PADRONIZAÇÃO DA CITOLOGIA DE IMPRESSÃO DA SUPERFÍCIE OCULAR CANINA

Técnica de exame de citologia de impressão foi padronizada em olhos de cães sem alterações oculares. Foram realizados exames de citologia de impressão do epitélio corneano, conjuntival e tarsal em 30 olhos de 21 animais de raças e idades variadas. As amostras foram colhidas de cães atendidos no Hospital Veterinário da FMVZ-USP entre fevereiro e julho de 2003, sendo coradas e avaliadas no Laboratório de Doenças Externas Oculares da UNIFESP. A colheita foi bem tolerada pelos cães e o papel filtro utilizado removeu células em quantidade e morfologia adequadas para estudo citológico. Foi observado em 100% dos casos que o epitélio da conjuntiva bulbar canina apresenta aspecto metaplasia-like, com ausência de células caliciformes. Estas só foram encontradas na conjuntiva tarsal em 21,4% das amostras avaliadas dessa região. A citologia de impressão é um método factível para avaliação da superfície ocular em cães. Entretanto, a celularidade das amostras obtidas do tarso mostrou-se inadequada. Além disso, a pesquisa da densidade de células caliciformes em áreas bulbares, embora usada em seres humanos, pode não servir como indicador de alteração da superfície ocular para a espécie canina. Standardization of canine ocular surface impression cytology Abstract Impression cytology technique in dog eyes without ocular disease was standardized. Impression cytology was performed in corneal, conjunctival and tarsal epithelium in 30 eyes of 21 animals with different races and ages. Samples were obtained from dogs attended in FMVZ-USP Veterinary Hospital between February to July 2003, being stained and evaluated at UNIFESP´s External Eye Disease Laboratory. Sampling was well tolerated by dogs and the filter paper used removed cells with adequate morphology and quantity for cytologyc evaluation. In all cases canine bulbar conjunctival epithelium showed metaplasia-like features without goblet cells. Impression cytology is a feasible method for ocular surface evaluation in dogs. However, celularity was considered inadequated in samples obtained from tarsal conjunctiva. Furthermore, seeking goblet cell density in bulbar areas, although used in human beings, may not be used as an ocular surface disease indicator in canine species

Direct and Indirect Detection of Dark Matter in D6 Flavor Symmetric Model

We study a fermionic dark matter in a non-supersymmetric extension of the standard model with a family symmetry based on D6xZ2xZ2. In our model, the final state of the dark matter annihilation is determined to be e+ e- by the flavor symmetry, which is consistent with the PAMELA result. At first, we show that our dark matter mass should be within the range of 230 GeV - 750 GeV in the WMAP analysis combined with mu to e gamma constraint. Moreover we simultaneously explain the experiments of direct and indirect detection, by simply adding a gauge and D6 singlet real scalar field. In the direct detection experiments, we show that the lighter dark matter mass ~ 230 GeV and the lighter standard model Higgs boson ~ 115 GeV is in favor of the observed bounds reported by CDMS II and XENON100. In the indirect detection experiments, we explain the positron excess reported by PAMELA through the Breit-Wigner enhancement mechanism. We also show that our model is consistent with no antiproton excess suggested by PAMELA.Comment: 20 pages, 9 figures, 2 tables, accepted version for publication in European Physical Journal

What if Supersymmetry Breaking Unifies beyond the GUT Scale?

We study models in which soft supersymmetry-breaking parameters of the MSSM become universal at some unification scale, $M_{in}$, above the GUT scale, \mgut. We assume that the scalar masses and gaugino masses have common values, $m_0$ and $m_{1/2}$ respectively, at $M_{in}$. We use the renormalization-group equations of the minimal supersymmetric SU(5) GUT to evaluate their evolutions down to \mgut, studying their dependences on the unknown parameters of the SU(5) superpotential. After displaying some generic examples of the evolutions of the soft supersymmetry-breaking parameters, we discuss the effects on physical sparticle masses in some specific examples. We note, for example, that near-degeneracy between the lightest neutralino and the lighter stau is progressively disfavoured as $M_{in}$ increases. This has the consequence, as we show in $(m_{1/2}, m_0)$ planes for several different values of $\tan \beta$, that the stau coannihilation region shrinks as $M_{in}$ increases, and we delineate the regions of the $(M_{in}, \tan \beta)$ plane where it is absent altogether. Moreover, as $M_{in}$ increases, the focus-point region recedes to larger values of $m_0$ for any fixed $\tan \beta$ and $m_{1/2}$. We conclude that the regions of the $(m_{1/2}, m_0)$ plane that are commonly favoured in phenomenological analyses tend to disappear at large $M_{in}$.Comment: 24 pages with 11 eps figures; references added, some figures corrected, discussion extended and figure added; version to appear in EPJ

Polymorphism: an evaluation of the potential risk to the quality of drug products from the Farmácia Popular Rede Própria

Polymorphism in solids is a common phenomenon in drugs, which can lead to compromised quality due to changes in their physicochemical properties, particularly solubility, and, therefore, reduce bioavailability. Herein, a bibliographic survey was performed based on key issues and studies related to polymorphism in active pharmaceutical ingredient (APIs) present in medications from the Farmácia Popular Rede Própria. Polymorphism must be controlled to prevent possible ineffective therapy and/or improper dosage. Few mandatory tests for the identification and control of polymorphism in medications are currently available, which can result in serious public health concerns

PADRONIZAÇÃO DA CITOLOGIA DE IMPRESSÃO DA SUPERFÍCIE OCULAR CANINA

Técnica de exame de citologia de impressão foi padronizada em olhos de cães sem alterações oculares. Foram realizados exames de citologia de impressão do epitélio corneano, conjuntival e tarsal em 30 olhos de 21 animais de raças e idades variadas. As amostras foram colhidas de cães atendidos no Hospital Veterinário da FMVZ-USP entre fevereiro e julho de 2003, sendo coradas e avaliadas no Laboratório de Doenças Externas Oculares da UNIFESP. A colheita foi bem tolerada pelos cães e o papel filtro utilizado removeu células em quantidade e morfologia adequadas para estudo citológico. Foi observado em 100% dos casos que o epitélio da conjuntiva bulbar canina apresenta aspecto metaplasia-like, com ausência de células caliciformes. Estas só foram encontradas na conjuntiva tarsal em 21,4% das amostras avaliadas dessa região. A citologia de impressão é um método factível para avaliação da superfície ocular em cães. Entretanto, a celularidade das amostras obtidas do tarso mostrou-se inadequada. Além disso, a pesquisa da densidade de células caliciformes em áreas bulbares, embora usada em seres humanos, pode não servir como indicador de alteração da superfície ocular para a espécie canina. Standardization of canine ocular surface impression cytology Abstract Impression cytology technique in dog eyes without ocular disease was standardized. Impression cytology was performed in corneal, conjunctival and tarsal epithelium in 30 eyes of 21 animals with different races and ages. Samples were obtained from dogs attended in FMVZ-USP Veterinary Hospital between February to July 2003, being stained and evaluated at UNIFESP´s External Eye Disease Laboratory. Sampling was well tolerated by dogs and the filter paper used removed cells with adequate morphology and quantity for cytologyc evaluation. In all cases canine bulbar conjunctival epithelium showed metaplasia-like features without goblet cells. Impression cytology is a feasible method for ocular surface evaluation in dogs. However, celularity was considered inadequated in samples obtained from tarsal conjunctiva. Furthermore, seeking goblet cell density in bulbar areas, although used in human beings, may not be used as an ocular surface disease indicator in canine species

Dual light and temperature responsive cotton fabric functionalized with a surface-grafted spiropyran-NIPAAm-hydrogel

A dual-responsive cotton fabric functionalized with a spiropyran-NIPAAm hydrogel, capable of dimensional changes upon irradiation with visible light or upon a temperature stimulus is reported. These volume changes are due to absorption and release of water, from and into the air, by increasing temperature above the LCST in the dark, and/or by irradiation with sunlight or white light from artificial sources. The material was obtained via grafting photo- and temperature-responsive monomers directly from the cotton fibers, using a facile controlled polymerization method, ARGET ATRP