Regulation of the Fruit-Specific PEP Carboxylase SlPPC2 Promoter at Early Stages of Tomato Fruit Development

The SlPPC2 phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) gene from tomato (Solanum lycopersicum) is differentially and specifically expressed in expanding tissues of developing tomato fruit. We recently showed that a 1966 bp DNA fragment located upstream of the ATG codon of the SlPPC2 gene (GenBank AJ313434) confers appropriate fruit-specificity in transgenic tomato. In this study, we further investigated the regulation of the SlPPC2 promoter gene by analysing the SlPPC2 cis-regulating region fused to either the firefly luciferase (LUC) or the β-glucuronidase (GUS) reporter gene, using stable genetic transformation and biolistic transient expression assays in the fruit. Biolistic analyses of 5′ SlPPC2 promoter deletions fused to LUC in fruits at the 8th day after anthesis revealed that positive regulatory regions are mostly located in the distal region of the promoter. In addition, a 5′ UTR leader intron present in the 1966 bp fragment contributes to the proper temporal regulation of LUC activity during fruit development. Interestingly, the SlPPC2 promoter responds to hormones (ethylene) and metabolites (sugars) regulating fruit growth and metabolism. When tested by transient expression assays, the chimeric promoter:LUC fusion constructs allowed gene expression in both fruit and leaf, suggesting that integration into the chromatin is required for fruit-specificity. These results clearly demonstrate that SlPPC2 gene is under tight transcriptional regulation in the developing fruit and that its promoter can be employed to drive transgene expression specifically during the cell expansion stage of tomato fruit. Taken together, the SlPPC2 promoter offers great potential as a candidate for driving transgene expression specifically in developing tomato fruit from various tomato cultivars

An elevation of 0.1 light-seconds for the optical jet base in an accreting Galactic black hole system

Relativistic plasma jets are observed in many accreting black holes. According to theory, coiled magnetic fields close to the black hole accelerate and collimate the plasma, leading to a jet being launched. Isolating emission from this acceleration and collimation zone is key to measuring its size and understanding jet formation physics. But this is challenging because emission from the jet base cannot be easily disentangled from other accreting components. Here, we show that rapid optical flux variations from a Galactic black-hole binary are delayed with respect to X-rays radiated from close to the black hole by ~0.1 seconds, and that this delayed signal appears together with a brightening radio jet. The origin of these sub-second optical variations has hitherto been controversial. Not only does our work strongly support a jet origin for the optical variations, it also sets a characteristic elevation of <~10$^3$ Schwarzschild radii for the main inner optical emission zone above the black hole, constraining both internal shock and magnetohydrodynamic models. Similarities with blazars suggest that jet structure and launching physics could potentially be unified under mass-invariant models. Two of the best-studied jetted black hole binaries show very similar optical lags, so this size scale may be a defining feature of such systems