Low thrust propulsion in a coplanar circular restricted four body problem

This paper formulates a circular restricted four body problem (CRFBP), where the three primaries are set in the stable Lagrangian equilateral triangle configuration and the fourth body is massless. The analysis of this autonomous coplanar CRFBP is undertaken, which identies eight natural equilibria; four of which are close to the smaller body, two stable and two unstable, when considering the primaries to be the Sun and two smaller bodies of the solar system. Following this, the model incorporates `near term' low-thrust propulsion capabilities to generate surfaces of articial equilibrium points close to the smaller primary, both in and out of the plane containing the celestial bodies. A stability analysis of these points is carried out and a stable subset of them is identied. Throughout the analysis the Sun-Jupiter-Asteroid-Spacecraft system is used, for conceivable masses of a hypothetical asteroid set at the libration point L4. It is shown that eight bounded orbits exist, which can be maintained with a constant thrust less than 1:5 10􀀀4N for a 1000kg spacecraft. This illustrates that, by exploiting low-thrust technologies, it would be possible to maintain an observation point more than 66% closer to the asteroid than that of a stable natural equilibrium point. The analysis then focusses on a major Jupiter Trojan: the 624-Hektor asteroid. The thrust required to enable close asteroid observation is determined in the simplied CRFBP model. Finally, a numerical simulation of the real Sun-Jupiter-624 Hektor-Spacecraft is undertaken, which tests the validity of the stability analysis of the simplied model

Biomedical and therapeutic applications of biosurfactants

During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases and as therapeutic agents due to their antibacterial, antifungal and antiviral activities. Furthermore, their role as anti-adhesive agents against several pathogens illustrate their utility as suitable anti-adhesive coating agents for medical insertional materials leading to a reduction of a large number of hospital infections without the use of synthetic drugs and chemicals. Biomedical and therapeutic perspectives of biosurfactants applications are presented and discussed in this chapter

Fungal Communities Resist Recovery in Sand Mine Restoration

The restoration of vegetation post-mining is particularly challenging in extreme conditions such as Mediterranean systems where soil moisture is limiting, soil temperature fluctuates dramatically, and soil carbon is very low. In such systems, soil microbial communities may play an important role in attenuating extreme conditions. Thus, vegetation establishment on such sites may be curtailed by depauperate soil communities. Soil fungal communities, in particular, are essential for nutrient turn over but we know very little about how these communities respond to mining and post-mining restoration. Fungi may be significantly affected by restoration practices. For example, the inclusion of deeper soil profiles (i.e., “overburden”) into restoration events is rare, but may expedite fungal community development. We studied a successional gradient of sand mine restoration in a former Banksia woodland in SW Australia to determine whether soil fungal communities recovered after 13 years. We also asked whether the inclusion of overburden into restoration sites improved soil fungal community development. Overall, fungal communities did not return to a pre-disturbance state by 13 years, nor did the inclusion of overburden affect their trajectory. Longer term studies are need to determine when, if ever, fungal communities are restored, and what effect this has nascent vegetation

Effluent decontamination by the ibuprofen-mineralizing strain, Sphingopyxis granuli RW412: Metabolic processes

Ibuprofen mineralization by Sphingopyxis granuli RW412 was characterized molecularly and the strain assayed to remediate ibuprofen from different artificially polluted media.The high global consumption of ibuprofen and its limited elimination by wastewater treatment plants (WWTPs), has led to the contamination of aquatic systems by this common analgesic and its metabolites. The potentially negative environmental and public health effects of this emerging contaminant have raised concerns, driving the demand for treatment technologies. The implementation of bacteria which mineralize organic contaminants in biopurification systems used to decontaminate water or directly in processes in WWTPs, is a cheap and sustainable means for complete elimination before release into the environment. In this work, an ibuprofen-mineralizing bacterial strain isolated from sediments of the River Elbe was characterized and assayed to remediate different ibuprofen-polluted media. Strain RW412, which was identified as Sphingopyxis granuli, has a 4.48 Mb genome which includes plasmid sequences which harbor the ipf genes that encode the first steps of ibuprofen mineralization. Here, we confirm that these genes encode enzymes which initiate CoA ligation to ibuprofen, followed by aromatic ring activation by a dioxygenase and retroaldol cleavage to unequivocally produce 4-isobutylcatechol and propionyl-CoA which then undergo further degradation. In liquid mineral salts medium, the strain eliminated more than 2 mM ibuprofen within 74 h with a generation time of 16 h. Upon inoculation into biopurification systems, it eliminated repeated doses of ibuprofen within a few days. Furthermore, in these systems the presence of RW412 avoided the accumulation of ibuprofen metabolites. In ibuprofen-spiked effluent from a municipal WWTP, ibuprofen removal by this strain was 7 times faster than by the indigenous microbiota. These results suggest that this strain can persist and remain active under environmentally relevant conditions, and may be a useful innovation to eliminate this emerging contaminant from urban wastewater treatment systems.This study was supported by the Spanish Ministerio de Economía y Competitividad and the Agencia Estatal de Investigación (AEI) (projects CTM 2013-44271-R and CTM 2017-86504-R), and by co-financing from the European Regional Development Fund (ERDF). J.D. Van Hamme is funded with a Natural Sciences and Engineering Research Council of Canada Discovery Grant (RGPIN-2019-06428). We thank Emasagra S.A. for providing effluent samples from a municipal wastewater treatment plant in Granada, Spain. Inés Aguilar-Romero was sponsored by an FPI grant

Cosmopolitan heterotrophic microeukaryotes are active bacterial grazers in experimental oil-polluted systems

We investigated the population dynamics and prevailing 18S rDNA phylotypes of microeukaryotes (<= 10 mu m) in microcosms containing seawater from either an unpolluted oligotrophic site or a chronically oil-polluted mesotrophic site of the Aegean Sea, amended with crude oil (100 p.p.m. final concentration) and crude oil plus emulsifier (10 p.p.m. final concentration). The addition of oil alone did not result in an important increase of bacteria or their predators, while the addition of oil and emulsifiers caused an important increase in bacteria followed by nanoflagellate predator response. We observed an important shift in the microeukaryotic community structure, which was characterized by the dominance of the same heterotrophic nanoflagellates in all oil-polluted treatments. Thus, the resulting 18S rDNA phylotypes were dominated (48.1-82.4%) by Paraphysomonas foraminifera in all treatments containing crude oil and crude oil plus emulsifier. The origin of the seawater, i.e. unpolluted versus chronically oil-polluted, had no effect on the dominant eukaryote, suggesting that the ubiquitous P. foraminifera is an effective opportunist in oil-polluted aquatic systems. The next dominant phylotypes were Monosiga brevicollis (<= 27.0%) and Pseudobodo tremulans (<= 23.1%). However, the addition of the emulsifier increased the dominance of P. foraminifera but decreased that of M. brevicollis and P. tremulans. Our study revealed that these dominant oil-tolerant eukaryotes, which are commonly found in the marine environments, are important grazers of bacteria and as such their dynamics should be taken into account in bioremediation practices in situ

Characterization by electrospray ionization and tandem mass spectrometry of rhamnolipids produced by two Pseudomonas aeruginosa strains isolated from Brazilian crude oil

In this work, biosurfactants produced by two Pseudomonas aeruginosa strains isolated from Brazilian crude oils were identified by proton nuclear magnetic resonance ((1)H NMR) and further characterized by mass spectrometry (MS) coupled with electrospray ionization (ESI) and tandem mass spectrometry (MS/MS) analysis in positive mode and their surface activities evaluated. Mono-rhamnolipids and di-rhamnolipids were identified for both isolates, but the most abundant were found to be mono-rhamnolipids. The similarity of rhamnolipids produced by the two strains was in good agreement with their surface activities. Both biosurfactants exhibited similar aqueous solution surface tensions, high emulsification indexes and critical micelle concentration values. The results obtained show that ESI-MS and MS/MS analysis alone provide a fast and highly specific characterization of biosurfactants produced by microbial strains.This work was supported by PARTEX OIL AND GAS. The authors acknowledge the financial support from Fundacao para a Ciencia e a Tecnologia for the Pest-C/CTM/LA0011/2011 and doctoral research grant of Jorge F.B. Pereira SFRH/BD/60228/2009