto Legacy Integral Field Area survey IV. Third public data release

This paper describes the Third Public Data Release (DR3) of the Calar Alto Legacy Integral Field Area (CALIFA) survey. Science-grade quality data for 667 galaxies are made public, including the 200 galaxies of the Second Public Data Release (DR2). Data were obtained with the integral-field spectrograph PMAS/PPak mounted on the 3.5m telescope at the Calar Alto Observatory. Three different spectral setups are available: i) a low-resolution V500 setup covering the wavelength range 3745–7500 Å (4240-7140 Å unvignetted) with a spectral resolution of 6.0 Å (FWHM) for 646 galaxies, ii) a medium-resolution V1200 setup covering the wavelength range 3650–4840 Å (3650-4620 Å unvignetted) with a spectral resolution of 2.3 Å (FWHM) for 484 galaxies, and iii) the combination of the cubes from both setups (called COMBO) with a spectral resolution of 6.0 Å and a wavelength range between 3700-7500 Å (3700-7140 Å unvignetted) for 446 galaxies. The Main Sample, selected and observed according to the CALIFA survey strategy covers a redshift range between 0.005 and 0.03, spans the color-magnitude diagram and probes a wide range of stellar masses, ionization conditions, and morphological types. The Extension Sample covers several types of galaxies that are rare in the overall galaxy population and are therefore not numerous or absent in the CALIFA Main Sample. All the cubes in the data release were processed using the latest pipeline, which includes improved versions of the calibration frames and an even further improved image reconstruction quality. In total, the third data release contains 1576 datacubes, including ∼1.5 million independent spectra. It is available at http://califa.caha.es/DR3.CALIFA is the first legacy survey being performed at Calar Alto. The CALIFA collaboration would like to thank the IAA-CSIC and MPIAMPG as major partners of the observatory, and CAHA itself, for the unique access to telescope time and support in manpower and infrastructures. The CALIFA collaboration thanks also the CAHA staff for the dedication to this project. We thank the anonymous referee for his/her help in improving this article. SFS thanks the director of CEFCA, M. Moles, for his sincere support to this project. SFS thanks the CONACYT-125180 and DGAPA-IA100815 projects for providing him support in this study. RGB, RGD, and EP are supported by grants AYA2014-57490-P and JA-FQM-2828. SZ is supported by the EU Marie Curie Integration Grant “SteMaGE” Nr. PCIG12-GA-2012-326466 (Call Identifier: FP7-PEOPLE-2012 CIG). J. F-B. from grant AYA2013-48226-C3-1-P from the Spanish Ministry of Economy and Competitiveness (MINECO), as well as from the FP7 Marie Curie Actions of the European Commission, via the Initial Training Network DAGAL under REA grant agreement number 289313 B.G-L- acknowledges financial support by the Spanish MINECO under grants AYA2013- 41656-P and AYA2015-68217-P Support for L.G. is provided by the Ministry of Economy, Development, and Tourism’s Millennium Science Initiative through grant IC12009, awarded to The Millennium Institute of Astrophysics, MAS. L.G. also acknowledges support by CONICYT through FONDECYT grant 3140566. and AYA2013-42227-P from the Spanish Ministerio de Ciencia e Innovación and TIC 114 and PO08-TIC-3531 from Junta de Andalucía. AG acknowledges support from the FP7/2007-2013 under grant agreement n. 267251 (AstroFIt). RAM was funded by the Spanish programme of International Campus of Excellence Moncloa (CEI). JMA acknowledges support from the European Research Council Starting Grant (SEDmorph; P.I. V. Wild). IM and AdO acknowledge the support by the projects AYA2010-15196 from the Spanish Ministerio de Ciencia e Innovación and TIC 114 and PO08-TIC-3531 from Junta de Andalucía. AMI acknowledges support from Agence Nationale de la Recherche through the STILISM project (ANR-12-BS05-0016-02). MM acknowledges financial support from AYA2010-21887-C04-02 from the Ministerio de Economía y Competitividad. PSB acknowledges support from the Ramón y Cajal program, grant ATA2010-21322-C03-02 from the Spanish Ministry of Economy and Competitiveness (MINECO). CJW acknowledges support through the Marie Curie Career Integration Grant 303912. VW acknowledges support from the European Research Council Starting Grant (SEDMorph P.I. V. Wild) and European Career Re-integration Grant (Phiz-Ev P.I. V. Wild). YA acknowledges financial support from the Ramón y Cajal programme (RyC-2011-09461) and project AYA2013-47742-C4-3-P, both managed by the Ministerio de Economía y Competitividad, as well as the ‘Study of Emission-Line Galaxies with Integral-Field Spectroscopy’ (SELGIFS) programme, funded by the EU (FP7-PEOPLE-2013- IRSES-612701) within the Marie-Sklodowska-Curie Actions scheme. ROM acknowledges support from CAPES (Brazil) through a PDJ fellowship from project 88881.030413/2013-01, program CSF-PVE.This is the author accepted manuscript. The final version is available from EDP Sciences via http://dx.doi.org/10.1051/0004-6361/20162866