Sequential optimization method for the design of electromagnetic device

Three sequential optimization methods, sequential least square method, sequential Kriging method, and sequential linear Bayesian method, are presented for the optimization design of electromagnetic device. Sequential optimization method (SOM) is composed of coarse optimization process and fine optimization process. The main purpose of the former is to reduce the design space; while the target of the latter is to update the optimal design parameters. To illustrate the performance of the proposed methods, an analytic test function and the TEAM Workshop Problem 22 are investigated. Experimental results of test function demonstrate that SOM can obtain satisfactory solutions; and practical application illustrates that the number of finite element sample points is less than 1/10 compared with that by direct optimization method, while the optimal results are even better than that by direct optimization method. © 2008 IEEE

Electromagnetic device design based on RBF models and two new sequential optimization strategies

We present two new strategies for sequential optimization method (SOM) to deal with the optimization design problems of electromagnetic devices. One is a new space reduction strategy; the other is model selection strategy. Meanwhile, radial basis function (RBF) and compactly supported RBF models are investigated to extend the applied model types for SOM. Thereafter, Monte Carlo method is employed to demonstrate the efficiency and superiority of the new space reduction strategy. Five commonly used approximate models are considered for the discussion of model selection strategy. Furthermore, by two TEAM benchmark examples, we can see that SOM with the proposed new strategies and models can significantly speed the optimization design process, and the efficiency of SOM depends a little on the types of approximate models. © 2006 IEEE

Domain decomposition combined radial basis function collocation method to solve transient eddy current magnetic problems with moving conductors

Radial basis function (RBF) collocation method is a kind of pure meshless numerical method and has been applied to solve static and transient electromagnetic problems. Especially, it shows a great advantage in the computation of moving conductor eddy current magnetic problems. To simulate the conductor movement, the field equations are decoupled with superposition principle and solved by time-domain iteration under moving coordinate systems. One problem is that the coefficient matrix of RBF governing equations, which needs to be computed in each iteration step, is full. As the number of RBF nodes increases, the computational capacity will grow rapidly. The domain decomposition method (DDM), which divides the solving domain into several subdomains, could be conveniently combined with RBF collocation method. This paper first applies DDM combined RBF collocation method to compute transient eddy current magnetic field problems with moving conductors. With this novel method, the iteration only proceeds in the subdomains containing conductors. And the magnetic field in the subdomains without conductors needs to be computed just once before the iteration. The dimension of the coefficient matrix computed in the iteration is only determined by the number of nodes in the corresponding subdomains and on the interfaces. An engineering problem is computed to show that the DDM combined RBF collocation method is much more efficient than the normal one. © 2011 IEEE

A novel superposition RBF collocation method to solve moving conductor eddy current problems

This paper presents a novel radial basis function (RBF) collocation method to solve the moving conductor eddy current problem. The magnetic field is considered an addition of two fields generated respectively by the excitation current and the eddy current according to the source superposition principle. The corresponding governing equations are decoupled and solved with the RBF. Moving coordinate systems in which the separate fields are computed are also constructed to avoid the model reconfiguration caused by the motion. Electromagnetic field equations are analyzed with kinetic equations and circuit equations together to simulate the motion process. A practical engineering problem is computed to verify the method. © 2009 IEEE

Why are MD simulated protein folding times wrong?

The question of significant deviations of protein folding times simulated using molecular dynamics from experimental values is investigated. It is shown that in the framework of Markov State Model (MSM) describing the conformational dynamics of peptides and proteins, the folding time is very sensitive to the simulation model parameters, such as forcefield and temperature. Using two peptides as examples, we show that the deviations in the folding times can reach an order of magnitude for modest variations of the molecular model. We, therefore, conclude that the folding rate values obtained in molecular dynamics simulations have to be treated with care

Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting

Aims: To compare the constitutive and agonist-dependent endosomal trafficking of α1A- and α1B-adrenoceptors (ARs) and to establish if the internalization pattern determines the signaling pathways of each subtype. Methods: Using CypHer5 technology and VSV-G epitope tagged α1A- and α1B-ARs stably and transiently expressed in HEK 293 cells, we analyzed by confocal microscopy the constitutive and agonist-induced internalization of each subtype, and the temporal relationship between agonist induced internalization and the increase in intracellular calcium (determined by FLUO-3 flouorescence), or the phosphorylation of ERK1/2 and p38 MAP kinases (determined by Western blot). Results and Conclusions: Constitutive as well as agonist-induced trafficking of α1A and α1B ARs maintain two different endosomal pools of receptors: one located close to the plasma membrane and the other deeper into the cytosol. Each subtype exhibited specific characteristics of internalization and distribution between these pools that determines their signaling pathways: α1A-ARs, when located in the plasma membrane, signal through calcium and ERK1/2 pathways but, when translocated to deeper endosomes, through a mechanism sensitive to β-arrestin and concanavalin A, continue signaling through ERK1/2 and also activate the p38 pathway. α1B-ARs signal through calcium and ERK1/2 only when located in the membrane and the signals disappear after endocytosis and by disruption of the membrane lipid rafts by methyl-β-cyclodextrin

30 inch Roll-Based Production of High-Quality Graphene Films for Flexible Transparent Electrodes

We report that 30-inch scale multiple roll-to-roll transfer and wet chemical doping considerably enhance the electrical properties of the graphene films grown on roll-type Cu substrates by chemical vapor deposition. The resulting graphene films shows a sheet resistance as low as ~30 Ohm/sq at ~90 % transparency which is superior to commercial transparent electrodes such as indium tin oxides (ITO). The monolayer of graphene shows sheet resistances as low as ~125 Ohm/sq with 97.4% optical transmittance and half-integer quantum Hall effect, indicating the high-quality of these graphene films. As a practical application, we also fabricated a touch screen panel device based on the graphene transparent electrodes, showing extraordinary mechanical and electrical performances

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.

We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these results, we anticipate that cellular context will be critical to synthetic-lethal therapies

A Widespread Distribution of Genomic CeMyoD Binding Sites Revealed and Cross Validated by ChIP-Chip and ChIP-Seq Techniques

Identifying transcription factor binding sites genome-wide using chromatin immunoprecipitation (ChIP)-based technology is becoming an increasingly important tool in addressing developmental questions. However, technical problems associated with factor abundance and suitable ChIP reagents are common obstacles to these studies in many biological systems. We have used two completely different, widely applicable methods to determine by ChIP the genome-wide binding sites of the master myogenic regulatory transcription factor HLH-1 (CeMyoD) in C. elegans embryos. The two approaches, ChIP-seq and ChIP-chip, yield strongly overlapping results revealing that HLH-1 preferentially binds to promoter regions of genes enriched for E-box sequences (CANNTG), known binding sites for this well-studied class of transcription factors. HLH-1 binding sites were enriched upstream of genes known to be expressed in muscle, consistent with its role as a direct transcriptional regulator. HLH-1 binding was also detected at numerous sites unassociated with muscle gene expression, as has been previously described for its mouse homolog MyoD. These binding sites may reflect several additional functions for HLH-1, including its interactions with one or more co-factors to activate (or repress) gene expression or a role in chromatin organization distinct from direct transcriptional regulation of target genes. Our results also provide a comparison of ChIP methodologies that can overcome limitations commonly encountered in these types of studies while highlighting the complications of assigning in vivo functions to identified target sites

Photovoltaic restoration of sight with high visual acuity

Patients with retinal degeneration lose sight due to the gradual demise of photoreceptors. Electrical stimulation of surviving retinal neurons provides an alternative route for the delivery of visual information. We demonstrate that subretinal implants with 70-μm-wide photovoltaic pixels provide highly localized stimulation of retinal neurons in rats. The electrical receptive fields recorded in retinal ganglion cells were similar in size to the natural visual receptive fields. Similarly to normal vision, the retinal response to prosthetic stimulation exhibited flicker fusion at high frequencies, adaptation to static images and nonlinear spatial summation. In rats with retinal degeneration, these photovoltaic arrays elicited retinal responses with a spatial resolution of 64 ± 11 μm, corresponding to half of the normal visual acuity in healthy rats. The ease of implantation of these wireless and modular arrays, combined with their high resolution, opens the door to the functional restoration of sight in patients blinded by retinal degeneration