Quantitative Topographical Characterization of Thermally Sprayed Coatings by Optical Microscopy

Topography measurements and roughness calculations for different rough surfaces (Rugotest surface comparator and thermally sprayed coatings) are presented. The surfaces are measured with a novel quantitative topography measurement technique based on optical stereomicroscopy and a comparison is made with established scanning stylus and optical profilometers. The results show that for most cases the different methods yield similar results. Stereomicroscopy is therefore a valuable method for topographical investigations in both quality control and research. On the other hand, the method based on optical microscopy demands a careful optimization of the experimental settings like the magnification and the illumination to achieve satisfactory result

NASA's NPOESS Preparatory Project Science Data Segment: A Framework for Measurement-based Earth Science Data Systems

The NPOESS Preparatory Project (NPP) Science Data Segment (SDS) provides a framework for the future of NASA s distributed Earth science data systems. The NPP SDS performs research and data product assessment while using a fully distributed architecture. The components of this architecture are organized around key environmental data disciplines: land, ocean, ozone, atmospheric sounding, and atmospheric composition. The SDS thus establishes a set of concepts and a working prototypes. This paper describes the framework used by the NPP Project as it enabled Measurement-Based Earth Science Data Systems for the assessment of NPP products

A comparison of microtensile and microcompression methods for studying plastic properties of nanocrystalline electrodeposited nickel at different length scales

A comparison of microcompression and microtensile methods to study mechanical properties of electrodeposited nanocrystalline (nc) nickel has been performed. Microtensile tests that probe a volume of more than 2 × 106 μm3 show reasonable agreement with results from microcompression tests that probe much smaller volumes down to a few μm3. Differences between the two uniaxial techniques are discussed in terms of measurements errors, probed volume and surface effects, strain rate, and influence of stress state. Uniaxial solicitation in compression mode revealed several advantages for studying stress-strain propertie

A comparison of microtensile and microcompression methods for studying plastic properties of nanocrystalline electrodeposited nickel at different length scales

A comparison of microcompression and microtensile methods to study mechanical properties of electrodeposited nanocrystalline (nc) nickel has been performed. Microtensile tests that probe a volume of more than 2 × 106 μm3 show reasonable agreement with results from microcompression tests that probe much smaller volumes down to a few μm3. Differences between the two uniaxial techniques are discussed in terms of measurements errors, probed volume and surface effects, strain rate, and influence of stress state. Uniaxial solicitation in compression mode revealed several advantages for studying stress–strain properties

The EF-hand Ca²⁺-binding protein super-family: A genome-wide analysis of gene expression patterns in the adult mouse brain

In mice, 249 putative members of the superfamily of EF-hand domain Ca²⁺-binding proteins, manifesting great diversity in structure, cellular localization and functions have been identified. Three members in particular, namely, calbindin-D28K, calretinin and parvalbumin, are widely used as markers for specific neuronal subpopulations in different regions of the brain. The aim of the present study was to compile a comprehensive atlas of the gene-expression profiles of the entire EF-hand gene superfamily in the murine brain. This was achieved by a meticulous examination of the in-situ hybridization images in the Allen Brain Atlas database. Topographically, our analysis focused on the olfactory bulb, cerebral cortex (barrel cortex in the primary somatosensory area), basal ganglia, hippocampus, amygdala, thalamus, hypothalamus, cerebellum, midbrain, pons and medulla, and on clearly identifiable sub-structures within each of these areas. The expression profiles of four family-members, namely hippocalcin-like 4, neurocalcin-δ, plastin 3 and tescalcin, that have not been hitherto reported, at either the mRNA (in-situ-hybridization) or the protein (immunohistochemical) levels, are now presented for the first time. The fruit of our analysis is a document in which the gene-expression profiles of all members of the EF-hand family genes are compared, and in which future possible neuronal markers for specific cells/brain areas are identified. The assembled information could afford functional clues to investigators, conducive to further experimental pursuit

Comparison of nanoindentation results obtained with Berkovich and cube-corner indenters

There is increasing interest in using sharp cube corner indenters in nanoindentation experiments to study plastic properties. In combination with finite element methods it is for instance possible to extract stress-strain curves from load-displacement curves measured with differently shaped pyramidal indenters. Another example is the fracture toughness of coatings, which can be studied using cracks produced during indentation with cube corner tips. We have carried out indentation experiments with Berkovich and cube corner indenters on eight different materials with different mechanical properties. To gain information about the formation of pile-up and cracks, indentation experiments with cube corner indenter were performed inside a Scanning Electron Microscope (SEM) using a custom-built SEMMicroindenter. The results show that reliable hardness and modulus values can be measured using cube corner indenters. However, the fit range of the unloading curve has a much bigger influence on the results for the cube corner than for the Berkovich tip. The unloading curves of a cube corner measurement should therefore be carefully inspected to determine the region of smooth curvature and the unloading fit range chosen warily. Comparison of the modulus results shows that there is no significant difference between cube corner and Berkovich measurements. Also for hardness, no fundamental difference is observed for most of the investigated materials. Exceptions are materials like silicon nitride, cemented carbide or glassy carbon, where a clear difference to the hardness reference value has been observed although the modulus difference is not pronounced

Loss of CCDC6 Affects Cell Cycle through Impaired Intra-S-Phase Checkpoint Control

In most cancers harboring Ccdc6 gene rearrangements, like papillary thyroid tumors or myeloproliferative disorders, the product of the normal allele is supposed to be functionally impaired or absent. To address the consequence of the loss of CCDC6 expression, we applied lentiviral shRNA in several cell lines. Loss of CCDC6 resulted in increased cell death with clear shortening of the S phase transition of the cell cycle. Upon exposure to etoposide, the cells lacking CCDC6 did not achieve S-phase accumulation. In the absence of CCDC6 and in the presence of genotoxic stress, like etoposide treatment or UV irradiation, increased accumulation of DNA damage was observed, as indicated by a significant increase of pH2Ax Ser139. 14-3-3σ, a major cell cycle regulator, was down-regulated in CCDC6 lacking cells, regardless of genotoxic stress. Interestingly, in the absence of CCDC6, the well-known genotoxic stress-induced cytoplasmic sequestration of the S-phase checkpoint CDC25C phosphatase did not occur. These observations suggest that CCDC6 plays a key role in cell cycle control, maintenance of genomic stability and cell survival and provide a rational of how disruption of CCDC6 normal function contributes to malignancy

Comparison of TRMM 2A25 Products Version 6 and Version 7 with NOAA/NSSL Ground Radar-Based National Mosaic QPE

Characterization of the error associated to satellite rainfall estimates is a necessary component of deterministic and probabilistic frameworks involving spaceborne passive and active microwave measurements for applications ranging from water budget studies to forecasting natural hazards related to extreme rainfall events. We focus here on the error structure of Tropical Rainfall Measurement Mission (TRMM) Precipitation Radar (PR) quantitative precipitation estimation (QPE) at ground. The problem was addressed in a previous paper by comparison of 2A25 version 6 (V6) product with reference values derived from NOAA/NSSL's ground radar-based National Mosaic and QPE system (NMQ/Q2). The primary contribution of this study is to compare the new 2A25 version 7 (V7) products that were recently released as a replacement of V6. This new version is considered superior over land areas. Several aspects of the two versions are compared and quantified including rainfall rate distributions, systematic biases, and random errors. All analyses indicate V7 is an improvement over V6

The Coherence Field in the Field Perturbation Theory of Superconductivity

We re-examine the Nambu-Gorkov perturbation theory of superconductivity on the basis of the Bogoliubov-Valatin quasi-particles. We show that two different fields (and two additional analogous fields) may be constructed, and that the Nambu field is only one of them. For the other field- the coherence field- the interaction is given by means of two interaction vertices that are based on the Pauli matrices tau1 and tau3. Consequently, the Hartree integral for the off-diagonal pairing self-energy may be finite, and in some cases large. We interpret the results in terms of conventional superconductivity, and also discuss briefly possible implications to HTSC