Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages λ drif d 18 and λ dfus -3

The presence of EF-Tu, RNA polymerase subunit α, and EF-G on the λ dfus -3 genome and EF-Tu, ribosomal proteins L7/L12, and RNA polymerase subunit β on the λ drif d 18 genome has been confirmed using a two-dimensional gel electrophoresis technique sensitive to changes in isoelectric point and molecular weight. In this system two EF-Tu gene products could not be resolved. Following infection of ultraviolet light-irradiated Escherichia coli with either λ dfus -3 or λ drif d 18, the EF-Tu gene, tufA , near 65 minutes on the genetic map is expressed as 3–4 copies per EF-G molecule. The EF-Tu gene, tufB , near 79 minutes on the genetic map, is expressed at about one-third of this rate. α is expressed as 1 copy per EF-G molecule, β as 0.14 per EF-G molecule and L7/L12 as 2.5 per EF-G. These figures compare well with the relative amounts found in exponentially-growing cells, in which the ratio of EF-Tu to EF-G is approximately 5. Almost 90% of the total number of proteins (calculated on a molecular weight basis) which theoretically can be encoded on the λ drif d 18 have been identified on the two-dimensional gel.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47541/1/438_2004_Article_BF00341733.pd

Mental Suffering Of Nurses Who Care For Cancer Patients [o Sofrimento Psíquico Dos Enfermeiros Que Lidam Com Pacientes Oncológicos.]

In this qualitative study, four nurses of a teaching hospital in the city of Campinas, São Paulo-Brazil, all of them working with oncological patients were interviewed in order to verify the situation that most caused mental suffering in the day by day with these patients. Data collection were performed as proposed by the ethnographic approach and the non-directive interview and the direct observation was employed. The contain analysis has been utilized to the data analysis. The results have shown that many situations of the relationship nurses-oncological patients cause mental suffering in that professional. Those situations are tied to the work of the nurses, to the kind of patient treated by those professionals and to the kind of sickness presented by the patient. That study has shown that, many times, the nurses utilize defense mechanisms against that mental suffering. This work aims to contribute to the knowledge of the subject and therefore be itself a base in which one could elaborate measures that can make these problems that occur with the nurses that work in the Oncology area lighter. With that study to contribute to the comprehension of the challenge that the cancer, sickness that make professional and population frightened, is.51227329

Nurse's Meaning Of Care For Hospitalized Children With Chronic Diseases Accompanied By A Family Member [significado Atribuído Pelo Enfermeiro Ao Cuidado Prestado A Criança Doente Crônica Hospitalizada Acompanhada De Familiar.]

This qualitative and exploratory study investigated the meaning of care for hospitalized children with chronic diseases, accompanied by a family member. Participants were six nurses, who worked in a Pediatric Ward of the teaching hospital of Campinas State University, São Paulo, Brazil. The technique used for collecting data was semi-direct interview. Thematic Content Analysis introduced the thematic category "Care for chronically ill children with focus on the disease". This care approach in the nurse's practice was shown to be predominant.28225025

Potential impacts of climate change on the productivity and soil carbon stocks of managed grasslands

International audienceRain-fed pastoral systems are tightly connected to meteorological conditions. It is, therefore, likely that climate change, including changing atmospheric CO2 concentration, temperature, precipitation and patterns of climate extremes, will greatly affect pastoral systems. However, exact impacts on the productivity and carbon dynamics of these systems are still poorly understood, particularly over longtime scales. The present study assesses the potential effects of future climatic conditions on productivity and soil organic carbon (SOC) stocks of mowed and rotationally grazed grasslands in France. We used the CenW ecosystem model to simulate carbon, water, and nitrogen cycles in response to changes in environmental drivers and management practices. We first evaluated model responses to individual changes in each key meteorological variable to get better insights into the role and importance of each individual variable. Then, we used 3 sets of meteorological variables corresponding to 3 Representative Concentration Pathways (RCP 2.6, RCP 4.5 and RCP 8.5) for long-term model runs from 1975 to 2100. Finally, we used the same three RCPs to analyze the responses of modelled grasslands to extreme climate events. We found that increasing temperature slightly increased grasslands productivities but strongly reduced SOC stocks. A reduction in precipitation led to reductions of biomass and milk production but increased SOC. Conversely, doubling CO2 concentration strongly increased biomass and milk production and marginally reduced SOC. These SOC trends were unexpected. They arose because both increasing precipitation and CO2 increased photosynthetic carbon gain, but they had an even greater effect on the proportion of biomass that could be grazed. The amount of carbon remaining on site and able to contribute to SOC formation was actually reduced under both higher precipitation and CO2. The simulations under the three RCPs indicated that grassland productivity was increased, but that required higher N fertilizer application rates and also led to substantial SOC losses. We thus conclude that, while milk productivity may continue at current rates under climate change, or even increase slightly, there could be some soil C losses over the 21st century. In addition, under the highest-emission scenario, the increasing importance of extreme climate conditions (heat waves and droughts) might render conditions at our site in some years as unsuitable for milk production. It highlights the importance of tailoring farming practices to achieve the dual goals of maintaining agricultural production while safeguarding soil C stocks