Continuous-feed nanocasting process for the synthesis of bismuth nanowire composites

We present a novel, continuous-feed nanocasting procedure for the synthesis of bismuth nanowire structures embedded in the pores of a mesoporous silica template. The immobilization of a bismuth salt inside the silica template from a diluted metal salt solution yields a sufficiently high loading to obtain electrically conducting bulk nanowire composite samples after reduction and sintering the nanocomposite powders. Electrical resistivity measurements of sintered bismuth nanowires embedded in the silica template reveal size-quantization effects

Identification and expression analysis of genes associated with bovine blastocyst formation

<p>Abstract</p> <p>Background</p> <p>Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation of the embryonic genome, compaction and blastocyst formation.</p> <p>First lineage differentiation starts at the blastocyst stage with the formation of the trophectoderm and the inner cell mass. The main objective of this study was the detection, identification and expression analysis of genes associated with blastocyst formation in order to help us better understand this process. This information could lead to improvements of <it>in vitro </it>embryo production procedures.</p> <p>Results</p> <p>A subtractive cDNA library was constructed enriched for transcripts preferentially expressed at the blastocyst stage compared to the 2-cell and 8-cell stage. Sequence information was obtained for 65 randomly selected clones. The RNA expression levels of 12 candidate genes were determined throughout 3 stages of preimplantation embryo development (2-cell, 8-cell and blastocyst) and compared with the RNA expression levels of <it>in vivo </it>"golden standard" embryos using real-time PCR. The RNA expression profiles of 9 (75%) transcripts (<it>KRT18</it>, <it>FN1</it>, <it>MYL6</it>, <it>ATP1B3</it>, <it>FTH1</it>, <it>HINT1</it>, <it>SLC25A5</it>, <it>ATP6V0B</it>, <it>RPL10</it>) were in agreement with the subtractive cDNA cloning approach, whereas for the remaining 3 (25%) (<it>ACTN1</it>, <it>COPE</it>, <it>EEF1A1</it>) the RNA expression level was equal or even higher at the earlier developmental stages compared to the blastocyst stage. Moreover, significant differences in RNA expression levels were observed between <it>in vitro </it>and <it>in vivo </it>produced embryos. By immunofluorescent labelling, the protein expression of KRT18, FN1 and MYL6 was determined throughout bovine preimplantation embryo development and showed the same pattern as the RNA expression analyses.</p> <p>Conclusion</p> <p>By subtractive cDNA cloning, candidate genes involved in blastocyst formation were identified. For several candidate genes, important differences in gene expression were observed between <it>in vivo </it>and <it>in vitro </it>produced embryos, reflecting the influence of the <it>in vitro </it>culture system on the embryonic gene expression. Both RNA and protein expression analysis demonstrated that <it>KRT18</it>, <it>FN1 </it>and <it>MYL6 </it>are differentially expressed during preimplantation embryo development and those genes can be considered as markers for bovine blastocyst formation.</p

Load-based generic polca: performance assessement using simulation

POLCA (i.e. Paired-cell Overlapping Loops of Cards with Authorization) is a card-based decision support system for production control, developed to support the adoption of Quick Response Manufacturing. Two variants of POLCA have been proposed in the literature to improve POLCA performance: Load Based POLCA and Generic POLCA. In this paper, we combine these two variants into a single production control system and analyse its performance for different backlog-sequencing rules. The results of a simulation study carried out for a make-to-order flow shop, support the strategy of combining these two POLCA variants and show that capacity-slack backlog sequencing based on corrected aggregate load have the potential for improving performance.info:eu-repo/semantics/publishedVersio

Reduction of Mycobacterium avium ssp. paratuberculosis in colostrum : development and validation of 2 methods, one based on curdling and one based on centrifugation

The aim of this study was to develop and validate 2 protocols (for use on-farm and at a central location) for the reduction of Mycobacterium. avium ssp. paratuberculosis (MAP) in colostrum while preserving beneficial immunoglobulins (IgG). The on-farm protocol was based on curdling of the colostrum, where the IgG remain in the whey and the MAP bacteria are trapped in the curd. First, the colostrum was diluted with water (2 volumes colostrum to 1 volume water) and 2% rennet was added. After incubation (1 11 at 32 degrees C), the curd was cut and incubated again, after which whey and curd were separated using a cheesecloth. The curd was removed and milk powder was added to the whey. Approximately 1 log reduction in MAP counts was achieved. A reduction in total proteins and IgG was observed due to initial dilution of the colostrum. After curd formation, more than 95% of the immunoglobulins remained in the whey fraction. The semi-industrial protocol was based on centrifugation, which causes MAP to precipitate, while the IgG remain in the supernatant. This protocol was first developed in the laboratory. The colostrum was diluted with skimmed colostrum (2 volumes colostrum to 1 volume skimmed colostrum), then skimmed and centrifuged (at 15,600 x g for 30 min at room temperature). We observed on average 1.5 log reduction in the MAP counts and a limited reduction in proteins and IgG in the supernatant. To obtain a semi-industrial protocol, dairy pilot appliances were evaluated and the following changes were applied to the protocol: after 2:1 dilution as above, the colostrum was skimmed and subsequently clarified, after which the cream was heat treated and added to the supernatant. To investigate the effect of the colostrum treatment on the nutritional value and palatability of the colostrum and the IgG transfer, an animal experiment was conducted with 24 calves. Six received the dam's colostrum, 6 were given untreated purchased colostrum (control), and 2 groups of 6 calves received colostrum treated according to both of the above-mentioned methods. No significant differences were found between the test groups and the dam's colostrum group in terms of animal health, IgG uptake in the blood serum, milk; or forage uptake. Two protocols to reduce MAP in colostrum (for use on-farm or at a central location) were developed. Both methods preserve the vital IgG

Water vapour line assignments in the 9250-26 000 cm (-1) frequency range

Line parameters for water vapour in natural abundance have recently been determined for the 9250-13 000 cm(-1) region [M.-F. Wrienne, A. Jenouvrier, C. Hermans, A.C. Vandaele, M. Carleer, C. Clerbaux, P.-F. Coheur, R. Colin, S. Fally, M. Bach, J. Quant. Spectrosc. Radiat. Transfer 82 (2003) 99] and the 13 000-26 000 cm(-1) region [P.-F. Coheur, S. Fally, M. Carleer, C. Clerbaux, R. Colin, A. Jenouvrier, M.-F. Wrienne, C. Hermans, A.C. Vandaele, J. Quant. Spectrosc. Radial. Transfer 74 (2002) 493] using a high-resolution Fourier-transform spectrometer with a long-path absorption cell. These spectra are analysed using several techniques including variational line lists and assignments made. In total, over 15 000 lines were assigned to transitions involving more than 150 exited vibrational states of (H2O)-O-16. Twelve new vibrational band origins are determined and estimates for a further 16 are presented. (c) 2005 Elsevier Inc. All rights reserved

Lever Insertion as a Salient Stimulus Promoting Insensitivity to Outcome Devaluation

Flexible and efficient decision-making in complex environments can be achieved through constant interactions between the goal-directed and habitual systems. While goal-directed behavior is considered dependent upon Response-Outcome (R-O) associations, habits instead rely on Stimulus-Response (S-R) associations. However, the stimuli that support the S-R association underlying habitual responding in typical instrumental procedures are poorly defined. To resolve this issue, we designed a discrete-trials procedure, in which rats must wait for lever insertion and complete a sequence of five lever presses to obtain a reward (20% sucrose or grain-based pellets). Lever insertion thus constituted an audio-visual stimulus signaling the opportunity for reward. Using sensory-specific satiety-induced devaluation, we found that rats trained with grain-based pellets remained sensitive to outcome devaluation over the course of training with this procedure whereas rats trained with a solution of 20% sucrose rapidly developed habit, and that insensitivity to outcome devaluation in rats trained with sucrose did not result from a bias in general satiety. Importantly, although rats trained with pellets were sensitive to satiety-induced devaluation, their performance was not affected by degradation of instrumental contingency and devaluation by conditioned taste aversion (CTA), suggesting that these rats may also have developed habitual responding. To test whether the discrete-trials procedure biases subjects towards habitual responding, we compared discrete-trials to free-running instrumental responding, and found that rats trained with sucrose in a fixed-ratio 5 (FR5) procedure with continuous presentation of the lever were goal-directed. Together, these results demonstrate that discrete presentations of a stimulus predictive of reward availability promoted the formation of S-R habit in rats trained with liquid sucrose. Further research is necessary to explain inconsistencies in sensitivity to outcome devaluation when rats are trained with grain-based pellets

Participation of women scientists in ESA solar system missions: A historical trend

We analyzed the participation of women scientists in 10 ESA (European Space Agency) Solar System missions over a period of 38 years. Being part of a spacecraft mission science team can be considered a proxy to measure the "success"in the field. Participation of women in PI (Principal Investigators) teams varied between 4% and 25 %, with several missions with no women as PI. The percentage of female scientists as Co-I (Co-Investigators) is always less than 16 %. This number is lower than the percentage of women in the International Astronomical Union from all ESA's Member State (24 %), which can give us an indication of the percentage of women in the field. We encountered many difficulties to gather the data for this study. The list of team members were not always easily accessible. An additional difficulty was to determine the percentage of female scientists in planetary science in Europe. We would like to encourage the planetary community as a whole, as well as international organizations, universities and societies to continuously gather statistics over many years. Detailed statistics are only the first step to closely monitor the development of achievement gaps and initiate measures to tackle potential causes of inequity, leading to gender inequalities in STEM careers

Cell arrest and cell death in mammalian preimplantation development

The causes, modes, biological role and prospective significance of cell death in preimplantation development in humans and other mammals are still poorly understood. Early bovine embryos represent a very attractive experimental model for the investigation of this fundamental and important issue. To obtain reference data on the temporal and spatial occurrence of cell death in early bovine embryogenesis, three-dimensionally preserved embryos of different ages and stages of development up to hatched blastocysts were examined in toto by confocal laser scanning microscopy. In parallel, transcript abundance profiles for selected apoptosis-related genes were analyzed by real-time reverse transcriptase-polymerase chain reaction. Our study documents that in vitro as well as in vivo, the first four cleavage cycles are prone to a high failure rate including different types of permanent cell cycle arrest and subsequent non-apoptotic blastomere death. In vitro produced and in vivo derived blastocysts showed a significant incidence of cell death in the inner cell mass (ICM), but only in part with morphological features of apoptosis. Importantly, transcripts for CASP3, CASP9, CASP8 and FAS/FASLG were not detectable or found at very low abundances. In vitro and in vivo, errors and failures of the first and the next three cleavage divisions frequently cause immediate embryo death or lead to aberrant subsequent development, and are the main source of developmental heterogeneity. A substantial occurrence of cell death in the ICM even in fast developing blastocysts strongly suggests a regular developmentally controlled elimination of cells, while the nature and mechanisms of ICM cell death are unclear. Morphological findings as well as transcript levels measured for important apoptosis-related genes are in conflict with the view that classical caspase-mediated apoptosis is the major cause of cell death in early bovine development