Optical-trapping micromanipulation using 780-nm diode lasers

We have designed and implemented an optical-trapping configuration that uses near-infrared laser diodes. The highly divergent output beam of the diode laser was collimated by using only one aspheric compact disc lens. The resulting output beams are astigmatic and elliptic and have a flat, non-Gaussian intensity profile. Calculations and measurements were performed to investigate the influence of this profile on the trapping forces. The results show that use of a laser diode, collimated with a compact disc lens, provides a near-infrared light source that can be used for optical trapping. The light source is compact and relatively cheap and can be easily incorporated into an existing microscope

Modeling Decentralized Organizational Change in Honeybee Societies

Multi-agent organizations in dynamic environments, need to have the ability to adapt to environmental changes to ensure a continuation of proper functioning. Such adaptations can be made through a centralized decision process or come from the individuals within the organization. In the domain of social insects, such as honeybees and wasps, organizations are known to adapt in a decentralized fashion to environmental changes. An organizational model for decentralized organizational change is presented that can aid in analyzing and designing such organizations. The model is specified by dynamic properties at different aggregation levels. At the lowest level such properties characterize the behavior of individual roles, which can be related to higher level properties that express important elements such as survival of an organization. A honeybee colony is used as a case study

Application of Raman Microspectroscopic and Raman imaging techniques for cell biological studies

Raman spectroscopy is being used to study biological molecules for some three decades now. Thanks to continuing advances in instrumentation more and more applications have become feasible in which molecules are studied in situ, and this has enabled Raman spectroscopy to enter the realms of biomedicine and cell biology [1-5].\ud Here we will describe some of the recent work carried out in our laboratory, concerning studies of human white blood cells and further instrumentational developments

Analysis of Meeting Protocols by Formalisation, Simulation and Verification

Organizations depend on regular meetings to carry out their everyday tasks. When carried out successfully, meetings offer a common medium for participants to exchange ideas and make decisions. However, many meetings suffer from unfocused discussions or irrelevant dialogues. To study meetings in detail, we first formalize general properties of meetings and a generic meeting protocol to specify how roles in a meeting should interact to realize these properties. This generic protocol is used as a starting point to study real-life meetings. Next, an example meeting is simulated using the generic meeting protocol. The general properties are formally verified in the simulation trace. Next, these properties are also verified formally against empirical data of a real meeting in the same context. A comparison of the two traces reveals that a real meeting is more robust since when exceptions happen and the rules of the protocol are violated, these exceptions are handled effectively. Given this observation, a more refined protocol is specified that includes exception-handling strategies. Based on this refined protocol a meeting is simulated that closely resembles the real meeting. This protocol is then validated against another set of data from another real meeting. By iteratively adding exception handling rules, the protocol is enhanced to handle a variety of situations successfully. © Springer Science+Business Media, LLC 2006

Personality traits, depression and itch in patients with atopic dermatitis in an experimental setting : A regression analysis

It is known that itch is associated with psychological variables, but it is not known whether personality characteristics, depression or anxiety are predictors of experimentally induced itch in patients with atopic dermatitis (AD). In this study itch was induced in 27 patients with AD and 28 healthy controls by the presentation of an experimental video on crawling insects and skin diseases. Itch intensity was measured by self-ratings and by observing the number of scratch movements. Itch increase was determined by subtracting itch intensity induced by the experimental video from itch intensity induced by a control video. Psychological variables were assessed using validated questionnaires. In patients with AD, depression was a significant predictor of self-rated induced itch (corrected R2 = 0.175); while agreeableness and public self-consciousness were significant predictors of induced scratching (corrected R2 = 0.534). In healthy controls no associations were found. These results imply that a special group of patients with AD might benefit from certain psychological interventions