1,957 research outputs found
Characterizing Width Uniformity by Wave Propagation
This work describes a novel image analysis approach to characterize the
uniformity of objects in agglomerates by using the propagation of normal
wavefronts. The problem of width uniformity is discussed and its importance for
the characterization of composite structures normally found in physics and
biology highlighted. The methodology involves identifying each cluster (i.e.
connected component) of interest, which can correspond to objects or voids, and
estimating the respective medial axes by using a recently proposed wavefront
propagation approach, which is briefly reviewed. The distance values along such
axes are identified and their mean and standard deviation values obtained. As
illustrated with respect to synthetic and real objects (in vitro cultures of
neuronal cells), the combined use of these two features provide a powerful
description of the uniformity of the separation between the objects, presenting
potential for several applications in material sciences and biology.Comment: 14 pages, 23 figures, 1 table, 1 referenc
Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons
Inflammation and oxidative stress are common aspects of most neurodegenerative diseases in the central nervous system. In this context, microglia and astrocytes are central to mediating the balance between neuroprotective and neurodestructive mechanisms. Flavonoids have potent anti-inflammatory and antioxidant properties. Here, we have examined the anti-inflammatory and neuroprotective potential of the flavonoid agathisflavone (FAB), which is derived from the Brazilian plant Poincianella pyramidalis, in in vitro models of neuroinflammation. Cocultures of neurons/glial cells were exposed to lipopolysaccharide (LPS, 1 µg/mL) or interleukin (IL)-1β (10 ng/mL) for 24 h and treated with FAB (0.1 and 1 µM, 24 h). FAB displayed a significant neuroprotective effect, as measured by nitric oxide (NO) production, Fluoro-Jade B (FJ-B) staining, and immunocytochemistry (ICC) for the neuronal marker β-tubulin and the cell death marker caspase-3, preserving neuronal soma and increasing neurite outgrowth. FAB significantly decreased the LPS-induced microglial proliferation, identified by ICC for Iba-1/bromodeoxyuridine (BrdU) and CD68 (microglia M1 profile marker). In contrast, FAB had no apparent effect on astrocytes, as determined by ICC for glial fibrillary acidic protein (GFAP). Furthermore, FAB protected against the cytodestructive and proinflammatory effects of IL-1β, a key cytokine that is released by activated microglia and astrocytes, and ICC showed that combined treatment of FAB with α and β estrogen receptor antagonists did not affect NF-κB expression. In addition, qPCR analysis demonstrated that FAB decreased the expression of proinflammatory molecules TNF-α, IL-1β, and connexins CCL5 and CCL2, as well as increased the expression of the regulatory molecule IL-10. Together, these findings indicate that FAB has a significant neuroprotective and anti-inflammatory effect in vitro, which may be considered as an adjuvant for the treatment of neurodegenerative diseases
The flavonoid agathisflavone modulates the microglial neuroinflammatory response and enhances remyelination
Myelin loss is the hallmark of the demyelinating disease multiple sclerosis (MS) and plays a significant role in multiple neurodegenerative diseases. A common factor in all neuropathologies is the central role of microglia, the intrinsic immune cells of the central nervous system (CNS). Microglia are activated in pathology and can have both pro- and anti-inflammatory functions. Here, we examined the effects of the flavonoid agathisflavone on microglia and remyelination in the cerebellar slice model following lysolecithin induced demyelination. Notably, agathisflavone enhances remyelination and alters microglial activation state, as determined by their morphology and cytokine profile. Furthermore, these effects of agathisflavone on remyelination and microglial activation were inhibited by blockade of estrogen receptor α. Thus, our results identify agathisflavone as a novel compound that may act via ER to regulate microglial activation and enhance remyelination and repair
Accurate Atmospheric Parameters at Moderate Resolution Using Spectral Indices: Preliminary Application to the MARVELS Survey
Studies of Galactic chemical and dynamical evolution in the solar
neighborhood depend on the availability of precise atmospheric parameters
(Teff, [Fe/H] and log g) for solar-type stars. Many large-scale spectroscopic
surveys operate at low to moderate spectral resolution for efficiency in
observing large samples, which makes the stellar characterization difficult due
to the high degree of blending of spectral features. While most surveys use
spectral synthesis, in this work we employ an alternative method based on
spectral indices to determine the atmospheric parameters of a sample of nearby
FGK dwarfs and subgiants observed by the MARVELS survey at moderate resolving
power (R~12,000). We have developed three codes to automatically normalize the
observed spectra, measure the equivalent widths of the indices and, through the
comparison of those with values calculated with pre-determined calibrations,
derive the atmospheric parameters of the stars. The calibrations were built
using a sample of 309 stars with precise stellar parameters obtained from the
analysis of high-resolution FEROS spectra. A validation test of the method was
conducted with a sample of 30 MARVELS targets that also have reliable
atmospheric parameters from high-resolution spectroscopic analysis. Our
approach was able to recover the parameters within 80 K for Teff, 0.05 dex for
[Fe/H] and 0.15 dex for log g, values that are lower or equal to the typical
external uncertainties found between different high-resolution analyzes. An
additional test was performed with a subsample of 138 stars from the ELODIE
stellar library and the literature atmospheric parameters were recovered within
125 K for Teff, 0.10 dex for [Fe/H] and 0.29 dex for log g. These results show
that the spectral indices are a competitive tool to characterize stars with the
intermediate resolution spectra.Comment: Accepted for publication in AJ. Abstract edited to comply with arXiv
standards regarding the number of character
Exact Diagonalization of Two Quantum Models for the Damped Harmonic Oscillator
The damped harmonic oscillator is a workhorse for the study of dissipation in
quantum mechanics. However, despite its simplicity, this system has given rise
to some approximations whose validity and relation to more refined descriptions
deserve a thorough investigation. In this work, we apply a method that allows
us to diagonalize exactly the dissipative Hamiltonians that are frequently
adopted in the literature. Using this method we derive the conditions of
validity of the rotating-wave approximation (RWA) and show how this approximate
description relates to more general ones. We also show that the existence of
dissipative coherent states is intimately related to the RWA. Finally, through
the evaluation of the dynamics of the damped oscillator, we notice an important
property of the dissipative model that has not been properly accounted for in
previous works; namely, the necessity of new constraints to the application of
the factorizable initial conditions.Comment: 19 pages, 2 figures, ReVTe
PCR-based identification of Neospora caninum in the umbilical cord of a newborn calf in Brazil
Double Inflation in Supergravity and the Large Scale Structure
The cosmological implication of a double inflation model with hybrid + new
inflations in supergravity is studied. The hybrid inflation drives an inflaton
for new inflation close to the origin through supergravity effects and new
inflation naturally occurs. If the total e-fold number of new inflation is
smaller than , both inflations produce cosmologically relevant density
fluctuations. Both cluster abundances and galaxy distributions provide strong
constraints on the parameters in the double inflation model assuming
standard cold dark matter scenario. The future satellite
experiments to measure the angular power spectrum of the cosmic microwave
background will make a precise determination of the model parameters possible.Comment: 19 pages (RevTeX file
Possibilidade da transmissão congênita de Toxoplasma gondii em ovinos através de seguimento sorológico no município de Rosário do Sul, RS, Brasil
Cosmic Microwave Background Anisotropies from Scaling Seeds: Global Defect Models
We investigate the global texture model of structure formation in cosmogonies
with non-zero cosmological constant for different values of the Hubble
parameter. We find that the absence of significant acoustic peaks and little
power on large scales are robust predictions of these models. However, from a
careful comparison with data we conclude that at present we cannot safely
reject the model on the grounds of present CMB data. Exclusion by means of
galaxy correlation data requires assumptions on biasing and statistics. New,
very stringent constraints come from peculiar velocities.
Investigating the large-N limit, we argue that our main conclusions apply to
all global O(N) models of structure formation.Comment: LaTeX file with RevTex, 27 pages, 23 eps figs., submitted to Phys.
Rev. D. A version with higher quality images can be found at
http://mykonos.unige.ch/~kunz/download/lam.tar.gz for the LaTeX archive and
at http://mykonos.unige.ch/~kunz/download/lam.ps.gz for the compiled
PostScript fil
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