Oregon Wine Board Meeting Minutes September 11, 2012

These meeting minutes list individuals in attendance and missing at the September 11, 2012 Oregon Wine Board (OWB) meeting, held via conference call. Dewey Weddington provided a marketing update focused on planning for Oregon Wine Month and the Oregon Wine Industry Symposium. The meeting also included discussion of the 2011-2012 year-end financial review and a presentation of the budget for the following year. The meeting lasted 2 hours 6 minutes, and the Board went into Executive Session after the meeting was adjourned

Kinematical structure of the circumstellar environments of galactic B[e]-type stars

High resolution line profiles are presented for selected forbidden and permitted emission lines of a sample of galactic B[e]-type stars. The spectral resolution corresponds to 5-7 km/s with the exception of some line profiles which were observed with a resolution of 9-13 km/s. All Ha profiles are characterized by a narrow split or single emission component with a width of about 150-250 km/s (FWHM) and broad wings with a full width of ~1000-2000 km/s. The Ha profiles can be classified into three groups: double-peaked profiles representing the majority, single-peaked emission-line profiles, and normal P Cygni-type profiles. The forbidden lines exhibit in most cases double-peaked profiles. The split forbidden line profiles have peak separations of as little as 10 km/s. The ratio of violet to red emission peak intensities, V/R, is predominantly smaller or equal to 1. Theoretical profiles were calculated for the optically thin case. A latitude-dependent stellar wind with a radial expansion and a velocity decreasing from the pole to the equator was adopted. In addition an equatorial dust ring with various optical depths was assumed. This model can explain split lines and line asymmetries observed in some stars. Moreover, the V/R ratios can be understood in terms of this model. The comparison of the observed line profiles with the models thus confirms the assumption of disk-like line-formation regions as commonly adopted for B[e]-type stars.Comment: Astronomy & Astrophysics, in pres

SwSt 1: an O-rich planetary nebula around a C-rich central star

The hydrogen-deficient carbon-rich [WCL] type central star HD167362 and its oxygen-rich planetary nebula (PN) SwSt~1 are investigated. The nebular chemistry might indicate a recent origin for the carbon-rich stellar spectrum. Its stellar and nebular properties might therefore provide further understanding of the origin of the [WCL] central star class. The UV-IR stellar spectra are modelled with state of the codes and show ~40kK central star with a wind and a C/O~3, indicative of efficient third dredge-up. The synthetic stellar flux distribution is used to model the high density, compact PN, which has a solar C/O ratio, is still enshrouded by 1200K and 230K dust shells and, reported here for the first time, in molecular hydrogen. Although it appears that the change in C/O ratio has been recent, the published spectroscopy since 1895 has been re-examined and no clear spectral change is seen. If an event occurred that has turned it into a hydrogen-deficient central star, it did not happen in the last 100 years.Comment: 31 pages, 19 figures (some are gif files), MNRAS in pres

Reclassification of <i>Vibrio hollisae</i> as <i>Grimontia hollisae</i> gen. nov., comb. nov.

The taxonomic positions of three representative strains of Vibrio hollisae (LMG 17719T, LMG 21416 and LMG 21538) were investigated by means of 16S rDNA sequences and phenotypic data. V. hollisae strains (GenBank/EMBL accession nos AJ514909–AJ514911) shared 99·5 % 16S rDNA sequence similarity, but had only 94·6 % similarity to their closest phylogenetic neighbour, Enterovibrio norvegicus. 16S rDNA sequence similarity of V. hollisae and Vibrio cholerae was only 91 %. These results suggest that V. hollisae should be placed into a novel genus, for which the name Grimontia gen. nov. is proposed

Incidence and identification of mesophilic <i>Aeromonas</i> spp. from retail foods

Sixty-eight food samples were examined for the presence of mesophilic Aeromonas species both qualitatively and quantitatively. Aeromonads were isolated from 26% of the vegetable samples, 70% of the meat and poultry samples and 72% of the fish and shrimps. Numbers of motile aeromonads present in the food samples varied from 2 cfu g-1 to >105 cfu g-1. GLC analysis of FAMEs was used to identify a selection of presumptive Aeromonas colonies to fenospecies or genomic species level. Aeromonas strains belonging to the Aer. caviae complex, which also includes the potentially pathogenic genospecies HG4, were mostly isolated from vegetables but were also found in meat, poultry and fish. In addition, three strains of the virulent taxon Aer. veronii biovar sobria HG8 were isolated from poultry and minced meat. All members of the Aer. hydrophila complex, predominant in the fish, meat and poultry samples, were classified in the non-virulent taxon HG3. Although the significance of Aeromonas in foods remains undefined, the isolation of Aeromonas HG4 and HG8 strains from a variety of retail foods may indicate that these products can act as possible vehicles for the dessimination of food-borne Aeromonas gastroenteritis

<i>Vibrio rotiferianus</i> sp. nov., isolated from cultures of the rotifer <i>Brachionus plicatilis</i>

Five Gram-negative bacterial strains, oxidase-positive, motile by means of more than one polar flagella, facultative anaerobe, arginine dihydrolase-negative, lysine- and ornithine decarboxylase-positive, sensitive to the vibriostatic agent O/129, were isolated from a flow-through rotifer culture system in Gent, Belgium, and previously characterized by fluorescent amplified fragment length polymorphism. Comparison of the 16S rDNA sequence of strain LMG 21460T indicated close relationships (~99 % similarity) to Vibrio campbellii, Vibrio harveyi, Vibrio alginolyticus and Vibrio parahaemolyticus. However, DNA hybridization experiments revealed similarity values below 70 % with its closest species V. campbellii and V. harveyi. Additionally, the analysed strains differ from related Vibrio species by the utilization of melibiose and production of acid from L-arabinose and amygdalin. Among the strains analysed, differences were observed in some phenotypic characters, particularly susceptibility to ampicillin, polymyxin B and amikacin, and urease activity. The major fatty acids identified were 16:0, 18:1 ?7c, 14:0, 12:0 3-OH and 18:0. Vibrio rotiferianus sp. nov. is proposed, with type strain LMG 21460T (=CAIM 577T); it has a DNA G+C content of 44·5 ± 0·01 mol%

<i>Alteromonas stellipolaris</i> sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus <i>Alteromonas</i>

Seven novel, cold-adapted, strictly aerobic, facultatively oligotrophic strains, isolated from Antarctic sea water, were investigated by using a polyphasic taxonomic approach. The isolates were Gram-negative, chemoheterotrophic, motile, rod-shaped cells that were psychrotolerant and moderately halophilic. Buds were produced on mother and daughter cells and on prosthecae. Prostheca formation was peritrichous and prosthecae could be branched. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the γ-Proteobacteria and are related to the genus Alteromonas, with 98·3 % sequence similarity to Alteromonas macleodii and 98·0 % to Alteromonas marina, their nearest phylogenetic neighbours. Whole-cell fatty acid profiles of the isolates were very similar and included C16 : 0, C16 : 1 ω7c, C17 : 1 ω8c and C18 : 1 ω8c as the major fatty acid components. These results support the affiliation of these isolates to the genus Alteromonas. DNA–DNA hybridization results and differences in phenotypic characteristics show that the strains represent a novel species with a DNA G+C content of 43–45 mol%. The name Alteromonas stellipolaris sp. nov. is proposed for this novel species; the type strain is ANT 69aT (=LMG 21861T=DSM 15691T). An emended description of the genus Alteromonas is given

Selective attraction of marine bacterivorous nematodes to their bacterial food

This paper explores the role of selective attraction to food in determining the spatial (micro)distribution of closely related nematode species. The attractiveness of 3 different bacterial strains to 4 species of Monhysteridae, Diplolaimelloides meyli, Diplolaimella dievengatensis, Monhystera sp. and Geomonhystera disjuncta, was studied in a multiple choice design. In our study area, the 4 nematode species considered are associated with Spartina anglica detritus decay and have partially overlapping microhabitat preferences. As they all belong to the same feeding guild, they are potential competitors for food. Each of the 4 nematode species was attracted to the bacterial strain B1, but important interspecific differences were noted in the nematodes' response to live or heat-killed bacteria, to bacteria at different tell densities or of different age, and to the filtered supernatant of B1 culture. While the responses of D. meyli to the Gram-positive bacteria Halobacillus trueperi and to the Gramnegative Escherichia coli were similar, D. dievengatensis and Monhystera sp, were preferentially attracted to H. trueperi and E. coli, respectively. This opposite preference influenced both the numbers and their relative abundances of D. dievengatensis and Monhystera sp, inside bacterial patches in experiments with a mixed 2-species nematode inoculum. Bacterial cell density strongly influenced the nematode response, with D. meyli invariably preferring the highest cell densities offered, while D. dievengatensis and Monhystera sp. had a peak response at lower cell densities. Though chemotaxis is suggested as an underlying mechanism, the nature of the nematodes' response remains unproved. The present results strongly support the importance of food patchiness in determining the heterogeneous distribution of nematodes, and extend the concept in such a way as to allow for small differences in microhabitat choice between closely related species. They also support the view that nematodes are specialist feeders, though they probably select spots where suitable food is plentiful rather than individual food particles. Finally, the present study offers a baseline for an understanding and further study of patterns of succession among nematode species associated with decaying Spartina anglica detritus in terms of highly specific relationships with different strains, growth stages, and densities of bacteria involved in the mineralization of Spartina anglica-derived organic matter

Use of <i>recA</i> as an alternative phylogenetic marker in the family <i>Vibrionaceae</i>

This study analysed the usefulness of recA gene sequences as an alternative phylogenetic and/or identification marker for vibrios. The recA sequences suggest that the genus Vibrio is polyphyletic. The high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. Photobacterium species clustered together and apparently nested within vibrios, while Grimontia hollisae was apart from other vibrios. Within the vibrios, Vibrio cholerae and Vibrio mimicus clustered apart from the other genus members. Vibrio harveyi- and Vibrio splendidus-related species formed compact separated groups. On the other hand, species related to Vibrio tubiashii appeared scattered in the phylogenetic tree. The pairs Vibrio coralliilyticus and Vibrio neptunius, Vibrio nereis and Vibrio xuii and V. tubiashii and Vibrio brasiliensis clustered completely apart from each other. There was a correlation of 0·58 between recA and 16S rDNA pairwise similarities. Strains of the same species have at least 94 % recA sequence similarity. recA gene sequences are much more discriminatory than 16S rDNA. For 16S rDNA similarity values above 98 % there was a wide range of recA similarities, from 83 to 99 %

<i>Vibrio kanaloae</i> sp. nov., <i>Vibrio pomeroyi</i> sp. nov. and <i>Vibrio chagasii</i> sp. nov., from sea water and marine animals

The taxonomic position of the fluorescent amplified fragment length polymorphism fingerprinting groups A46 (five isolates), A51 (six isolates), A52 (five isolates) and A53 (seven isolates) obtained in a previous study were further analysed through a polyphasic approach. The 23 isolates were phylogenetically related to Vibrio splendidus, but DNA-DNA hybridization experiments proved that they belong to three novel species. Chemotaxonomic and phenotypic analyses further disclosed several features that differentiate between the 23 isolates and known Vibrio species. The names Vibrio kanaloae sp. nov. (type strain LMG 20539T=CAIM 485T; EMBL accession no. AJ316193; G+C content 44·7 mol%), Vibrio pomeroyi sp. nov. (type strain LMG 20537T=CAIM 578T; EMBL accession no. AJ491290; G+C content 44·1 mol%) and Vibrio chagasii sp. nov. (type strain LMG 21353T=CAIM 431T; EMBL accession no. AJ316199; G+C content 44·6 mol%) are respectively proposed to encompass the five isolates of A46, the six isolates of A51 and the 12 isolates of A52/A53. The three novel species can be distinguished from known Vibrio species by several phenotypic features, including utilization and fermentation of various carbon sources, -galactosidase activity and fatty acid content (particularly of 12 : 0, 14 : 0, 14 : 0 iso and 16 : 0 iso)