Developmental Reaction Norms for Water Stressed Seedlings of Succulent Cacti

Succulent cacti are remarkable plants with capabilities to withstand long periods of drought. However, their adult success is contingent on the early seedling stages, when plants are highly susceptible to the environment. To better understand their early coping strategies in a challenging environment, two developmental aspects (anatomy and morphology) in Polaskia chichipe and Echinocactus platyacanthus were studied in the context of developmental reaction norms under drought conditions. The morphology was evaluated using landmark based morphometrics and Principal Component Analysis, which gave three main trends of the variation in each species. The anatomy was quantified as number and area of xylem vessels. The quantitative relationship between morphology and anatomy in early stages of development, as a response to drought was revealed in these two species. Qualitatively, collapsible cells and collapsible parenchyma tissue were observed in seedlings of both species, more often in those subjected to water stress. These tissues were located inside the epidermis, resembling a web of collapsible-cell groups surrounding turgid cells, vascular bundles, and spanned across the pith. Occasionally the groups formed a continuum stretching from the epidermis towards the vasculature. Integrating the morphology and the anatomy in a developmental context as a response to environmental conditions provides a better understanding of the organism's dynamics, adaptation, and plasticity

Psychedelic resting-state neuroimaging: a review and perspective on balancing replication and novel analyses

Clinical research into serotonergic psychedelics is expanding rapidly, showing promising efficacy across myriad disorders. Resting-state functional magnetic resonance imaging (rs-fMRI) is a commonly used strategy to identify psychedelic-induced changes in neural pathways in clinical and healthy populations. Here we, a large group of psychedelic imaging researchers, review the 42 research articles published to date, based on the 17 unique studies evaluating psychedelic effects on rs-fMRI, focusing on methodological variation. Prominently, we observe that nearly all studies vary in data processing and analysis methodology, two datasets are the foundation of over half of the published literature, and there is lexical ambiguity in common outcome metric terminology. We offer guidelines for future studies that encourage coherence in the field. Psychedelic rs-fMRI will benefit from the development of novel methods that expand our understanding of the brain mechanisms mediating its intriguing effects; yet, this field is at a crossroads where we must also consider the critical importance of consistency and replicability to effectively converge on stable representations of the neural effects of psychedelics

Gene promoter hypermethylation in ductal lavage fluid from healthy BRCA gene mutation carriers and mutation-negative controls

INTRODUCTION: Female germline BRCA gene mutation carriers are at increased risk for developing breast cancer. The purpose of our study was to establish whether healthy BRCA mutation carriers demonstrate an increased frequency of aberrant gene promoter hypermethylation in ductal lavage (DL) fluid, compared with predictive genetic test negative controls, that might serve as a surrogate marker of BRCA1/2 mutation status and/or breast cancer risk. METHODS: The pattern of CpG island hypermethylation within the promoter region of a panel of four genes (RAR-β, HIN-1, Twist and Cyclin D2) was assessed by methylation-specific polymerase chain reaction using free DNA extracted from DL fluid. RESULTS: Fifty-one DL samples from 24 healthy women of known BRCA mutation status (7 BRCA1 mutation carriers, 12 BRCA2 mutation carriers and 5 controls) were available for methylation analysis. Eight of 19 (42.1%) BRCA mutation carriers were found to have at least one hypermethylated gene in the four-gene panel. Two BRCA mutation carriers, in whom aberrant methylation was found, also had duct epithelial cell atypia identified. No hypermethylation was found in DL samples from 5 negative controls(p = 0.13). CONCLUSION: We found substantial levels of aberrant methylation, with the use of a four-gene panel, in the fluid from the breasts of healthy BRCA mutation carriers compared with controls. Methylation analysis of free DNA in DL fluid may offer a useful surrogate marker for BRCA1/2 mutation status and/or breast cancer risk. Further studies are required for the evaluation of the specificity and predictive value of aberrant methylation in DL fluid for future breast cancer development in BRCA1/2 mutation carriers

A model of collaborative innovation between local government and tourism operators

[EN] This research proposes a framework for collaborative innovation in a public private partnership by applying techniques that combine quantitative data collection and qualitative depth. It proposes a collaborative model that looks to provide competitive advantage by improving tourist services from two perspectives: from the core of public administration, and from the private tourist sector perspective.Pons Morera, C.; Canós Darós, L.; Gil Pechuán, I. (2017). A model of collaborative innovation between local government and tourism operators. SERVICE BUSINESS. AN INTERNATIONAL JOURNAL. 1-26. doi:10.1007/s11628-017-0341-xS126Anderberg MR (1973) Cluster analysis for applications. Academic Press, New YorkAugustyn K (2000) Performance of tourism partnerships: a focus on York. Tour Manag 2:341–351Aziri B, Nedelea A (2013) Business strategies in tourism. Ecoforum 2(1):9Baglieri D, Consoli R (2009) Collaborative innovation in tourism: managing virtual communities. TQM J 21(4):353–364Barbero MI (2009) Métodos de elaboración de escalas. UNED, MadridBeaumont N, Dredge D (2010) Local tourism governance: a comparison of three network approaches. J Sustain Tourism 18(1):7–28Beritelli P, Bieger T, Laesser C (2007) Destination governance: Using corporate governance theories as a foundation for effective destination management. J Travel Res 46:96–107Bigné JE, Aldás J, Küster I, Vila N (2002) Estableciendo los determinantes de la fidelidad del cliente: Un estudio basado en técnicas cualitativas. Investigación y Mark 77:58–62Bruntland GH (1987) Our common future. http://www.un-documents.net/our-common-future.pdf . Consulted in 2016Buch EB, Cabaleiro R (2011) Hacia la determinación de la condición financiera de la administración publica local. Aplicación a los municipios de la comunidad autónoma de Galicia. Academia, Revista latinoamericana de administración, No. 47, pp. 43–60Burke JG, O’Campo P, Peak GL, Gielen AC, McDonnell KA, Trochim WM (2005) An introduction to concept mapping as a participatory public health research method. Qual Health Res 15(10):1392–1410Bustelo C, García-Morales E (2008) Administración electrónica y gestión documental. Consideraciones a la luz de la ley para el acceso electrónico de los ciudadanos a los servicios públicos. El profesional de la información. Enero-febrero 17(1):106–111Caffyn A, Jobbins G (2003) Governance capacity and stakeholder interactions in the development and management of coastal tourism: examples from Morocco and Tunisia. J Sustain Tourism 11(2):224–245Carlisle S, Kunc M, Jones E, Tiffin S (2013) Supporting innovation for tourism development through multi-stakeholder approaches: experiences from Africa. Tour Manag 35:59–69Clavero J, Codina M, Pérez A, Serrat-Brustenga M (2009) Estudio de caso de servicio de préstamo de libros electrónicos. El profesional de la información. Marzo-abril 18(2):237–241Czernek K (2013) Determinants of cooperation in a tourist region. Ann Tourism Res 40:83–104Dinica V (2009) Governance for sustainable tourism: a comparison of international and dutch visions. Journal of Sustainable Tourism. 17(5):583–603Dredge D, Whitford M (2011) Event tourism governance and the public sphere. J Sustain Tourism 19(4-5):479–499European Directive 2006/7/EC. Bathing Water. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2006:064:0037:0051:EN:PDF . Consulted in 2016Everitt B (1980) Cluster analysis. New York, NY, Halsted Press, a Division of John Wiley and SonsFernández O (1991) El análisis de Cluster: Aplicación, interpretación y validación. Papers 37:65–76Foundation for Environmental Quality of the European Union. http://www.blueflag.org/menu/awarded-sites/2014/northern-hemisphere/spain-2/List/Beaches . Consulted in 2016Freeman RE, McVea J (1984) A stakeholder approach to strategic management. Pitman, BostonGil-Lafuente AM, Kyun-Oh Y (2012) Incidencia de las inversiones de la administración pública sobre el desarrollo turístico de una ciudad. Revista Venezolana de Gestión Pública. Enero-Diciembre 3(3):81–109Gil-Pechuán I, Conesa-García M, Navarro-García A (2014) Concept mapping to improve higher education. Innovation and Teaching Technologies. ISBN: 978-3-319-04824-6. pp. 61–73Granovetter M (1985) Economic action and social structure: the problem of embeddedness. Am J Sociol 91(3):481Greenwood J (1993) Business interest groups in tourism governance. Tourism Manag 14:335–348Gulati R (1995) Social structure and alliance formation patterns: a longitudinal analysis. Adm Sci Q 40:619–653Henao-Betancur PA, Echeverri-Farley OM, Zartha-Sossa JW (2013) Metodología web para la formulación e implementación de estrategias de innovación en empresas. Rev Gestión de las Personas y Tecnol 16:6Hodge GA, Greve C (2017) On public-private partnership performance: a contemporary review. Public Works Manag Policy 22(1):55–78Hultman J, Hall CM (2012) Tourism place-Making governance of locality in Sweden. Ann Tourism Res 39(2):547–570Khattri N, Miles MB (1994) Cognitive mapping: a review and working guide. Center Policy Res, SparkhillKlodiana G, Dorina K, Engjellushe Z (2012) The role of local government in sustainable development. AUDOE. 8(2):139–155León JA, Zapico F (2003) Usos prácticos sobre la política electrónica en los servicios públicos: el caso de Extremadura. El profesional de la información. Mayo-junio 12(3):232–236Marinao E, Chasco C (2012) Trust in tourist destinations. the role of local inhabitants and institutions. Academia, Revista latinoamericana de administración 51:27–47Marzo-Roselló R, Peris-Pérez P, Ferris-Oñate, Sánchez-Lacuesta J, Matínez-Gómez L, Olaso-Melis J, Garcés-Pérez L, Primo-Capella VJ (2013) La experiencia turística en la ciudad de Valencia. Rev de Biomec 59:63–66Menon S, Manoj E (2014) Public private partnerships in tourism- a case study of Kerala Travel Mart. Afr J Hosp Tour Leisure 3(2):1–7Miranda-Gumucio L, Gil-Pechuán I, Palacios-Marqués D (2013) An exploratory study of the determinants of switching and loyalty in prepaid cell phone users. An application of concept mapping. Serv Bus 7(4):603–622Miret-Pastor L, Segarra-Oña MV (2011) Estudio del clúster turístico de Benidorm a través de indicadores de aglomeración y especialización. Renovación de destinos turísticos consolidados, pp 69–86Miret-Pastor L, Segarra-Oña MV, Hervás-Oliver JL (2009) Un análisis sobre la concentración espacial en el turismo valenciano. Congreso de la Asociación Española de Ciencia Regional. XXXV Reunión de estudios regionales, ValenciaMoscardo G (2011) Exploring social representations of tourism planning: issues for governance. J Sustain Tour 19(4-5):423–436Muñoz-Cañavate A, Chaín Navarro C (2004) La administración local española en internet: estudio cuantitativo de la evolución de los sistemas de información web de los ayuntamientos (1997–2002). Ciencias de la Inf 35(1):43–55Nabitz U, Severens P, Brink WVD, Jansen P (2007) Improving the EFQM model: an empirical study on model development and theory building using concept mapping. Total Qual Manag 12(1):69–81National and Integral Tourism Plan (2012–2015) http://www.minetur.gob.es/turismo/es-ES/PNIT/Documents/Plan%20Nacional%20e%20Integral%20de%20Turismo%20(PNIT)%202012-2015.pdf . Consulted in 2016Nicholson J, Orr K (2016) Local government partnership working: a space odyssey. Or, journeys through the dilemmas of public and private sector boundary-spanning actors. Policy Politics 44(2):269–287Nordin S, Svensson B (2007) Innovative destination governance: the Swedish ski resort of Åre. Entrepr Innov 8(1):53–66Nunkoo R (2015) Tourism development and trust in local government. Tour Manag 46:623–634Pechlaner H, Tschurtschenthaler P (2003) Tourism policy, tourism organisations and change management in Alpine regions and destinations: a european perspective. Curr Issues Tour 6(6):508–539Pfeffer J, Salancik G (1978) The external control of organizations: a resource-dependence perspective. Harper and Row, New YorkPorter ME (1990) The competitive advantage of nations. Harv Bus Rev 68(2):73–93Prahalad CK, Hamel G (1990) The core competence of the corporation. Harv Bus Rev 68(3):79–91Queiroz F, Rastrollo-Horrillo MA (2015) State of art in tourist destination governance. Tour Manag Stud 11(2):47–55. doi: 10.18089/tms.2015.11206Rodríguez MP, López AM, Ortiz D (2010) Implementing the balanced scorecard in public sector agencies: An experience in municipal sport services. Acad Rev latinoam de adm 45:116–139Rooter report (2010) La innovación en servicios en España. www.rooter.es . Consulted in 2016Rosas SR (2005) Concept mapping as a technique for program theory development: an illustration using family support programs. Am J Eval 26(3):389–401Rosas SR, Camphausen LC (2007) The use of concept mapping for scale development and validation in evaluation. Eval Progr Plann 30:125–135Santos M, Ferreira AM, Costa C (2014) Influential factors in the competitiveness of mature tourism destinations. Tour Manag Stud 10(1):73–81Selin S, Chavez D (1994) Characteristics of successful tourism partnerships: a multiple case study design. J Park Recreat Adm 12(2):51–61Selin S, Chavez D (1995) Developing an evolutionary tourism partnership model. Ann Tour Res 22(4):844–856Simpson B (1994) How do women scientists perceive their own career development? Int J Career Manag 6(1):19–27Soler-López MC (2013) Calidad y Rendimiento de Sitios Web de e-Government. Aplicación a la Administración Local. Doctoral thesis. Murcia UniversitySpanish Tourism Plan Horizon (2020) pp. 24 and 43. http://static.hosteltur.com/web/uploads/2011/10/Plan_de_Turismo_Espaol_Horizonte_2020.pdf . Consulted in 2016Toral SL, Barrero F, Martínez MR, Gallardo S, Cortés FJ (2006) Determinación de las variables de diseño en el desarrollo de una herramienta de e-learning. Pixel-Bit, Rev de medios y edu 27:99–113Torres L, Pina V (2001) Public–private partnership and private finance initiatives in the EU and Spanish local governments. Eur Account Rev 10:601–619Trochim WMK (1989) An introduction to concept mapping for planning and evaluation. Eval Progr Plann 12(1):1–16Trochim WMK, Linton R (1986) Conceptualization for planning and evaluation. Eval Progr Plann 9(4):289–308United Nations World Tourism Organization (2005) Making tourism more sustainable. A guide for policy makers, UNEP and UNWTO, pp 11–12. http://sdt.unwto.org/content/about-us-5United Nations World Tourism Organization (2015) UNWTO tourism highlights, p 6. http://www.e-unwto.org/doi/pdf/10.18111/9789284416899Velasco GM (2007) Gestión de destinos: ¿Gobernabilidad del turismo o gobernanza del destino?. Ministerio de educación y ciencia de España. Plan nacional de I + D + I (2004–2007)Wang Y (2008) Collaborative destination marketing: understanding the dynamic process. J Travel Res 47(2):151–166Wang Y, Fesenmaier RD (2007) Collaborative destination marketing: a case study of Elkhart county, Indiana. Tour Manag 28:863–875Wang C, Honggang X (2014) The role of local government and the private sector in China’s tourism industry. Tour Manag 45:95–105Williamson OE (1975) Markets and hierarchies: analysis and antitrust implications. The Free Press, New YorkWood DJ, Gray B (1991) Towards a comprehensive theory of collaboration. J Appl Behav Sci 27:139–162World Travel & Tourism Council (2016) Economic Impact 2016 Spain, p 3. http://www.wttc.org/-/media/files/reports/economic%20impact%20research/countries%202016/spain2016.pdf . Consulted in 2016Zapata MJ, Hall CM (2012) Public–private collaboration in the tourist sector: balancing legitimacy and effectiveness in local tourism partnerships. The Spanish case. J Policy Res Tour Leisure Events 4(1):61–8

Comparison of Promoter Hypermethylation Pattern in Salivary Rinses Collected with and without an Exfoliating Brush from Patients with HNSCC

Background: Salivary rinses have been recently proposed as a valuable resource for the development of epigenetic biomarkers for detection and monitoring of head and neck squamous cell carcinoma (HNSCC). Both salivary rinses collected with and without an exfoliating brush from patients with HNSCC are used in detection of promoter hypermethylation, yet their correlation of promoter hypermethylation has not been evaluated. This study was to evaluate the concordance of promoter hypermethylation between salivary rinses collected with and without an exfoliating brush from patients with HNSCC. Methodolgy: 57 paired salivary rinses collected with or without an exfoliating brush from identical HNSCC patients were evaluated for promoter hypermethylation status using Quantitative Methylation-Specific PCR. Target tumor suppressor gene promoter regions were selected based on our previous studies describing a panel for HNSCC screening an

Integrin CD11b positively regulates TLR4-induced signalling pathways in dendritic cells but not in macrophages.

Tuned and distinct responses of macrophages and dendritic cells to Toll-like receptor 4 (TLR4) activation induced by lipopolysaccharide (LPS) underpin the balance between innate and adaptive immunity. However, the molecule(s) that confer these cell-type-specific LPS-induced effects remain poorly understood. Here we report that the integrin α(M) (CD11b) positively regulates LPS-induced signalling pathways selectively in myeloid dendritic cells but not in macrophages. In dendritic cells, which express lower levels of CD14 and TLR4 than macrophages, CD11b promotes MyD88-dependent and MyD88-independent signalling pathways. In particular, in dendritic cells CD11b facilitates LPS-induced TLR4 endocytosis and is required for the subsequent signalling in the endosomes. Consistent with this, CD11b deficiency dampens dendritic cell-mediated TLR4-triggered responses in vivo leading to impaired T-cell activation. Thus, by modulating the trafficking and signalling functions of TLR4 in a cell-type-specific manner CD11b fine tunes the balance between adaptive and innate immune responses initiated by LPS

Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma

<p>Abstract</p> <p>Background</p> <p>The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiation. Aberrant expression of UCHL1 has been found in a number of human solid tumors including renal cell carcinoma (RCC). In RCC, UCHL1 overexpression is associated with tumor progression and an altered von Hippel Lindau gene expression.</p> <p>Methods</p> <p>To determine the underlying mechanisms for the heterogeneous UCHL1 expression pattern in RCC the UCHL1 promoter DNA methylation status was determined in 17 RCC cell lines as well as in 32 RCC lesions and corresponding tumor adjacent kidney epithelium using combined bisulfite restriction analysis as well as bisulfite DNA sequencing.</p> <p>Results</p> <p>UCHL1 expression was found in all 32 tumor adjacent kidney epithelium samples. However, the lack of or reduced UCHL1 mRNA and/or protein expression was detected in 13/32 RCC biopsies and 7/17 RCC cell lines and due to either a total or partial methylation of the UCHL1 promoter DNA. Upon 2'-deoxy-5-azacytidine treatment an induction of UCHL1 mRNA and protein expression was found in 9/17 RCC cell lines, which was linked to the demethylation degree of the UCHL1 promoter DNA.</p> <p>Conclusion</p> <p>Promoter hypermethylation represents a mechanism for the silencing of the UCHL1 gene expression in RCC and supports the concept of an epigenetic control for the expression of UCHL1 during disease progression.</p

Methylation profiling of twenty promoter-CpG islands of genes which may contribute to hepatocellular carcinogenesis

BACKGROUND: Hepatocellular carcinoma (HCC) presents one of the major health threats in China today. A better understanding of the molecular genetics underlying malignant transformation of hepatocytes is critical to success in the battle against this disease. The methylation state of C5 of the cytosine in the CpG di-nucleotide that is enriched within or near the promoter region of over 50 % of the polymerase II genes has a drastic effect on transcription of these genes. Changes in the methylation profile of the promoters represent an alternative to genetic lesions as causative factors for the tumor-specific aberrant expression of the genes. METHODS: We have used the methylation specific PCR method in conjunction with DNA sequencing to assess the methylation state of the promoter CpG islands of twenty genes. Aberrant expression of these genes have been attributed to the abnormal methylation profile of the corresponding promoter CpG islands in human tumors. RESULTS: While the following sixteen genes remained the unmethylated in all tumor and normal tissues: CDH1, APAF1, hMLH1, BRCA1, hTERC, VHL, RARβ, TIMP3, DAPK1, SURVIVIN, p14(ARF), RB1, p15(INK4b), APC, RASSF1c and PTEN, varying degrees of tumor specific hypermethylation were associated with the p16(INK4a ), RASSF1a, CASP8 and CDH13 genes. For instance, the p16(INK4a )was highly methylated in HCC (17/29, 58.6%) and less significantly methylated in non-cancerous tissue (4/29. 13.79%). The RASSF1a was fully methylated in all tumor tissues (29/29, 100%), and less frequently methylated in corresponding non-cancerous tissue (24/29, 82.75%). CONCLUSIONS: Furthermore, co-existence of methylated with unmethylated DNA in some cases suggested that both genetic and epigenetic (CpG methylation) mechanisms may act in concert to inactivate the p16(INK4a )and RASSF1a in HCC. Finally, we found a significant association of cirrhosis with hypermethylation of the p16(INK4a )and hypomethylation of the CDH13 genes. For the first time, the survey was carried out on such an extent that it would not only provide new insights into the molecular mechanisms underscoring the aberrant expression of the genes in this study in HCC, but also offer essential information required for a good methylation-based diagnosis of HCC

Detection and Early Referral of Patients With Interstitial Lung Abnormalities: An Expert Survey Initiative

Background: Interstitial lung abnormalities (ILA) may represent undiagnosed early-stage or subclinical interstitial lung disease (ILD). ILA are often observed incidentally in patients who subsequently develop clinically overt ILD. There is limited information on consensus definitions for, and the appropriate evaluation of, ILA. Early recognition of patients with ILD remains challenging, yet critically important. Expert consensus could inform early recognition and referral. Research Question: Can consensus-based expert recommendations be identified to guide clinicians in the recognition, referral, and follow-up of patients with or at risk of developing early ILDs? Study Design and Methods: Pulmonologists and radiologists with expertise in ILD participated in two iterative rounds of surveys. The surveys aimed to establish consensus regarding ILA reporting, identification of patients with ILA, and identification of populations that might benefit from screening for ILD. Recommended referral criteria and follow-up processes were also addressed. Threshold for consensus was defined a priori as ≥ 75% agreement or disagreement. Results: Fifty-five experts were invited and 44 participated; consensus was reached on 39 of 85 questions. The following clinically important statements achieved consensus: honeycombing and traction bronchiectasis or bronchiolectasis indicate potentially progressive ILD; honeycombing detected during lung cancer screening should be reported as potentially significant (eg, with the Lung CT Screening Reporting and Data System “S-modifier” [Lung-RADS; which indicates clinically significant or potentially significant noncancer findings]), recommending referral to a pulmonologist in the radiology report; high-resolution CT imaging and full pulmonary function tests should be ordered if nondependent subpleural reticulation, traction bronchiectasis, honeycombing, centrilobular ground-glass nodules, or patchy ground-glass opacity are observed on CT imaging; patients with honeycombing or traction bronchiectasis should be referred to a pulmonologist irrespective of diffusion capacity values; and patients with systemic sclerosis should be screened with pulmonary function tests for early-stage ILD. Interpretation: Guidance was established for identifying clinically relevant ILA, subsequent referral, and follow-up. These results lay the foundation for developing practical guidance on managing patients with ILA