43 research outputs found
Mutator dynamics in sexual and asexual experimental populations of yeast
<p>Abstract</p> <p>Background</p> <p>In asexual populations, mutators may be expected to hitchhike with associated beneficial mutations. In sexual populations, recombination is predicted to erode such associations, inhibiting mutator hitchhiking. To investigate the effect of recombination on mutators experimentally, we compared the frequency dynamics of a mutator allele (<it>msh2</it>Δ) in sexual and asexual populations of <it>Saccharomyces cerevisiae</it>.</p> <p>Results</p> <p>Mutator strains increased in frequency at the expense of wild-type strains in all asexual diploid populations, with some approaching fixation in 150 generations of propagation. Over the same period of time, mutators declined toward loss in all corresponding sexual diploid populations as well as in haploid populations propagated asexually.</p> <p>Conclusions</p> <p>We report the first experimental investigation of mutator dynamics in sexual populations. We show that a strong mutator quickly declines in sexual populations while hitchhiking to high frequency in asexual diploid populations, as predicted by theory. We also show that the <it>msh2Δ </it>mutator has a high and immediate realized cost that is alone sufficient to explain its decline in sexual populations. We postulate that this cost is indirect; namely, that it is due to a very high rate of recessive lethal or strongly deleterious mutation. However, we cannot rule out the possibility that <it>msh2</it>Δ also has unknown directly deleterious effects on fitness, and that these effects may differ between haploid asexual and sexual populations. Despite these reservations, our results prompt us to speculate that the short-term cost of highly deleterious recessive mutations can be as important as recombination in preventing mutator hitchhiking in sexual populations.</p
Evolutionary connectionism: algorithmic principles underlying the evolution of biological organisation in evo-devo, evo-eco and evolutionary transitions
The mechanisms of variation, selection and inheritance, on which evolution by natural selection depends, are not fixed over evolutionary time. Current evolutionary biology is increasingly focussed on understanding how the evolution of developmental organisations modifies the distribution of phenotypic variation, the evolution of ecological relationships modifies the selective environment, and the evolution of reproductive relationships modifies the heritability of the evolutionary unit. The major transitions in evolution, in particular, involve radical changes in developmental, ecological and reproductive organisations that instantiate variation, selection and inheritance at a higher level of biological organisation. However, current evolutionary theory is poorly equipped to describe how these organisations change over evolutionary time and especially how that results in adaptive complexes at successive scales of organisation (the key problem is that evolution is self-referential, i.e. the products of evolution change the parameters of the evolutionary process). Here we first reinterpret the central open questions in these domains from a perspective that emphasises the common underlying themes. We then synthesise the findings from a developing body of work that is building a new theoretical approach to these questions by converting well-understood theory and results from models of cognitive learning. Specifically, connectionist models of memory and learning demonstrate how simple incremental mechanisms, adjusting the relationships between individually-simple components, can produce organisations that exhibit complex system-level behaviours and improve the adaptive capabilities of the system. We use the term “evolutionary connectionism” to recognise that, by functionally equivalent processes, natural selection acting on the relationships within and between evolutionary entities can result in organisations that produce complex system-level behaviours in evolutionary systems and modify the adaptive capabilities of natural selection over time. We review the evidence supporting the functional equivalences between the domains of learning and of evolution, and discuss the potential for this to resolve conceptual problems in our understanding of the evolution of developmental, ecological and reproductive organisations and, in particular, the major evolutionary transitions
Variable Mutation Rates as an Adaptive Strategy in Replicator Populations
For evolving populations of replicators, there is much evidence that the effect of mutations on fitness depends on the degree of adaptation to the selective pressures at play. In optimized populations, most mutations have deleterious effects, such that low mutation rates are favoured. In contrast to this, in populations thriving in changing environments a larger fraction of mutations have beneficial effects, providing the diversity necessary to adapt to new conditions. What is more, non-adapted populations occasionally benefit from an increase in the mutation rate. Therefore, there is no optimal universal value of the mutation rate and species attempt to adjust it to their momentary adaptive needs. In this work we have used stationary populations of RNA molecules evolving in silico to investigate the relationship between the degree of adaptation of an optimized population and the value of the mutation rate promoting maximal adaptation in a short time to a new selective pressure. Our results show that this value can significantly differ from the optimal value at mutation-selection equilibrium, being strongly influenced by the structure of the population when the adaptive process begins. In the short-term, highly optimized populations containing little variability respond better to environmental changes upon an increase of the mutation rate, whereas populations with a lower degree of optimization but higher variability benefit from reducing the mutation rate to adapt rapidly. These findings show a good agreement with the behaviour exhibited by actual organisms that replicate their genomes under broadly different mutation rates
Incompatibilities Involving Yeast Mismatch Repair Genes: A Role for Genetic Modifiers and Implications for Disease Penetrance and Variation in Genomic Mutation Rates
Genetic background effects underlie the penetrance of most genetically determined phenotypes, including human diseases. To explore how such effects can modify a mutant phenotype in a genetically tractable system, we examined an incompatibility involving the MLH1 and PMS1 mismatch repair genes using a large population sample of geographically and ecologically diverse Saccharomyces cerevisiae strains. The mismatch repair incompatibility segregates into naturally occurring yeast strains, with no strain bearing the deleterious combination. In assays measuring the mutator phenotype conferred by different combinations of MLH1 and PMS1 from these strains, we observed a mutator phenotype only in combinations predicted to be incompatible. Surprisingly, intragenic modifiers could be mapped that specifically altered the strength of the incompatibility over a 20-fold range. Together, these observations provide a powerful model in which to understand the basis of disease penetrance and how such genetic variation, created through mating, could result in new mutations that could be the raw material of adaptive evolution in yeast populations
A Tradeoff Drives the Evolution of Reduced Metal Resistance in Natural Populations of Yeast
Various types of genetic modification and selective forces have been implicated
in the process of adaptation to novel or adverse environments. However, the
underlying molecular mechanisms are not well understood in most natural
populations. Here we report that a set of yeast strains collected from Evolution
Canyon (EC), Israel, exhibit an extremely high tolerance to the heavy metal
cadmium. We found that cadmium resistance is primarily caused by an enhanced
function of a metal efflux pump, PCA1. Molecular analyses
demonstrate that this enhancement can be largely attributed to mutations in the
promoter sequence, while mutations in the coding region have a minor effect.
Reconstruction experiments show that three single nucleotide substitutions in
the PCA1 promoter quantitatively increase its activity and thus
enhance the cells' cadmium resistance. Comparison among different yeast
species shows that the critical nucleotides found in EC strains are conserved
and functionally important for cadmium resistance in other species, suggesting
that they represent an ancestral type. However, these nucleotides had diverged
in most Saccharomyces cerevisiae populations, which gave cells
growth advantages under conditions where cadmium is low or absent. Our results
provide a rare example of a selective sweep in yeast populations driven by a
tradeoff in metal resistance
Variations in Stress Sensitivity and Genomic Expression in Diverse S. cerevisiae Isolates
Interactions between an organism and its environment can significantly influence
phenotypic evolution. A first step toward understanding this process is to
characterize phenotypic diversity within and between populations. We explored
the phenotypic variation in stress sensitivity and genomic expression in a large
panel of Saccharomyces strains collected from diverse
environments. We measured the sensitivity of 52 strains to 14 environmental
conditions, compared genomic expression in 18 strains, and identified gene
copy-number variations in six of these isolates. Our results demonstrate a large
degree of phenotypic variation in stress sensitivity and gene expression.
Analysis of these datasets reveals relationships between strains from similar
niches, suggests common and unique features of yeast habitats, and implicates
genes whose variable expression is linked to stress resistance. Using a simple
metric to suggest cases of selection, we found that strains collected from oak
exudates are phenotypically more similar than expected based on their genetic
diversity, while sake and vineyard isolates display more diverse phenotypes than
expected under a neutral model. We also show that the laboratory strain S288c is
phenotypically distinct from all of the other strains studied here, in terms of
stress sensitivity, gene expression, Ty copy number, mitochondrial content, and
gene-dosage control. These results highlight the value of understanding the
genetic basis of phenotypic variation and raise caution about using laboratory
strains for comparative genomics
Thirty years of Biology & Philosophy: philosophy of which biology?
Which domains of biology do philosophers of biology primarily study? The fact that
philosophy of biology has been dominated by an interest for evolutionary biology is
widely admitted, but it has not been strictly demonstrated. Here I analyse the topics of
all the papers published in Biology & Philosophy, just as the journal celebrates its
thirtieth anniversary. I then compare the distribution of biological topics in Biology &
Philosophy with that of the scientific journal Proceedings of the National Academy of
Science of the USA, focusing on the recent period 2003-2015. This comparison
reveals a significant mismatch between the distributions of these topics. I examine
plausible explanations for that mismatch. Finally, I argue that many biological topics
underrepresented in philosophy of biology raise important philosophical issues and
should therefore play a more central role in future philosophy of biology
Micro cam system driven by electrostatic comb-drive actuators based on SOI-MEMS technology
This paper reports a design and fabrication process of a micro cam system (MCS) with a flat-faced translating follower. The cam rim with cover diameter of 2.4 mm is driven by four electrostatic comb-drive actuators (ECA). Reciprocating motion of the ECAs is converted to unidirectional rotating movement of the outer cam rim through ratchet and anti-reverse mechanisms. The MCS was fabricated by using SOI (silicon on insulator) wafer with 30 μm thick device layer, and only one mask. Driven by periodic voltage with different frequencies, the performance of the MCS was successfully tested. In our experiment, when the system was run by the minimal driving voltage of 80 V, the obtained stroke of the follower was 160 μm. In addition, the system can run smoothly while applying the force of 31.3 μN on the follower. The MCS can be applied in micro valve structures, in micro assembling systems, etc