The George C. Davis Site, Cherokee County, Texas: Spring 1980 Archeological Investigations

The results of archeological investigations in portions of the George C. Davis Site are presented in this report prepared by Ross Fields and J. Peter Thurmond . Stringent contract requirements and the provisions of Texas Antiquities Permit No. 237 dictate that this report be brief and descriptive in nature. Requirements such as inclusion of a detailed site investigation history and environmental data were excluded and extensive excavations were stressed in preference to detailed analyses and comparisons. Field investigations were limited by contract to 20 working days and the analysis/write-up to 10 working days. The governing research design was prepared by the Texas Antiquities Committee staff to reflect agreements between the Committee and the Texas Forest Service. Sighificant findings include the general delineation of extensive, and in places intensive, Archaic and Late Prehistoric occupations. Of primary importance is the lack of an identifiable Alto Focus occupation within the northern portion of the Davis Site. This suggests that the small left bank tributary to Bowles Creek existed during Alto Focus times and served as a natural boundary for the village area during that period of site usage. There are hints that significant variations in the selection and use of lithic resources through time are identifiable. It is indeed ! unfortunate that time limitations precluded pursuit of this line of investigation. Ross and Pete have successfully accomplished a most challenging task under less than ideal conditions. They and their field crew are commended for their outstanding work at the Davis Site

Ecology and Mary: An Ecological Theology of Mary as the New Eve in Response to the Church’s Challenge for a Faith-Based Education in Ecological Responsibility

The Church’s interpretation of the current ecological crisis as a moral crisis is the catalyst for this essay, which proposes a newly constructed faith-based model for ecological dialogue and education. The exploration and reinterpretation of the traditional Church doctrine of the Virgin Mary as the new Eve provides a theme from which an ecological theology of Mary is constructed. Papal and Episcopal statements that call for a moral concern and response to the growing urgency of the ecological crisis are discussed in order to promote awareness of the involvement of the Church’s leadership in the issue. Analyses and interpretations by scholars in the second century Church on the doctrine of Mary as the new Eve are presented and reinterpreted to create a viable model with the potential to nurture ecological awareness and responsibility in the contemporary Church. The construction of an ecological Marian theology is approached through review and analysis of the 1974 visionary pastoral letter of Pope Paul VI, Marialis Cultus (To Honor Mary), the writings of Catholic feminist theologian and Sister of St. Joseph Elizabeth Johnson, Catholic Ecuadorian-American theologian Jeanette Rodriguez, and those of other major feminist, womanist, and liberationist theologians

Was the Cypress Cluster one of the (Many) Victims of the 1539 - 1543 De Soto Expedition?

In my master\u27s thesis on the archeology of the Cypress creek basin (Thurmond 1981) and a subsequent article in the Bulletin of the Texas Archeological Society, I proposed the identification of a third late prehistoric-protohistoric confederacy for the Caddoan area of northeast Texas, in addition to those of the Hasinai and Kadohadacho. I named the archeological manifestation of this hypothesized sociopolitical entity the Cypress cluster, following a model of late Caddoan sociopolitical organization formulated by Dee Ann Story. The Cypress cluster is centered geographically on the upper Cypress Creek, White Oak Bayou and Lake Fork Creek basins. Two sequential temporal units are defined within the Cypress cluster, the Whelan and Titus phases, perhaps dating to the 15th and 16th centuries, respectively. Components of the Titus phase are sufficiently well-documented to permit the identification of four distinct spatial subgroups within the Cypress cluster on the basis of their associated ceramic and lithic assemblages. These spatial subgroups are termed subclusters (the Three Basins, Tankersley Creek, swauano Creek and Big Cypress Creek subclusters), and probably represent the archeological remains of four affiliated tribal groups

DNA Spools under Tension

DNA-spools, structures in which DNA is wrapped and helically coiled onto itself or onto a protein core are ubiquitous in nature. We develop a general theory describing the non-equilibrium behavior of DNA-spools under linear tension. Two puzzling and seemingly unrelated recent experimental findings, the sudden quantized unwrapping of nucleosomes and that of DNA toroidal condensates under tension are theoretically explained and shown to be of the same origin. The study provides new insights into nucleosome and chromatin fiber stability and dynamics

Highly Coordinated Gene Regulation in Mouse Skeletal Muscle Regeneration

Mammalian skeletal muscles are capable of regeneration after injury. Quiescent satellite cells are activated to reenter the cell cycle and to differentiate for repair, recapitulating features of myogenesis during embryonic development. To understand better the molecular mechanism involved in this process in vivo, we employed high density cDNA microarrays for gene expression profiling in mouse tibialis anterior muscles after a cardiotoxin injection. Among 16,267 gene elements surveyed, 3,532 elements showed at least a 2.5-fold change at one or more time points during a 14-day time course. Hierarchical cluster analysis and semiquantitative reverse transcription-PCR showed induction of genes important for cell cycle control and DNA replication during the early phase of muscle regeneration. Subsequently, genes for myogenic regulatory factors, a group of imprinted genes and genes with functions to inhibit cell cycle progression and promote myogenic differentiation, were induced when myogenic stem cells started to differentiate. Induction of a majority of these genes, including E2f1 and E2f2, was abolished in muscles lacking satellite cell activity after gamma radiation. Regeneration was severely compromised in E2f1 null mice but not affected in E2f2 null mice. This study identifies novel genes potentially important for muscle regeneration and reveals highly coordinated myogenic cell proliferation and differentiation programs in adult skeletal muscle regeneration in vivo

The Histamine H4 Receptor Mediates Inflammation and Pruritus in Th2-Dependent Dermal Inflammation

The role of histamine H4 receptor (H4R) was investigated in a T-helper type 2 (Th2)-cell-mediated mouse skin inflammation model that mimics several of the features of atopic dermatitis. Treatment with two specific H4R antagonists before challenge with FITC led to a significant reduction in ear edema, inflammation, mast cell, and eosinophil infiltration. This was accompanied by a reduction in the levels of several cytokines and chemokines in the ear tissue. Upon ex vivo antigen stimulation of lymph nodes, H4R antagonism reduced lymphocyte proliferation and IL-4, IL-5, and IL-17 levels. One explanation for this finding is that lymph nodes from animals dosed with the H4R antagonist, JNJ 7777120, contained a lower number of FITC-positive dendritic cells. The effect of H4R antagonism on dendritic cell migration in vivo may be an indirect result of the reduction in tissue cytokines and chemokines or a direct effect on chemotaxis. In addition to anti-inflammatory effects, JNJ 7777120 also significantly inhibited the pruritus shown in the model. Therefore, the dual effects of H4R antagonists on pruritus and Th2-cell-mediated inflammation point to their therapeutic potential for the treatment of Th2-mediated skin disorders, including atopic dermatitis

Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c <i>In Vitro</i>

Background: Translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the plasma membrane is responsible for the increased rate of glucose transport into fat and muscle cells in response to insulin. This represents a specialised form of regulated membrane trafficking. Intracellular membrane traffic is subject to multiple levels of regulation by conserved families of proteins in all eukaryotic cells. Notably, all intracellular fusion events require SNARE proteins and Sec1p/Munc18 family members. Fusion of GLUT4-containing vesicles with the plasma membrane of insulin-sensitive cells involves the SM protein Munc18c, and is regulated by the formation of syntaxin 4/SNAP23/VAMP2 SNARE complexes. Methodology/Principal Findings Here we have used biochemical approaches to characterise the interaction(s) of Munc18c with its cognate SNARE proteins and to examine the role of Munc18c in regulating liposome fusion catalysed by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. We demonstrate that Munc18c makes contacts with both t- and v-SNARE proteins of this complex, and directly inhibits bilayer fusion mediated by the syntaxin 4/SNAP23/VAMP2 SNARE complex. Conclusion/Significance Our reductionist approach has enabled us to ascertain a direct inhibitory role for Munc18c in regulating membrane fusion mediated by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. It is important to note that two different SM proteins have recently been shown to stimulate liposome fusion mediated by their cognate SNARE complexes. Given the structural similarities between SM proteins, it seems unlikely that different members of this family perform opposing regulatory functions. Hence, our findings indicate that Munc18c requires a further level of regulation in order to stimulate SNARE-mediated membrane fusion

FlyBase 101 – the basics of navigating FlyBase

FlyBase (http://flybase.org) is the leading database and web portal for genetic and genomic information on the fruit fly Drosophila melanogaster and related fly species. Whether you use the fruit fly as an experimental system or want to apply Drosophila biological knowledge to another field of study, FlyBase can help you successfully navigate the wealth of available Drosophila data. Here, we review the FlyBase web site with novice and less-experienced users of FlyBase in mind and point out recent developments stemming from the availability of genome-wide data from the modENCODE project. The first section of this paper explains the organization of the web site and describes the report pages available on FlyBase, focusing on the most popular, the Gene Report. The next section introduces some of the search tools available on FlyBase, in particular, our heavily used and recently redesigned search tool QuickSearch, found on the FlyBase homepage. The final section concerns genomic data, including recent modENCODE (http://www.modencode.org) data, available through our Genome Browser, GBrowse

Inflammatory stress of pancreatic beta cells drives release of extracellular heat-shock protein 90α

A major obstacle in predicting and preventing the development of autoimmune type 1 diabetes (T1D) in at-risk individuals is the lack of well-established early biomarkers indicative of ongoing beta cell stress during the pre-clinical phase of disease. Recently, serum levels of the α cytoplasmic isoform of heat-shock protein 90 (hsp90) were shown to be elevated in individuals with new-onset T1D. We therefore hypothesized that hsp90α could be released from beta cells in response to cellular stress and inflammation associated with the earliest stages of T1D. Here, human beta cell lines and cadaveric islets released hsp90α in response to stress induced by treatment with a combination of pro-inflammatory cytokines including interleukin-1β, tumour necrosis factor-α and interferon-γ. Mechanistically, hsp90α release was found to be driven by cytokine-induced endoplasmic reticulum stress mediated by c-Jun N-terminal kinase (JNK), a pathway that can eventually lead to beta cell apoptosis. Cytokine-induced beta cell hsp90α release and JNK activation were significantly reduced by pre-treating cells with the endoplasmic reticulum stress-mitigating chemical chaperone tauroursodeoxycholic acid. The hsp90α release by cells may therefore be a sensitive indicator of stress during inflammation and a useful tool in assessing therapeutic mitigation of cytokine-induced cell damage linked to autoimmunity

A Guide For Irrigating Fruits and Vegetables in East Texas.

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