Exploring Bovine Pancreatic Trypsin Inhibitor Phase Transitions.

This paper presents an investigation of the phase diagram of BPTI ( bovine pancreatic trypsin inhibitor)/350 mM KSCN at pH 4.9 by direct observation and numerical simulations. We report optical microscopy and light and X-ray scattering experiments coupled with theoretical data analysis using numerical tools. The phase diagram is thoroughly determined, as a function of temperature. Two polymorphs are observed by video microscopy and their solubility measured. In this phase diagram, the liquid-liquid phase separation (LLPS) is metastable with respect to the solid-liquid phase separation. Above the TL-L boundary curve, solutions are composed of a mixture of BPTI monomers and decamers. Attractive interactions are stronger between decamers than between monomers. Below the TL-L boundary curve, the dense phase is highly concentrated in protein and composed of BPTI decamers alone. Thus, the driving force for liquid-liquid or liquid-solid phase separation is the attraction between decamers at low pH. The structure factors of the dense phases are characteristic of repulsive dense phases because of a hard sphere repulsion core, meaning that in the dense phase proteins are actually in contact (interparticle distance of 53 angstrom). In agreement with the Oswald rule of stages, LLPS occurs prior to and impedes the solid nucleation

Dark Energy Survey Year 3 results: Cosmology with moments of weak lensing mass maps

We present a cosmological analysis using the second and third moments of the weak lensing mass (convergence) maps from the first three years of data (Y3) data of the Dark Energy Survey. The survey spans an effective area of 4139 square degrees and uses the images of over 100 million galaxies to reconstruct the convergence field. The second moment of the convergence as a function of smoothing scale contains information similar to standard shear 2-point statistics. The third moment, or the skewness, contains additional non-Gaussian information. The data is analyzed in the context of the ΛCDM model, varying five cosmological parameters and 19 nuisance parameters modeling astrophysical and measurement systematics. Our modeling of the observables is completely analytical, and has been tested with simulations in our previous methodology study. We obtain a 1.7% measurement of the amplitude of fluctuations parameter S8≡σ8(Ωm/0.3)0.5=0.784±0.013. The measurements are shown to be internally consistent across redshift bins, angular scales, and between second and third moments. In particular, the measured third moment is consistent with the expectation of gravitational clustering under the ΛCDM model. The addition of the third moment improves the constraints on S8 and Ωm by ∼15% and ∼25% compared to an analysis that only uses second moments. We compare our results with Planck constraints from the cosmic microwave background, finding a 2.2–2.8σ tension in the full parameter space, depending on the combination of moments considered. The third moment, independently, is in 2.8σ tension with Planck, and thus provides a cross-check on the analyses of 2-point correlations