White light thermoplasmonic activated gold nanorod arrays enable the photo-thermal disinfection of medical tools from bacterial contamination

The outspread of bacterial pathogens causing severe infections and spreading rapidly, especially among hospitalized patients, is worrying and represents a global public health issue. Current disinfection techniques are becoming insufficient to counteract the spread of these pathogens because they carry multiple antibiotic-resistance genes. For this reason, a constant need exists for new technological solutions that rely on physical methods rather than chemicals. Nanotechnology support provides novel and unexplored opportunities to boost groundbreaking, next-gen solutions. With the help of plasmonic-assisted nanomaterials, we present and discuss our findings in innovative bacterial disinfection techniques. Gold nanorods (AuNRs) immobilized on rigid substrates are utilized as efficient white light-to-heat transducers (thermoplasmonic effect) for photo-thermal (PT) disinfection. The resulting AuNRs array shows a high sensitivity change in refractive index and an extraordinary capability in converting white light to heat, producing a temperature change greater than 50 °C in a few minute interval illumination time. Results were validated using a theoretical approach based on a diffusive heat transfer model. Experiments performed with a strain of Escherichia coli as a model microorganism confirm the excellent capability of the AuNRs array to reduce the bacteria viability upon white light illumination. Conversely, the E. coli cells remain viable without white light illumination, which also confirms the lack of intrinsic toxicity of the AuNRs array. The PT transduction capability of the AuNRs array is utilized to produce white light heating of medical tools used during surgical treatments, generating a temperature increase that can be controlled and is suitable for disinfection. Our findings are pioneering a new opportunity for healthcare facilities since the reported methodology allows non-hazardous disinfection of medical devices by simply employing a conventional white light lamp

Analysis of α-synuclein species enriched from cerebral cortex of humans with sporadic dementia with Lewy bodies.

Since researchers identified α-synuclein as the principal component of Lewy bodies and Lewy neurites, studies have suggested that it plays a causative role in the pathogenesis of dementia with Lewy bodies and other 'synucleinopathies'. While α-synuclein dyshomeostasis likely contributes to the neurodegeneration associated with the synucleinopathies, few direct biochemical analyses of α-synuclein from diseased human brain tissue currently exist. In this study, we analysed sequential protein extracts from a substantial number of patients with neuropathological diagnoses of dementia with Lewy bodies and corresponding controls, detecting a shift of cytosolic and membrane-bound physiological α-synuclein to highly aggregated forms. We then fractionated aqueous extracts (cytosol) from cerebral cortex using non-denaturing methods to search for soluble, disease-associated high molecular weight species potentially associated with toxicity. We applied these fractions and corresponding insoluble fractions containing Lewy-type aggregates to several reporter assays to determine their bioactivity and cytotoxicity. Ultimately, high molecular weight cytosolic fractions enhances phospholipid membrane permeability, while insoluble, Lewy-associated fractions induced morphological changes in the neurites of human stem cell-derived neurons. While the concentrations of soluble, high molecular weight α-synuclein were only slightly elevated in brains of dementia with Lewy bodies patients compared to healthy, age-matched controls, these observations suggest that a small subset of soluble α-synuclein aggregates in the brain may drive early pathogenic effects, while Lewy body-associated α-synuclein can drive neurotoxicity

A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction

Background: Carbohydrates play a major role in cell signaling in many biological processes. We have developed a set of glycomimetic drugs that mimic the structure of carbohydrates and represent a novel source of therapeutics for endothelial dysfunction, a key initiating factor in cardiovascular complications. Purpose: Our objective was to determine the protective effects of small molecule glycomimetics against free fatty acid­induced endothelial dysfunction, focusing on nitric oxide (NO) and oxidative stress pathways. Methods: Four glycomimetics were synthesized by the stepwise transformation of 2,5­dihydroxybenzoic acid to a range of 2,5­substituted benzoic acid derivatives, incorporating the key sulfate groups to mimic the interactions of heparan sulfate. Endothelial function was assessed using acetylcholine­induced, endotheliumdependent relaxation in mouse thoracic aortic rings using wire myography. Human umbilical vein endothelial cell (HUVEC) behavior was evaluated in the presence or absence of the free fatty acid, palmitate, with or without glycomimetics (1µM). DAF­2 and H2DCF­DA assays were used to determine nitric oxide (NO) and reactive oxygen species (ROS) production, respectively. Lipid peroxidation colorimetric and antioxidant enzyme activity assays were also carried out. RT­PCR and western blotting were utilized to measure Akt, eNOS, Nrf­2, NQO­1 and HO­1 expression. Results: Ex vivo endothelium­dependent relaxation was significantly improved by the glycomimetics under palmitate­induced oxidative stress. In vitro studies showed that the glycomimetics protected HUVECs against the palmitate­induced oxidative stress and enhanced NO production. We demonstrate that the protective effects of pre­incubation with glycomimetics occurred via upregulation of Akt/eNOS signaling, activation of the Nrf2/ARE pathway, and suppression of ROS­induced lipid peroxidation. Conclusion: We have developed a novel set of small molecule glycomimetics that protect against free fatty acidinduced endothelial dysfunction and thus, represent a new category of therapeutic drugs to target endothelial damage, the first line of defense against cardiovascular disease

Into the Online Space: Outcomes for a PASS Online Pilot Across Different First Year University Subjects

Peer Assisted Study Sessions (PASS) is a student let program designed to support students transitioning into university and tackling traditionally difficult first year core subjects. The model is collaborative with student leaders facilitating activities and discussions driven by student need. Consistently research has found that students can benefit, in terms of increased grades, from attending PASS, however findings for online delivery modes are mixed. These studies have generally only compared face to face (F2F) with online modes of PASS-like programs for one subject. No study has compared different subjects from varying disciplines to investigate if the benefits of PASS online are the same for all students. PASS at UOW conducted a pilot study of synchronous online sessions, tested across three different first year university subjects. A total of 1.471 students enrolled into these subjects, with 409 attending some form of PASS. Result revealed PASS students gained significantly higher average final marks compared to students who did not attend any type of PASS, regardless off subject. However, results for PASS varied depending upon mode of delivery engaged in (F2F or online) and also with subject. Although not all differences were statistically significant, trends suggest a student/subject interaction that may vary the amount of benefit gained from PASS online formats. Possible drivers for these results are discussed as well as consideration given to cohort effects and student skills for online learning modes

Leg streamers for marking Chestnut-capped Blackbirds <i>Agelaius ruficapillus</i> in Argentina

Se condujeron estudios de campo y cautividad para evaluar el uso de las banda plásticas de colores en estudios de comportamiento del Varillero Congo (<i>Agelaius ruficapillus</i>) en una colonia reproductiva y en sitios de alimentación vecinos a la misma. En la colonia, las observaciones desde sitios ocultos muestran la utilidad del uso de las banda numeradas para la colección de datos sobre el comportamiento de los indiviudos. En los campos de arroz, si bien los números no se pudieron observar, se pudo delinear la distribución de los miembros marcados en la colonia. Se investigaron en condiciones de laboratorio y jaula, dos métodos de adhesión y dos de tipo de tintas para marcado de las banda

Peer Assisted Study Sessions (PASS) Online: Investigating the impact of an online format across different first year university subjects

As the demand for flexible learning increases, it is important to explore and expand online learning opportunities, especially in student supported learning. Peer Assisted Study Sessions (PASS) is a student led academic support program designed to help students transition into university and increase student retention. PASS is offered in traditionally challenging first year core subj ects. Due to increased popularity of PASS, along with limited space and time availability, a synchronous online format (Blackboard Collaborate) was piloted in three first year University of Wollongong (UOW) subjects in the faculties of Business, Nursing, and Psychology. The aim was to test the effectiveness of the online delivery of PASS by comparing student final grade outcomes from online cohorts with face-to-face (F2F) modes, and those students who had not attended PASS. Results demonstrated that students who attended PASS obtained significantly higher marks compared to students that did not attend PASS. Final grade outcomes for F2F versus online also varied between subjects. The different result profiles for the three subjects suggests there may be different drivers for student success in the online space. This paper presents these findings providing consideration of different factors that may influence student success, with directions for future research